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Purpose The clinical diagnosis of pulmonary sarcoidosis is based on the presence of noncaseating granulomas in an appropriate clinical setting with either bilateral hilar adenopathy and/or parenchymal infiltrates. Lymphocytosis with an increased CD4/CD8 T cell ratio in bronchoalveolar lavage fluid is supportive. We evaluated the diagnostic accuracy of a predictive binary logistic regression model in sarcoidosis based on sex, age, and bronchoalveolar lavage fluid cell profile with and without the inclusion of HLA-DR.sup.+ CD8.sup.+ T cells and natural killer T-cell fractions. Methods A retrospective analysis of differential cell counts and lymphocyte phenotypes by flow cytometry in bronchoalveolar lavage was performed in 183 patients investigated for possible diffuse parenchymal lung disease. A logistic regression model with age, sex, lymphocyte fraction, eosinophils, and CD4/CD8 ratio in bronchoalveolar lavage fluid (basic model) was compared with a final model, which also included fractions of HLA-DR.sup.+ CD8.sup.+ T cells and natural killer T cells. Diagnostic accuracy of the two models was assessed by receiver operating characteristic (ROC) curves. Results The area under the ROC curve for the basic and final model was 0.898 [95 % confidence interval (CI) 0.852-0.945] and 0.937 (95 % CI 0.902-0.972), respectively, p = 0.008. Conclusions Assessment of HLA-DR.sup.+ CD8.sup.+ T cell and natural killer T-cell fractions may improve diagnostic accuracy and further strengthen the importance of bronchoalveolar lavage in the diagnostic workup of sarcoidosis. Electronic supplementary material The online version of this article (doi:10.1007/s00408-013-9527-8) contains supplementary material, which is available to authorized users., Author(s): A. Tøndell[sup.1] [sup.2] [sup.4] , A. D. Rø[sup.2] , A. Åsberg[sup.3] , M. Børset[sup.2] [sup.4] , T. Moen[sup.2] [sup.5] , M. Sue-Chu[sup.1] [sup.6] Author Affiliations: (1) Department of Thoracic [...]

Antinuclear antibodies (ANA) were demonstrated in 3 out of 10 Gordon setters with symmetrical lupoid onychodystrophy and in 5 out of 13 Gordon setters with black hair follicular dysplasia. Two dogs showed both symmetrical lupoid onychodystrophy and black hair follicular dysplasia, and one of these was ANA positive. The results suggest that symmetrical lupoid onychodystrophy and black hair follicular dysplasia in the Gordon setter might be autoimmune diseases that are pathogenetically related, which might indicate a common genetic predisposition.

Ruminal fiber digestion is often decreased by supplementation of readily fermentable carbohydrates. Five ruminally cannulated beef steers were used in a 5 x 5 Latin square design to evaluate the effects of grain type (corn vs barley) and ruminal buffer (Na sesquicarbonate; 0 vs 1.2% of dietary DM) on ruminal digestion and fermentation in wheat straw-based diets. Grain supplements were 30% of the dietary DM. The 2 x 2 + 1 factorial arrangement of treatments included a control supplement that consisted primarily of soybean meal. Diets were fed once daily and were formulated to be 10% CP (DM basis). In situ DM (ISDMD) and NDF (ISNDFD) disappearance of wheat straw was measured at 0, 8, 16, 24, 32, 40, and 96 h of incubation. To examine the effects of time after supplementation, 8-h incubations were performed at 0 to 8, 4 to 12, 8 to 16, 12 to 20, and 16 to 24 h after supplementation. Corn diets resulted in lower (P |is less than~ .05) ISDMD for the 12- to 20- and 16- to 24-h periods than did barley diets. Averaged across 8-h intervals, the control treatment had greater ISDMD (P |is less than~ .01) and ISNDFD (P |is less than~ .05) than the grain treatments. Treatment differences were not observed for ISDMD and ISNDFD after 8 h of incubation. Ruminal fluid pH for barley diets was greater at 0, 16, and 20 h and less at 4 h after feeding than for corn diets (treatment x hour; P |is less than~ .01). Propionate concentration was greater (P |is less than~ .05) for corn than for barley diets. Buffer addition increased acetate concentration, acetate:propionate ratio, and total VFA concentration for barley diets, whereas a reverse trend was observed for corn diets (grain type x buffer; P |is less than~ .10). Results of this study demonstrate only subtle changes in ruminal fiber digestion and fermentation as affected by grain type and buffer addition.

Two corn hybrids (Pioneer 3377 and 3389) with similar total plant and grain yield characteristics were evaluated to determine potential differences in nutritive value of the whole plant and stover. Hybrids were grown in plots at four different locations in Idaho in 1988 and 1989 for laboratory evaluation. Samples from the plots were stored fresh or ensiled with or without a microbial inoculant for 60 d in laboratory silos. Whole-plant samples of 3377 had a lower (P |is less than~ .01) percentage of NDF (42.7 vs 48.1), ADF (26.3 vs 30), hemicellulose (16.2 vs 18.2), cellulose (21.4 vs 24), and lignin (3.4 vs 3.8). Similar differences, but of larger magnitude, were observed in stover samples. Ruminal in situ (24 h) and two-stage IVDMD of whole-plant and stover samples were greater (P |is less than~ .01) for 3377 than for 3389. Lower fiber content and greater degradability for 3377 was not due to greater grain content; 3377 actually had numerically lower grain as a percentage of whole plant than 3389 (41 vs 44). Ensiling reduced (P |is less than~ .05) NDF, hemicellulose, and cellulose content compared with fresh samples; however, no effect (P |is greater than~ .10) due to inoculant was noted. Hybrid x storage interactions were not observed (P |is greater than~ .10). Inoculation and hybrid did not affect (P |is greater than~ .10) organic acid concentration of the silages. In 1989 silages from 3377 and 3389 were harvested to evaluate diets containing 65% (DM basis) corn silage in steer digestion and growth trials. Digestible energy and digestibility of DM and CP were greater (P |is less than~ .05), and the trend (P |is greater than~ .05) was for greater NDF and ADF digestibility for the 3377 than for the 3389 diet. In a growth trial, steers fed the 3377 silage diet had greater (P |is less than~ .05) daily gain (1.09 vs 1.01 kg/d), consumed numerically less (P = .09) DM, and had improved (P |is less than~ .01) feed efficiency (6.75 vs 7.49 feed:gain) compared with steers fed the 3389 silage diet. Results of the experiments indicate that large differences in feed value of corn silage may exist among hybrids with similar grain content.

Ion milling has been used to convert molecular beam epitaxy vacancy-doped Cd[sub x]Hg[sub 1-x]Te from p- to n-type. Electron beam induced current and remote electron beam induced current (REBIC) measurements have been performed to study the pn junction depth and lateral extension dependence on the milling time, milling current, and vacancy concentration. The conversion depth is linear with the milling time and current and inversely proportional to the vacancy concentration in layers thinner than 10 μm. This shows that filling of Hg vacancies in this region during conversion is limited by the rate of supply of extra Hg from the milling. The lateral extension also increases linearly with the milling time, the ratio of the lateral extension to the depth being ∼0.5. One can therefore use REBIC on the top surface to determine the junction depth, which greatly simplifies the measurement and does not destroy the diodes. © 2002 American Institute of Physics. [ABSTRACT FROM AUTHOR]

In this study, we describe the development and characterization of the first high-density single nucleotide polymorphism ( SNP) genotyping array for rainbow trout. The SNP array is publically available from a commercial vendor (Affymetrix). The SNP genotyping quality was high, and validation rate was close to 90%. This is comparable to other farm animals and is much higher than previous smaller scale SNP validation studies in rainbow trout. High quality and integrity of the genotypes are evident from sample reproducibility and from nearly 100% agreement in genotyping results from other methods. The array is very useful for rainbow trout aquaculture populations with more than 40 900 polymorphic markers per population. For wild populations that were confounded by a smaller sample size, the number of polymorphic markers was between 10 577 and 24 330. Comparison between genotypes from individual populations suggests good potential for identifying candidate markers for populations' traceability. Linkage analysis and mapping of the SNPs to the reference genome assembly provide strong evidence for a wide distribution throughout the genome with good representation in all 29 chromosomes. A total of 68% of the genome scaffolds and contigs were anchored through linkage analysis using the SNP array genotypes, including ~20% of the genome assembly that has not been previously anchored to chromosomes. [ABSTRACT FROM AUTHOR]

OBJECTIVE Continuous beta-cell rest with diazoxide preserves residual endogenous insulin production in type 1 diabetes. However, side effects have hampered therapeutic usefulness. In a double-blind study, we tested whether lower, intermittent dosing of diazoxide had beneficial effects on insulin production, metabolic control, and autoimmunity markers in the absence of side effects. RESEARCH DESIGN AND METHODS Forty-one newly diagnosed type 1 diabetic patients were randomized to 6 months of treatment with placebo or 100 mg diazoxide at bedtime. A1C, C-peptide (fasting and glucagon stimulated), and FoxP3(+) regulatory T-cells (Tregs) were measured. Patients were followed for 6 months after intervention. RESULTS Of six dropouts, three were due to perceived side effects; one subject in the diazoxide group experienced rash, another dizziness, and one in the placebo group sleep disturbance. Adverse effects in others were absent. Diazoxide treatment reduced A1C from 8.6% at baseline to 6.0% at 6 months and 6.5% at 12 months. Corresponding A1C value in the placebo arm were 8.3, 7.3, and 7.5% (P < 0.05 for stronger reduction in the diazoxide group). Fasting and stimulated C-peptide decreased during 12 months similarly in both arms (mean -0.30 and -0.18 nmol/l in the diazoxide arm and -0.08 and -0.09 nmol/l in the placebo arm). The proportion of Tregs was similar in both arms and remained stable during intervention but was significantly lower compared with nondiabetic subjects. CONCLUSIONS Six months of low-dose diazoxide was without side effects and did not measurably affect insulin production but was associated with improved metabolic control. [ABSTRACT FROM AUTHOR]

We report a case of aberrant expression of the T cell antigen CD8 in a patient with B cell chronic lymphocytic leukaemia (B-CLL). A 62-year-old Caucasian female with several enlarged lymph nodes and suspected to have B-CLL was referred to our laboratory for routine immunophenotyping. Peripheral blood cell count showed moderate leucocytosis without other abnormalities. The dual-colour flow cytometric analysis showed a typical B-CLL phenotype (CD45 +, CD19 +, κ +, λ -, CD20 +, CD23 +, IgM +, HLA-DR +, CD5/CD19 +, CD3 -). In addition, aberrant expression of the T cell marker CD8 was found, present on approximately 64% of the leukemic cells. This is a rare even, the significance and nature of this aberration has not yet been fully determined. In this case, our patient had a rapid response to treatment with a remarkable reduction in the number of leukemic cells only two weeks after beginning treatment. [ABSTRACT FROM AUTHOR]

In order to find potential correlations between HLA class II alleles and anti-SS-A,-SS-B, -Sm and anti-snRNP responses among Norwegian patients with systemic lupus erythematosus (SLE), HLA-DRB1, -DRB3*0101, -DQA1 and -DQB1 alleles were determined by DNA typing 50 patients and 108 controls. HLA distributions were analysed in the following autoantibody subgroups: anti-SS-A with -SS-B, anti-SS-A without -SS-B, anti-snRNP without -Sm, anti-SS-A without -snRNP and anti-snRNP without -SS-A. The autoantibodies were detected by EIA (enzyme immunuassay). Patients with anti-SS-A and -SS-B had significantly increased frequencies of DRB1*03, DRB3*0101, DQA1*0501, DQB1*0201 (in linkage disequilibrium) versus controls and versus patients without anti-SS-A and -SS-B. No differences in HLA distribution were found when the group with anti-SS-A alone was compared to the group with anti-SS-A and concomitant -SS-B. Comparing the groups with and without anti-SS-A and -SS-B, the highest RR were found for the alleles DRB1*03, DRB3*0101, DQB1*0501, DQB1*0201 (in linkage disequilibrium) with RR: 16.8, 5.0, 19.6, 10.3, respectively, P < 0.05). RR for DQw2/DQw6 heterozygotes was 3.5 (Ns.), and RR for cases having DQα molecules with glutamine in position 34 and DQβ molecules with leucine in position 26 on both chains was 6.3 (P < 0.05). No HLA associations were observed in the group with anti-snRNP without concomitant -Sm or without concomitant -SS-A. These results show that production of anti-SS-A and -SS-B is associated to the HLA alleles DRB1*03, DRB3*0101, DQA1*0501, DQB1*0201, and that this haplotype shows stronger correlation to these responses than DQw2/DQw6 heterozygosity or HLA molecules having glutamine in position 34 (DQα) and leucine in position 26 (DQβ). The failure to observe any correlation with DRB1*15,16 (DP,2) in the group with anti-SS-A alone may demonstrate ethnic differences concerning this response. The failure to identify any HLA associations for the anti-snRNP response may reflect the heterogeneity of the molecules that constitute this antigen. [ABSTRACT FROM AUTHOR]

Polyclonal activation of B-lymphocytes accompanies many retroviral infections. Friend derived murine immunosuppressive virus (Fd-MIV) is a non-defective murine retrovirus which was isolated from T-helper cells from mice infected with the acute transforming retrovirus Friend leukaemia complex (FLC). In contrast to FLC. Fd-MIV does not induce acute transformation of lymphoid and erythroid cells but causes immunosuppression and lymphadenopathy in adult NMRI mice. The effect of Fd-MIV infection on B-lymphocytes was studied. Fd-MIV infection led to a persistent hypergammaglobulinemy with a significant increase in the level of circulating IgG. IgM and immune complexes. In the spleen and lymph nodes. B-lymphocyte proliferation was found. Parallel to the development of hypergammaglobulinemy. autoantibodies to a variety of nuclear and other autoantigens was detected. In conclusion, the Fd-MIV infection leads to a B-lymphocyte dysfunction that has many parallels with AIDS. Furthermore, the Fd- MIV infection seems to represent an example of an autoimmune condition caused by an exogenous infectious agent. [ABSTRACT FROM AUTHOR]