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The application of biological organic fertilizer is one of the important measures to regulate the quality of tea. The application of organic fertilizer is helpful to increase the high quality of tea and regulate the soil physical and chemical properties and improve the soil environment. Then, the effects of different biological organic fertilizers on the growth quality of tea and the soil environment of tea garden were studied. The test results show that compared with the CK control group, T2 fertilization treatment is the most prominent in improving the polyphenols and caffeine content; meanwhile, T 6 fertilization treatment significantly increases the content of water extract and free amino acids of tea, which can effectively improve the composition of tea and improve the soil environment of tea garden. The application of six different organic fertilizers and different application amounts of the same organic fertilizer had significant differences on the content of tea water extract, tea polyphenols, free amino acids and caffeine.