1. Terabase-Scale Coassembly of a Tropical Soil Microbiome
- Author
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Robert Riley, Robert M. Bowers, Antonio Pedro Camargo, Ashley Campbell, Rob Egan, Emiley A. Eloe-Fadrosh, Brian Foster, Steven Hofmeyr, Marcel Huntemann, Matthew Kellom, Jeffrey A. Kimbrel, Leonid Oliker, Katherine Yelick, Jennifer Pett-Ridge, Asaf Salamov, Neha J. Varghese, and Alicia Clum
- Subjects
metagenomics ,terabase ,tropical soil ,redox ,rare biosphere ,Microbiology ,QR1-502 - Abstract
ABSTRACT Petabases of environmental metagenomic data are publicly available, presenting an opportunity to characterize complex environments and discover novel lineages of life. Metagenome coassembly, in which many metagenomic samples from an environment are simultaneously analyzed to infer the underlying genomes’ sequences, is an essential tool for achieving this goal. We applied MetaHipMer2, a distributed metagenome assembler that runs on supercomputing clusters, to coassemble 3.4 terabases (Tbp) of metagenome data from a tropical soil in the Luquillo Experimental Forest (LEF), Puerto Rico. The resulting coassembly yielded 39 high-quality (>90% complete,
- Published
- 2023
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