Search

Your search keyword '"Lu, Congming"' showing total 160 results
Start Over Author "Lu, Congming" Search Limiters Peer Reviewed
160 results on '"Lu, Congming"'

9. Sucrose phosphate synthase 8 is required for the remobilization of carbon reserves in rice stems during grain filling.

Author

Ma, Mingyang, Zhu, Tong, Cheng, Xiuyue, Li, Mengyu, Yuan, Guoliang, Li, Changbao, Zhang, Aihong, Lu, Congming, Fang, Ying, and Zhang, Yi

Subjects
SUCROSE, RNA sequencing, PHOSPHATES, CARBON, STARCH
Abstract

Carbon reserve remobilization in stems is closely related to rice grain filling. Sucrose phosphate synthase (SPS) is highly associated with carbon reserve remobilization. In this study, we investigated the expression pattern of SPS genes in various rice tissues, and found that SPS8 is the major SPS isoform in rice stems during the grain-filling stage. We then constructed sps8 mutants using the CRISPR/Cas9 system. The SPS activity of the sps8 mutants was markedly reduced in the stems. In addition, the sps8 mutants exhibited significant starch accumulation in stems. 14C-labelling experiments revealed that the remobilization of non-structural carbohydrates from rice stems to grains was impaired in the sps8 mutants. In the sps8 mutants, grain filling was delayed and yield decreased by 15% due to a reduced percentage of ripened grains. RNA sequencing and quantitative PCR analyses indicated that the genes involved in starch synthesis and degradation were up-regulated in the sps8 mutant stems. In addition, the activity of the enzymes involved in starch synthesis and degradation was increased in the sps8 stems. These results demonstrate that SPS8 is required for carbon reserve remobilization from rice stems to grains, and that its absence may enhance 'futile cycles' of starch synthesis and degradation in rice stems. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

11. Chloroplast retrograde signal regulates flowering

Author

Feng, Peiqiang, Guo, Hailong, Chi, Wei, Chai, Xin, Sun, Xuwu, Xu, Xiumei, Ma, Jinfang, Rochaix, Jean-David, Leister, Dario, Wang, Haiyang, Lu, Congming, and Zhang, Lixin

Published
2016

41. Autophagy targets Hd1 for vacuolar degradation to regulate rice flowering.

Author

Hu, Zhi, Yang, Zhipan, Zhang, Yi, Zhang, Aihong, Lu, Qingtao, Fang, Ying, and Lu, Congming

Abstract

Flowering time (heading date) is a critical agronomic trait that determines the yield and regional adaptability of crops. Heading date 1 (Hd1) is a central regulator of photoperiodic flowering in rice (Oryza sativa). However, how the homeostasis of Hd1 protein is achieved is poorly understood. Here, we report that the nuclear autophagy pathway mediates Hd1 degradation in the dark to regulate flowering. Loss of autophagy function results in an accumulation of Hd1 and delays flowering under both short-day and long-day conditions. In the dark, nucleus-localized Hd1 is recognized as a substrate for autophagy and is subjected to vacuolar degradation via the autophagy protein OsATG8. The Hd1-OsATG8 interaction is required for autophagic degradation of Hd1 in the dark. Our study reveals a new mechanism by which Hd1 protein homeostasis is regulated by autophagy to control rice flowering. Our study also indicates that the regulation of flowering by autophagic degradation of Hd1 orthologs may have arisen over the course of mesangiosperm evolution, which would have increased their flexibility and adaptability to the environment by modulating flowering time. Hd1 is a core regulator of photoperiodic flowering in rice, but the regulatory mechanisms of Hd1 protein homeostasis remain unclear. This study demonstrates that nucleus-localized Hd1 is recognized as a substrate for autophagy in the dark and is subjected to vacuolar degradation via the autophagy protein OsATG8, revealing a mechanism by which autophagy mediates Hd1 degradation to control rice flowering. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

47. Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism.

Author

Miao, Rui, Siao, Wei, Zhang, Na, Lei, Zuliang, Lin, Deshu, Bhalerao, Rishikesh P., Lu, Congming, and Xu, Weifeng

Subjects
MICROTUBULES, ABSCISIC acid, PROTEIN microarrays, TISSUE arrays, ROOT growth, SOIL moisture
Abstract

Root hydrotropism refers to root directional growth toward soil moisture. Cortical microtubule arrays are essential for determining the growth axis of the elongating cells in plants. However, the role of microtubule reorganization in root hydrotropism remains elusive. Here, we demonstrate that the well-ordered microtubule arrays and the microtubule-severing protein KATANIN (KTN) play important roles in regulating root hydrotropism in Arabidopsis. We found that the root hydrotropic bending of the ktn1 mutant was severely attenuated but not root gravitropism. After hydrostimulation, cortical microtubule arrays in cells of the elongation zone of wild-type (WT) Col-0 roots were reoriented from transverse into an oblique array along the axis of cell elongation, whereas the microtubule arrays in the ktn1 mutant remained in disorder. Moreover, we revealed that abscisic acid (ABA) signaling enhanced the root hydrotropism of WT and partially rescued the oryzalin (a microtubule destabilizer) alterative root hydrotropism of WT but not ktn1 mutants. These results suggest that katanin-dependent microtubule ordering is required for root hydrotropism, which might work downstream of ABA signaling pathways for plant roots to search for water. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results