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Heterocyclic compounds have great importance in the medical and industrial fields. Imidazole compounds and salts are the most widespread and effective of these compounds. Therefore, in this research, we prepare a number of mono- and di-substituted derivatives of imidazole and its salts. The prepared compounds are characterized using ¹H-NMR, 13C-NMR, SEM, and IR techniques. In addition, the biological activity against Escherichia coli and Staphylococcus aureus bacteria is studied for the prepared compounds. As revealed, the prepared salts are more biologically effective. [ABSTRACT FROM AUTHOR]