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Summary: Repair of DNA double-strand breaks by the non-homologous end-joining pathway is initiated by the binding of Ku to DNA ends. Multiple Ku proteins load onto linear DNAs in vitro. However, in cells, Ku loading is limited to ∼1–2 molecules per DNA end. The mechanisms enforcing this limit are currently unclear. Here, we show that the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs), but not its protein kinase activity, is required to prevent excessive Ku entry into chromatin. Ku accumulation is further restricted by two mechanisms: a neddylation/FBXL12-dependent process that actively removes loaded Ku molecules throughout the cell cycle and a CtIP/ATM-dependent mechanism that operates in S phase. Finally, we demonstrate that the misregulation of Ku loading leads to impaired transcription in the vicinity of DNA ends. Together, our data shed light on the multiple mechanisms operating to prevent Ku from invading chromatin and interfering with other DNA transactions.

A major strategic priority of Barito Utara Regency is maternal and neonatal health issues since the maternal mortality rate (MMR) and infant mortality rate (IMR) have fluctuated with an increasing trend from 2016 to 2022. This case discusses innovation at Kandui Public Health Centre in Gunung Timang Subdistrict as the only thriving area that brought down IMR and MMR to zero for five consecutive years (2019-2023). Despite the benefits of JESAYA (Jembatan Bersalin Sayang Anak/Child-Friendly Maternity Bridge) innovation being recognised, its adoption rate by other public health centres remained low. This study aims to identify the intrinsic factors influencing the diffusion of JESAYA. To study these issues adequately, a qualitative case study with a phenomenological approach was selected using various data collection techniques, including interviews with nine research subjects, observation, and documentation. The results suggest that adopting innovation is not straightforward, and its intrinsic factors play a crucial role in its success. Additionally, it appears that the process of adopting JESAYA has a social component, which collegial interactions and discussions may facilitate.

Nicotinium Nitrate Monohydrate (NNM) was grown by slow evaporation method. The arrangment of the molecules and hydrogen bonding intractionsin the grown crystal were analyzed using single crystal XRD. The structure, functional groups (vibrational analysis) and electronic properties (Mulliken and HOMO-LUMO analysis) were calculated by the density functional theory (DFT)/B3LYP/wB97XD method using 6–311++G (d,p) basis set. Also, the binding energy and the effect of basis set superposition error (BSSE) on the ring motifs have been investigated. The intermolecular interactions and charge delocalization density of NNM crystal have been analyzed using NBO 3.1 program. The strength of intermolecular interaction was further analyzed by 3D Hirshfeld surface analysis using fingerprint plot. Improved solubility of the title crystal NNM shows an enhanced physio-chemical activity compared to pure vitamin B3 (Nicotinic acid). The antibacterial activity of NNM crystal increased against Staphylococcus aureus a gram-positive bacteria when compared with pure nicotinic acid.

Meiotic sex chromosome inactivation is an essential event in male germ cell development, which is directed by DNA damage response signaling independent of Xist RNA to silence the transcription activity of the sex chromosomes. However, the specific mechanism of establishment and maintenance of meiotic chromosome silencing is still unclear. Here we identify the HSF5 as a testicular specific protein and the expression of which was at the onset of meiosis pachytene stage to round sperm. When the function of the HSF5 was lost, meiosis sex chromosome remodeling and silencing fail, followed by activation of CHK2 checkpoint leads to germ cell apoptosis. Furthermore, we found that SMARCA4 in the linking the HSF5 to MSCI and uncover additional factors with meiotic sex chromosome remodeling. Together, our results demonstrate a requirement for HSF5 activity in spermatogenesis and suggest a role for the mammalian HSF5-SMARCA4 in programmed meiotic sex chromosome remodeling and silencing events that take place during meiosis.

Cagar budaya merupakan warisan budaya yang bisa bersifat kebendaaan dan perlu dilestarikan karena memiliki nilai penting bagi sejarah. Perkebunan Kopi De Karanganyar merupakan salah satu wisata cagar budaya yang terletak Kabupaten Blitar. Perkebunan ini didirikan tahun 1874 oleh warga Belanda bernama H.J. Velsink. Perkebunan kopi ini merupakan salah satu penghasil kopi hitam di Blitar. Perkebunan ini memiliki beberapa atraksi wisata didalamnya yang bisa dikunjungi oleh wisatawan. Atraksi wisata yang tersedia antara lain: Koffie Boomstaart, Roemah Lodji, Museum Nugroho, dan Pabrik. Perkebunan kopi ini merupakan salah satu destinasi wisata yang tersedia dalam paket wisata Saya Tour. Keberadaan wisata cagar budaya ini masih belum dikelola dengan optimal oleh Saya Tour, mitra kegiatan pengabdian. Berdasarkan kondisi mitra kegiatan pengabdian dan peluang yang bisa diberikan ke mitra, maka tujuan kegiatan pengabdian ini yaitu untuk memberikan pengetahuan dan wawasan terhadap mitra terkait dengan pemanfaatan teknologi digital untuk memetakan dan mengemas wisata cagar budaya. Metode yang digunaan dalam kegiatan pengabdian ini yaitu metode konsultasi dan pelatihan. Kegiatan telah dilaksanakan sesuai dengan target dan telah memberikan solusi atas permasalahan mitra dengan memberikan beberapa rekomendasi, antara lain pemetaan lokasi wisata berbasis digital, pengemasan wisata memanfaatkan teknologi multimedia dan virtual tourism

El artículo presenta los resultados de la evaluación realizada a estudiantes que ingresan a la carrera de Pedagogía en Educación General Básica, de dos instituciones universitarias regionales pertenecientes al Consejo de Rectores en Chile, en habilidades de razonamiento y conocimiento matemático y comprensión lectora. Corresponde a una evaluación diagnóstica de tipo descriptiva y comparativa de corte transversal, a una población compuesta por 80 alumnos, a quienes se les aplicó instrumentos debidamente validados con un Alfa de Cronbach α=0.82 en Matemática y de α=0.64 en Comprensión Lectora. Para el análisis de la información, se utilizaron métodos descriptivos univariados y multivariados. Los resultados evidencian, en general, un dominio descendido en ambas habilidades o por debajo de lo esperado al ingreso de la Educación Superior, especialmente en las habilidades matemáticas.Based on a comparative cross-assessment diagnosis conducted in two Council of Rectors (CRUCH) universities in southern Chile in 2009, this paper shows the results of Math reasoning and reading comprehension skills assessment of entrant students from the Primary School Teaching Program. Data for this paper comes from a test with Cronbach's Alpha reliability of 0,82 in Math and 0,64 in Reading Comprehension, which was applied to a sample of 80 students. Data analysis required one-variable and multivariable descriptive methods and the results dramatically showed either a low command of both skills or far lower than expected performance for entrant students from the Primary School Teaching Program, especially in Maths.

This paper considers schematically the various discourses through which popular music history is understood. My proposal is that five accounts of musical history (the business model, the musicological model, the sociological model, the historical model and the art history model) are commonly deployed in popular music discourse. One implies, superficially at least, that popular music evolves, gets better; four implies that, at least in the longer term, it does not. The concept of ′progress′ is shown to be problematic.