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Aeromonas hydrophila currently has the status of a foodborne pathogen causing zoonotic diseases spreading from animals to humans. Sixty of typically hemorrhagic diseased freshwater fishes were collected from twelve aquafarms in Anhui Province. Twenty of A. hydrophila isolates were isolated and characterized by RAPD-PCR, antibiotics susceptibility testing and determination of virulence factors. RAPD-PCR fingerprinting revealed the complex diversity and genetic polymorphism (I-XIV RAPD types) with D of 0.958 on 90% patterns similarity and eight resistance patterns were observed by antibiotics susceptibility testing with D of 0.747. Furthermore, the virulence genes were present in 85% (aer), 40% (epr), 75% (ast), 35% (ahyB), 35% (act) and 80% (alt) of the strains, respectively. The result indicated that the same characterization (I RAPD type, resistance pattern and virulence factors) was found in A. hydrophila isolates from A aquafram, showing their close genetic relationship or origins. [ABSTRACT FROM AUTHOR]

We present a new geometric distortion model for the narrow-field mode of the near-infrared camera (NIRC2) fed by the adaptive optics system on the W. M. Keck II telescope. The adaptive optics system and NIRC2 camera were realigned on 2015 April 13. Observations of the crowded globular cluster, M53, were obtained before and after the realignment to characterize the geometric field distortion. The distorted NIRC2 positions of M53 stars were compared with precise astrometry of this cluster from Hubble Space Telescope observations. The resulting distortion map constructed just before the realignment is consistent with the previous solution derived using data from 2007 to 2009, indicating that the distortion has been stable to ∼0.5 mas. The distortion map changed significantly after a realignment of 4.5 mas (75%) rms, and the new distortion model for post-realignment observations have a total accuracy of ∼1.1 mas. [ABSTRACT FROM AUTHOR]