1. Correlation of Glioma Cell Regression with Inhibition of Insulin-Like Growth Factor 1 and Insulin-Like Growth Factor-Binding Protein-2 Expression
- Author
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Stanley Samuels, Ma J, Catanese Vm, Edwin H. Kolodny, Zhao-Hui Wang, Zeng Bj, and Gama Sosa Ma
- Subjects
medicine.medical_specialty ,Cell Survival ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Genetic Vectors ,Insulin-like growth factor-binding protein ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Insulin-like growth factor ,Endocrinology ,Retrovirus ,Growth factor receptor ,Glioma ,Internal medicine ,Tumor Cells, Cultured ,medicine ,Animals ,RNA, Antisense ,Growth factor receptor inhibitor ,Insulin-Like Growth Factor I ,Cell Line, Transformed ,biology ,Endocrine and Autonomic Systems ,Growth factor ,DNA, Neoplasm ,biology.organism_classification ,medicine.disease ,Rats ,Antisense RNA ,Insulin-Like Growth Factor Binding Protein 2 ,Phenotype ,Retroviridae ,Culture Media, Conditioned ,biology.protein ,Cancer research ,Cell Division - Abstract
To explore the antitumor effect of insulin-like growth factor 1 (IGF-I) antisense RNA and the interaction of IGF-I with insulin-like growth factor-binding proteins (IGFBPs) in glioma cells, a recombinant retrovirus expressing IGF-I antisense RNA was constructed and introduced into C6 glioma cells. IGF-I antisense RNA reverses the transformed phenotype in glioma cells and inhibits glioma cell growth by blocking overexpression of endogenous IGF-I. Expression of IGFBP-2 is increased in glioma cells as compared with normal adult glial cells. IGF-I antisense RNA also inhibits expression of IGFBP-2 in glioma cells, but does not influence expression of the other IGFBPs. Although IGFBP-2 in conditioned medium from wild-type C6 cell cultures itself does not directly influence glioma cell growth, it synergistically enhances exogenous IGF-I-mediated DNA synthesis in IGF-I-negative C6 cells. These findings indicate the inhibitory effect of IGF-I antisense RNA on growth and development of glioma cells. IGF-I-dependent glioma cell growth may, in some circumstances, require IGFBP-2 as a cofactor. The antitumor effect of IGF-I antisense RNA is also associated with inhibition of IGFBP-2 expression.
- Published
- 1997
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