1. Detection of the TMPRSS2-ETS fusion gene in prostate carcinomas: retrospective analysis of 55 formalin-fixed and paraffin-embedded samples with clinical data
- Author
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Cécile Rouzier, Jean Amiel, Jean-François Michiels, Laure Valério, Juliette Haudebourg, Florence Pedeutour, Xavier Carpentier, Laboratory of Solid Tumors Genetics, Nice University Hospital, Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Laboratoire d'Anatomo-Pathologie, Hôpital Pasteur [Nice] (CHU), Laboratoire d'Urologie, Department of Public Health, and Hôpital l'Archet
- Subjects
Male ,Cancer Research ,Oncogene Proteins, Fusion ,Oncogene Proteins ,MESH: Paraffin Embedding ,Chromosomal translocation ,MESH: Formaldehyde ,law.invention ,Fusion gene ,MESH: Aged, 80 and over ,0302 clinical medicine ,law ,Prostate ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,MESH: Fixatives ,MESH: Serine Endopeptidases ,Polymerase chain reaction ,MESH: Aged ,Aged, 80 and over ,0303 health sciences ,MESH: Middle Aged ,Paraffin Embedding ,Reverse Transcriptase Polymerase Chain Reaction ,Serine Endopeptidases ,MESH: Transcription Factors ,Middle Aged ,3. Good health ,DNA-Binding Proteins ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Adenocarcinoma ,Adenovirus E1A Proteins ,PCA3 ,MESH: Proto-Oncogene Proteins c-ets ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Biology ,Fixatives ,03 medical and health sciences ,Antigen ,Formaldehyde ,Proto-Oncogene Proteins ,Genetics ,medicine ,Humans ,Molecular Biology ,Aged ,Retrospective Studies ,030304 developmental biology ,MESH: Humans ,Proto-Oncogene Proteins c-ets ,MESH: Adenocarcinoma ,Prostatic Neoplasms ,MESH: Retrospective Studies ,medicine.disease ,MESH: Adenovirus E1A Proteins ,MESH: Male ,MESH: Proto-Oncogene Proteins ,[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics ,MESH: Prostatic Neoplasms ,Immunology ,Cancer research ,Feasibility Studies ,MESH: Feasibility Studies ,MESH: DNA-Binding Proteins ,MESH: Oncogene Proteins, Fusion ,Transcription Factors - Abstract
The recent identification of fusion genes involving ETS family members in human prostate adenocarcinoma has confirmed the hypothesis that recurrent specific aberrations such as fusion genes may be as frequent in epithelial tumors as they are in leukemias and sarcomas. However, reciprocal translocations with fusion genes are often not detectable in carcinomas by conventional karyotyping because of additional complex chromosomal abnormalities. We retrospectively analyzed a large series of formalin-fixed, paraffin-embedded samples including 55 prostate carcinomas and 11 benign prostate tumors. We identified the fusion gene TMPRSS2-ERG by reverse-transcriptase polymerase chain reaction (RT-PCR) in 40/55 carcinomas (72%). Our study demonstrates that the detection of ETS fusion gene by RT-PCR is feasible on formalin-fixed and paraffin-embedded samples. No significant association between the presence of the fusion gene and any clinical feature, such as preoperative serum prostate-specific antigen (PSA) level (PSA>20 or PSA< or =20), pTNM stage including capsule invasion, seminal vesicle invasion, and lymph nodes metastases, or recurrence was observed in our series.
- Published
- 2008
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