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8. A simple and effective method for detecting precipitated proteins in MALDI−TOF MS.

Author

Oshikane, Hiroyuki, Watabe, Masahiko, and Nakaki, Toshio

Subjects
*PROTEINS, *LIFE sciences, *MASS spectrometry, *MOLECULAR weights, *CINNAMIC acid derivatives
Abstract

MALDI−TOF MS has developed rapidly into an essential analytical tool for the life sciences. Cinnamic acid derivatives are generally employed in routine molecular weight determinations of intact proteins using MALDI−TOF MS. However, a protein of interest may precipitate when mixed with matrix solution, perhaps preventing MS detection. We herein provide a simple approach to enable the MS detection of such precipitated protein species by means of a “direct deposition method” -- loading the precipitant directly onto the sample plate. It is thus expected to improve routine MS analysis of intact proteins. [ABSTRACT FROM AUTHOR]

Published
2018
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