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Giardia duodenalis and Cryptosporidium spp. are common intestinal protozoa that can cause diarrhoeal disease. Although cases of infection with Giardia and Cryptosporidium have been reported in Alaska, the seroprevalence and correlates of exposure to these parasites have not been characterised. We conducted a seroprevalence survey among 887 residents of Alaska, including sport hunters, wildlife biologists, subsistence bird hunters and their families and non-exposed persons. We tested serum using a multiplex bead assay to evaluate antibodies to the Giardia duodenalis variant-specific surface protein conserved structural regions and to the Cryptosporidium parvum 17- and 27-kDa antigens. Approximately one third of participants in each group had evidence of exposure to Cryptosporidium. Prevalence of Giardia antibody was highest among subsistence hunters and their families (30%), among whom positivity was associated with lack of community access to in-home running water (adjusted prevalence ratio [aPR] 1.15, 95% confidence interval (CI) 1.02-1.28) or collecting rain, ice, or snow to use as drinking water (aPR 1.09, 95% CI 1.01-1.18). Improving in-home water access for entire communities could decrease the risk of exposure to Giardia. [ABSTRACT FROM AUTHOR]

Background. After the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7) in Alaska, the incidence of invasive pneumococcal disease (IPD) due to non-vaccine serotypes, particularly serotype 19A, increased. The aim of this study was to describe the molecular epidemiology of IPD due to serotype 19A in Alaska. Methods. IPD data were collected from 1986 to 2010 through population-based laboratory surveillance. Isolates were serotyped by the Quellung reaction and MICs determined by broth microdilution. Genotypes were assessed by multilocus sequence typing. Results. Among 3,294 cases of laboratory-confirmed IPD, 2,926 (89%) isolates were available for serotyping, of which 233 (8%) were serotype 19A. Across all ages, the proportion of IPD caused by serotype 19A increased from 3.5% (63/1823) pre-PCV7 (1986-2000) to 15.4% (170/1103) post-PCV7 (2001-2010) (p<0.001); among children B5 years of age, the proportion increased from 5.0% (39/776) to 33.0% (76/230) (p<0.001). The annual incidence rate of IPD due to serotype 19A (all ages) increased from 0.73 cases pre-PCV7 to 2.56 cases/ 100,000 persons post-PCV7 (p<0.001); rates among children <5 years of age increased from 4.84 cases to 14.1 cases/100,000 persons (p<0.001). Among all IPD isolates with reduced susceptibility to penicillin, 17.8% (32/180) were serotype 19A pre-PCV7 and 64% (121/189) were serotype 19A post-PCV7 (p<0.001). Eighteen different sequence types (STs) were identified; ST199 or single locus variants of ST199 (n=150) and ST172 (n=59) accounted for the majority of isolates. Multidrug-resistant isolates were clustered in ST199 and ST320. Conclusion. While PCV13 should significantly reduce the burden of disease due to 19A, these data highlight the need to continue surveillance for IPD to monitor the effects of vaccination on the expansion and emergence of non-PCV strains. [ABSTRACT FROM AUTHOR]

Presents a report summarizing the outbreak of invasive pneumococcal disease (IPD) in a rural region in Alaska during 2003-2004. Role of the Arctic Investigations Program in conducting laboratory-based surveillance for IPD in Alaska; Number of cases of IPD reported in the region from January 2003 to March 2004; Percentage of persons with Streptococcus pneumoniae in the region.

The purpose of this work is to determine ion temperature profiles in spheromaks [Nucl. Fusion 19, 489 (1979)] for the first time. Knowledge of the ion temperature profile is necessary in the correct calculation of plasma confinement parameters. The work herein details the calculation of ion temperature profiles for the Compact Torus Experiment (CTX) [Nucl. Fusion 28, 1555 (1988)] and S-1 [Phys. Rev. Lett. 46, 188 (1981)] spheromaks. Data from single chord Doppler ion temperature measurements in these devices have been analyzed with the aid of a one-dimensional equilibrium charge state transport code. Using electron temperature and density profiles from Thomson scattering, and estimates for transport rates, a most probable position for the emission of line radiation can be determined and correlated with the measured Doppler ion temperature, thus generating an ion temperature profile. From this ion temperature profile determination, plasma confinement parameters for the small solid flux conserver CTX [Phys. Fluids B 2, 1342 (1990)] spheromak can be determined, and confinement parameters for S-1 can be reevaluated, eliminating the previous confinement calculations assumption Ti(r)=Te(r). The CTX device has a calculated volume averaged beta <β> ranging from 3% to 8% and a volume averaged energy confinement time <τE> between 14 and 35 μsec; while the S-1 spheromak has <β> between 15% and 40% and <τE> ranging from 30 to 70 μsec. [ABSTRACT FROM AUTHOR]