47 results on '"Groten CJ"'
Search Results
2. Mitochondrial plasticity and synaptic plasticity crosstalk; in health and Alzheimer's disease.
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Sayehmiri, Fatemeh, Motamedi, Fereshteh, Batool, Zehra, Naderi, Nima, Shaerzadeh, Fatima, Zoghi, Anahita, Rezaei, Omidvar, Khodagholi, Fariba, and Pourbadie, Hamid Gholami
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NEUROPLASTICITY ,ALZHEIMER'S disease ,SYNAPTIC vesicles ,HOMEOSTASIS ,INTRACELLULAR calcium - Abstract
Synaptic plasticity is believed to underlie the cellular and molecular basis of memory formation. Mitochondria are one of the main organelles involved in metabolism and energy maintenance as plastic organelles that change morphologically and functionally in response to cellular needs and regulate synaptic function and plasticity through multiple mechanisms, including ATP generation, calcium homeostasis, and biogenesis. An increased neuronal activity enhances synaptic efficiency, during which mitochondria's spatial distribution and morphology change significantly. These organelles build up in the pre‐and postsynaptic zones to produce ATP, which is necessary for several synaptic processes like neurotransmitter release and recycling. Mitochondria also regulate calcium homeostasis by buffering intracellular calcium, which ensures proper synaptic activity. Furthermore, mitochondria in the presynaptic terminal have distinct morphological properties compared to dendritic or postsynaptic mitochondria. This specialization enables precise control of synaptic activity and plasticity. Mitochondrial dysfunction has been linked to synaptic failure in many neurodegenerative disorders, like Alzheimer's disease (AD). In AD, malfunctioning mitochondria cause delays in synaptic vesicle release and recycling, ionic gradient imbalances, and mostly synaptic failure. This review emphasizes mitochondrial plasticity's contribution to synaptic function. It also explores the profound effect of mitochondrial malfunction on neurodegenerative disorders, focusing on AD, and provides an overview of how they sustain cellular health under normal conditions and how their malfunction contributes to neurodegenerative diseases, highlighting their potential as a therapeutic target for such conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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3. Endoplasmic Reticulum and Mitochondrial Calcium Handling Dynamically Shape Slow Afterhyperpolarizations in Vasopressin Magnocellular Neurons.
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Kirchner, Matthew K., Althammer, Ferdinand, Campos-Lira, Elba, Montanez, Juliana, and Stern, Javier E.
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ENDOPLASMIC reticulum ,VASOPRESSIN ,CALCIUM channels ,POTASSIUM channels ,NEURONS ,MITOCHONDRIA ,CALCIUM ,HYPOTHALAMUS - Abstract
Many neurons including vasopressin (VP) magnocellular neurosecretory cells (MNCs) of the hypothalamic supraoptic nucleus (SON) generate afterhyperpolarizations (AHPs) during spiking to slow firing, a phenomenon known as spike frequency adaptation. The AHP is underlain by Ca
2+ -activated K+ currents, and while slow component (sAHP) features are well described, its mechanism remains poorly understood. Previous work demonstrated that Ca2+ influx through N-type Ca2+ channels is a primary source of sAHP activation in SON oxytocin neurons, but no obvious channel coupling was described for VP neurons. Given this, we tested the possibility of an intracellular source of sAHP activation, namely, the Ca2+ -handling organelles endoplasmic reticulum (ER) and mitochondria in male and female Wistar rats. We demonstrate that ER Ca2+ depletion greatly inhibits sAHPs without a corresponding decrease in Ca2+ signal. Caffeine sensitized AHP activation by Ca2+ . In contrast to ER, disabling mitochondria with CCCP or blocking mitochondria Ca2+ uniporters (MCUs) enhanced sAHP amplitude and duration, implicating mitochondria as a vital buffer for sAHPactivating Ca2+ . Block of mitochondria Na+ -dependent Ca2+ release via triphenylphosphonium (TPP+) failed to affect sAHPs, indicating that mitochondria Ca2+ does not contribute to sAHP activation. Together, our results suggests that ER Ca2+ -induced Ca2+ release activates sAHPs and mitochondria shape the spatiotemporal trajectory of the sAHP via Ca2+ buffering in VP neurons. Overall, this implicates organelle Ca2+ , and specifically ER-mitochondria-associated membrane contacts, as an important site of Ca2+ microdomain activity that regulates sAHP signaling pathways. Thus, this site plays a major role in influencing VP firing activity and systemic hormonal release. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. Activity- dependent mitochondrial ROS signaling regulates recruitment of glutamate receptors to synapses.
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Doser, Rachel L., Knight, Kaz M., Deihl, Ennis W., and Hoerndli, Frederic J.
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- 2024
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5. The TMEM16A channel as a potential therapeutic target in vascular disease.
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Al-Hosni, Rumaitha, Kaye, Rachel, Choi, Catherine Seoyoun, and Tammaro, Paolo
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- 2024
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6. Voltage- and Calcium-Gated Membrane Currents Tune the Plateau Potential Properties of Multiple Neuron Types.
- Author
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Neveu, Curtis L., Smolen, Paul, Baxter, Douglas A., and Byrne, John H.
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CALCIUM channels ,ACTION potentials ,STIMULUS intensity ,NEURONS ,CURVE fitting ,INFORMATION processing - Abstract
Many neurons exhibit regular firing that is limited to the duration and intensity of depolarizing stimuli. However, some neurons exhibit all-or-nothing plateau potentials that, once elicited, can lead to prolonged activity that is independent of stimulus intensity or duration. To better understand this diversity of information processing, we compared the voltage-gated and Ca
2+ -gated currents of three identified neurons from hermaphroditic Aplysia californica. Two of these neurons, B51 and B64, generated plateau potentials and a third neuron, B8, exhibited regular firing and was incapable of generating a plateau potential. With the exception of the Ca2+ -gated potassium current (IKCa ), all three neuron types expressed a similar array of outward and inward currents, but with distinct voltage-dependent properties for each neuron type. Inhibiting voltage-gated Ca2+ channels with Ni+ prolonged the plateau potential, indicating IKCa is important for plateau potential termination. In contrast, inhibiting persistent Na+ (INaP ) blocked plateau potentials, empirically and in simulations. Surprisingly, the properties and level of expression of INaP were similar in all three neurons, indicating that the presence of INaP does not distinguish between regular-firing neurons and neurons capable of generating plateau potentials. Rather, the key distinguishing factor is the relationship between INaP and outward currents such as the delayed outward current (ID ), and IKCa . We then demonstrated a technique for predicting complex physiological properties such as plateau duration, plateau amplitude, and action potential duration as a function of parameter values, by fitting a curve in parameter space and projecting the curve beyond the tested values. [ABSTRACT FROM AUTHOR]- Published
- 2023
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7. Use-dependent facilitation of electrical transmission involves changes to postsynaptic K+ current.
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Yueling Gu, Lee, Kelly H., Prosserman, Alex B., and Magoski, Neil S.
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ACTION potentials ,INHIBITORY postsynaptic potential ,CELL culture ,NEURONS ,HYPERPOLARIZATION (Cytology) ,NEUROENDOCRINE cells ,SYNCHRONIC order - Abstract
Activity-dependent modulation of electrical transmission typically involves Ca
2+ influx acting directly on gap junctions or initiating Ca2+ -dependent pathways that in turn modulate coupling. We now describe short-term use-dependent facilitation of electrical transmission between bag cell neurons from the hermaphroditic snail, Aplysia californica, that is instead mediated by changes in postsynaptic responsiveness. Bag cell neurons secrete reproductive hormone during a synchronous afterdischarge of action potentials coordinated by electrical coupling. Here, recordings from pairs of coupled bag cell neurons in culture showed that nonjunctional currents influence electrical transmission in a dynamic manner. Under a dual whole cell voltageclamp, the junctional current was linear and largely voltage-independent, while in current-clamp, the coupling coefficient was similar regardless of the extent of presynaptic hyperpolarization. Moreover, a train stimulus of action potential-like waveforms, in a voltage-clamped presynaptic neuron, elicited electrotonic potentials, in a current-clamped postsynaptic neuron, that facilitated over time when delivered at a frequency approximating the afterdischarge. Junctional current remained constant over the train stimulus, as did postsynaptic voltage-gated Ca2+ current. However, postsynaptic voltage-gated K+ current underwent cumulative inactivation, suggesting that K+ current run-down facilitates the electrotonic potential by boosting the response to successive junctional currents. Accordingly, preventing run-down by blocking postsynaptic K+ channels occluded facilitation. Finally, stimulation of bursts in coupled pairs resulted in synchronous firing, where active neurons could recruit silent partners through short-term use-dependent facilitation. Thus, potentiation of electrical transmission may promote synchrony in bag cell neurons and, by extension, reproductive function. [ABSTRACT FROM AUTHOR]- Published
- 2023
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8. Cholinergic depolarization recruits a persistent Ca2+ current in Aplysia bag cell neurons.
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Lee, Kelly H., Wassef, David E., MacNeil, Eammon K., and Magoski, Neil S.
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PROTEIN kinase C ,NEURONS ,INFERIOR colliculus ,CHOLINERGIC receptors ,ANIMAL sexual behavior ,NEUROENDOCRINE cells - Abstract
Many behaviors and types of information storage are mediated by lengthy changes in neuronal activity. In bag cell neurons of the hermaphroditic sea snail Aplysia californica, a transient cholinergic synaptic input triggers an ∼30-min afterdischarge. This causes these neuroendocrine cells to release egg laying hormone and elicit reproductive behavior. When acetylcholine is pressure-ejected onto a current-clamped bag cell neuron, the evoked depolarization is far longer than the current evoked by acetylcholine under voltage clamp, suggesting recruitment of another conductance. Our earlier studies found bag cell neurons to display a voltage-dependent persistent Ca
2+ current. Hence, we hypothesized that this current is activated by the acetylcholine-induced depolarization and sought a selective Ca2+ current blocker. Rapid Ca2+ current evoked by 200-ms depolarizing steps in voltage-clamped cultured bag cell neurons demonstrated a concentration-dependent sensitivity to Ni2+ , Co2+ , Zn2+ , and verapamil but not Cd2+ or ω-conotoxin GIVa. Leak subtraction of Ca2+ current evoked by 10-s depolarizing steps using the IC100 (concentration required to eliminate maximal current) of Ni2+ , Co2+ , Zn2+ , or verapamil revealed persistent Ca2+ current, demonstrating persistent current block. Only Co2+ and Zn2+ did not suppress the acetylcholine-induced current, although Zn2+ appeared to impact additional channels. When Co2+ was applied during an acetylcholine-induced depolarization, the amplitude was reduced; furthermore, protein kinase C activation, previously established to enhance the persistent Ca2+ current, extended the depolarization. Therefore, the persistent Ca2+ current sustains the acetylcholine-induced depolarization and may translate brief cholinergic input into afterdischarge initiation. This could be a general mechanism of triggering long-term change in activity with a short-lived input .NEW & NOTEWORTHY Ionotropic acetylcholine receptors mediate brief synaptic communication, including in bag cell neurons of the sea snail Aplysia. However, this study demonstrates that cholinergic depolarization can open a voltage-gated persistent Ca2+ current, which extends the bag cell neuron response to acetylcholine. Bursting in these neuroendocrine cells results in hormone release and egg laying. Thus, this emphasizes the role of ionotropic signaling in reaching a depolarized level to engage Ca2+ influx and perpetuating the activity necessary for behavior. [ABSTRACT FROM AUTHOR]- Published
- 2023
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9. Role of glia and extracellular matrix in controlling neuroplasticity in the central nervous system.
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Dzyubenko, Egor and Hermann, Dirk M.
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CENTRAL nervous system ,EXTRACELLULAR matrix ,NEUROPLASTICITY ,NEURAL circuitry ,NEURAL transmission ,BIOLOGICAL neural networks ,TISSUE remodeling ,GABA receptors - Abstract
Neuronal plasticity is critical for the maintenance and modulation of brain activity. Emerging evidence indicates that glial cells actively shape neuroplasticity, allowing for highly flexible regulation of synaptic transmission, neuronal excitability, and network synchronization. Astrocytes regulate synaptogenesis, stabilize synaptic connectivity, and preserve the balance between excitation and inhibition in neuronal networks. Microglia, the brain-resident immune cells, continuously monitor and sculpt synapses, allowing for the remodeling of brain circuits. Glia-mediated neuroplasticity is driven by neuronal activity, controlled by a plethora of feedback signaling mechanisms and crucially involves extracellular matrix remodeling in the central nervous system. This review summarizes the key findings considering neurotransmission regulation and metabolic support by astrocyte-neuronal networks, and synaptic remodeling mediated by microglia. Novel data indicate that astrocytes and microglia are pivotal for controlling brain function, indicating the necessity to rethink neurocentric neuroplasticity views. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Oxytocin-Modulated Ion Channel Ensemble Controls Depolarization, Integration and Burst Firing in CA2 Pyramidal Neurons.
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Jing-Jing Liu, Eyring, Katherine W., König, Gabriele M., Kostenis, Evi, and Tsien, Richard W.
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ION channels ,PYRAMIDAL neurons ,NEURAL circuitry ,POTASSIUM channels ,MEMBRANE potential ,COLLECTIVE memory - Abstract
Oxytocin (OXT) and OXT receptor (OXTR)-mediated signaling control excitability, firing patterns, and plasticity of hippocampal CA2 pyramidal neurons, which are pivotal in generation of brain oscillations and social memory. Nonetheless, the ionic mechanisms underlying OXTR-induced effects in CA2 neurons are not fully understood. Using slice physiology in a reporter mouse line and interleaved current-clamp and voltage-clamp experiments, we systematically identified the ion channels modulated by OXT signaling in CA2 pyramidal cells (PYRs) in mice of both sexes and explored how changes in channel conductance support altered electrical activity. Activation of OXTRs inhibits an outward potassium current mediated by inward rectifier potassium channels (I
Kir ) and thus favoring membrane depolarization. Concomitantly, OXT signaling also diminishes inward current mediated by hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels (Ih ), providing a hyperpolarizing drive. The combined reduction in both IKir and Ih synergistically elevate the membrane resistance and favor dendritic integration while the membrane potential is restrained from quickly depolarizing from rest. As a result, the responsiveness of CA2 PYRs to synaptic inputs is highly sharpened during OXTR activation. Unexpectedly, OXTR signaling also strongly enhances a tetrodotoxin-resistant (TTX-R), voltage-gated sodium current that helps drive the membrane potential to spike threshold and thus promote rhythmic firing. This novel array of OXTR-stimulated ionic mechanisms operates in close coordination and underpins OXT-induced burst firing, a key step in CA2 PYRs' contribution to hippocampal information processing and broader influence on brain circuitry. Our study deepens our understanding of underpinnings of OXT-promoted social memory and general neuropeptidergic control of cognitive states. [ABSTRACT FROM AUTHOR]- Published
- 2022
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11. Mitochondrial Ca2+ uptake by the MCU facilitates pyramidal neuron excitability and metabolism during action potential firing.
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Groten, Christopher J. and MacVicar, Brian A.
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ACTION potentials ,MITOCHONDRIA ,METABOLIC regulation ,METABOLISM ,ENERGY metabolism ,PYRAMIDAL neurons - Abstract
Neuronal activation is fundamental to information processing by the brain and requires mitochondrial energy metabolism. Mitochondrial Ca
2+ uptake by the mitochondrial Ca2+ uniporter (MCU) has long been implicated in the control of energy metabolism and intracellular Ca2+ signalling, but its importance to neuronal function in the brain remains unclear. Here, we used in situ electrophysiology and two-photon imaging of mitochondrial Ca2+ , cytosolic Ca2+ , and NAD(P)H to test the relevance of MCU activation to pyramidal neuron Ca2+ signalling and energy metabolism during action potential firing. We demonstrate that mitochondrial Ca2+ uptake by the MCU is tuned to enhanced firing rate and the strength of this relationship varied between neurons of discrete brain regions. MCU activation promoted electron transport chain activity and chemical reduction of NAD+ to NADH. Moreover, Ca2+ buffering by mitochondria attenuated cytosolic Ca2+ signals and thereby reduced the coupling between activity and the slow afterhyperpolarization, a ubiquitous regulator of excitability. Collectively, we demonstrate that the MCU is engaged by accelerated spike frequency to facilitate neuronal activity through simultaneous control of energy metabolism and excitability. As such, the MCU is situated to promote brain functions associated with high frequency signalling and may represent a target for controlling excessive neuronal activity. The importance of MCU-mediated mitochondrial Ca2+ uptake to the regulation of neuronal bioenergetics and excitability in the brain is demonstrated using single-cell patch-clamp recording and two-photon imaging. [ABSTRACT FROM AUTHOR]- Published
- 2022
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12. Amyloid pathology disrupts gliotransmitter release in astrocytes.
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Pillai, Anup Gopalakrishna and Nadkarni, Suhita
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GLUTAMATE receptors ,ASTROCYTES ,AMYLOID ,ALZHEIMER'S disease ,NEURAL transmission ,INTRACELLULAR calcium - Abstract
Accumulation of amyloid-beta (Aβ) is associated with synaptic dysfunction and destabilization of astrocytic calcium homeostasis. A growing body of evidence support astrocytes as active modulators of synaptic transmission via calcium-mediated gliotransmission. However, the details of mechanisms linking Aβ signaling, astrocytic calcium dynamics, and gliotransmission are not known. We developed a biophysical model that describes calcium signaling and the ensuing gliotransmitter release from a single astrocytic process when stimulated by glutamate release from hippocampal neurons. The model accurately captures the temporal dynamics of microdomain calcium signaling and glutamate release via both kiss-and-run and full-fusion exocytosis. We investigate the roles of two crucial calcium regulating machineries affected by Aβ: plasma-membrane calcium pumps (PMCA) and metabotropic glutamate receptors (mGluRs). When we implemented these Aβ-affected molecular changes in our astrocyte model, it led to an increase in the rate and synchrony of calcium events. Our model also reproduces several previous findings of Aβ associated aberrant calcium activity, such as increased intracellular calcium level and increased spontaneous calcium activity, and synchronous calcium events. The study establishes a causal link between previous observations of hyperactive astrocytes in Alzheimer's disease (AD) and Aβ-induced modifications in mGluR and PMCA functions. Analogous to neurotransmitter release, gliotransmitter exocytosis closely tracks calcium changes in astrocyte processes, thereby guaranteeing tight control of synaptic signaling by astrocytes. However, the downstream effects of AD-related calcium changes in astrocytes on gliotransmitter release are not known. Our results show that enhanced rate of exocytosis resulting from modified calcium signaling in astrocytes leads to a rapid depletion of docked vesicles that disrupts the crucial temporal correspondence between a calcium event and vesicular release. We propose that the loss of temporal correspondence between calcium events and gliotransmission in astrocytes pathologically alters astrocytic modulation of synaptic transmission in the presence of Aβ accumulation. Author summary: Signaling by astrocytes is critical to information processing at synapses, and its aberration plays a central role in neurological diseases, especially Alzheimer's disease (AD). A complete characterization of calcium signaling and the resulting pattern of gliotransmitter release from fine astrocytic processes are not accessible to current experimental tools. We developed a biophysical model that can quantitatively describe signaling by astrocytes in response to a wide range of synaptic activity. We show that AD-related molecular alterations disrupt the concurrence of calcium and gliotransmitter release events, a characterizing feature that enables astrocytes to influence synaptic signaling. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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13. Hydrogen peroxide and phosphoinositide metabolites synergistically regulate a cation current to influence neuroendocrine cell bursting.
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Chauhan‐Puri, Alamjeet K., Lee, Kelly H., and Magoski, Neil S.
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NEUROENDOCRINE cells ,PHOSPHOINOSITIDES ,HYDROGEN peroxide ,NICOTINAMIDE adenine dinucleotide phosphate ,PROTEIN kinase C ,PHOSPHOLIPASE C - Abstract
In various neurons, including neuroendocrine cells, non‐selective cation channels elicit plateau potentials and persistent firing. Reproduction in the marine snail Aplysia californica is initiated when the neuroendocrine bag cell neurons undergo an afterdischarge, that is, a prolonged period of enhanced excitability and spiking during which egg‐laying hormone is released into the blood. The afterdischarge is associated with both the production of hydrogen peroxide (H2O2) and activation of phospholipase C (PLC), which hydrolyses phosphatidylinositol‐4,5‐bisphosphate into diacylglycerol (DAG) and inositol trisphosphate (IP3). We previously demonstrated that H2O2 gates a voltage‐dependent cation current and evokes spiking in bag cell neurons. The present study tests if DAG and IP3 impact the H2O2‐induced current and excitability. In whole‐cell voltage‐clamped cultured bag cell neurons, bath‐application of 1‐oleoyl‐2‐acetyl‐sn‐glycerol (OAG), a DAG analogue, enhanced the H2O2‐induced current, which was amplified by the inclusion of IP3 in the pipette. A similar outcome was produced by the PLC activator, N‐(3‐trifluoromethylphenyl)‐2,4,6‐trimethylbenzenesulfonamide. In current‐clamp, OAG or OAG plus IP3, elevated the frequency of H2O2‐induced bursting. PKC is also triggered during the afterdischarge; when PKC was stimulated with phorbol 12‐myristate 13‐acetate, it caused a voltage‐dependent inward current with a reversal potential similar to the H2O2‐induced current. Furthermore, PKC activation followed by H2O2 reduced the onset latency and increased the duration of action potential firing. Finally, inhibiting nicotinamide adenine dinucleotide phosphate oxidase with 3‐benzyl‐7‐(2‐benzoxazolyl)thio‐1,2,3‐triazolo[4,5‐d]pyrimidine diminished evoked bursting in isolated bag cell neuron clusters. These results suggest that reactive oxygen species and phosphoinostide metabolites may synergize and contribute to reproductive behaviour by promoting neuroendocrine cell firing. Key points: Aplysia bag cell neurons secrete reproductive hormone during a lengthy burst of action potentials, known as the afterdischarge.During the afterdischarge, phospholipase C (PLC) hydrolyses phosphatidylinositol‐4,5‐bisphosphate into diacylglycerol (DAG) and inositol trisphosphate (IP3). Subsequent activation of protein kinase C (PKC) leads to H2O2 production.H2O2 evokes a voltage‐dependent inward current and action potential firing.Both a DAG analogue, 1‐oleoyl‐2‐acetyl‐sn‐glycerol (OAG), and IP3 enhance the H2O2‐induced current, which is mimicked by the PLC activator, N‐(3‐trifluoromethylphenyl)‐2,4,6‐trimethylbenzenesulfonamide.The frequency of H2O2‐evoked afterdischarge‐like bursting is augmented by OAG or OAG plus IP3.Stimulating PKC with phorbol 12‐myristate 13‐acetate shortens the latency and increases the duration of H2O2‐induced bursts.The nicotinamide adenine dinucleotide phosphate oxidase inhibitor, 3‐benzyl‐7‐(2‐benzoxazolyl)thio‐1,2,3‐triazolo[4,5‐d]pyrimidine, attenuates burst firing in bag cell neuron clusters. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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14. Organelle calcium-derived voltage oscillations in pacemaker neurons drive the motor program for food-seeking behavior in Aplysia.
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Bédécarrats, Alexis, Puygrenier, Laura, O'Byrne, John Castro, Lade, Quentin, Simmers, John, and Nargeot, Romuald
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- 2021
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15. Design of an Imaging Probe to Monitor Real-Time Redistribution of L-type Voltage-Gated Calcium Channels in Astrocytic Glutamate Signaling.
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Tabatabaee, Mitra Sadat, Kerkovius, Jeff, and Menard, Frederic
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CALCIUM channels ,GLUTAMIC acid ,CONOTOXINS ,CELL imaging ,FLUORIMETRY ,IMMUNOBLOTTING - Abstract
Purpose: In the brain, astrocytes are non-excitable cells that undergo rapid morphological changes when stimulated by the excitatory neurotransmitter glutamate. We developed a chemical probe to monitor how glutamate affects the density and distribution of astrocytic L-type voltage-gated calcium channels (LTCC). Procedures: The imaging probe FluoBar1 was created from a barbiturate ligand modified with a fluorescent coumarin moiety. The probe selectivity was examined with colocalization analyses of confocal fluorescence imaging in U118-MG and transfected COS-7 cells. Living cells treated with 50 nM FluoBar1 were imaged in real time to reveal changes in density and distribution of astrocytic LTCCs upon exposure to glutamate. Results: FluoBar1 was synthesized in ten steps. The selectivity of the probe was demonstrated with immunoblotting and confocal imaging of immunostained cells expressing the Ca
V 1.2 isoform of LTCCs proteins. Applying FluoBar1 to astrocyte model cells U118-MG allowed us to measure a fivefold increase in fluorescence density of LTCCs upon glutamate exposure. Conclusions: Imaging probe FluoBar1 allows the real-time monitoring of LTCCs in living cells, revealing for first time that glutamate causes a rapid increase of LTCC membranar density in astrocyte model cells. FluoBar1 may help tackle previously intractable questions about LTCC dynamics in cellular events. [ABSTRACT FROM AUTHOR]- Published
- 2021
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16. Local and CNS-Wide Astrocyte Intracellular Calcium Signaling Attenuation In Vivo with CalExflox Mice.
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Xinzhu Yu, Moye, Stefanie L., and Khakh, Baljit S.
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INTRACELLULAR calcium ,MICE ,MOTOR ability ,NEURAL circuitry ,ADULTS - Abstract
Astrocytes exist throughout the CNS and affect neural circuits and behavior through intracellular Ca
2+ signaling. Studying the function(s) of astrocyte Ca2+ signaling has proven difficult because of the paucity of tools to achieve selective attenuation. Based on recent studies, we generated and used male and female knock-in mice for Cre-dependent expression of mCherrytagged hPMCA2w/b to attenuate astrocyte Ca2+ signaling in genetically defined cells in vivo (CalExflox mice for Calcium Extrusion). We characterized CalExflox mice following local AAV-Cre microinjections into the striatum and found reduced astrocyte Ca2+ signaling (;90%) accompanied with repetitive self-grooming behavior. We also crossed CalExflox mice to astrocyte-specific Aldh1l1-Cre/ERT2 mice to achieve inducible global CNS-wide Ca2+ signaling attenuation. Within 6 d of induction in the bigenic mice, we observed significantly altered ambulation in the open field, disrupted motor coordination and gait, and premature lethality. Furthermore, with histologic, imaging, and transcriptomic analyses, we identified cellular and molecular alterations in the cerebellum following mCherry-tagged hPMCA2w/b expression. Our data show that expression of mCherry-tagged hPMCA2w/b with CalExflox mice throughout the CNS resulted in substantial attenuation of astrocyte Ca2+ signaling and significant behavioral alterations in adult mice. We interpreted these findings candidly in relation to the ability of CalEx to attenuate astrocyte Ca2+ signaling, with regards to additional mechanistic interpretations of the data, and their relation to past studies that reduced astrocyte Ca2+ signaling throughout the CNS. The data and resources provide complementary ways to interrogate the function(s) of astrocytes in multiple experimental scenarios. [ABSTRACT FROM AUTHOR]- Published
- 2021
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17. Whole brain irradiation in mice causes long-term impairment in astrocytic calcium signaling but preserves astrocyte-astrocyte coupling.
- Author
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Institoris, Adam, Murphy-Royal, Ciaran, Tarantini, Stefano, Yabluchanskiy, Andriy, Haidey, Jordan N., Csiszar, Anna, Ungvari, Zoltan, and Gordon, Grant R.
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COGNITION disorders ,CEREBRAL circulation ,LONG-term potentiation ,IRRADIATION ,ELECTRIC stimulation - Abstract
Whole brain irradiation (WBI) therapy is an important treatment for brain metastases and potential microscopic malignancies. WBI promotes progressive cognitive dysfunction in over half of surviving patients, yet, the underlying mechanisms remain obscure. Astrocytes play critical roles in the regulation of neuronal activity, brain metabolism, and cerebral blood flow, and while neurons are considered radioresistant, astrocytes are sensitive to γ-irradiation. Hallmarks of astrocyte function are the ability to generate stimulus-induced intercellular Ca
2+ signals and to move metabolic substrates through the connected astrocyte network. We tested the hypothesis that WBI-induced cognitive impairment associates with persistent impairment of astrocytic Ca2+ signaling and/or gap junctional coupling. Mice were subjected to a clinically relevant protocol of fractionated WBI, and 12 to 15 months after irradiation, we confirmed persistent cognitive impairment compared to controls. To test the integrity of astrocyte-to-astrocyte gap junctional coupling postWBI, astrocytes were loaded with Alexa-488-hydrazide by patch-based dye infusion, and the increase of fluorescence signal in neighboring astrocyte cell bodies was assessed with 2-photon microscopy in acute slices of the sensory-motor cortex. We found that WBI did not affect astrocyte-to-astrocyte gap junctional coupling. Astrocytic Ca2+ responses induced by bath administration of phenylephrine (detected with Rhod-2/AM) were also unaltered by WBI. However, an electrical stimulation protocol used in long-term potentiation (theta burst), revealed attenuated astrocyte Ca2+ responses in the astrocyte arbor and soma in WBI. Our data show that WBI causes a long-lasting decrement in synaptic-evoked astrocyte Ca2+ signals 12–15 months postirradiation, which may be an important contributor to cognitive decline seen after WBI. [ABSTRACT FROM AUTHOR]- Published
- 2021
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18. TrpML-mediated astrocyte microdomain Ca2+ transients regulate astrocyte-tracheal interactions.
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Ma, Zhiguo and Freeman, Marc R.
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- 2020
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19. Astrocyte-mediated spike-timing-dependent long-term depression modulates synaptic properties in the developing cortex.
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Manninen, Tiina, Saudargiene, Ausra, and Linne, Marja-Leena
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LONG-term synaptic depression ,SOMATOSENSORY cortex ,NEURAL transmission ,NEUROPLASTICITY ,PRESYNAPTIC receptors ,MOLECULAR dynamics ,SYNAPSES ,ASTROCYTES - Abstract
Astrocytes have been shown to modulate synaptic transmission and plasticity in specific cortical synapses, but our understanding of the underlying molecular and cellular mechanisms remains limited. Here we present a new biophysicochemical model of a somatosensory cortical layer 4 to layer 2/3 synapse to study the role of astrocytes in spike-timing-dependent long-term depression (t-LTD) in vivo. By applying the synapse model and electrophysiological data recorded from rodent somatosensory cortex, we show that a signal from a postsynaptic neuron, orchestrated by endocannabinoids, astrocytic calcium signaling, and presynaptic N-methyl-D-aspartate receptors coupled with calcineurin signaling, induces t-LTD which is sensitive to the temporal difference between post- and presynaptic firing. We predict for the first time the dynamics of astrocyte-mediated molecular mechanisms underlying t-LTD and link complex biochemical networks at presynaptic, postsynaptic, and astrocytic sites to the time window of t-LTD induction. During t-LTD a single astrocyte acts as a delay factor for fast neuronal activity and integrates fast neuronal sensory processing with slow non-neuronal processing to modulate synaptic properties in the brain. Our results suggest that astrocytes play a critical role in synaptic computation during postnatal development and are of paramount importance in guiding the development of brain circuit functions, learning and memory. Author summary: Brain development is dependent on neuroplasticity, the ability of the brain to modify its structure and function. Experimental evidence suggests that astrocytes, the non-neuronal cells in the brain, take part in shaping synaptic plasticity. In this study, we built a new computational model of spike-timing-dependent long-term depression and addressed the involvement of astroglial cells in modulation of synaptic glutamate transmission. Our results suggest that astrocytes are an integral part of synaptic computations and may guide brain circuit functions, learning and memory during postnatal development. Disruptions in these processes are likely involved in neurodevelopmental diseases such as schizophrenia and autism spectrum disorder. Modeling synaptic functions may help develop pharmacological targets for treatments of brain disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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20. Visualization of astrocytic intracellular Ca2+ mobilization.
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Okubo, Yohei and Iino, Masamitsu
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ENDOPLASMIC reticulum ,VISUALIZATION ,ASTROCYTES - Abstract
Astrocytes generate robust intracellular Ca2+ concentration changes (Ca2+ signals), which are assumed to regulate astrocytic functions that play crucial roles in the regulation of brain functions. One frequently used strategy for exploring the role of astrocytic Ca2+ signalling is the use of mice deficient in the type 2 inositol 1,4,5‐trisphosphate receptor (IP3R2). These IP3R2‐knockout (KO) mice are reportedly devoid of Ca2+ mobilization from the endoplasmic reticulum (ER) in astrocytes. However, they have shown no functional deficits in several studies, causing a heated debate as to the functional relevance of ER‐mediated Ca2+ signalling in astrocytes. Recently, the assumption that Ca2+ mobilization from the ER is absent in IP3R2‐KO astrocytes has been re‐evaluated using intraorganellar Ca2+ imaging techniques. The new results indicated that IP3R2‐independent Ca2+ release may generate Ca2+ nanodomains around the ER, which may help explain the absence of functional deficits in IP3R2‐KO mice. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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21. Hydrogen Peroxide Gates a Voltage-Dependent Cation Current in Aplysia Neuroendocrine Cells.
- Author
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Chauhan, Alamjeet K. and Magoski, Neil S.
- Subjects
NEUROENDOCRINE cells ,VOLTAGE-gated ion channels ,HYDROGEN peroxide ,NICOTINAMIDE adenine dinucleotide phosphate ,PROTEIN kinase C ,ANIMAL diversity - Abstract
Nonselective cation channels promote persistent spiking in many neurons from a diversity of animals. In the hermaphroditic marinesnail, Aplysia californica, synaptic input to the neuroendocrine bag cell neurons triggers various cation channels, causing an --30 min afterdischarge of action potentials and the secretion of egg-laying hormone. During the afterdischarge, protein kinase C is also activated, which in turn elevates hydrogen peroxide (H
2 02 ), likely by stimulating nicotinamide adenine dinucleotide phosphate oxidase. The present study investigated whether H2 02 regulates cation channels to drive the afterdischarge. In single, cultured bag cell neurons, H2 02 elicited a prolonged, concentration- and voltage-dependent inward current, associated with an increase in membrane conductance and a reversal potential of ~ + 30 mV. Compared with normal saline, the presence of Ca2+ -free, Na+ -free, or Na+ /Ca2+ -free extracellular saline, lowered the current amplitude and left-shifted the reversal potential, consistent with a nonselective cationic conductance. Preventing H2 02 reduction with the glutathione peroxidase inhibitor, mercaptosuccinate, enhanced the H2 02 -induced current, while boosting glutathione production with its precursor, JV-acetylcysteine, or adding the reducing agent, dithiothreitol, lessened the response. Moreover, the current generated by the alkylating agent, V-ethylmaleimide, occluded the effect of H2 02 . The H2 02 -induced current was inhibited by tetrodotoxin as well as the cation channel blockers, 9-phenanthrol and clotrimazole. In current-clamp, II, 02 stimulated burst firing, but this was attenuated or prevented altogether by the channel blockers. Finally, H2 02 evoked an afterdischarge from whole bag cell neuron clusters recorded ex vivo by sharp-electrode. H2 02 may regulate a cation channel to influence long-term changes in activity and ultimately reproduction. [ABSTRACT FROM AUTHOR]- Published
- 2019
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22. Biological effects of Ni(II) on monocytes and macrophages in normal and hyperglycemic environments.
- Author
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Chana, Monica, Lewis, Jill B., Davis, Ryan, Elam, Yolanda, Hobbs, David, Lockwood, Petra E., Wataha, John C., and Messer, Regina L.
- Abstract
Abstract: Corrosion and release of nickel ions from biomedical alloys are well documented, but little is still known about the effects of released nickel ions on cellular function with recurrent inflammatory challenges. Evidence suggests Ni(II) ions amplify LPS‐induced secretion of several pro‐inflammatory cytokines from monocytes. Exacerbating the inflammatory response, hyperglycemic conditions also affect monocytic function. This study investigated how Ni(II) and hyperglycemic conditions, both singly and in combination, alter monocyte proliferation, mitochondrial activity, inflammatory responses, and differentiation. Results showed that Ni(II) did not affect proliferation, but decreased mitochondrial activity in monocytic‐cells and macrophages under normal conditions. However, hyperglycemic conditions negated the toxicity seen with Ni(II) exposure. Cytokine secretion in response to LPS was variable, with little effect on IL6 secretion, but significantly increased secretion of IL1β at intermediate Ni(II) concentrations. Hyperglycemic conditions did not alter these results significantly. Finally, exposure to eluants from nickel‐based commercial alloys caused enhanced IL1β secretion from PMA‐treated cells. These data suggest that corrosion products from nickel‐containing dental alloys increased Ni(II)‐induced changes in cytokine secretion by monocytes and macrophages. By better defining the effects of Ni(II) at these lower, biomedically relevant concentrations, we improve understanding of the biomedical alloy risk in the context of dental inflammation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2433–2439, 2018. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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23. Spreading Depression Promotes Astrocytic Calcium Oscillations and Enhances Gliotransmission to Hippocampal Neurons.
- Author
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Wu, Dong Chuan, Chen, Rita Yu-Tzu, Cheng, Ting-Chun, Chiang, Yao-Chang, Shen, Mei-Lin, Hsu, Li-Ling, and Zhou, Ning
- Published
- 2018
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24. A Closely Associated Phospholipase C Regulates Cation Channel Function through Phosphoinositide Hydrolysis.
- Author
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Sturgeon, Raymond M. and Magoski, Neil S.
- Subjects
APLYSIA californica ,NEURONS ,HORMONES ,INOSITOL trisphosphate ,DIGLYCERIDES - Abstract
In the hemaphroditic sea snail, Aplysia californica, reproduction is initiated when the bag cell neurons secrete egg-laying hormone during a protracted afterdischarge. A source of depolarization for the afterdischarge is a voltage-gated, nonselective cation channel, similar to transient receptor potential (TRP) channels. Once the afterdischarge is triggered, phospholipase C (PLC) is activated to hydrolyze phosphatidylinositol- 4,5-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP
3 ). We previously reported that a DAG analog, 1-oleoyl-2-acetyl-sn-glycerol (OAG), activates a prominent, inward whole-cell cationic current that is enhanced by IP3 . To examine the underlying mechanism, we investigated the effect of exogenous OAG and IP3 , as well as PLC activation, on cation channel activity and voltage dependence in excised, inside-out patches from cultured bag cell neurons. OAG transiently elevated channel open probability (Po ) when applied to excised patches; however, coapplication of IP3 prolonged the OAG-induced response. In patches exposed to OAG and IP3 , channel voltage dependence was left-shifted; this was also observed with OAG, but not to the same extent. Introducing the PLC activator, m-3M3FBS, to patches increased channel Po , suggesting PLC may be physically linked to the channels. Accordingly, blocking PLC with U-73122 ablated the m-3M3FBS-induced elevation in Po . Treatment with m-3M3FBS left-shifted cation channel voltage dependence to a greater extent than exogenous OAG and IP3 . Finally, OAG and IP3 potentiated the stimulatory effect of PKC, which is also associated with the channel. Thus, the PLC-PKC signaling system is physically localized such that PIP2 breakdown products liberated during the afterdischarge modulate the cation channel and temporally influence neuronal activity. [ABSTRACT FROM AUTHOR]- Published
- 2018
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25. Protein Kinase C Enhances Electrical Synaptic Transmission by Acting on Junctional and Postsynaptic Ca2+ Currents.
- Author
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Beekharry, Christopher C., Yueling Gu, and Magoski, Neil S.
- Subjects
PROTEIN kinase C ,ACTION potentials ,GAP junctions (Cell biology) ,NEUROENDOCRINE cells ,VOLTAGE-clamp techniques (Electrophysiology) - Abstract
By synchronizing neuronal activity, electrical transmission influences the coordination, pattern, and/or frequency of firing. In the hemaphroditic marine-snail, Aplysia calfornica, the neuroendocrine bag cell neurons use electrical synapses to synchronize a 30 min afterdischarge of action potentials for the release of reproductive hormone. During the afterdischarge, protein kinase C (PKC) is activated, although its impact on bag cell neuron electrical transmission is unknown. This was investigated here by monitoring electrical synapses between paired cultured bag cell neurons using dual whole-cell recording. Voltage clamp revealed a largely voltage-independent junctional current, which was enhanced by treating with a PKC activator, PMA, before recording. We also examined the transfer of presynaptic action potential-like waveforms (generated in voltage clamp) to the postsynaptic cell (measured in current clamp). For control pairs, the presynaptic spike-like waveforms mainly evoked electrotonic potentials; however, when PKC was triggered, these stimuli consistently produced postsynaptic action potentials. To assess whether this involved changes to postsynaptic responsiveness, single bag cell neurons were injected with junctional-like current mimicking that evoked by a presynaptic action potential. Unlike control neurons, which were less likely to spike, cells in PMA always fired action potentials to the junctional-like current. Furthermore, PKC activation increased a postsynaptic voltage-gated Ca
2+ current, which was recruited even by modest depolarization associated with an electrotonic potential. Whereas PKC inhibits gap junctions in most systems, bag cell neurons are rather unique, as the kinase potentiates the electrical synapse; in turn, this synergizes with augmented postsynaptic Ca current to promote synchronous firing. [ABSTRACT FROM AUTHOR]2+ - Published
- 2018
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26. Role of Purinergic Receptor P2Y1 in Spatiotemporal Ca2+ Dynamics in Astrocytes.
- Author
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Shigetomi, Eiji, Hirayama, Yukiho J., Schuichi Koizumi, Kazuhiro Ikenaka, and Tanaka, Kenji F.
- Subjects
ASTROCYTES ,PURINERGIC receptors ,SYNAPSES ,CALCIUM ,SPATIOTEMPORAL processes - Abstract
Fine processes of astrocytes enwrap synapses and are well positioned to sense neuronal information via synaptic transmission. In rodents, astrocyte processes sense synaptic transmission via Gq-protein coupled receptors (GqPCR), including the P2Y1 receptor (P2Y1R), to generate Ca
2+ signals. Astrocytes display numerous spontaneous microdomain Ca2+ signals; however, it is not clear whether such signals are due to local synaptic transmission and/or in what timeframe astrocytes sense local synaptic transmission. To ask whether GqPCRs mediate microdomain Ca2+ signals, we engineered mice (both sexes) to specifically overexpress P2Y1Rs in astrocytes, and we visualized Ca2+ signals via a genetically encoded Ca2+ indicator, GCaMP6f, in astrocytes from adult mice. Astrocytes overexpressing P2YlRs showed significantly larger Ca2+ signals in response to exogenously applied ligand and to repetitive electrical stimulation of axons compared with controls. However, we found no evidence of increased microdomain Ca2+ signals. Instead, Ca2+ waves appeared and propagated to occupy areas that were up to 80-fold larger than microdomain Ca2+ signals. These Ca2+ waves accounted for only 2% of total Ca2+ events, but they were 1.9-fold larger and 2.9-fold longer in duration than microdomain Ca2+ signals at processes. Ca2+ waves did not require action potentials for their generation and occurred in a probenecid-sensitive manner, indicating that the endogenous ligand for P2Y1R is elevated independently of synaptic transmission. Our data suggest that spontaneous microdomain Ca2+ signals occur independently of P2Y1R activation and that astrocytes may not encode neuronal information in response to synaptic transmission at a point source of neurotransmitter release. [ABSTRACT FROM AUTHOR]- Published
- 2018
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27. Gliotransmission: Beyond Black-and-White.
- Author
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Savtchouk, Iaroslav and Volterra, Andrea
- Subjects
NEUROSCIENCES ,NEURAL circuitry ,NEURONS ,NEURAL development ,BRAIN imaging - Abstract
Astrocytes are highly complex cells with many emerging putative roles in brain function. Of these, gliotransmission (active information transfer from glia to neurons) has probably the widest implications on our understanding of how the brain works: do astrocytes really contribute to information processing within the neural circuitry? "Positive evidence" for this stems from work of multiple laboratories reporting many examples of modulatory chemical signaling from astrocytes to neurons in the timeframe of hundreds of milliseconds to several minutes. This signaling involves, but is not limited to, Ca
2+ -dependent vesicular transmitter release, and results in a variety of regulatory effects at synapses in many circuits that are abolished by preventing Ca2+ elevations or blocking exocytosis selectively in astrocytes. In striking contradiction, methodologically advanced studies by a few laboratories produced "negative evidence," triggering a heated debate on the actual existence and properties of gliotransmission. In this context, a skeptics' camp arose, eager to dismiss the whole positive evidence based on a number of assumptions behind the negative data, such as the following: (1) deleting a single Ca2+ release pathway (IP3R2) removes all the sources for Ca2+ -dependent gliotransmission; (2) stimulating a transgenically expressed Gq- GPCR (MrgAl) mimics the physiological Ca2+ signaling underlying gliotransmitter release; (3) age-dependent downregulation of an endogenous GPCR (mGluR5) questions gliotransmitter release in adulthood; and (4) failure by transcriptome analysis to detect vGluts or canonical synaptic SNAREs in astrocytes proves inexistence/functional irrelevance of vesicular gliotransmitter release. We here discuss how the above assumptions are likely wrong and oversimplistic. In light of the most recent literature, we argue that gliotransmission is a more complex phenomenon than originally thought, possibly consisting of multiple forms and signaling processes, whose correct study and understanding require more sophisticated tools and finer scientific experiments than done until today. Under this perspective, the opposing camps can be reconciled and the field moved forward. Along the path, a more cautious mindset and an attitude to open discussion and mutual respect between opponent laboratories will be good companions. [ABSTRACT FROM AUTHOR]- Published
- 2018
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28. Amendments and Corrections to Mattusch et al. (Anesthesiology 2015; 122[5]: 1047-59), "Impact of Hyperpolarization-activated, Cyclic Nucleotide-gated Cation Channel Type 2 for the Xenon-mediated Anesthetic Effect: Evidence from In Vitro and In Vivo Experiments".
- Author
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Kratzer, Stephan, Haseneder, Rainer, Goldstein, Peter A., Kochs, Eberhard, and Rammes, Gerhard
- Published
- 2017
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29. Steady-State Free Ca2+ in Astrocytes Is Decreased by Experience and Impacts Arteriole Tone.
- Author
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Mehina, Eslam M. F., Murphy-Royal, Ciaran, and Gordon, Grant R.
- Subjects
ASTROCYTES ,NEUROPLASTICITY ,TWO-photon-spectroscopy ,SOMATOSENSORY cortex ,GLUTAMATE receptors - Abstract
Astrocytes can control basal synaptic strength and arteriole tone via their resting Ca
2+ activity. However, whether resting astrocyte Ca2+ can adjust to a new steady-state level, with an impact on surrounding brain cells, remains unknown. Using two-photon Ca2+ imaging in male rat acute brain slices of the somatosensory neocortex, we found that theta burst neural activity produced an unexpected long-lasting reduction in astrocyte free Ca2+ in the soma and endfeet. The drop in intracellular Ca2+ was attenuated by antagonists targeting multiple ionotropic and metabotropic glutamate receptors, and intracellular cascades involved Ca2+ stores and nitric oxide. The reduction in astrocyte endfoot Ca2+ was coincident with an increase in arteriole tone, and both the Ca2+ drop and the tone change were prevented by an NMDA receptor antagonist. Astrocyte patch-clamp experiments verified that the glutamate receptors in question were located on astrocytes and that Ca2+ changes within astrocytes were responsible for the long-lasting change in arteriole diameter caused by theta burst neural activity. In astrocytes from animals that lived in an enriched environment, we measured a relatively lower resting Ca2+ level that occluded any further drop in Ca2+ in response to theta burst activity. These data suggest that electrically evoked patterns of neural activity or natural experience can adjust steady-state resting astrocyte Ca2+ and that the effect has an impact on basal arteriole diameter. [ABSTRACT FROM AUTHOR]- Published
- 2017
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30. Comparison of GCaMP3 and GCaMP6f for studying astrocyte Ca2+ dynamics in the awake mouse brain.
- Author
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Ye, Liang, Haroon, Mateen A., Salinas, Angelica, and Paukert, Martin
- Subjects
BRAIN physiology ,ASTROCYTES ,CALCIUM ions ,GREEN fluorescent protein ,LABORATORY mice - Abstract
In recent years it has become increasingly clear that astrocytes play a much more active role in neural processes than the traditional view of them as supporting cells suggests. Although not electrically excitable, astrocytes exhibit diverse Ca
2+ dynamics across spatial and temporal scales, more or less dependent on the animal's behavioral state. Ca2+ dynamics range from global elevations lasting multiple seconds encompassing the soma up to the finest processes, to short elevations restricted to so-called microdomains within fine processes. Investigations of astrocyte Ca2+ dynamics have particularly benefitted from the development of Genetically-Encoded Calcium Indicators (GECIs). GECI expression can be achieved non-invasively in a cell type-specific manner and it can be genetically targeted to subcellular domains. The GCaMP family, a group of GECIs derived from the green fluorescent protein, has experienced some of the fastest advancements during the past decade. As a consequence we are now facing the challenge of needing to compare published data obtained with different versions of GECIs. With the intention to provide some guidance, here we compared Ca2+ dynamics across scales in awake transgenic mice expressing either the well-established GCaMP3, or the increasingly popular GCaMP6f, specifically in astrocytes. We found that locomotion-induced global Ca2+ elevations in cortical astrocytes displayed only minor kinetic differences and their apparent dynamic ranges for Ca2+ sensing were not different. In contrast, Ca2+ waves in processes and microdomain Ca2+ transients were much more readily detectable with GCaMP6f. Our findings suggest that behavioral state-dependent global astrocyte Ca2+ responses can be studied with either GCaMP3 or GCaMP6f whereas the latter is more appropriate for studies of spatially restricted weak and fast Ca2+ dynamics. [ABSTRACT FROM AUTHOR]- Published
- 2017
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31. TRPA1 channels promote astrocytic Ca2+ hyperactivity and synaptic dysfunction mediated by oligomeric forms of amyloid-β peptide.
- Author
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Bosson, Anthony, Paumier, Adrien, Boisseau, Sylvie, Jacquier-Sarlin, Muriel, Buisson, Alain, and Albrieux, Mireille
- Subjects
ALZHEIMER'S disease ,AMYLOID ,SENILE dementia ,GLYCOPROTEINS ,NEURONS - Abstract
Background: Excessive synaptic loss is thought to be one of the earliest events in Alzheimer's disease (AD). However, the key mechanisms that maintain plasticity of synapses during adulthood or initiate synapse dysfunction in AD remain unknown. Recent studies suggest that astrocytes contribute to functional changes observed during synaptic plasticity and play a major role in synaptic dysfunction but astrocytes behavior and involvement in early phases of AD remained largely undefined. Methods: We measure astrocytic calcium activity in mouse CA1 hippocampus stratum radiatum in both the global astrocytic population and at a single cell level, focusing in the highly compartmentalized astrocytic arbor. Concurrently, we measure excitatory post-synaptic currents in nearby pyramidal neurons. Results: We find that application of soluble Aβ oligomers (Aβo) induced fast and widespread calcium hyperactivity in the astrocytic population and in the microdomains of the astrocyte arbor. We show that astrocyte hyperactivity is independent of neuronal activity and is repaired by transient receptor potential A1 (TRPA1) channels blockade. In return, this TRPA1 channels-dependent hyperactivity influences neighboring CA1 neurons triggering an increase in glutamatergic spontaneous activity. Interestingly, in an AD mouse model (APP/PS1-21 mouse), astrocyte calcium hyperactivity equally takes place at the beginning of Aβ production, depends on TRPA1 channels and is linked to CA1 neurons hyperactivity. Conclusions: Our experiments demonstrate that astrocytes contribute to early Aβo toxicity exhibiting a global and local Ca
2+ hyperactivity that involves TRPA1 channels and is related to neuronal hyperactivity. Together, our data suggest that astrocyte is a frontline target of Aβo and highlight a novel mechanism for the understanding of early synaptic dysregulation induced by soluble Aβo species. [ABSTRACT FROM AUTHOR]- Published
- 2017
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32. Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP.
- Author
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Sherwood, Mark William, Arizono, Misa, Hisatsune, Chihiro, Bannai, Hiroko, Ebisui, Etsuko, Sherwood, John Lawrence, Panatier, Aude, Oliet, Stéphane Henri Richard, and Mikoshiba, Katsuhiko
- Published
- 2017
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33. Mitochondrial calcium homeostasis: Implications for neurovascular and neurometabolic coupling.
- Author
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Kannurpatti, Sridhar S.
- Abstract
Mitochondrial function is critical to maintain high rates of oxidative metabolism supporting energy demands of both spontaneous and evoked neuronal activity in the brain. Mitochondria not only regulate energy metabolism, but also influence neuronal signaling. Regulation of ‘‘energy metabolism’’ and ‘‘neuronal signaling’’ (i.e. neurometabolic coupling), which are coupled rather than independent can be understood through mitochondria’s integrative functions of calcium ion (Ca
2+ ) uptake and cycling. While mitochondrial Ca2+ do not affect hemodynamics directly, neuronal activity changes are mechanistically linked to functional hyperemic responses (i.e. neurovascular coupling). Early in vitro studies lay the foundation of mitochondrial Ca2+ homeostasis and its functional roles within cells. However, recent in vivo approaches indicate mitochondrial Ca2+ homeostasis as maintained by the role of mitochondrial Ca2+ uniporter (mCU) influences system-level brain activity as measured by a variety of techniques. Based on earlier evidence of subcellular cytoplasmic Ca2+ microdomains and cellular bioenergetic states, a mechanistic model of Ca2+ mobilization is presented to understand systems-level neurovascular and neurometabolic coupling. This integrated view from molecular and cellular to the systems level, where mCU plays a major role in mitochondrial and cellular Ca2+ homeostasis, may explain the wide range of activation-induced coupling across neuronal activity, hemodynamic, and metabolic responses. [ABSTRACT FROM AUTHOR]- Published
- 2017
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34. Diacylglycerol-mediated regulation of Aplysia bag cell neuron excitability requires protein kinase C.
- Author
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Sturgeon, Raymond M. and Magoski, Neil S.
- Subjects
DIGLYCERIDES ,APLYSIA ,NEURONS ,PROTEIN kinase C ,PHOSPHOINOSITIDES - Abstract
Key points In Aplysia, reproduction is initiated by the bag cell neurons and a prolonged period of enhanced excitability known as the afterdischarge., Phosphoinositide turnover is upregulated during the afterdischarge resulting in the hydrolysis of phosphatidylinositol-4,5-bisphosphate by phospholipase C (PLC) and the release of diacylglycerol (DAG) and inositol trisphosphate (IP
3 )., In whole-cell voltage-clamped cultured bag cell neurons, 1-oleoyl-2-acetyl- sn-glycerol (OAG), a synthetic DAG analogue, activates a dose-dependent, transient, inward current ( IOAG ) that is enhanced by IP3 , mimicked by PLC activation and dependent on basal protein kinase C (PKC) activity., OAG depolarizes bag cell neurons and triggers action potential firing in culture, and prolongs electrically stimulated afterdischarges in intact bag cell neuron clusters ex vivo., Although PKC alone cannot activate the current, it is required for IOAG ; this is the first description of required obligate PKC activity working in concert with PLC, DAG and IP3 to maintain the depolarization required for prolonged excitability in Aplysia reproduction., Abstract Following synaptic input, the bag cell neurons of Aplysia undergo a long-term afterdischarge of action potentials to secrete egg-laying hormone and initiate reproduction. Early in the afterdischarge, phospholipase C (PLC) hydrolyses phosphatidylinositol-4,5-bisphosphate into inositol trisphosphate (IP3 ) and diacylglycerol (DAG). In Aplysia, little is known about the action of DAG, or any interaction with IP3 ; thus, we examined the effects of a synthetic DAG analogue, 1-oleoyl-2-acetyl- sn-glycerol (OAG), on whole-cell voltage-clamped cultured bag cell neurons. OAG induced a large, prolonged, Ca2+ -permeable, concentration-dependent inward current ( IOAG ) that reversed at ∼−20 mV and was enhanced by intracellular IP3 . A similar current was evoked by either another DAG analogue, 1,2-dioctanoyl- sn-glycerol (DOG), or activating PLC with N-(3-trifluoromethylphenyl)-2,4,6-trimethylbenzenesulfonamide ( m-3M3FBS). IOAG was reduced by the general cation channel blockers Gd3+ or flufenamic acid. Work in other systems indicated that OAG activates channels independently of protein kinase C (PKC); however, we found pretreating bag cell neurons with any of the PKC inhibitors bisindolylmaleimide, sphinganine, or H7, attenuated IOAG . However, stimulating PKC with phorbol 12-myristate 13-acetate (PMA) did not evoke current or enhance IOAG ; moreover, unlike PMA, OAG failed to trigger PKC, as confirmed by an independent bioassay. Finally, OAG or m-3M3FBS depolarized cultured neurons, and while OAG did not provoke afterdischarges from bag cell neurons in the nervous system, it did double the duration of synaptically elicited afterdischarges. To our knowledge, this is the first report of obligate PKC activity for IOAG gating. An interaction between phosphoinositol metabolites and PKC could control the cation channel to influence afterdischarge duration. [ABSTRACT FROM AUTHOR]- Published
- 2016
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35. Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells.
- Author
-
Groten, Christopher J., Rebane, Jonathan T., Hodgson, Heather M., Chauhan, Alamjeet K., Blohm, Gunnar, and Magoski, Neil S.
- Abstract
After Ca
2+ influx, mitochondria can sequester Ca2+ and subsequently release it back into the cytosol. This form of Ca2+ -induced Ca2+ release (CICR) prolongs Ca2+ signaling and can potentially mediate activity-dependent plasticity. As Ca2+ is required for its subsequent release, Ca2+ removal systems, like the plasma membrane Ca2+ -ATPase (PMCA), could impact CICR. Here we examine such a role for the PMCA in the bag cell neurons of Aplysia californica. CICR is triggered in these neurons during an afterdischarge and is implicated in sustaining membrane excitability and peptide secretion. Somatic Ca2+ was measured from fura-PE3-loaded cultured bag cell neurons recorded under whole cell voltage clamp. Voltage-gated Ca2+ influx was elicited with a 5-Hz, 1-min train, which mimics the fast phase of the afterdischarge. PMCA inhibition with carboxyeosin or extracellular alkalization augmented the effectiveness of Ca2+ influx in eliciting mitochondrial CICR. A Ca2+ compartment model recapitulated these findings and indicated that disrupting PMCA-dependent Ca2+ removal increases CICR by enhancing mitochondrial Ca2+ loading. Indeed, carboxyeosin augmented train-evoked mitochondrial Ca2+ uptake. Consistent with their role on Ca2+ dynamics, cell labeling revealed that the PMCA and mitochondria overlap with Ca2+ entry sites. Finally, PMCA-dependent Ca2+ extrusion did not impact endoplasmic reticulum-dependent Ca2+ removal or release, despite the organelle residing near Ca2+ entry sites. Our results demonstrate that Ca2+ removal by the PMCA influences the propensity for stimulus-evoked CICR by adjusting the amount of Ca2+ available for mitochondrial Ca2+ uptake. This study highlights a mechanism by which the PMCA could impact activity-dependent plasticity in the bag cell neurons. [ABSTRACT FROM AUTHOR]- Published
- 2016
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36. Nicotine inhibits potassium currents in Aplysia bag cell neurons.
- Author
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White, Sean H., Sturgeon, Raymond M., and Magoski, Neil S.
- Published
- 2016
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37. F-actin links Epac-PKC signaling to purinergic P2X3 receptor sensitization in dorsal root ganglia following inflammation.
- Author
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Yanping Gu, Congying Wang, GuangWen Li, and Huang, Li-Yen M.
- Subjects
F-actin ,PROTEIN kinase C ,PURINERGIC receptors ,DORSAL root ganglia ,INFLAMMATION ,CELLULAR signal transduction - Abstract
Sensitization of purinergic P2X3 receptors (P2X3Rs) contributes to the production of exaggerated nociceptive responses following inflammatory injury. We showed previously that prostaglandin E2 (PGE2) potentiates P2X3R-mediated ATP currents in dorsal root ganglion neurons isolated from both control and complete Freund's adjuvant-induced inflamed rats. PGE2 potentiation of ATP currents depends only on PKA signaling in control neurons, but it depends on both PKA and PKC signaling in inflamed neurons. We further found that inflammation evokes an increase in exchange proteins directly activated by cAMP (Epacs) in dorsal root ganglions. This increase promotes the activation of PKC to produce a much enhanced PGE2 effect on ATP currents and to elicit Epac-dependent flinch nocifensive behavioral responses in complete Freund's adjuvant rats. The link between Epac-PKC signaling and P2X3R sensitization remains unexplored. Here, we show that the activation of Epacs promotes the expression of phosphorylated PKC and leads to an increase in the cytoskeleton, F-actin, expression at the cell perimeter. Depolymerization of F-actin blocks PGE2-enhanced ATP currents and inhibits P2X3R-mediated nocifensive responses after inflammation. Thus, F-actin is dynamically involved in the Epac-PKC-dependent P2X3R sensitization. Furthermore, Epacs induce a PKC-dependent increase in the membrane expression of P2X3Rs. This increase is abolished by F-actin depolymerization, suggesting that F-actin mediates Epac-PKC signaling of P2X3R membrane expression. Thus, after inflammation, an Epac-PKC dependent increase in F-actin in dorsal root ganglion neurons enhances the membrane expression of P2X3Rs to bring about sensitization of P2X3Rs and abnormal pain behaviors. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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38. Persistent Associative Plasticity at an Identified Synapse Underlying Classical Conditioning Becomes Labile with Short-Term Homosynaptic Activation.
- Author
-
Jiangyuan Hu and Schacher, Samuel
- Subjects
NEUROPLASTICITY ,SYNAPSES ,CLASSICAL conditioning ,APLYSIA californica ,SEROTONIN ,PROTEIN kinase C - Abstract
Synapses express different forms of plasticity that contribute to different forms of memory, and both memory and plasticity can become labile after reactivation. We previously reported that a persistent form of nonassociative long-term facilitation (PNA-LTF) of the sensorimotor synapses in Aplysia californica, a cellular analog of long-term sensitization, became labile with short-term heterosynaptic reactivation and reversed when the reactivation was followed by incubation with the protein synthesis inhibitor rapamycin. Here we examined the reciprocal impact of different forms of short-term plasticity (reactivations) on a persistent form of associative long-term facilitation (PA-LTF), a cellular analog of classical conditioning, which was expressed at Aplysia sensorimotor synapses when a tetanic stimulation of the sensory neurons was paired with a brief application of serotonin on 2 consecutive days. The expression of short-term homosynaptic plasticity [post-tetanic potentiation or homosynaptic depression (HSD)], or short-term heterosynaptic plasticity [serotonin-induced facilitation or neuropeptide Phe-Met-Arg-Phe-NH
2 (FMRFa)-induced depression], at synapses expressing PA-LTF did not affect the maintenance of PA-LTF. The kinetics of HSD was attenuated at synapses expressing PA-LTF, which required activation of protein kinase C (PKC). Both PA-LTF and the attenuated kinetics of HSD were reversed by either a transient blockade of PKC activity or a homosynaptic, but not heterosynaptic, reactivation when paired with rapamycin. These results indicate that two different forms of persistent synaptic plasticity, PA-LTF and PNA-LTF, expressed at the same synapse become labile when reactivated by different stimuli. [ABSTRACT FROM AUTHOR]- Published
- 2015
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39. PKC Enhances the Capacity for Secretion by Rapidly Recruiting Covert Voltage-Gated Ca2+ Channels to the Membrane.
- Author
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Groten, Christopher J. and Magoski, Neil S.
- Subjects
VOLTAGE-gated ion channels ,CALCIUM channels ,PROTEIN kinase C ,BIOLOGICAL transport ,APLYSIA californica ,PHYSIOLOGY - Abstract
It is unknown whether neurons can dynamically control the capacity for secretion by promptly changing the number of plasma membrane voltage-gated Ca
2+ channels. To address this, we studied peptide release from the bag cell neurons of Aplysia californica, which initiate reproduction by secreting hormone during an after discharge. This burst engages protein kinase C (PKC) to trigger the insertion of a covert Ca2+ channel, Apl Cav 2, alongside a basal channel, Apl Cav l. The significance of Apl Cav 2 recruitment to secretion remains undetermined; therefore, we used capacitance tracking to assay secretion, along with Ca2+ imaging and Ca2+ current measurements, from cultured bag cell neurons under whole-cell voltage-clamp. Activating PKC with the phorbol ester, PMA, enhanced Ca2+ entry, and potentiated stimulus-evoked secretion. This relied on channel insertion, as it was occluded by preventing Apl Cav 2 engagement with prior whole-cell dialysis or the cytoskeletal toxin, latrunculin B. Channel insertion reduced the stimulus duration and/or frequency required to initiate secretion and strengthened excitation-secretion coupling, indicating that Apl Cav 2 accesses peptide release more readily than Apl Cav l. The coupling of Apl Cav 2 to secretion also changed with behavioral state, as Apl Cav 2 failed to evoke secretion in silent neurons from reproductively inactive animals. Finally, PKC also acted secondarily to enhance prolonged exocytosis triggered by mitochondrial Ca2+ release. Collectively, our results suggest that bag cell neurons dynamically elevate Ca2+ channel abundance in the membrane to ensure adequate secretion during the after discharge. [ABSTRACT FROM AUTHOR]- Published
- 2015
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40. Ca2+-induced uncoupling of Aplysia bag cell neurons.
- Author
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Dargaei, Zahra, Standage, Dominic, Groten, Christopher J., Blohm, Gunnar, and Magoski, Neil S.
- Subjects
APLYSIA ,NEURAL circuitry ,CALCIUM ions ,NEUROENDOCRINE cells ,INTRACELLULAR calcium ,PROTEIN kinase C ,ELECTRIC power transmission - Abstract
Electrical transmission is a dynamically regulated form of communication and key to synchronizing neuronal activity. The bag cell neurons of Aplysia are a group of electrically coupled neuroendocrine cells that initiate ovulation by secreting egg-laying hormone during a prolonged period of synchronous firing called the afterdischarge. Accompanying the afterdischarge is an increase in intracellular Ca
2+ and the activation of protein kinase C (PKC). We used whole cell recording from paired cultured bag cell neurons to demonstrate that electrical coupling is regulated by both Ca2+ and PKC. Elevating Ca2+ with a train of voltage steps, mimicking the onset of the afterdischarge, decreased junctional current for up to 30 min. Inhibition was most effective when Ca2+ entry occurred in both neurons. Depletion of Ca2+ from the mitochondria, but not the endoplasmic reticulum, also attenuated the electrical synapse. Buffering Ca2+ with high intracellular EGTA or inhibiting calmodulin kinase prevented uncoupling. Furthermore, activating PKC produced a small but clear decrease in junctional current, while triggering both Ca2+ influx and PKC inhibited the electrical synapse to a greater extent than Ca2+ alone. Finally, the amplitude and time course of the postsynaptic electrotonic response were attenuated after Ca2+ influx. A mathematical model of electrically connected neurons showed that excessive coupling reduced recruitment of the cells to fire, whereas less coupling led to spiking of essentially all neurons. Thus a decrease in electrical synapses could promote the afterdischarge by ensuring prompt recovery of electrotonic potentials or making the neurons more responsive to current spreading through the network. [ABSTRACT FROM AUTHOR]- Published
- 2015
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41. Electrical coupling between Aplysia bag cell neurons: characterization and role in synchronous firing.
- Author
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Dargaei, Zahra, Colmers, Phillip L. W., Hodgson, Heather M., and Magoski, Neil S.
- Subjects
APLYSIA californica ,NEUROENDOCRINE cells ,ACTION potentials ,GAP junctions (Cell biology) ,NEUROPEPTIDES ,PHYSIOLOGY - Abstract
In neuroendocrine cells, hormone release often requires a collective burst of action potentials synchronized by gap junctions. This is the case for the electrically coupled bag cell neurons in the reproductive system of the marine snail, Aplysia californica. These neuroendocrine cells are found in two clusters, and fire a synchronous burst, called the afterdischarge, resulting in neuropeptide secretion and the triggering of ovulation. However, the physiology and pharmacology of the bag cell neuron electrical synapse are not completely understood. As such, we made dual whole cell recordings from pairs of electrically coupled cultured bag cell neurons. The junctional current was nonrectifying and not influenced by postsynaptic voltage. Furthermore, junctional conductance was voltage independent and, not surprisingly, strongly correlated with coupling coefficient magnitude. The electrical synapse also acted as a low-pass filter, although under certain conditions, electrotonic potentials evoked by presynaptic action potentials could drive postsynaptic spikes. If coupled neurons were stimulated to spike simultaneously, they presented a high degree of action potential synchrony compared with not-coupled neurons. The electrical synapse failed to pass various intracellular dyes, but was permeable to Cs
+ , and could be inhibited by niflumic acid, meclofenamic acid, or 5-nitro-2-(3-phenylpropylamino)benzoic acid. Finally, extracellular and sharp-electrode recording from the intact bag cell neuron cluster showed that these pharmacological uncouplers disrupted both electrical coupling and afterdischarge generation in situ. Thus electrical synapses promote bag cell neuron firing synchrony and may allow for electrotonic spread of the burst through the network, ultimately contributing to propagation of the species. [ABSTRACT FROM AUTHOR]- Published
- 2014
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42. A potentially novel nicotinic receptor in Aplysia neuroendocrine cells.
- Author
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White, Sean H., Carter, Christopher J., and Magoski, Neil S.
- Subjects
NICOTINIC receptors ,APLYSIA ,NEUROENDOCRINE cells ,LIGANDS (Biochemistry) ,NEURAL transmission ,ACETYLCHOLINE ,POLYMERASE chain reaction - Abstract
Nicotinic receptors form a diverse group of ligand-gated ionotropic receptors with roles in both synaptic transmission and the control of excitability. In the bag cell neurons of Aplysia, acetylcholine activates an ionotropic receptor, which passes inward current to produce a long-lasting afterdischarge and hormone release, leading to reproduction. While testing the agonist profile of the cholinergic response, we observed a second current that appeared to be gated only by nicotine and not acetylcholine. The peak nicotine-evoked current was markedly smaller in magnitude than the acetylcholine-induced current, cooperative (Hill value of 2.7), had an EC
50 near 500 µM, readily recovered from desensitization, showed Ca2+ permeability, and was blocked by mecamylamine, dihydro-β-erythroidine, or strychnine, but not by α-conotoxin ImI, methyllycaconitine, or hexamethonium. Aplysia transcriptome analysis followed by PCR yielded 20 full-length potential nicotinic receptor subunits. Sixteen of these were predicted to be cation selective, and real-time PCR suggested that 15 of the 16 subunits were expressed to varying degrees in the bag cell neurons. The acetylcholine-induced current, but not the nicotine current, was reduced by double-strand RNA treatment targeted to both subunits ApAChR-C and -E. Conversely, the nicotine-evoked current, but not the acetylcholine current, was lessened by targeting both subunits ApAChR-H and -P. To the best of our knowledge, this is the first report suggesting that a nicotinic receptor is not gated by acetylcholine. Separate receptors may serve as a means to differentially trigger plasticity or safeguard propagation by assuring that only acetylcholine, the endogenous agonist, initiates large enough responses to trigger reproduction. [ABSTRACT FROM AUTHOR]- Published
- 2014
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43. Separate Ca2+ Sources Are Buffered by Distinct Ca2+ Handling Systems in Aplysia Neuroendocrine Cells.
- Author
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Groten, Christopher J., Rebane, Jonathan T., Blohm, Gunnar, and Magoski, Neil S.
- Subjects
APLYSIA californica ,NEUROPLASTICITY ,NEURONS ,NEUROENDOCRINE cells ,MITOCHONDRIAL pathology ,SARCOPLASMIC reticulum - Abstract
Although the contribution of Ca
2+ buffering systems can vary between neuronal types and cellular compartments, it is unknown whether distinct Ca2+ sources within a neuron have different buffers. As individual Ca2+ sources can have separate functions, we propose that each is handled by unique systems. Using Aplysia californica bag cell neurons, which initiate reproduction through an afterdischarge involving multiple Ca2+ -dependent processes, we investigated the role of endoplasmic reticulum (ER) and mitochondrial sequestration, as well as extrusion via the plasma membrane Ca2+ -ATPase (PMCA) and Na+ /Ca2+ exchanger, to the clearance of voltage-gated Ca2+ influx, Ca2+ -induced Ca2+ -release (CICR), and store-operated Ca2+ influx. Cultured bag cell neurons were filled with the Ca2+ indicator, fura-PE3, to image Ca2+ under whole-cell voltage clamp. A 5 Hz, 1 min train of depolarizing voltage steps elicited voltage-gated Ca2+ influx followed by EGTA-sensitive CICR from the mitochondria. A compartment model of Ca2+ indicated the effect of EGTA on CICR was due to buffering of released mitochondrial Ca2+ rather than uptake competition. Removal of voltage-gated Ca2+ influx was dominated by the mitochondria and PMCA, with no contribution from the Na+ /Ca2+ exchanger or sarcoplasmic/endoplasmic Ca2+ -ATPase (SERCA). In contrast, CICR recovery was slowed by eliminating the Na+ /Ca2+ exchanger and PMCA. Last, store-operated influx, evoked by ER depletion, was removed by the SERCA and depended on the mitochondrial membrane potential. Our results demonstrate that distinct buffering systems are dedicated to particular Ca2+ sources. In general, this may represent a means to differentially regulate Ca2+ -dependent processes, and for Aplysia, influence how reproductive behavior is triggered. [ABSTRACT FROM AUTHOR]- Published
- 2013
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44. Acetylcholine-evoked afterdischarge in Aplysia bag cell neurons.
- Author
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White, Sean H.
- Subjects
ACETYLCHOLINE ,APLYSIA ,NEURONS ,PEPTIDE hormones ,OVULATION ,SYNAPSES ,MECAMYLAMINE - Abstract
A brief synaptic input to the bag cell neurons of Aplysia evokes a lengthy afterdischarge and the secretion of peptide hormones that trigger ovulation. The input transmitter is unknown, although prior work has shown that afterdischarges are prevented by strychnine. Because molluscan excitatory cholinergic synapses are blocked by strychnine, we tested the hypothesis that acetylcholine acts on an ionotropic receptor to initiate the afterdischarge. In cultured bag cell neurons, acetylcholine induced a short burst of action potentials followed by either return to near baseline or, like a true afterdischarge, transition to continuous firing. The current underlying the acetylcholine-induced depolarization was dose dependent, associated with increased membrane conductance, and sensitive to the nicotinic antagonists hexamethonium, mecamylamine, and α-conotoxin ImI. Whereas nicotine, choline, carbachol, and glycine did not mimic acetylcholine, tetramethylammonium did produce a similar current. Consistent with an ionotropic receptor, the response was not altered by intracellular dialysis with the G protein blocker guanosine 5'-(β-thio)diphosphate. Recording from the intact bag cell neuron cluster showed acetylcholine to evoke prominent depolarization, which often led to extended bursting, but only in the presence of the acetylcholinesterase inhibitor neostigmine. Extracellular recording confirmed that exogenous acetylcholine caused genuine afterdischarges, which, as per those generated synaptically, rendered the cluster refractory to further stimulation. Finally, treatment with a combination of mecamylamine and α-conotoxin ImI blocked synaptically induced afterdischarges in the intact bag cell neuron cluster. Acetylcholine appears to elicit the afterdischarge through an ionotropic receptor. This represents an expedient means for transient stimulation to elicit prolonged firing in the absence of ongoing synaptic input. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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45. Mitochondrial Ca2+Activates a Cation Current in Aplysia Bag Cell Neurons.
- Published
- 2010
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46. Persistent Ca2+ Current Contributes to a Prolonged Depolarization in Aplysia Bag Cell Neurons.
- Author
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Tam, Alan K. H., Geiger, Julia E., Hung, Anne Y., Groten, Chris J., and Magoski, Neil S.
- Abstract
Neurons may initiate behavior or store information by translating prior activity into a lengthy change in excitability. For example, brief input to the bag cell neurons of Aplysia results in an approximate 30-min afterdischarge that induces reproduction. Similarly, momentary stimulation of cultured bag cells neurons evokes a prolonged depolarization lasting many minutes. Contributing to this is a voltage-independent cation current activated by Ca(2
+ ) entering during the stimulus. However, the cation current is relatively short-lived, and we hypothesized that a second, voltage-dependent persistent current sustains the prolonged depolarization. In bag cell neurons, the inward voltage-dependent current is carried by Ca(2+ ); thus we tested for persistent Ca(2+ ) current in primary culture under voltage clamp. The observed current activated between -40 and -50 mV exhibited a very slow decay, presented a similar magnitude regardless of stimulus duration (10-60 s), and, like the rapid Ca(2+ ) current, was enhanced when Ba(2+ ) was the permeant ion. The rapid and persistent Ca(2+ ) current, but not the cation current, were Ni(2+ ) sensitive. Consistent with the persistent current contributing to the response, Ni(2+ ) reduced the amplitude of a prolonged depolarization evoked under current clamp. Finally, protein kinase C activation enhanced the rapid and persistent Ca(2+ ) current as well as increased the prolonged depolarization when elicited by an action potential-independent stimulus. Thus the prolonged depolarization arises from Ca(2+ ) influx triggering a cation current, followed by voltage-dependent activation of a persistent Ca(2+ ) current and is subject to modulation. Such synergy between currents may represent a common means of achieving activity-dependent changes to excitability. [ABSTRACT FROM AUTHOR]- Published
- 2009
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47. Neuronal Mitochondrial Calcium Uniporter (MCU) Deficiency Is Neuroprotective in Hyperexcitability by Modulation of Metabolic Pathways and ROS Balance
- Author
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Bierhansl, Laura, Gola, Lukas, Narayanan, Venu, Dik, Andre, Meuth, Sven G., Wiendl, Heinz, and Kovac, Stjepana
- Published
- 2024
- Full Text
- View/download PDF
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