Search

Your search keyword '"Patarroyo, Manuel A."' showing total 915 results
Start Over Author "Patarroyo, Manuel A." Search Limiters Peer Reviewed
915 results on '"Patarroyo, Manuel A."'

22. Immunopeptidomics of Salmonella enterica Serovar Typhimurium -Infected Pig Macrophages Genotyped for Class II Molecules.

Author

Celis-Giraldo, Carmen, Suárez, Carlos F., Agudelo, William, Ibarrola, Nieves, Degano, Rosa, Díaz, Jaime, Manzano-Román, Raúl, and Patarroyo, Manuel A.

Subjects
SALMONELLA enterica serovar typhimurium, MAJOR histocompatibility complex, MEMBRANE proteins, PEPTIDES, PORK products
Abstract

Simple Summary: Subunit vaccines based on peptides as minimal immunogenic epitopes/antigens can induce highly specific immune responses without triggering adverse reactions. Here, Salmonella enterica serovar Typhimurium peptides were identified in the immunopeptidome of porcine macrophages genotyped for different class II molecules that were stimulated with the bacteria. Immunopeptidomics and mass spectrometry approaches were used for peptide identification, along with two predictors for immunoinformatics analysis. Differences between individuals were observed concerning length, elution preferences, and elution predictions. Thirty-one bacterial peptides were identified; outer membrane protein-A and chaperonin GroEL protein had the highest number of peptides. Since proteins having the highest number of peptides have been suggested as candidate antigens for vaccines, all the bacterial peptides identified can be considered promising candidate epitopes upon determining their immunogenicity. Salmonellosis is a zoonotic infection that has a major impact on human health; consuming contaminated pork products is the main source of such infection. Vaccination responses to classic vaccines have been unsatisfactory; that is why peptide subunit-based vaccines represent an excellent alternative. Immunopeptidomics was used in this study as a novel approach for identifying antigens coupled to major histocompatibility complex class II molecules. Three homozygous individuals having three different haplotypes (Lr-0.23, Lr-0.12, and Lr-0.21) were thus selected as donors; peripheral blood macrophages were then obtained and stimulated with Salmonella typhimurium (MOI 1:40). Although similarities were observed regarding peptide length distribution, elution patterns varied between individuals; in total, 1990 unique peptides were identified as follows: 372 for Pig 1 (Lr-0.23), 438 for Pig 2 (Lr.0.12) and 1180 for Pig 3 (Lr.0.21). Thirty-one S. typhimurium unique peptides were identified; most of the identified peptides belonged to outer membrane protein A and chaperonin GroEL. Notably, 87% of the identified bacterial peptides were predicted in silico to be elution ligands. These results encourage further in vivo studies to assess the immunogenicity of the identified peptides, as well as their usefulness as possible protective vaccine candidates. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

50. Integrated genomic epidemiology and phenotypic profiling of Clostridium difficile across intra-hospital and community populations in Colombia

Author

Muñoz, Marina, Restrepo-Montoya, Daniel, Kumar, Nitin, Iraola, Gregorio, Camargo, Milena, Díaz-Arévalo, Diana, Roa-Molina, Nelly S., Tellez, Mayra A., Herrera, Giovanny, Ríos-Chaparro, Dora I., Birchenall, Claudia, Pinilla, Darío, Pardo-Oviedo, Juan M., Rodríguez-Leguizamón, Giovanni, Josa, Diego F., Lawley, Trevor D., Patarroyo, Manuel A., and Ramírez, Juan David

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results