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Premise: Volatile emissions from flowers and fruits play a key role in signalling to animals responsible for pollination and seed dispersal. Here, we investigated the pollination biology and chemical ecology of reproduction in Apodolirion buchananii, an African amaryllid that flowers in a leafless state soon after grassland vegetation is burnt in the dry late‐winter season. Methods: Pollinators were identified through field collection and pollen loads were quantified. Floral traits including spectral reflectance and scent chemistry were documented. Bioassays using cup traps were used to test the function of floral volatiles. Fruiting biology was investigated using controlled hand‐pollination experiments and chemical analysis of fruit scent. Seed germination was scored in greenhouse trials. Seed dispersal was monitored using observations and camera trapping. Results: The sweetly scented white flowers of A. buchananii are pollen‐rewarding and pollinated mainly by a diverse assemblage of bees. Cup‐trap experiments demonstrated that pollinators are attracted to phenylacetaldehyde, the dominant volatile in the floral scent. Plants are shown to be self‐incompatible, and the fleshy fruits were found to emerge from the soil six months after pollination during the peak of the summer rains. Fruits emit a diverse blend of aliphatic and aromatic esters and contain large fleshy recalcitrant seeds which germinate within days of fruits splitting open. Seed dispersal by ants was recorded. Conclusions: This first account of the reproductive biology of a species in the genus Apodolirion highlights an outcrossing mating system involving bees attracted to color and scent as well as the unusual fruiting biology and ant‐mediated system of seed dispersal. [ABSTRACT FROM AUTHOR]

Mutualisms between nectarivorous birds and the plants they pollinate are functionally diverse. Nectarivorous birds which hover while feeding (the majority of hummingbirds) tend to have straight bills, while those that perch while feeding (some hummingbirds and almost all passerine nectarivores) tend to have decurved bills. Sunbirds typically use their curved bills to feed in an arc from a perching position and we thus predicted that they would prefer, and feed most efficiently on, flowers that are curved towards a perching position. To test this, we examined the responses of sunbirds to model flowers differing in curvature (straight or curved), orientation (facing upwards or downwards), and availability of a top perch (present or absent). Birds did not show preferences among model flower types in terms of number of landings to feed or number of probes. In general they preferred to use perches above model flowers, particularly those that curved upwards, but they tended to perch below model flowers that curve downwards and in such cases also took the least time to insert their bills. These results are consistent with the idea that perching birds with curved beaks will feed most efficiently from flowers that are curved towards the perching position. We discuss the implications of these results for the evolution of floral architecture, including provision of perches. [ABSTRACT FROM AUTHOR]

Seeds of many Amaryllidaceae are unorthodox (recalcitrant) and toxic, and cannot survive ingestion, yet are packaged in brightly colored fruits suggestive of zoochory. Seed dispersal and germination of the understory amaryllid, Clivia miniata, were investigated in KwaZulu‐Natal, South Africa. Motion‐activated cameras revealed that samango monkeys (Cercopithecus mitis labiatus) are the primary disperser of C. miniata seeds. They eat the mesocarp and, to a lesser extent, the exocarp, and spit the large (13 mm diameter) seeds whole and cleaned onto the forest floor. Most seeds were dispersed farther than 1 m from the parent. Experimental removal of the fruit pulp had a small positive effect on the rate of seed germination, but did not affect subsequent seedling growth rates. The main advantages of monkey dispersal of Clivia seeds appear to be short‐distance dispersal away from the dense foliage of clumped parent plants and occasional long‐distance dispersal through cheek‐pouching behavior. [ABSTRACT FROM AUTHOR]

Late-acting (ovarian) self-incompatibility, characterized by minimal or zero seed production following self-pollen tube growth to the ovules, is expected to show phylogenetic clustering, but can otherwise be difficult to distinguish from early-acting inbreeding depression. In Amaryllidaceae, late-acting self-incompatibility has been proposed for Narcissus ( Narcisseae) and Cyrtanthus ( Cyrtantheae). Here, we investigate whether it occurs in the horticulturally important genus Clivia ( Haemantheae) and test whether species in this genus experience ovule discounting in wild populations. Seed-set results following controlled hand pollinations revealed that Clivia miniata and C. gardenii are largely self-sterile. Self- and cross-pollinated flowers of both species had similar proportions of pollen tubes entering the ovary, and those of C. gardenii also did not differ in the proportions of pollen tubes that penetrated ovules, thus ruling out classical gametophytic self-incompatibility acting in the style, but not early inbreeding depression. Flowers that received equal mixtures of self- and cross-pollen set fewer seeds than those that received cross-pollen only, but it was unclear whether this effect was a result of ovule discounting or interactions on the stigma. The prevention of self-pollination by the emasculation of either single flowers or whole inflorescences in wild populations did not affect seed set, suggesting that ovule discounting is not a major natural limitation on seed production. Flowers typically produce one to three large fleshy seeds from approximately 16 available ovules, even when supplementally hand pollinated, suggesting that fecundity is mostly resource limited. The results of this study suggest that Clivia spp. are largely self-sterile as a result of either a late-acting self-incompatibility system or severe early inbreeding depression, but ovule discounting caused by self-pollination is not a major constraint on fecundity. © 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 175, 155-168. [ABSTRACT FROM AUTHOR]