22 results on '"Gennarino, Vincenzo A"'
Search Results
2. Correction: Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis
- Author
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Uyhazi, Katherine E., Yang, Yiying, Liu, Na, Qi, Hongying, Huang, Xiao A., Mak, Winifred, Weatherbee, Scott D., de Prisco, Nicola, Gennarino, Vincenzo A., Song, Xiaoling, and Lin, Haifan
- Published
- 2020
3. Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis
- Author
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Uyhazi, Katherine E., Yang, Yiying, Liu, Na, Qi, Hongying, Huang, Xiao A., Mak, Winifred, Weatherbee, Scott D., de Prisco, Nicola, Gennarino, Vincenzo A., Song, Xiaoling, and Lin, Haifan
- Published
- 2020
4. MR Imaging in Ataxias: Consensus Recommendations by the Ataxia Global Initiative Working Group on MRI Biomarkers.
- Author
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Öz, Gülin, Cocozza, Sirio, Henry, Pierre-Gilles, Lenglet, Christophe, Deistung, Andreas, Faber, Jennifer, Schwarz, Adam J., Timmann, Dagmar, Van Dijk, Koene R. A., Harding, Ian H., Adarmes-Gomez, Astrid, Thieme, Andreas, Reetz, Kathrin, Rylski, Marcin, Rezende, Thiago JR, Gennarino, Vincenzo A., Ratai, Eva-Maria, Mariotti, Caterina, Nigri, Anna, and Nanetti, Lorenzo
- Subjects
MAGNETIC resonance imaging ,DIFFUSION magnetic resonance imaging ,NUCLEAR magnetic resonance spectroscopy ,FUNCTIONAL magnetic resonance imaging ,ATAXIA - Abstract
With many viable strategies in the therapeutic pipeline, upcoming clinical trials in hereditary and sporadic degenerative ataxias will benefit from non-invasive MRI biomarkers for patient stratification and the evaluation of therapies. The MRI Biomarkers Working Group of the Ataxia Global Initiative therefore devised guidelines to facilitate harmonized MRI data acquisition in clinical research and trials in ataxias. Recommendations are provided for a basic structural MRI protocol that can be used for clinical care and for an advanced multi-modal MRI protocol relevant for research and trial settings. The advanced protocol consists of modalities with demonstrated utility for tracking brain changes in degenerative ataxias and includes structural MRI, magnetic resonance spectroscopy, diffusion MRI, quantitative susceptibility mapping, and resting-state functional MRI. Acceptable ranges of acquisition parameters are provided to accommodate diverse scanner hardware in research and clinical contexts while maintaining a minimum standard of data quality. Important technical considerations in setting up an advanced multi-modal protocol are outlined, including the order of pulse sequences, and example software packages commonly used for data analysis are provided. Outcome measures most relevant for ataxias are highlighted with use cases from recent ataxia literature. Finally, to facilitate access to the recommendations by the ataxia clinical and research community, examples of datasets collected with the recommended parameters are provided and platform-specific protocols are shared via the Open Science Framework. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Dosage sensitivity to Pumilio1 variants in the mouse brain reflects distinct molecular mechanisms.
- Author
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Botta, Salvatore, de Prisco, Nicola, Chemiakine, Alexei, Brandt, Vicky, Cabaj, Maximilian, Patel, Purvi, Doron‐Mandel, Ella, Treadway, Colton J, Jovanovic, Marko, Brown, Nicholas G, Soni, Rajesh K, and Gennarino, Vincenzo A
- Subjects
RNA-binding proteins ,GENETIC variation ,DEVELOPMENTAL delay ,PROTEIN expression ,MICE - Abstract
Different mutations in the RNA‐binding protein Pumilio1 (PUM1) cause divergent phenotypes whose severity tracks with dosage: a mutation that reduces PUM1 levels by 25% causes late‐onset ataxia, whereas haploinsufficiency causes developmental delay and seizures. Yet PUM1 targets are derepressed to equal degrees in both cases, and the more severe mutation does not hinder PUM1's RNA‐binding ability. We therefore considered the possibility that the severe mutation might disrupt PUM1 interactions, and identified PUM1 interactors in the murine brain. We find that mild PUM1 loss derepresses PUM1‐specific targets, but the severe mutation disrupts interactions with several RNA‐binding proteins and the regulation of their targets. In patient‐derived cell lines, restoring PUM1 levels restores these interactors and their targets to normal levels. Our results demonstrate that dosage sensitivity does not always signify a linear relationship with protein abundance but can involve distinct mechanisms. We propose that to understand the functions of RNA‐binding proteins in a physiological context will require studying their interactions as well as their targets. Synopsis: Different variants in a gene can alter protein expression to different degrees, producing milder or more severe phenotypes. Here, two variants of the RNA‐binding protein PUM1 are shown to act via distinct molecular mechanisms: deregulation of direct targets or disruption of PUM1 interactors and their targets. Mild PUM1 loss de‐represses PUM1's direct targets.PUM1 haploinsufficiency disrupts native interactions and downstream targets.Dosage sensitivity can reflect distinct mechanisms rather than linear change in severity.To understand RNA‐binding proteins requires investigation of both their targets and interactors. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
6. Correction to: MR Imaging in Ataxias: Consensus Recommendations by the Ataxia Global Initiative Working Group on MRI Biomarkers.
- Author
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Öz, Gülin, Cocozza, Sirio, Henry, Pierre-Gilles, Lenglet, Christophe, Deistung, Andreas, Faber, Jennifer, Schwarz, Adam J., Timmann, Dagmar, Van Dijk, Koene R. A., Harding, Ian H., Adarmes-Gómez, Astrid, Thieme, Andreas, Reetz, Kathrin, Rylski, Marcin, Rezende, Thiago JR, Gennarino, Vincenzo A., Ratai, Eva-Maria, Mariotti, Caterina, Nigri, Anna, and Nanetti, Lorenzo
- Subjects
MAGNETIC resonance imaging ,ATAXIA ,ACADEMIC medical centers ,BIOMARKERS - Abstract
This document is a correction notice for an article titled "MR Imaging in Ataxias: Consensus Recommendations by the Ataxia Global Initiative Working Group on MRI Biomarkers" published in the journal Cerebellum. The original article did not properly capture the list of collaborating authors in PubMed, but this has been corrected in both the original article and the PubMed listing. The correction notice includes the names and affiliations of the collaborating authors, who are members of the AGI MR Biomarkers Study Group. The correction notice also includes a note from the publisher, Springer Nature, stating their neutrality regarding jurisdictional claims and institutional affiliations. [Extracted from the article]
- Published
- 2024
- Full Text
- View/download PDF
7. Fragile X-like behaviors and abnormal cortical dendritic spines in Cytoplasmic FMR1-interacting protein 2-mutant mice
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Han, Kihoon, Chen, Hogmei, Gennarino, Vincenzo A., Richman, Ronald, Lu, Hui-Chen, and Zoghbi, Huda Y.
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- 2015
- Full Text
- View/download PDF
8. A gene network regulating lysosomal biogenesis and function
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Sardiello, Marco, Palmieri, Michela, di Ronza, Alberto, Medina, Diego Luis, Valenza, Marta, Gennarino, Vincenzo Alessandro, Di Malta, Chiara, Donaudy, Francesca, Embrione, Valerio, Polishchuk, Roman S., Banfi, Sandro, Parenti, Giancarlo, Cattaneo, Elena, and Ballabio, Andrea
- Subjects
Lysosomes -- Properties ,Gene expression -- Research ,DNA binding proteins -- Properties ,Huntington's chorea -- Development and progression ,Science and technology - Abstract
Lysosomes are organelies central to degradation and recycling processes in animal cells. Whether lysosomal activity is coordinated to respond to cellular needs remains unclear. We found that most lysosomal genes exhibit coordinated transcriptional behavior and are regulated by the transcription factor EB (TFEB). Under aberrant lysosomal storage conditions, TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes. TFEB overexpression in cultured cells induced lysosomal biogenesis and increased the degradation of complex molecules, such as glycosaminoglycans and the pathogenic protein that causes Huntington's disease. Thus, a genetic program controls lysosomal biogenesis and function, providing a potential therapeutic target to enhance cellular clearing in lysosomal storage disorders and neurodegenerative diseases.
- Published
- 2009
9. UTRdb and UTRsite (RELEASE 2010): a collection of sequences and regulatory motifs of the untranslated regions of eukaryotic mRNAs
- Author
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Grillo, Giorgio, Turi, Antonio, Licciulli, Flavio, Mignone, Flavio, Liuni, Sabino, Banfi, Sandro, Gennarino, Vincenzo Alessandro, Horner, David S., Pavesi, Giulio, Picardi, Ernesto, and Pesole, Graziano
- Published
- 2010
- Full Text
- View/download PDF
10. Modulation of Pancreatic Neuroendocrine Neoplastic Cell Fate by Autophagy-Mediated Death.
- Author
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Matrood, Sami, de Prisco, Nicola, Wissniowski, Thaddeus T., Wiese, Dominik, Jabari, Samir, Griesmann, Heidi, Wanzel, Michael, Stiewe, Thorsten, Neureiter, Daniel, Klieser, Eckhard, Mintziras, Ioannis, Buchholz, Malte, Bartsch, Detlef K., Gennarino, Vincenzo A., and Di Fazio, Pietro
- Subjects
NEUROENDOCRINE cells ,CELL death ,ISLANDS of Langerhans ,MEMBRANE proteins ,CANCER cells ,HISTONE deacetylase - Abstract
Introduction: Autophagic cell death in cancer cells can be mediated by inhibition of deacetylases. Although extensive studies have focused on the autophagic process in cancer, little is known about the role of autophagy in degrading cytosolic and nuclear components of pancreatic neuroendocrine neoplastic (pNEN) cells leading to cell death, thus improving the therapy of patients affected by pNEN. Methods: 2D and 3D human pNEN and pancreatic stellate cells were treated with panobinostat and bafilomycin. Autophagy markers were detected by RT-qPCR, immunofluorescence, and Western blot. Autophagosomes were detected by electron microscopy and their maturation by real-time fluorescence of LC3B stable transfected cells. ChIP was performed at the cAMP responsive element. Immunofluorescence was performed in murine pancreatic tissue. Results: We observed that pan-deacetylase inhibitor panobinostat treatment causes autophagic cell death in pNEN cells. We also found that although AMPK-α phosphorylation is counterbalanced by phosphorylated AKT, it is not capable to inhibiting autophagic cell death. However, the binding activity of the cAMP responsive element is prompted by panobinostat. Although autophagy inhibition prevented autophagosome synthesis, maturation, and cell death, panobinostat treatment induced the accumulation of mature autophagosomes in the cytosol and the nucleus, leading to disruption of the organelles, cellular digestion, and decay. Observation of autophagosome membrane proteins Beclin1 and LC3B aggregation in murine pancreatic islets indicates that autophagy restoration may also lead to autophagosome aggregation in murine insulinoma cells. A basal low expression of autophagy markers was detectable in patients affected by pNEN, and, interestingly, the expression of these markers was significantly lower in metastatic pNEN. Discussion/Conclusion: Our study highlights that the autophagy functional restoration and prolongation of this catabolic process, mediated by inhibition of deacetylase, is responsible for the reduction of pNEN cells. Prompting of autophagy cell death could be a promising strategy for the therapy of pNEN. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
11. miRNeye: a microRNA expression atlas of the mouse eye
- Author
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Karali, Marianthi, Peluso, Ivana, Gennarino, Vincenzo A, Bilio, Marchesa, Verde, Roberta, Lago, Giampiero, Dollé, Pascal, and Banfi, Sandro
- Published
- 2010
- Full Text
- View/download PDF
12. miRNeye: a microRNA expression atlas of the mouse eye
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Verde Roberta, Bilio Marchesa, Gennarino Vincenzo A, Peluso Ivana, Karali Marianthi, Lago Giampiero, Dollé Pascal, and Banfi Sandro
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. Results In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. Conclusions This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.
- Published
- 2010
- Full Text
- View/download PDF
13. Promiscuity of enhancer, coding and non-coding transcription functions in ultraconserved elements
- Author
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Sanges Remo, Petrera Francesca, Gennarino Vincenzo A, Licastro Danilo, Banfi Sandro, and Stupka Elia
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Ultraconserved elements (UCEs) are highly constrained elements of mammalian genomes, whose functional role has not been completely elucidated yet. Previous studies have shown that some of them act as enhancers in mouse, while some others are expressed in both normal and cancer-derived human tissues. Only one UCE element so far was shown to present these two functions concomitantly, as had been observed in other isolated instances of single, non ultraconserved enhancer elements. Results We used a custom microarray to assess the levels of UCE transcription during mouse development and integrated these data with published microarray and next-generation sequencing datasets as well as with newly produced PCR validation experiments. We show that a large fraction of non-exonic UCEs is transcribed across all developmental stages examined from only one DNA strand. Although the nature of these transcripts remains a mistery, our meta-analysis of RNA-Seq datasets indicates that they are unlikely to be short RNAs and that some of them might encode nuclear transcripts. In the majority of cases this function overlaps with the already established enhancer function of these elements during mouse development. Utilizing several next-generation sequencing datasets, we were further able to show that the level of expression observed in non-exonic UCEs is significantly higher than in random regions of the genome and that this is also seen in other regions which act as enhancers. Conclusion Our data shows that the concurrent presence of enhancer and transcript function in non-exonic UCE elements is more widespread than previously shown. Moreover through our own experiments as well as the use of next-generation sequencing datasets, we were able to show that the RNAs encoded by non-exonic UCEs are likely to be long RNAs transcribed from only one DNA strand.
- Published
- 2010
- Full Text
- View/download PDF
14. NUDT21-spanning CNVs lead to neuropsychiatric disease and altered MeCP2 abundance via alternative polyadenylation.
- Author
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Gennarino, Vincenzo A., Alcott, Callison E., Chun-An Chen, Chaudhury, Arindam, Gillentine, Madelyn A., Rosenfeld, Jill A., Parikh, Sumit, Wheless, James W., Roeder, Elizabeth R., Horovitz, Dafne DG, Roney, Erin K., Smith, Janice L., Cheung, Sau W., Wei Li, Neilson, Joel R., Schaaf, Christian P., and Zoghbi, Huda Y.
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DNA copy number variations , *NEUROBEHAVIORAL disorders , *CARRIER proteins , *GENETICS - Abstract
The article discusses a study which shows the role of copy-number variations spanning NUDT21 in the development of intellectual disorder and neuropsychiatric disease and the alteration of methyl CpG binding protein 2 (MeCP2) through alternative polyadenylation.
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- 2015
- Full Text
- View/download PDF
15. Fragile X-like behaviors and abnormal cortical dendritic spines Cytoplasmic FMR1-interacting protein 2-mutant mice.
- Author
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Kihoon Han, Hogmei Chen, Gennarino, Vincenzo A., Richman, Ronald, Hui-Chen Lu, and Zoghbi, Huda Y.
- Published
- 2015
- Full Text
- View/download PDF
16. Pumilio1 Haploinsufficiency Leads to SCA1-like Neurodegeneration by Increasing Wild-Type Ataxin1 Levels.
- Author
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Gennarino, Vincenzo A., Singh, Ravi K., White, Joshua J., De Maio, Antonia, Han, Kihoon, Kim, Ji-Yoen, Jafar-Nejad, Paymaan, di Ronza, Alberto, Kang, Hyojin, Sayegh, Layal S., Cooper, Thomas A., Orr, Harry T., Sillitoe, Roy V., and Zoghbi, Huda Y.
- Subjects
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SPINOCEREBELLAR ataxia , *NEURODEGENERATION , *ATAXIN-1 , *MOTOR ability , *MUTANT proteins , *BIOACCUMULATION , *CARRIER proteins - Abstract
Summary Spinocerebellar ataxia type 1 (SCA1) is a paradigmatic neurodegenerative proteinopathy, in which a mutant protein (in this case, ATAXIN1) accumulates in neurons and exerts toxicity; in SCA1, this process causes progressive deterioration of motor coordination. Seeking to understand how post-translational modification of ATAXIN1 levels influences disease, we discovered that the RNA-binding protein PUMILIO1 (PUM1) not only directly regulates ATAXIN1 but also plays an unexpectedly important role in neuronal function. Loss of Pum1 caused progressive motor dysfunction and SCA1-like neurodegeneration with motor impairment, primarily by increasing Ataxin1 levels. Breeding Pum1 +/− mice to SCA1 mice ( Atxn1 154Q/+ ) exacerbated disease progression, whereas breeding them to Atxn1 +/− mice normalized Ataxin1 levels and largely rescued the Pum1 +/− phenotype. Thus, both increased wild-type ATAXIN1 levels and PUM1 haploinsufficiency could contribute to human neurodegeneration. These results demonstrate the importance of studying post-transcriptional regulation of disease-driving proteins to reveal factors underlying neurodegenerative disease. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
17. Human-specific regulation of MeCP2 levels in fetal brains by microRNA miR-483-5p.
- Author
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Kihoon Han, Gennarino, Vincenzo Alessandro, Yoontae Lee, Kaifang Pang, Hashimoto-Torii, Kazue, Sanaa Choufani, Raju, Chandrasekhar S., Oldham, Michael C., Weksberg, Rosanna, Rakic, Pasko, Zhandong Liu, and Zoghbi, Huda Y.
- Subjects
- *
FETAL brain , *MICRORNA , *EPIGENETICS , *CARRIER proteins - Abstract
Proper neurological function in humans requires precise control of levels of the epigenetic regulator methyl CpG-binding protein 2 (MeCP2). MeCP2 protein levels are low in fetal brains, where the predominant MECP2 transcripts have an unusually long 3' untranslated region (UTR). Here, we show that miR-483-5p, an intragenic microRNA of the imprinted IGF2, regulates MeCP2 levels through a human-specific binding site in the MECP2 long 3' UTR. We demonstrate the inverse correlation of miR-483-5p and MeCP2 levels in developing human brains and fibroblasts from Beckwith-Wiedemann syndrome patients. Importantly, expression of miR-483-5p rescues abnormal dendritic spine phenotype of neurons overexpressing human MeCP2. In addition, miR-483-5p modulates the levels of proteins of the MeCP2-interacting corepressor complexes, including HDAC4 and TBL1X. These data provide insight into the role of miR-483-5p in regulating the levels of MeCP2 and interacting proteins during human fetal development. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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- View/download PDF
18. Identification of microRNA-regulated gene networks by expression analysis of target genes.
- Author
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Gennarino, Vincenzo Alessandro, D'Angelo, Giovanni, Dharmalingam, Gopuraja, Fernandez, Serena, Russolillo, Giorgio, Sanges, Remo, Mutarelli, Margherita, Belcastro, Vincenzo, Ballabio, Andrea, Verde, Pasquale, Sardiello, Marco, and Banfi, Sandro
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- *
MICRORNA , *GENE regulatory networks , *GENE expression , *TRANSCRIPTION factors , *EUKARYOTIC cells - Abstract
MicroRNAs (miRNAs) and transcription factors control eukaryotic cell proliferation, differentiation, and metabolism through their specific gene regulatory networks. However, differently from transcription factors, our understanding of the processes regulated by miRNAs is currently limited. Here, we introduce gene network analysis as a new means for gaining insight into miRNA biology. A systematic analysis of all human miRNAs based on Co-expression Meta-analysis of miRNA Targets (CoMeTa) assigns high-resolution biological functions to miRNAs and provides a comprehensive, genome-scale analysis of human miRNA regulatory networks. Moreover, gene cotargeting analyses show that miRNAs synergistically regulate cohorts of genes that participate in similar processes. We experimentally validate the CoMeTa procedure through focusing on three poorly characterized miRNAs, miR-519d/190/340, which CoMeTa predicts to be associated with the TGFβ pathway. Using lung adenocarcinoma A549 cells as a model system, we show that miR-519d and miR-190 inhibit, while miR-340 enhances TGFβ signaling and its effects on cell proliferation, morphology, and scattering. Based on these findings, we formalize and propose co-expression analysis as a general paradigm for second-generation procedures to recognize bona fide targets and infer biological roles and network communities of miRNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
19. HOCTAR database: A unique resource for microRNA target prediction
- Author
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Gennarino, Vincenzo Alessandro, Sardiello, Marco, Mutarelli, Margherita, Dharmalingam, Gopuraja, Maselli, Vincenza, Lago, Giampiero, and Banfi, Sandro
- Subjects
- *
NON-coding RNA , *GENE expression , *MESSENGER RNA , *ONTOLOGY , *MEMBRANE proteins , *ONCOGENES , *GENETIC transcription , *FORECASTING - Abstract
Abstract: microRNAs (miRNAs) are the most abundant class of small RNAs in mammals. They play an important role in regulation of gene expression by inducing mRNA cleavage or translational inhibition. Each miRNA targets an average of 100–200 genes by binding, preferentially, to their 3′ UTRs by means of partial sequence complementarity. Most miRNAs are localized within transcriptional units, termed host genes, and show similar expression behavior with respect to their corresponding host genes. Considering the impact of miRNA in the regulation of gene expression and their involvement in a growing number of human disorders, it is vital to develop sensitive computational approaches able to identify miRNA target genes. The HOCTAR database (db) is a publicly available resource collecting ranked list of predicted target genes for 290 intragenic miRNAs annotated in human. HOCTARdb is a unique resource that integrates miRNA target prediction genes and transcriptomic data to score putative miRNA targets looking at the expression behavior of their host genes. We demonstrated, by testing 135 known validated target genes (either at the translational or transcriptional level) for different miRNAs, that the miRNA target prediction lists present in HOCTARdb are highly reliable. Moreover, HOCTARdb associates biological roles to each miRNA-controlled transcriptional network by means of Gene Ontology analysis. This information is easily accessible through a user-friendly query page. The HOCTARdb is available at http://hoctar.tigem.it/. We believe that a detailed relationship between miRNAs and their target genes and a constant update of the information contained in HOCTARdb will provide an extremely valuable resource to assist the researcher in the discovery of miRNA target genes. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
20. Promiscuity of enhancer, coding and non-codingtranscription functions in ultraconservedelements.
- Author
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Licastro, Danilo, Gennarino, Vincenzo A., Petrera, Francesca, Sanges, Remo, Banfi, Sandro, and Stupka, Elia
- Subjects
- *
GENOMES , *GENETICS , *META-analysis , *CANCER education , *GENES - Abstract
Background: Ultraconserved elements (UCEs) are highly constrained elements of mammalian genomes, whose functional role has not been completely elucidated yet. Previous studies have shown that some of them act as enhancers in mouse, while some others are expressed in both normal and cancer-derived human tissues. Only one UCE element so far was shown to present these two functions concomitantly, as had been observed in other isolated instances of single, non ultraconserved enhancer elements. Results: We used a custom microarray to assess the levels of UCE transcription during mouse development and integrated these data with published microarray and next-generation sequencing datasets as well as with newly produced PCR validation experiments. We show that a large fraction of non-exonic UCEs is transcribed across all developmental stages examined from only one DNA strand. Although the nature of these transcripts remains a mistery, our meta-analysis of RNA-Seq datasets indicates that they are unlikely to be short RNAs and that some of them might encode nuclear transcripts. In the majority of cases this function overlaps with the already established enhancer function of these elements during mouse development. Utilizing several next-generation sequencing datasets, we were further able to show that the level of expression observed in non-exonic UCEs is significantly higher than in random regions of the genome and that this is also seen in other regions which act as enhancers. Conclusion: Our data shows that the concurrent presence of enhancer and transcript function in non-exonic UCE elements is more widespread than previously shown. Moreover through our own experiments as well as the use of next-generation sequencing datasets, we were able to show that the RNAs encoded by non-exonic UCEs are likely to be long RNAs transcribed from only one DNA strand. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
21. miRNeye: a microRNA expression atlas of themouse eye.
- Author
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Karali, Marianthi, Peluso, Ivana, Gennarino, Vincenzo A., Bilio, Marchesa, Verde, Roberta, Lago, Giampiero, Dollé, Pascal, and Banfi, Sandro
- Subjects
CRYOBIOLOGY ,VITAMIN A ,RETINAL (Visual pigment) ,POSTERIOR segment (Eye) ,WEB databases - Abstract
Background: MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. Results: In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a highresolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. Conclusions: This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
22. RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins.
- Author
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De Maio, Antonia, Yalamanchili, Hari Krishna, Adamski, Carolyn J., Gennarino, Vincenzo A., Liu, Zhandong, Qin, Jun, Jung, Sung Y., Richman, Ronald, Orr, Harry, and Zoghbi, Huda Y.
- Abstract
Summary RNA splicing entails the coordinated interaction of more than 150 proteins in the spliceosome, one of the most complex of the cell's molecular machines. We previously discovered that the RNA-binding motif protein 17 (RBM17), a component of the spliceosome, is essential for survival and cell maintenance. Here, we find that it interacts with the spliceosomal factors U2SURP and CHERP and that they reciprocally regulate each other's stability, both in mouse and in human cells. Individual knockdown of each of the three proteins induces overlapping changes in splicing and gene expression of transcripts enriched for RNA-processing factors. Our results elucidate the function of RBM17, U2SURP, and CHERP and link the activity of the spliceosome to the regulation of downstream RNA-binding proteins. These data support the hypothesis that, beyond driving constitutive splicing, spliceosomal factors can regulate alternative splicing of specific targets. Graphical Abstract Highlights • RBM17 is essential at any stage of life • RBM17, U2SURP, and CHERP regulate reciprocal protein stability • Knockdown of RBM17 , U2SURP , or CHERP does not inhibit splicing • RBM17, U2SURP, and CHERP together regulate a specific set of transcripts De Maio et al. find that the splicing factor RBM17 establishes a physical and functional relation with U2SURP and CHERP. Knockdown of these U2 snRNP-associated spliceosomal components reveals their synergistic activity toward regulation of a given set of transcripts rather than a more predictable transcriptome-wide inhibition of splicing. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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