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Imaging in the second near-infrared window has attracted attention due to superior penetration depth and low signal interference. Here, the authors describe a new organic nano fluorophore with high quantum yield and demonstrate its use for in vivo imaging.

Background: Pain plays a significant role in emergency department (ED) visits, however safe and effective nonpharmacologic options are needed. Prior studies of acupuncture in the ED reported pain reduction with minimal side effects, but most were small and single site. Methods: We conducted ACUITY, a prospectively designed multi-center feasibility RCT. Our goal was to recruit 165 adults with acute non-emergent pain ≥4 on a 0–10-point scale at three EDs affiliated with BraveNet Practice Based Research Network. At baseline and 45–60 min later (post), participants self-assessed their pain and anxiety using a 0–10 rating scale. The primary feasibility outcome was recruitment of participants, whereas secondary outcomes were retention, and participant/provider acceptability. Results: From May 3, 2021, to September 24, 2022, 632 eligible individuals were approached and 165 enrolled (165/632: 26.1 %), meeting our recruitment goal. Notably, 42.4 % of enrollees were Black/African American, 42.4 % were White/Caucasian, and 13.9 % were Hispanic/Latino. Participants were randomized to Acupuncture (n = 83) or Usual care (n = 82), of which 151 (91.5 %) and 128 (77.6 %) provided pain and anxiety scores at post-treatment and 1-week respectively. Acupuncture was rated acceptable to participants and providers. Mean pain ratings (pre-to-post) were 7.4 (2.2) to 4.8 (2.8) for acupuncture and 7.1 (2.3) to 6.4 (2.5) for usual care. Mean anxiety ratings (pre-to-post) were 4.5 (3.4) to 2.5 (2.6) for acupuncture and 4.1 (3.4) to 3.5 (3.2) for usual care. Conclusion: Successful completion of ACUITY indicates we have the expertise and preliminary data to conduct a future definitive, multi-center RCT. Trial registration: Clinical trials.gov: NCT04880733.

Abstract The studies on the forest sector focus on energy issues and environmental challenges, but they are limited to a small number of studies focused on economic growth and social welfare. In the forest sector, Brazil is among the five countries with large forest cover in the world, with favorable conditions and great potential for production growth. Therefore, this work aimed to measure the evolution of efficiency and productivity of the 49 Brazilian forestry microregions in converting the expansion of economic growth into social welfare from 2009 to 2015 (a period of sectoral growth in the country). The approach of the Slack-Based Measure (SBM) – Data Envelopment Analysis (DEA), Malmquist Productivity Index (MPI), and Windows Analysis model was combined, followed by a solution for infeasibility problems. The results show that the growth of the forestry sector was not accompanied by the Human Development Index (HDI) in most of the microregions, showing regional and state differences, with the microregions close to the sensitive environmental areas with the lowest HDI. Thus, the work contributes to the design of public policies and government decision-making to increase the sector's efficiency and productivity and to social indicators that can guide sustainable policies in other contexts and countries.

The structure and chemistry of the dusty interstellar medium (ISM) are shaped by complex processes that depend on the local radiation field, gas composition, and dust grain properties. Of particular importance are polycyclic aromatic hydrocarbons (PAHs), which emit strong vibrational bands in the mid-infrared, and play a key role in the ISM energy balance. We recently identified global correlations between PAH band and optical line ratios across three nearby galaxies, suggesting a connection between PAH heating and gas ionization throughout the ISM. In this work, we perform a census of the PAH heating–gas ionization connection using ∼700,000 independent pixels that probe scales of 40–150 pc in 19 nearby star-forming galaxies from the PHANGS survey. We find a universal relation between $\mathrm{log}$ PAH(11.3 μ m/7.7 μ m) and $\mathrm{log}$ ([S ii ]/H α ) with a slope of ∼0.2 and a scatter of ∼0.025 dex. The only exception is a group of anomalous pixels that show unusually high (11.3 μ m/7.7 μ m) PAH ratios in regions with old stellar populations and high starlight-to-dust emission ratios. Their mid-infrared spectra resemble those of elliptical galaxies. Active galactic nucleus hosts show modestly steeper slopes, with a ∼​​​​​​10% increase in PAH(11.3 μ m/7.7 μ m) in the diffuse gas on kiloparsec scales. This universal relation implies an emerging simplicity in the complex ISM, with a sequence that is driven by a single varying property: the spectral shape of the interstellar radiation field. This suggests that other properties, such as gas-phase abundances, gas ionization parameter, and grain charge distribution, are relatively uniform in all but specific cases.

The L’Ralph instrument is a key component of NASA’s Lucy mission, intended to provide spectral image data of multiple Jupiter Trojans. The instrument operates from ∼0.35 to 4 μ m using two focal plane assemblies: a 350–950 nm multispectral imager, Multi-spectral Visible Imaging Camera (MVIC), and a 0.97–4 μ m imaging spectrometer, Linear Etalon Imaging Spectral Array (LEISA). Instrument calibration was established through ground testing before launch and has been monitored during cruise utilizing internal calibration sources and stellar targets. In-flight data have shown that the instrument thermal performance is exceeding expectations, allowing for early updates to LEISA radiometric and pointing calibrations. MVIC radiometric performance remains stable more than 3 yr since launch. The serendipitous identification of a new flyby target, (152830) Dinkinesh, allowed testing of instrument performance and interleaved LEISA and MVIC acquisitions on an asteroid target. Both MVIC and LEISA obtained data of Dinkinesh and its moon, Selam, demonstrating that they show good spectral agreement with an S- or Sq-type asteroid, along with evidence of a 3 μ m absorption feature.

Introducción: el desarrollo de actividades académicas a través de espacios virtuales contribuye a incrementar el uso de Smartphones en los universitarios, surgiendo la necesidad de estudiar los efectos de su uso. Objetivo: evaluar el efecto mediador de la distracción por Smartphone entre el tecnoestrés y la adicción a los Smartphone en universitarios de Lima Metropolitana. Método: Una muestra de 550 universitarios, con edades entre 18 y 35 años, fue evaluada con la Escala de distracción por Smartphone, la Escala de tecnoestrés para universitarios y la Escala de adicción basada en aplicaciones para teléfonos inteligentes. Se realizó un análisis de regresión para evaluar el rol mediador de la distracción por smartphones en la relación explicativa entre el tecnoestrés y la adicción a los celulares. Resultados: se identificó un efecto completo por parte del mediador, además se observa que existe un efecto indirecto del tecnoestrés sobre la adicción al smartphone. Discusión: la distracción por smartphone tiene un efecto sobre la adicción a los teléfonos celulares siempre y cuando esté presente la distracción como mediador.

Background: Previous studies have shown isokinetic exercise forms an important part in reconditioning the patients after anterior cruciate ligament reconstruction (ACLR) in regaining muscle strength and knee function. Although eccentric isokinetic training has been shown to enhance quadriceps muscle strength, the application toward benefiting patients after ACLR remains controversial. The present study aims to investigate the benefits of eccentric over concentric isokinetic exercises on knee muscle strength and its value in later stage of rehabilitation, including the return-to-sport. Methods: Thirty-six patients who had undergone ACLR for 4-to-6 months were assigned to receive either eccentric or concentric isokinetic training weekly for six weeks on top of their standardized post-operative exercise programme. The assessments include isokinetic test on the peak torques of quadriceps and hamstrings, single-leg hop test and ability to return-to-sport. Results: Both groups demonstrated significant gains on peak torques in quadriceps and hamstrings after training. At post-intervention, the peak torques for both quadriceps (p = 0.005) and hamstrings (p = 0.017) of the ACL-reconstructed limb from eccentric training were significantly higher than concentric training. The significant improvement was similarly demonstrated in the limb symmetry index (LSI) in hamstrings (p = 0.016) of the ACL-reconstructed limb from eccentric training. Moreover, eccentric group performed significantly better in single-leg hop tests (p = 0.042). Most importantly, eccentric group have higher percentages of return-to-sport (55.6 %) than concentric group (27.8 %). Conclusion: A 6-week course of eccentric isokinetic training was more effective than concentric isokinetic training in increasing quadriceps and hamstrings strength in terms of peak torques. Importantly, the better functional performance after the eccentric isokinetic exercise account for higher return-to-sport ratio.

Beginning in 2022, Nuevo Leon, Mexico, experienced an outbreak of rickettsioses that is still ongoing despite multidisciplinary control efforts. A total of 57 cases have been confirmed, particularly affecting children. We report a high mortality rate among hospitalized persons in Nuevo Leon. Continuing efforts are required to control the outbreak.

Abstract Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved in tissue reorganization. However, in contrast to genomic analysis, the actual biomolecular composition of the sample has fallen behind, leaving a gap of potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable of filling this gap. In this study we demonstrate spatially resolved Raman “spectromics” to reveal homogeneity, heterogeneity and dynamics of cell matrix on molecular levels by repurposing state-of-the-art bioinformatic analysis tools commonly used for transcriptomic analyses. By exploring sections of murine myocardial infarction and cardiac hypertrophy, we identify myocardial subclusters when spatially approaching the pathology, and define the surrounding metabolic and cellular (immune-) landscape. Our innovative, label-free, non-invasive “spectromics” approach could therefore open perspectives for a profound characterization of histological samples, while additionally allowing the combination with consecutive downstream analyses of the very same specimen.

[This corrects the article DOI: 10.1371/journal.pone.0265879.].

Em agosto de 2020 foi sancionada a Lei Geral de Proteção de Dados (LGPD), representando o marco regulatório no âmbito da proteção de dados. Em decorrência do que a lei dispõe sobre a responsabilidade civil, foram elaboradas diferentes correntes doutrinárias a respeito do tema, surgindo debates controversos com a finalidade de responder à seguinte indagação: se o regime de responsabilidade civil adotado pela LGPD é objetivo ou subjetivo. Sendo assim, esse estudo possui a finalidade de examinar o sistema de responsabilidade civil aplicado aos agentes de tratamento na Lei Geral de Proteção de Dados (Lei 13.709/18), apresentando uma análise crítica sobre os dispositivos que regem o tema responsabilidade civil, à luz dos diferentes pontos de vista dos doutrinadores brasileiros sobre os regimes de responsabilidade civil e sua possível aplicabilidade na Lei Geral de Proteção de Dados.

Background: Dilated cardiomyopathy with ataxia (DCMA) is an autosomal recessive disorder arising from truncating mutations in DNAJC19, which encodes an inner mitochondrial membrane protein. Clinical features include an early onset, often life-threatening, cardiomyopathy associated with other metabolic features. Here, we aim to understand the metabolic and pathophysiological mechanisms of mutant DNAJC19 for the development of cardiomyopathy. Methods: We generated induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) of two affected siblings with DCMA and a gene-edited truncation variant (tv) of DNAJC19 which all lack the conserved DnaJ interaction domain. The mutant iPSC-CMs and their respective control cells were subjected to various analyses, including assessments of morphology, metabolic function, and physiological consequences such as Ca2+ kinetics, contractility, and arrhythmic potential. Validation of respiration analysis was done in a gene-edited HeLa cell line (DNAJC19tvHeLa). Results: Structural analyses revealed mitochondrial fragmentation and abnormal cristae formation associated with an overall reduced mitochondrial protein expression in mutant iPSC-CMs. Morphological alterations were associated with higher oxygen consumption rates (OCRs) in all three mutant iPSC-CMs, indicating higher electron transport chain activity to meet cellular ATP demands. Additionally, increased extracellular acidification rates suggested an increase in overall metabolic flux, while radioactive tracer uptake studies revealed decreased fatty acid uptake and utilization of glucose. Mutant iPSC-CMs also showed increased reactive oxygen species (ROS) and an elevated mitochondrial membrane potential. Increased mitochondrial respiration with pyruvate and malate as substrates was observed in mutant DNAJC19tv HeLa cells in addition to an upregulation of respiratory chain complexes, while cellular ATP-levels remain the same. Moreover, mitochondrial alterations were associated with increased beating frequencies, elevated diastolic Ca2+ concentrations, reduced sarcomere shortening and an increased beat-to-beat rate variability in mutant cell lines in response to β-adrenergic stimulation. Conclusions: Loss of the DnaJ domain disturbs cardiac mitochondrial structure with abnormal cristae formation and leads to mitochondrial dysfunction, suggesting that DNAJC19 plays an essential role in mitochondrial morphogenesis and biogenesis. Moreover, increased mitochondrial respiration, altered substrate utilization, increased ROS production and abnormal Ca2+ kinetics provide insights into the pathogenesis of DCMA-related cardiomyopathy.

Background: Healthy aging and well-being are largely influenced by nutrition. Objective: The objective of the study was to evaluate the risk of malnutrition in elderly women residing in institutional care and its contributing factors. Methodology: One hundred institutionalized women aged 60 years and above were screened for malnutrition using Mini Nutritional Assessment (MNA) tool. Details on sociodemographic profile, physical activity, medical conditions, and food habits were gathered through researcher administered survey method. Results: The study participants had an average height of 149.70 cm (±7.31), weight of 50.72 kg (±9.11), body mass index of 22.77 kg/m2 (±4.68), body fat percentage of 31.30% (±8.99), mid-arm circumference of 27.36 cm (±7.84), calf circumference of 30.11 cm (±7.51), MNA score of 10.42 (±4.06), and hand grip strength score of 18.69 kg/lbs (±3.80). Upon analyzing the MNA scores, it was found that 9% of elderly women were well nourished, 62% were at risk of malnutrition, and 29% were malnourished. A significant difference was observed in the mean MNA scores (P < 0.05). Age, education level, body fat percentage, appetite, and dental problems were significantly associated with malnutrition (P < 0.05). Conclusion: Geriatric residents in old-age homes require adequate nutrition to maintain health. This can be achieved by providing individualized meal planning, reducing barriers to eating, and incorporating nutrient-dense foods.

Objectives To provide an integrated analysis of major adverse cardiovascular events (MACEs) and events of venous thromboembolism (VTE) and associated risk factors across rheumatoid arthritis (RA), psoriatic arthritis (PsA), and ankylosing spondylitis (AS) phase 2b/3 upadacitinib clinical programmes.Methods Data were analysed and summarised from clinical trials of RA, PsA and AS treated with upadacitinib 15 mg once daily (QD) and 30 mg QD (as of 30 June 2021). Data from adalimumab (RA and PsA) and methotrexate (RA) arms were included as comparators. Adjudicated MACEs and VTE events were presented as exposure-adjusted rates per 100 patient-years (E/100 PY). Univariable Cox proportional hazard regression analyses assessed potential associations of risk factors for MACE and VTE.Results In total, 4298 patients received upadacitinib 15 mg (RA n=3209, PsA n=907 and AS n=182) and 2125 patients received upadacitinib 30 mg (RA n=1204 and PsA n=921). In patients with RA and PsA, rates of MACE (0.3–0.6 E/100 PY) and VTE (0.2–0.4 E/100 PY) were similar across upadacitinib doses; in patients with AS, no MACEs and one VTE event occurred. Most patients experiencing MACEs or VTE events had two or more baseline cardiovascular risk factors. Across RA and PsA groups, rates of MACEs and VTE events were similar.Conclusions Rates of MACEs and VTE events with upadacitinib were consistent with previously reported data for patients receiving conventional synthetic and biologic disease-modifying anti-rheumatic drugs and comparable with active comparators adalimumab and methotrexate. Associated patient characteristics are known risk factors for MACEs and VTE events.Trial registration numbers RA (SELECT-NEXT: NCT02675426; SELECT-MONOTHERAPY: NCT02706951; SELECT-BEYOND: NCT02706847; SELECT-COMPARE: NCT02629159; SELECT-EARLY: NCT02706873, SELECT-CHOICE: NCT03086343), PsA (SELECT-PsA 2: NCT03104374; SELECT-PsA 1: NCT03104400), and AS (SELECT-AXIS 1: NCT03178487).

Background The increase in the number of autistic children being identified has led to increased demand on public schools to provide high-quality services. Effectively scaling up evidence-based practice (EBP) use for autistic students is challenging, given the complicated organization of special education. Teachers have significant challenges implementing autism EBP with fidelity. Factors such as implementation leadership and climate and attitudes toward EBP are linked to successful EBP use and may vary at different levels of the education system. Examining mechanisms of successful implementation is a critical step to support scale-up. Method In this observational study, conducted from September 2018 to March 2020, California school personnel ( n = 2273) at multiple levels of the system completed surveys related to implementation climate, leadership, and attitudes toward EBP. Data were collected throughout California at the Special Education Local Plan Areas, County Office of Education, and district and school levels from educators and administrators working in public schools supporting autistic students. Multi-level modeling was conducted to characterize implementation readiness. Results Overall, implementation climate and leadership scores are low across levels with regional levels rated more positively than districts or schools. Attitudes toward EBP were moderate, with those working in schools having the poorest ratings and specialists/trainers and related service providers (e.g., speech-language pathologists) having the highest ratings. Conclusions Outcomes provide a unique opportunity to compare implementation factors across organizational levels with a large, statewide sample. These data provide guidance for developing implementation interventions at multiple levels of the education system to increase readiness for effective scale-up of autism EBP in schools. Personnel and leaders at different organizational levels may need differentiated training targeting improved implementation climate and leadership. Personnel within districts and schools may experience a particular benefit from leadership support for EBP implementation.

Die Verknüpfung der Themen Medienbildung, Digitalisierung und Inklusion sowie damit einhergehender didaktische Mehrwerte und Herausforderungen für die Lehrpersonenbildung und das schulische Lernen sind Gegenstand des Beitrags. Im Rahmen des Lehrentwicklungsprojekts «Bildung im Kontext digitaler Medien unter Berücksichtigung von Inklusion» (BIDI) an der Leuphana Universität Lüneburg wurden medienpädagogische Inhalte und Ziele zu inklusiver Medienbildung im bildungswissenschaftlichen Teilstudium im B. A. Lehren und Lernen (Lehramt Grund-/Haupt-/Realschule) ausgeschärft und in ein Modul implementiert. Daran anknüpfend wurden in einem dialogischen Prozess mit ausgewählten Fachdidaktiken inhaltliche Verknüpfungen ausgelotet und bspw. im Rahmen des Profilstudiums «Digitales Lehren und Lernen» im Studiengang strukturell verankert. Flankierend wurde eine mehrperspektivische explorative qualitative Studie durchgeführt, bestehend aus Gruppendiskussionen mit Lehramtsstudierenden, die das Teilmodul Medienbildung absolviert haben, sowie Lehrenden aus den Fachdidaktiken. Das Erkenntnisinteresse der Studie fokussiert die Sichtweisen der Befragten auf die universitäre Lehre sowie auf Chancen und Herausforderungen im Zusammenspiel von Digitalisierung und Inklusion in der Lehrpersonenbildung und in der Schulpraxis. Die Befragten legten den Fokus auf Aspekte der Teilhabe im Zuge von Digitalisierung (u. a. hinsichtlich Medienausstattung, Lernzugängen, medialen Repräsentationen). Weiterhin wurde die Notwendigkeit einer kritisch-reflexiven Auseinandersetzung mit digitalen Medien und die Veränderung von fachlichen Lerngegenständen und Methoden im Zuge der Digitalisierung diskutiert.

Achieving gender equality in agricultural development is fundamental to reductions in global poverty, hunger, and malnutrition. African women make important contributions to farming and food systems; however, their efforts are often hindered by inefficient and inequitable allocations of intrahousehold labor and time that render women time poor. Time poverty is a root cause of women’s marginalization in rural Africa and an important area of inquiry for feminist scholarship. While gendered time use and time poverty have been researched in many different contexts and countries in Africa, significant knowledge gaps remain. Most studies consider women’s time use divorced from gendered relations, and overlook children’s contributions. Other factors which may combine to influence women’s time burden but are often overlooked include seasonality, work intensity, household structure and composition, cultural norms, familial relationships and intrahousehold power dynamics. Further, the majority of research on gendered time use and time poverty in Africa uses quantitative methods applied to secondary data, which presents challenges for critically identifying and characterizing the confluence of various intrahousehold dynamics which impact women’s multiple roles, responsibilities, and consequently their work and time. This study adds important nuance to the existing body of research by offering an in-depth, qualitative assessment of intrahousehold labor allocation, time use, and time poverty amongst women, men, and children living in multi-generational, largely polygamous households reliant on peanut-farming in the Kaolack region of Senegal. Data collection took place in February 2020, with 111 individuals in three villages. We find that individual workload correlates with gender and age, but is further determined by the demographic composition of the household, the roles assumed by the individual and other family members, and the individual’s place within the social hierarchy. Women and girls in Kaolack are clearly at more risk of time poverty due to their dual responsibility for reproductive and productive work, especially during the rainy season. Furthermore, women’s workload in particular changes over the life course as they assume different roles in different life stages. As a result, women with older daughters and, especially, daughters-in-law are significantly less time poor than other women.

Objective: To determine the incidence, outcomes, and evaluate diagnostic modalities for postoperative vocal cord dysfunction (VCD) following cardiothoracic surgery in children. Methods: A prospective mixed-methods study using principles of implementation science was completed. All patients undergoing surgery involving the aortic arch, ductus, or ligamentum arteriosum and vascular rings from September 2019 to December 2020 were enrolled. Patients underwent speech pathology assessment, laryngeal ultrasound, and flexible direct laryngoscopy. Results: Ninety-five patients were eligible for inclusion. The incidence of VCD ranged from 18% to 56% and varied according to procedure group. VCD occurred in 42% of neonates. Repair of hypoplastic aortic arch was associated with increased risk of VCD (57%; P = .002). There was no significant difference in duration of intubation, pediatric intensive care unit stay, or hospital stay. Forty percent children were able to achieve full oral feeding. Children with VCD were more likely to require nasogastric supplementary feeding at discharge (60% vs 36%; P = .044). Sixty-eight percent of patients demonstrated complete resolution of VCD at a median of 97 days postoperatively. Laryngeal ultrasound and speech pathology assessment combined had a sensitivity of 91% in comparison to flexible direct laryngoscopy. Conclusions: VCD occurred in one-third and resolved in two-thirds of patients at a median of 3 months following cardiac surgery. Aortic arch repair carried the highest risk of VCD. VCD adversely influenced feeding. Forty percent of patients achieved full oral feeding before discharge. VCD did not delay intensive care unit or hospital discharge. Speech pathology assessment and laryngeal ultrasound combined was reliable for diagnosis in most patients and was more patient friendly than flexible direct laryngoscopy.

We present resolved 3.6–250 μ m dust spectral energy distribution (SED) fitting for ∼800 nearby galaxies. We measure the distribution of radiation field intensities heating the dust, the dust mass surface density (Σ _d ), and the fraction of dust in the form of polycyclic aromatic hydrocarbons (PAHs; q _PAH ). We find that the average interstellar radiation field ( $\overline{U}$ ) is correlated both with stellar mass surface density (Σ _⋆ ) and star formation rate surface density (Σ _SFR ), while more intense radiation fields are only correlated with Σ _SFR . We show that q _PAH is a steeply decreasing function of Σ _SFR , likely reflecting PAH destruction in H ii regions. Galaxy-integrated q _PAH is strongly, negatively correlated with specific star formation rate (sSFR) and offset from the star-forming “main sequence” (ΔMS), suggesting that both metallicity and star formation intensity play a role in setting the global q _PAH . We also find a nearly constant M _d / M _* ratio for galaxies on the main sequence, with a lower ratio for more quiescent galaxies, likely due to their lower gas fractions. From these results, we construct prescriptions to estimate the radiation field distribution in both integrated and resolved galaxies. We test these prescriptions by comparing our predicted $\overline{U}$ to results of SED fitting for stacked “main-sequence” galaxies at 0 < z < 4 from M. Béthermin et al. and find sSFR is an accurate predictor of $\overline{U}$ even at these high redshifts. Finally, we describe the public delivery of matched-resolution Wide-field Infrared Survey Explorer and Herschel maps along with the resolved dust SED-fitting results through the Infrared Science Archive.

The PHANGS survey uses Atacama Large Millimeter/submillimeter Array, Hubble Space Telescope, Very Large Telescope, and JWST to obtain an unprecedented high-resolution view of nearby galaxies, covering millions of spatially independent regions. The high dimensionality of such a diverse multiwavelength data set makes it challenging to identify new trends, particularly when they connect observables from different wavelengths. Here, we use unsupervised machine-learning algorithms to mine this information-rich data set to identify novel patterns. We focus on three of the PHANGS-JWST galaxies, for which we extract properties pertaining to their stellar populations; warm ionized and cold molecular gas; and polycyclic aromatic hydrocarbons (PAHs), as measured over 150 pc scale regions. We show that we can divide the regions into groups with distinct multiphase gas and PAH properties. In the process, we identify previously unknown galaxy-wide correlations between PAH band and optical line ratios and use our identified groups to interpret them. The correlations we measure can be naturally explained in a scenario where the PAHs and the ionized gas are exposed to different parts of the same radiation field that varies spatially across the galaxies. This scenario has several implications for nearby galaxies: (i) The uniform PAH ionized fraction on 150 pc scales suggests significant self-regulation in the interstellar medium, (ii) the PAH 11.3/7.7 μ m band ratio may be used to constrain the shape of the non-ionizing far-ultraviolet to optical part of the radiation field, and (iii) the varying radiation field affects line ratios that are commonly used as PAH size diagnostics. Neglecting this effect leads to incorrect or biased PAH sizes.

The exquisite angular resolution and sensitivity of JWST are opening a new window for our understanding of the Universe. In nearby galaxies, JWST observations are revolutionizing our understanding of the first phases of star formation and the dusty interstellar medium. Nineteen local galaxies spanning a range of properties and morphologies across the star-forming main sequence have been observed as part of the PHANGS-JWST Cycle 1 Treasury program at spatial scales of ∼5–50 pc. Here, we describe pjpipe , an image-processing pipeline developed for the PHANGS-JWST program that wraps around and extends the official JWST pipeline. We release this pipeline to the community as it contains a number of tools generally useful for JWST NIRCam and MIRI observations. Particularly for extended sources, pjpipe products provide significant improvements over mosaics from the MAST archive in terms of removing instrumental noise in NIRCam data, background flux matching, and calibration of relative and absolute astrometry. We show that slightly smoothing F2100W MIRI data to 0.″9 (degrading the resolution by about 30%) reduces the noise by a factor of ≈3. We also present the first public release (DR1.1.0) of the pjpipe processed eight-band 2–21 μ m imaging for all 19 galaxies in the PHANGS-JWST Cycle 1 Treasury program. An additional 55 galaxies will soon follow from a new PHANGS-JWST Cycle 2 Treasury program.

Polycyclic aromatic hydrocarbons (PAHs) are a ubiquitous component of the interstellar medium (ISM) in z ∼ 0 massive, star-forming galaxies and play key roles in ISM energy balance, chemistry, and shielding. Wide field-of-view, high-resolution mid-infrared (MIR) images from JWST provide the ability to map the fraction of dust in the form of PAHs and the properties of these key dust grains at 10–50 pc resolution in galaxies outside the Local Group. We use MIR JWST photometric observations of a sample of 19 nearby galaxies from the Physics at High Angular Resolution in Nearby GalaxieS (PHANGS) survey to investigate the variations of the PAH fraction. By comparison to lower-resolution far-infrared mapping, we show that a combination of the MIRI filters ( R _PAH = [F770W+F1130W]/F2100W) traces the fraction of dust by mass in the form of PAHs (i.e., the PAH fraction, or q _PAH ). Mapping R _PAH across the 19 PHANGS galaxies, we find that the PAH fraction steeply decreases in H ii regions, revealing the destruction of these small grains in regions of ionized gas. Outside H ii regions, we find R _PAH is constant across the PHANGS sample with an average value of 3.43 ± 0.98, which, for an illuminating radiation field of intensity 2–5 times that of the radiation field in the solar neighborhood, corresponds to q _PAH values of 3%–6%.

Morphological mapping is a fundamental step in studying the processes that shaped an asteroid surface. However, it is challenging and often requires multiple independent assessments by trained experts. Here we present fast methods to detect and characterize meaningful terrains from the topographic roughness: entropy of information, and local mean surface orientation. We apply our techniques to Didymos and Dimorphos, the target asteroids of NASA's Double Asteroid Redirection Test mission—the first attempt to deflect an asteroid. Our methods reliably identify morphological units at multiple scales. The comparative study reveals various terrain types, signatures of processes that transformed Didymos and Dimorphos. Didymos shows the most heterogeneity and morphology that indicate recent resurfacing events. Dimorphos is comparatively rougher than Didymos, which may result from the formation process of the binary pair and past interaction between the two bodies. Our methods can be readily applied to other bodies and data sets.

ACKR3 is a critical regulator of platelet-mediated thrombosis and organ injury following ischemia/reperfusion. Platelet ACKR3 surface expression is independently associated with all-cause mortality in patients with cardiovascular diseases.

Ex vivo expansion conditions used to generate T cells for immunotherapy are thought to adopt metabolic phenotypes that impede therapeutic efficacy in vivo. The comparison of five different culture media used for clinical T cell expansion revealed unique optima based on different output variables, including proliferation, differentiation, function, activation, and mitochondrial phenotypes. The extent of proliferation and function depended on the culture media rather than stimulation conditions. Moreover, the expanded T cell end products adapted their metabolism when switched to a different media formulation, as shown by glucose and glutamine uptake and patterns of glucose isotope labeling. However, adoption of these metabolic phenotypes was uncoupled to T cell function. Expanded T cell products cultured in ascites from ovarian cancer patients displayed suppressed mitochondrial activity and function irrespective of the ex vivo expansion media. Thus, ex vivo T cell expansion media have profound impacts on metabolism and function.

The glucocorticoid receptor (GR) is an important anti-cancer target in lymphoid cancers but has been understudied in solid tumors like lung cancer, although glucocorticoids are often given with chemotherapy regimens to mitigate side effects. Here, we identify a dexamethasone-GR mediated anti-cancer response in a subset of aggressive non-small cell lung cancers (NSCLCs) that harbor Serine/Threonine Kinase 11 (STK11/LKB1) mutations. High tumor expression of carbamoyl phosphate synthase 1 (CPS1) was strongly linked to the presence of LKB1 mutations, was the best predictor of NSCLC dexamethasone (DEX) sensitivity (p < 10-16) but was not mechanistically involved in DEX sensitivity. Subcutaneous, orthotopic and metastatic NSCLC xenografts, biomarker-selected, STK11/LKB1 mutant patient derived xenografts, and genetically engineered mouse models with KRAS/LKB1 mutant lung adenocarcinomas all showed marked in vivo anti-tumor responses with the glucocorticoid dexamethasone as a single agent or in combination with cisplatin. Mechanistically, GR activation triggers G1/S cell cycle arrest in LKB1 mutant NSCLCs by inducing the expression of the cyclin-dependent kinase inhibitor, CDKN1C/p57(Kip2). All findings were confirmed with functional genomic experiments including CRISPR knockouts and exogenous expression. Importantly, DEX-GR mediated cell cycle arrest did not interfere with NSCLC radiotherapy, or platinum response in vitro or with platinum response in vivo. While DEX induced LKB1 mutant NSCLCs in vitro exhibit markers of cellular senescence and demonstrate impaired migration, in vivo DEX treatment of a patient derived xenograft (PDX) STK11/LKB1 mutant model resulted in expression of apoptosis markers. These findings identify a previously unknown GR mediated therapeutic vulnerability in STK11/LKB1 mutant NSCLCs caused by induction of p57(Kip2) expression with both STK11 mutation and high expression of CPS1 as precision medicine biomarkers of this vulnerability.

BackgroundEndometriosis is a complex chronic disease characterized by pain, including painful sex, that can contribute to considerable sexual function, self-esteem, and relationship challenges. Digital storytelling is an arts-based, participatory methodology wherein individuals create and share their illness experiences in detailing their lived experiences. ObjectiveThe study objective was to pilot-test a web-based digital storytelling workshop focused on endometriosis to understand storytellers’ experiences of workshop participation. We assessed the feasibility of story cocreation and sharing, including the emotional impact of workshop participation, the acceptability of the workshop for the subject matter, and the storytellers’ willingness to share their stories with broader audiences as a method for knowledge translation. MethodsThis study used a community-based participatory methodology supplemented with patient-oriented research and integrated knowledge translation. Study participants, referred to as storytellers, cocreated 3- to 5-minute individual digital stories about their lived experiences of endometriosis during a web-based workshop (comprising five 2-hour sessions over 6 weeks) facilitated by The Center for Digital Storytelling. Data were collected through participant observations at the workshop, storyteller weekly reflective journals, and an end-of-workshop focus group interview with storytellers. These data were analyzed using a qualitative interpretive description approach. ResultsA total of 5 women and 1 nonbinary storyteller aged 19 to 39 years who had experienced endometriosis for 4 to 22 years participated in the study. We characterized storytelling workshop participation and the acceptability of story cocreation by describing participants’ experiences of opportunity, commitment, and connection; complex emotions that were healing; and a desire to share. Feasibility was demonstrated through 100% engagement in the workshops. All 6 storytellers reported feeling empowered by publicly sharing their cocreated digital stories through social media and the Sex, Pain & Endometriosis website. ConclusionsDespite the complexities of the story-building process, the workshop and the cocreation and sharing of digital stories were feasible. The storytellers found that this process allowed for emotional healing and personal empowerment by offering a unique way to talk about painful sex, which also facilitated a connection among those in the workshop. The use of digital storytelling as a knowledge translation tool shows promise, and this approach also has potential as a therapeutic intervention.

BackgroundAnxiety disorders are the most common mental disorders worldwide. In Hong Kong, 7% of adolescents are diagnosed with anxiety disorders, and 1 in every 4 secondary school students reports clinical-level anxiety symptoms. However, 65% of them do not access services. Long waitlists in public services, the high cost of private services, or the fear of being stigmatized can hinder service access. The high prevalence of anxiety and low intervention uptake indicate a pressing need to develop timely, scalable, and potent interventions suitable for adolescents. Single-session interventions (SSIs) have the potential to be scalable interventions for diagnosable or subclinical psychopathology in adolescents. Providing precise and context-adapted intervention is the key to achieving intervention efficacy. ObjectiveThis study aims to compare the effectiveness of three SSIs: single-session intervention of growth mindset on negative emotions (SIGMA), SSI of growth mindset of personality (SSI-GP), and active control, in reducing adolescent anxiety. MethodsAdolescents (N=549, ages 12-16 years) from secondary schools will be randomized to 1 of 3 intervention conditions: the SIGMA, SSI-GP, or active control. The implementation of each intervention is approximately 45 minutes in length. Adolescent participants will report anxiety symptoms (primary outcome), perceived control, hopelessness, attitude toward help-seeking, and psychological well-being at preintervention, the 2-week follow-up, and the 8-week follow-up. A pilot test has confirmed the feasibility and acceptability of SIGMA among adolescents. We hypothesized that SIGMA and SSI-GP will result in a larger reduction in anxiety symptoms than the control intervention during the posttest and 8-week follow-up period. We also predict that SIGMA will have a more significant effect than SSI-GP. We will use the intention-to-treat principle and linear regression-based maximum likelihood multilevel models for data analysis. ResultsThis study will be conducted from December 2022 to December 2023, with results expected to be available in January 2024. ConclusionsThis protocol introduces the implementation content and strategies of growth mindset SSIs (consists of 2 forms: SIGMA and SSI-GP) among school students. The study will provide evidence on the efficacy of different growth mindset SSIs for adolescent anxiety. It will also establish implementation strategies for self-administrative SSIs among school students, which can serve as a pioneer implementation of a scalable and self-accessible brief intervention to improve the well-being of young people. Trial RegistrationClinicalTrials.gov NCT05027880; https://clinicaltrials.gov/ct2/show/NCT05027880 International Registered Report Identifier (IRRID)PRR1-10.2196/41758

Abstract Background Food Protein-Induced Enterocolitis Syndrome (FPIES) is a non-IgE mediated food allergy most commonly presenting in infants. The most common food triggers include soy, cow’s milk and grains. Symptoms may include intractable vomiting, diarrhea, lethargy, pallor, abdominal distention, hypotension and/or shock. Oral food challenges (OFCs) given at food protein dose of 0.06–0.6 g/kg in 3 equivalent doses administered over a few hours are recommended in guidelines to confirm a diagnosis. Case presentation The patient is a 6-month-old girl with a history of severe FPIES symptoms to egg. In our clinic, we perform OFC with 1/100 serving dose on visit 1 and then increase the dose monthly. The patient takes the tolerated dose daily at home between visits. An OFC to baked egg at 1/100 of a serving was performed and was well-tolerated on her initial visit. The patient remained on the same dose upon returning home. Within 1-week, she developed FPIES symptoms including watery diarrhea and severe emesis requiring ondansetron. She required an Emergency Department visit for one of the reactions. Conclusions Our patient had severe FPIES symptoms with a small amount of egg. We believe that administration of three large food challenge doses on one clinic visit, as guidelines currently suggest, does not allow adequate time for symptoms to appear. Our patient likely would have suffered a severe reaction. Also, this guidelines protocol does not allow for monitoring of more delayed or chronic FPIES. We propose a modified protocol to OFCs with cautious up-dosing to allow for safer OFCs and monitoring of chronic FPIES. We have implemented an OFC approach where only one food challenge dose (starting with 1/100 of final dose) is given at each visit. The up-titration of the dose is completed every 4-weeks with one dose only. When the serving sized dose is reached and tolerated, the food can be maintained in the diet.

The reproductive neuroendocrine system is a key target for the developmental programming effects of steroid hormones during early life. While gonadal steroids play an important role in controlling the physiological development of the neuroendocrine axis, human fetuses are susceptible to adverse programming due to exposure to endocrine disrupting chemicals with steroidal activity, inadvertent use of contraceptive pills during pregnancy, as well as from disease states that result in abnormal steroid production. Animal models provide an unparalleled resource to understand the effects of steroid hormones on the development of the neuroendocrine axis and their role on the developmental origins of health and disease. In female sheep, exposure to testosterone (T) excess during fetal development results in an array of reproductive disorders that recapitulate those seen in women with polycystic ovary syndrome (PCOS), including disrupted neuroendocrine feedback mechanisms, increased pituitary responsiveness to gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH) hypersecretion, functional hyperandrogenism, multifollicular ovarian morphology, and premature reproductive failure. Similar to a large proportion of women with PCOS, these prenatally T-treated sheep also manifest insulin resistance and cardiovascular alterations, including hypertension. This review article focuses on the effects of prenatal androgens on the developmental programming of hypothalamic and pituitary alterations in the sheep model of PCOS phenotype, centering specifically on key neurons, neuropeptides, and regulatory pathways controlling GnRH and LH secretion. Insights obtained from the sheep model as well as other animal models of perinatal androgen excess can have important translational relevance to treat and prevent neuroendocrine dysfunction in women with PCOS and other fertility disorders.

The PHANGS collaboration has been building a reference data set for the multiscale, multiphase study of star formation and the interstellar medium (ISM) in nearby galaxies. With the successful launch and commissioning of JWST, we can now obtain high-resolution infrared imaging to probe the youngest stellar populations and dust emission on the scales of star clusters and molecular clouds (∼5–50 pc). In Cycle 1, PHANGS is conducting an eight-band imaging survey from 2 to 21 μ m of 19 nearby spiral galaxies. Optical integral field spectroscopy, CO(2–1) mapping, and UV-optical imaging for all 19 galaxies have been obtained through large programs with ALMA, VLT-MUSE, and Hubble. PHANGS–JWST enables a full inventory of star formation, accurate measurement of the mass and age of star clusters, identification of the youngest embedded stellar populations, and characterization of the physical state of small dust grains. When combined with Hubble catalogs of ∼10,000 star clusters, MUSE spectroscopic mapping of ∼20,000 H ii regions, and ∼12,000 ALMA-identified molecular clouds, it becomes possible to measure the timescales and efficiencies of the earliest phases of star formation and feedback, build an empirical model of the dependence of small dust grain properties on local ISM conditions, and test our understanding of how dust-reprocessed starlight traces star formation activity, all across a diversity of galactic environments. Here we describe the PHANGS–JWST Treasury survey, present the remarkable imaging obtained in the first few months of science operations, and provide context for the initial results presented in the first series of PHANGS–JWST publications.

We present maps tracing the fraction of dust in the form of polycyclic aromatic hydrocarbons (PAHs) in IC 5332, NGC 628, NGC 1365, and NGC 7496 from JWST/MIRI observations. We trace the PAH fraction by combining the F770W (7.7 μ m) and F1130W (11.3 μ m) filters to track ionized and neutral PAH emission, respectively, and comparing the PAH emission to F2100W, which traces small, hot dust grains. We find the average R _PAH = (F770W + F1130W)/F2100W values of 3.3, 4.7, 5.1, and 3.6 in IC 5332, NGC 628, NGC 1365, and NGC 7496, respectively. We find that H ii regions traced by MUSE H α show a systematically low PAH fraction. The PAH fraction remains relatively constant across other galactic environments, with slight variations. We use CO+H i +H α to trace the interstellar gas phase and find that the PAH fraction decreases above a value of ${{\rm{I}}}_{{\rm{H}}\alpha }/{{\rm{\Sigma }}}_{{\rm{H}}{\rm\small{I}}+{{\rm{H}}}_{2}}\sim {10}^{37.5}\,\mathrm{erg}\,{{\rm{s}}}^{-1}\,{\mathrm{kpc}}^{-2}\,{({M}_{\odot }\,{\mathrm{pc}}^{-2})}^{-1}$ in all four galaxies. Radial profiles also show a decreasing PAH fraction with increasing radius, correlated with lower metallicity, in line with previous results showing a strong metallicity dependence to the PAH fraction. Our results suggest that the process of PAH destruction in ionized gas operates similarly across the four targets.

JWST observations of polycyclic aromatic hydrocarbon (PAH) emission provide some of the deepest and highest resolution views of the cold interstellar medium (ISM) in nearby galaxies. If PAHs are well mixed with the atomic and molecular gas and illuminated by the average diffuse interstellar radiation field, PAH emission may provide an approximately linear, high-resolution, high-sensitivity tracer of diffuse gas surface density. We present a pilot study that explores using PAH emission in this way based on Mid-Infrared Instrument observations of IC 5332, NGC 628, NGC 1365, and NGC 7496 from the Physics at High Angular resolution in Nearby GalaxieS-JWST Treasury. Using scaling relationships calibrated in Leroy et al., scaled F1130W provides 10–40 pc resolution and 3 σ sensitivity of Σ _gas ∼ 2 M _⊙ pc ^−2 . We characterize the surface densities of structures seen at

We present a comparison of theoretical predictions of dust continuum and polycyclic aromatic hydrocarbon (PAH) emission with new JWST observations in three nearby galaxies: NGC 628, NGC 1365, and NGC 7496. Our analysis focuses on a total of 1063 compact stellar clusters and 2654 stellar associations previously characterized by the Hubble Space Telescope in the three galaxies. We find that the distributions and trends in the observed PAH-focused infrared colors generally agree with theoretical expectations, and that the bulk of the observations is more aligned with models of larger, ionized PAHs. These JWST data usher in a new era of probing interstellar dust and studying how the intense radiation fields near stellar clusters and associations play a role in shaping the physical properties of PAHs.

Polycyclic aromatic hydrocarbons (PAHs) play a critical role in the reprocessing of stellar radiation and balancing the heating and cooling processes in the interstellar medium but appear to be destroyed in H ii regions. However, the mechanisms driving their destruction are still not completely understood. Using PHANGS–JWST and PHANGS–MUSE observations, we investigate how the PAH fraction changes in about 1500 H ii regions across four nearby star-forming galaxies (NGC 628, NGC 1365, NGC 7496, and IC 5332). We find a strong anticorrelation between the PAH fraction and the ionization parameter (the ratio between the ionizing photon flux and the hydrogen density) of H ii regions. This relation becomes steeper for more luminous H ii regions. The metallicity of H ii regions has only a minor impact on these results in our galaxy sample. We find that the PAH fraction decreases with the H α equivalent width—a proxy for the age of the H ii regions—although this trend is much weaker than the one identified using the ionization parameter. Our results are consistent with a scenario where hydrogen-ionizing UV radiation is the dominant source of PAH destruction in star-forming regions.

Ratios of polycyclic aromatic hydrocarbon (PAH) vibrational bands are a promising tool for measuring the properties of the PAH population and their effect on star formation. The photometric bands of the MIRI and NIRCam instruments on JWST provide the opportunity to measure PAH emission features across entire galaxy disks at unprecedented resolution and sensitivity. Here we present the first results of this analysis in a sample of three nearby galaxies: NGC 628, NGC 1365, and NGC 7496. Based on the variations observed in the 3.3, 7.7, and 11.3 μ m features, we infer changes to the average PAH size and ionization state across the different galaxy environments. High values of F335M _PAH /F1130W and low values of F1130W/F770W are measured in H ii regions in all three galaxies. This suggests that these regions are populated by hotter PAHs, and/or that the PAH ionization fraction is larger. We see additional evidence of heating and/or changes in PAH size in regions with higher molecular gas content as well as increased ionization in regions with higher H α intensity.

We present maps of the 3.3 μ m polycyclic aromatic hydrocarbon (PAH) emission feature in NGC 628, NGC 1365, and NGC 7496 as observed with the Near-Infrared Camera imager on JWST from the PHANGS–JWST Cycle 1 Treasury project. We create maps that isolate the 3.3 μ m PAH feature in the F335M filter (F335M _PAH ) using combinations of the F300M and F360M filters for removal of starlight continuum. This continuum removal is complicated by contamination of the F360M by PAH emission and variations in the stellar spectral energy distribution slopes between 3.0 and 3.6 μ m. We modify the empirical prescription from Lai et al. to remove the starlight continuum in our highly resolved galaxies, which have a range of starlight- and PAH-dominated lines of sight. Analyzing radially binned profiles of the F335M _PAH emission, we find that between 5% and 65% of the F335M intensity comes from the 3.3 μ m feature within the inner 0.5 r _25 of our targets. This percentage systematically varies from galaxy to galaxy and shows radial trends within the galaxies related to each galaxy’s distribution of stellar mass, interstellar medium, and star formation. The 3.3 μ m emission is well correlated with the 11.3 μ m PAH feature traced with the MIRI F1130W filter, as is expected, since both features arise from C–H vibrational modes. The average F335M _PAH /F1130W ratio agrees with the predictions of recent models by Draine et al. for PAHs with size and charge distributions shifted toward larger grains with normal or higher ionization.

Este artigo discute a articulação entre comunicação, resistência e luta pela sobrevivência das comunidades quilombolas na pandemia da Covid-19. Traz a análise de conteúdo das publicações no Instagram e das entrevistas com integrantes da Coordenação Nacional de Articulação das Comunidades Negras Rurais Quilombolas (Conaq) e da Federação das Comunidades Quilombolas do Estado de Minas Gerais (N’Golo). Os resultados apontam várias desconexões (digital, estradas, eletricidade) que dificultam a comunicação. Neste cenário, o WhatsApp aparece como elo entre organizações e comunidades. Destacam-se as estratégias de aproximação e a identificação da figura dos mediadores (donos dos celulares) no repasse das informações à população nos territórios quilombolas.