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This paper presents an analog baseband (ABB) circuit in a 0.13 μm SiGe technology for transmitter leakage cancellation and close-in blocker suppressions in fully duplex (FD) frequency-modulated continuous-wave (FMCW) radar. This ABB comprises a programmable gain amplifier (PGA) and a cascaded LPF/Notch hybrid, which incorporates a closed-loop (CL) reconfigurable low-pass filter (LPF) and an open-loop (OL) tunable notch filter. The adopted key topologies include active-RC bi-quads and Gm-C ones. In an FD FMCW transceiver, Tx leakage and close-in blockers are difficult to be eliminated in the RF domain, especially when leakage/blockers are very close to desired signals or even in-band in the frequency domain. This LPF/notch hybrid is proposed to solve this issue. The LPF and PGA provide bandwidth (BW)/gain programmability, while the Gm-C bi-quad provides adaptable center frequency for a notch filter. With this adaption, the notch filter could be adjusted to match the leakage/blocker offset frequency. Thus, digitally discrete programmability and analog continuous tuning capability are combined in this solution for improving the overall front-end interference robustness without de-sensitizing the Rx. Furthermore, the order of LPF/notch hybrid is programmable from 2 to 10 with a step of 2 for different rejection levels of interferences. The measured chip achieves a -3dB bandwidth of 6 21 MHz with 4-bit digital control and 1 MHz/step programmability, and a voltage gain of 0 70 dB with 9-bit digital control (3-bit from pre-amplifier, and 6-bit from PGA with 1 dB/step). With the condition of 15 dB gain, output P-1dB is 11.8 dBm@3MHz, and the output IP3 is 20.8 dBm@3MHz.

This paper presents a novel co-optimization configuration to simultaneously improve phase noise and FoM in order to solve the long-existing issue of excellent phase noise and mediocre FoM in the light of GaAs technologies. Considering traditional GaAs based Colpitts/class-C VCOs, a first step is taken with the introduction of noise shifting structure while a second step is carried out with the presentation of Darlington pair. Afterwards, a detailed outline of pros and cons of five VCO topologies, including Colpitts/class-C/noise shifting (NS) Colpitts/noise shifting class-C/noise shifting Darlington-based (NSDB) class-C VCO, is given in theory and in practice while respective start-up conditions and capacitive feedback factors are derived mathematically. It can be found that iterative phase noise and FoM improvement is achieved in GaAs based VCOs above in a gradual manner. Furthermore, the output power is stabilized with a simple trick of two-stage Darlington-based topology and thus, the power-hungry buffer is avoided. Ultimately, three VCOs out of the five are fabricated in GaAs technologies while their specifications are thoroughly compared with previous research. The fabricated Colpitts VCO demonstrates a frequency tuning range of 3.59–3.69 GHz with a current consumption of 11.2 mA@5 V supply, a phase noise of − 130.1 to − 129.1 dBc/Hz at 1 MHz offset from the carrier and the output power is around − 5 to − 5.1 dBm. The fabricated noise shifting Colpitts VCO demonstrates a frequency tuning range of 3.58–3.67 GHz with a current consumption of 10.8 mA@5 V supply, a phase noise of − 132.4 to − 130.2 dBc/Hz at 1 MHz offset from the carrier and the output power is around − 2.5 to − 3.5 dBm. The fabricated Darlington-based class-C VCO demonstrates a frequency tuning range of 2.76–2.91 GHz with a current consumption of 9.5 mA@5 V supply, a phase noise of − 138.6 to − 135.9 dBc/Hz at 1 MHz offset from the carrier and the output power is around − 3.06 to − 4.18 dBm. To the best of the authors’ expertise, the proposed Darlington-based class-C VCO achieves both exceptional phase noise and FoM, which far outweigh that of existing GaAs ones.

Nanoparticles (NPs) will inevitably interact with proteins and form protein coronas once they are exposed to biological fluids. This conventional model for nano-bio interactions has been used for over twenty years. Growing numbers of new nanomaterials are emerging every year. Among them, noble metal nanoclusters (NMNCs) are new types of fluorescent nanomaterials with considerable advantages in biomedical applications. Compared with NPs (typically >10 nm) like Au NPs, carbon nanotubes, etc., NMNCs have ultrasmall sizes (∼2 nm), so when NMNCs are exposed to biological milieu, will they form protein coronas like NPs? Due to a lack of characterization techniques for ultrasmall nanoparticles (USNPs), to date, studies on the binding stoichiometries of USNPs to proteins have been heavily hampered. To address this challenge, we combined the characteristics of various methods and selected human serum albumin (HSA) and transferrin (Trf) as model proteins to study their interactions with dihydrolipoic acid (DHLA) protected gold nanoclusters (DHLA-AuNCs). Steady-state fluorescence, transient fluorescence spectroscopy and isothermal titration calorimetry (ITC) were used to study the thermodynamic parameters (K, ΔH, ΔS, ΔG) and interaction mechanisms. The results showed that the intrinsic fluorescence of both proteins was quenched by DHLA-AuNCs, and the quenching process of HSA was an endothermic dynamic process. In contrast, the quenching process of Trf was an exothermic static process. The combination of ITC, agarose gel electrophoresis (AGE) and zeta potential showed that one HSA could bind 8 ± 1 DHLA-AuNCs and one Trf could bind 7 ± 2 DHLA-AuNCs, which was quite different from the conventional model of protein coronas. Based on these findings, the "protein complex" was termed for proteins upon binding with USNPs. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and atomic force microscopy (AFM) showed that DHLA-AuNCs could induce the agglomeration of proteins. Circular dichroism (CD) and synchronous fluorescence spectroscopy showed that DHLA-AuNCs had a very minor effect on the secondary structures of HSA and Trf, which demonstrated the good biocompatibility of DHLA-AuNCs at the molecular scale. This work has shed light on a new interaction model beyond the protein corona, indicating a possible biological identity of USNPs.

As-cast AlCrFeNi2Ti0.5 high entropy alloy was annealed at 600 °C and 800 °C for 6 h, 12 h and 24 h, respectively. The effect of annealing time on the microstructure and mechanical properties were investigated intensively. The as-cast sample showed a fine and homogeneous microstructure, and owned an excellent ductility of 16.3%. While the 600 °C, 12 h annealed sample showed the highest compressive strength of 2658 MPa and yield strength of 2046 MPa. The mechanical properties of the samples after 800 °C annealed for 12 h and 24 h deteriorated obviously. High annealing temperature promotes the growth of the crystal grains, and the new generated FeCr-type intermetallic phase leads to the embrittlement of the alloy.

Tetranychus cinnabarinus(Acari: Tetranychidae) is a worldwide polyphagous agricultural pest that has the title of resistance champion among arthropods. We reported previously the identification of the acaricidal compoundβ-sitosterol fromMentha piperitaandInula japonica. However, the acaricidal mechanism ofβ-sitosterol is unclear. Due to the limited genetic research carried out, wede novoassembled the transcriptome ofT. cinnabarinususing Illumina sequencing and conducted a differential expression analysis of control andβ-sitosterol-treated mites. In total, we obtained >5.4 G high-quality bases for each sample with unprecedented sequencing depth and assembled them into 22,941 unigenes. We identified 617 xenobiotic metabolism-related genes involved in detoxification, binding, and transporting of xenobiotics. A highly expanded xenobiotic metabolic system was found in mites.T. cinnabarinusdetoxification genes—including carboxyl/cholinesterase and ABC transporter class C—were upregulated afterβ-sitosterol treatment. Defense-related proteins, such as Toll-like receptor, legumain, and serine proteases, were also activated. Furthermore, other important genes—such as the chloride channel protein, cytochromeb, carboxypeptidase, peritrophic membrane chitin binding protein, and calphostin—may also play important roles in mites’ response toβ-sitosterol. Our results demonstrate that high-throughput-omics tool facilitates identification of xenobiotic metabolism-related genes and illustration of the acaricidal mechanisms ofβ-sitosterol.

XML structural index, which acts as a schema, plays an important role in XML query optimization and formulation. To provide a reasonable structural index for branching path query under space constraint, we propose an adaptive index of multiple local branching depths and multiple local bisimilarities, which is constructed by maximizing marginal gain for given query load. It cannot only give good support to branching path queries but also have much smaller size compared with that of same sort of index. Detailed experiments have shown that the index is effective and efficient for XML branching path query.