1. Isolation of monomeric photosystem II that retains the subunit PsbS
- Author
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Claudia Büchel, Matthias Bochtler, Wolfgang P. Schröder, Maria Cecilia Loi, Thomas Kieselbach, Patrycja Haniewicz, Dario Piano, Daniele de Sanctis, Int Inst Mol & Cell Biol, PL-02109 Warsaw, Poland, European Synchrotron Radiation Facility (ESRF), Goethe Univ Frankfurt, Inst Mol Biosci, D-60438 Frankfurt, Germany, Inst Chem, S-90187 Umea, Sweden, Umea Plant Science Center (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU)-Swedish University of Agricultural Sciences (SLU), Univ Cagliari, Dept Life & Environm Sci, I-09123 Cagliari, Italy, and Inst Biochem & Biophys, Dept Bioinformat, PL-02106 Warsaw, Poland
- Subjects
0106 biological sciences ,Chlorophyll ,Photosystem II ,Stereochemistry ,Nicotiana tabacum ,Protein subunit ,[SDV]Life Sciences [q-bio] ,Thylakoid membranes ,Light-Harvesting Protein Complexes ,Oligomeric state ,macromolecular substances ,Plant Science ,Biology ,Photosynthesis ,01 natural sciences ,Thylakoids ,Biochemistry ,Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,Tobacco ,Regular Paper ,Polyacrylamide gel electrophoresis ,030304 developmental biology ,Plant Proteins ,0303 health sciences ,PsbS ,Biochemistry and Molecular Biology ,Photosystem II Protein Complex ,food and beverages ,General Medicine ,Cell Biology ,biology.organism_classification ,Oxygen ,Protein Subunits ,Monomer ,chemistry ,Thylakoid ,Electrophoresis, Polyacrylamide Gel ,Biokemi och molekylärbiologi ,010606 plant biology & botany ,Transplastomic plant - Abstract
International audience; Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants
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