18 results on '"Folgiero, Valentina"'
Search Results
2. GMP-manufactured CRISPR/Cas9 technology as an advantageous tool to support cancer immunotherapy.
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Caforio, M, Iacovelli, S, Quintarelli, C, Locatelli, F, and Folgiero, Valentina
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CRISPRS ,IMMUNOTHERAPY ,CANCER treatment ,COMBINATION drug therapy ,GENE therapy - Abstract
Background: CRISPR/Cas9 system to treat human-related diseases has achieved significant results and, even if its potential application in cancer research is improving, the application of this approach in clinical practice is still a nascent technology. Main body: CRISPR/Cas9 technology is not yet used as a single therapy to treat tumors but it can be combined with traditional treatment strategies to provide personalized gene therapy for patients. The combination with chemotherapy, radiation and immunotherapy has been proven to be a powerful means of screening, identifying, validating and correcting tumor targets. Recently, CRISPR/Cas9 technology and CAR T-cell therapies have been integrated to open novel opportunities for the production of more efficient CAR T-cells for all patients. GMP-compatible equipment and reagents are already available for several clinical-grade systems at present, creating the basis and framework for the accelerated development of novel treatment methods. Conclusion: Here we will provide a comprehensive collection of the actual GMP-grade CRISPR/Cas9-mediated approaches used to support cancer therapy highlighting how this technology is opening new opportunities for treating tumors. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Manipulating the tumor immune microenvironment to improve cancer immunotherapy: IGF1R, a promising target.
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Pellegrino, Marsha, Secli, Valerio, D’Amico, Silvia, Petrilli, Lucia Lisa, Caforio, Matteo, Folgiero, Valentina, Tumino, Nicola, Vacca, Paola, Vinci, Maria, Fruci, Doriana, and de Billy, Emmanuel
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TUMOR microenvironment ,INSULIN-like growth factor receptors ,SOMATOMEDIN C ,IMMUNOTHERAPY ,CELL physiology - Abstract
Cancer immunotherapy has made impressive advances in improving the outcome of patients affected by malignant diseases. Nonetheless, some limitations still need to be tackled to more efficiently and safely treat patients, in particular for those affected by solid tumors. One of the limitations is related to the immunosuppressive tumor microenvironment (TME), which impairs antitumor immunity. Efforts to identify targets able to turn the TME into a milieu more auspicious to current immuno-oncotherapy is a real challenge due to the high redundancy of the mechanisms involved. However, the insulin-like growth factor 1 receptor (IGF1R), an attractive drug target for cancer therapy, is emerging as an important immunomodulator and regulator of key immune cell functions. Here, after briefly summarizing the IGF1R signaling pathway in cancer, we review its role in regulating immune cells function and activity, and discuss IGF1R as a promising target to improve anti-cancer immunotherapy. [ABSTRACT FROM AUTHOR]
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- 2024
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4. AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation.
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Caforio, Matteo, Tumino, Nicola, Sorino, Cristina, Manni, Isabella, Di Giovenale, Stefano, Piaggio, Giulia, Iezzi, Simona, Strimpakos, Georgios, Mattei, Elisabetta, Moretta, Lorenzo, Fanciulli, M., Vacca, Paola, Locatelli, Franco, and Folgiero, Valentina
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RNA polymerase II ,CARRIER proteins ,KILLER cells ,GENETIC overexpression ,CELL receptors - Abstract
Introduction: AATF/Che-1 over-expression in different tumors is well known and its effect on tumorigenicity is mainly due to its central role demonstrated in the oncogenic pathways of solid tumors, where it controls proliferation and viability. The effect exerted by tumors overexpressing Che-1 on the immune response has not yet been investigated. Methods: Starting from ChIP-sequencing data we confirmed Che-1 enrichment on Nectin-1 promoter. Several co-cultures experiments between NK-cells and tumor cells transduced by lentiviral vectors carrying Che-1-interfering sequence, analyzed by flow-cytometry have allowed a detailed characterization of NK receptors and tumor ligands expression. Results: Here, we show that Che-1 is able to modulate the expression of Nectin-1 ligand at the transcriptional level, leading to the impairment of killing activity of NK-cells. Nectin-1 down-modulation induces a modification in NK-cell ligands expression able to interact with activating receptors and to stimulate NK-cell function. In addition, NK-cells from Che-1 transgenic mice, confirming a reduced expression of activating receptors, exhibit impaired activation and a preferential immature status. Discussion: The critical equilibrium between NK-cell ligand expression on tumor cells and the interaction with NK cell receptors is affected by Che-1 over-expression and partially restored by Che-1 interference. The evidence of a new role for Che-1 as regulator of anti-tumor immunity supports the necessity to develop approaches able to target this molecule which shows a dual tumorigenic function as cancer promoter and immune response modulator. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Che‐1 is targeted by c‐Myc to sustain proliferation in pre‐B‐cell acute lymphoblastic leukemia
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Folgiero, Valentina, Sorino, Cristina, Pallocca, Matteo, De Nicola, Francesca, Goeman, Frauke, Bertaina, Valentina, Strocchio, Luisa, Romania, Paolo, Pitisci, Angela, Iezzi, Simona, Catena, Valeria, Bruno, Tiziana, Strimpakos, Georgios, Passananti, Claudio, Mattei, Elisabetta, Blandino, Giovanni, Locatelli, Franco, and Fanciulli, Maurizio
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- 2018
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6. Indoleamine 2,3-dioxygenase 1 (IDO1) activity correlates with immune system abnormalities in multiple myeloma
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Bonanno Giuseppina, Mariotti Andrea, Procoli Annabella, Folgiero Valentina, Natale Daniela, De Rosa Luca, Majolino Ignazio, Novarese Linda, Rocci Alberto, Gambella Manuela, Ciciarello Marilena, Scambia Giovanni, Palumbo Antonio, Locatelli Franco, De Cristofaro Raimondo, and Rutella Sergio
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Medicine - Abstract
Abstract Background Multiple myeloma (MM) is a plasma cell malignancy with a multifaceted immune dysfunction. Indoleamine 2,3-dioxygenase 1 (IDO1) degrades tryptophan into kynurenine (KYN), which inhibits effector T cells and promote regulatory T-cell (Treg) differentiation. It is presently unknown whether MM cells express IDO1 and whether IDO1 activity correlates with immune system impairment. Methods We investigated IDO1 expression in 25 consecutive patients with symptomatic MM and in 7 patients with either monoclonal gammopathy of unknown significance (MGUS; n=3) or smoldering MM (SMM; n=4). IDO1-driven tryptophan breakdown was correlated with the release of hepatocyte growth factor (HGF) and with the frequency of Treg cells and NY-ESO-1-specific CD8+ T cells. Results KYN was increased in 75% of patients with symptomatic MM and correlated with the expansion of CD4+CD25+FoxP3+ Treg cells and the contraction of NY-ESO-1-specific CD8+ T cells. In vitro, primary MM cells promoted the differentiation of allogeneic CD4+ T cells into bona fide CD4+CD25hiFoxP3hi Treg cells and suppressed IFN-γ/IL-2 secretion, while preserving IL-4 and IL-10 production. Both Treg expansion and inhibition of Th1 differentiation by MM cells were reverted, at least in part, by d,l-1-methyl-tryptophan, a chemical inhibitor of IDO. Notably, HGF levels were higher within the BM microenvironment of patients with IDO+ myeloma disease compared with patients having IDO- MM. Mechanistically, the antagonism of MET receptor for HGF with SU11274, a MET inhibitor, prevented HGF-induced AKT phosphorylation in MM cells and translated into reduced IDO protein levels and functional activity. Conclusions These data suggest that IDO1 expression may contribute to immune suppression in patients with MM and possibly other HGF-producing cancers.
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- 2012
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7. Upfront treatment with mTOR inhibitor everolimus in pediatric low-grade gliomas: A single-center experience.
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Cacchione, Antonella, Lodi, Mariachiara, Carai, Andrea, Miele, Evelina, Tartaglia, Marco, Megaro, Giacomina, Baldo, Giada Del, Alessi, Iside, Colafati, Giovanna Stefania, Carboni, Alessia, Boccuto, Luigi, Camassei, Francesca Diomedi, Catanzaro, Giuseppina, Po, Agnese, Ferretti, Elisabetta, Pedace, Lucia, Pizzi, Simone, Folgiero, Valentina, Pezzullo, Marco, and Corsetti, Tiziana
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EVEROLIMUS ,BRAIN tumors ,MTOR inhibitors ,MITOGEN-activated protein kinases ,GLIOMAS ,MAGNETIC resonance imaging ,CHILDREN'S hospitals - Abstract
Pediatric low-grade gliomas (pLGGs) are the most frequent brain tumor in children. Adjuvant treatment, consisting in chemotherapy and radiotherapy, is often necessary if a complete surgical resection cannot be obtained. Traditional treatment approaches result in a significant long-term morbidity, with a detrimental impact on quality of life. Dysregulation of the mitogen-activated protein kinase (MAPK) pathway is the molecular hallmark of pLGGs and hyperactivation of the downstream mammalian target of rapamycin (mTOR) pathway is frequently observed. We report clinical and radiological results of front-line treatment with everolimus in 10 consecutive patients diagnosed with m-TOR positive pLGGs at the Bambino Gesù Children's Hospital in Rome, Italy. Median duration of treatment was 19 months (range from 13-60). Brain magnetic resonance imaging showed stable disease in 7 patients, partial response in 1 and disease progression in 2. Therapy-related adverse events were always reversible after dose reduction or temporary treatment interruption. To the best of our knowledge, this is the first report of everolimus treatment for chemo- and radiotherapy-naïve children with pLGG. Our results provide preliminary support, despite low sample size, for the use of everolimus as target therapy in pLGG showing lack of progression with a manageable toxicity profile [ABSTRACT FROM AUTHOR]
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- 2021
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8. A new baby in the c-Myc-directed transcriptional machinery: Che-1/AATF.
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Folgiero, Valentina, Sorino, Cristina, Locatelli, Franco, and Fanciulli, Maurizio
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- 2018
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9. Metastatic Group 3 Medulloblastoma in a Patient With Tuberous Sclerosis Complex: Case Description and Molecular Characterization of the Tumor.
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Moavero, Romina, Folgiero, Valentina, Carai, Andrea, Miele, Evelina, Ferretti, Elisabetta, Po, Agnese, Diomedi Camassei, Francesca, Lepri, Francesca Romana, Vigevano, Federico, Curatolo, Paolo, Valeriani, Massimiliano, Colafati, Giovanna S., Locatelli, Franco, Tornesello, Assunta, and Mastronuzzi, Angela
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- 2016
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10. TIM-3/Gal-9 interaction induces IFNγ-dependent IDO1 expression in acute myeloid leukemia blast cells.
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Folgiero, Valentina, Cifaldi, Loredana, Li Pira, Giuseppina, Goffredo, Bianca Maria, Vinti, Luciana, and Locatelli, Franco
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ACUTE myeloid leukemia , *GENE expression , *BONE marrow , *GENETIC regulation , *CANCER chemotherapy - Abstract
NK cells expressing TIM-3 show a marked increase in IFNγ production in response to acute myeloid leukemia (AML) blast cells that endogenously express Gal-9. Herein, we demonstrate that NK cell-mediated production of IFNγ, induced by TIM-3/Gal-9 interaction and released in bone marrow microenvironment, is responsible for IDO1 expression in AML blasts. IDO1-expressing AML blasts consequently down-regulate NK cell degranulation activity, by sustaining leukemia immune escape. Furthermore, the blocking of TIM-3/Gal-9 interaction strongly down-regulates IFNγ-dependent IDO1 activity. Thus, the inhibition of TIM-3/Gal-9 immune check point, which affects NK cell-dependent IFNγ production and the consequent IDO1 activation, could usefully integrate current chemotherapeutic approaches. [ABSTRACT FROM AUTHOR]
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- 2015
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11. HIPK2 downregulates vimentin and inhibits breast cancer cell invasion.
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Nodale, Cristina, Sheffer, Michal, Jacob-Hirsch, Jasmine, Folgiero, Valentina, Falcioni, Rita, Aiello, Aurora, Garufi, Alessia, Rechavi, Gideon, Givol, David, and D'Orazi, Gabriella
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- 2012
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12. Induction of ErbB-3 Expression by α6β4 Integrin Contributes to Tamoxifen Resistance in ERβ1-Negative Breast Carcinomas.
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Folgiero, Valentina, Avetrani, Paolo, Bon, Giulia, Di Carlo, Selene E., Fabi, Alessandra, Nisticò, Cecilia, Vici, Patrizia, Melucci, Elisa, Buglioni, Simonetta, Perracchio, Letizia, Sperduti, Isabella, Rosanò1, Laura, Sacchi, Ada, Mottolese, Marcella, and Falcioni, Rita
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PROTEIN kinases , *PROTEIN-tyrosine kinases , *TAMOXIFEN , *BREAST cancer treatment , *ADJUVANT treatment of cancer , *RANDOMIZED controlled trials , *ESTROGEN antagonists , *CANCER cells , *PHOSPHORYLATION - Abstract
Background: Tamoxifen is still the most widely used drug in hormone therapy for the treatment of breast cancer. Its benefits in adjuvant treatment are well documented in controlled and randomized clinical studies, which have demonstrated an increase in disease-free intervals of patients with positive hormonal receptors. However, the mechanisms involved in endocrine resistance are not clear. Laboratory and clinical data now indicate that bi-directional molecular cross-talk between nuclear or membrane ER and growth factor receptor pathways may be involved in endocrine resistance. We recently found a functional interaction between α6β4 integrin and ErbB-3 receptor to maintain the PI3K/Akt survival pathway of mammary tumour cells. We sought to improve understanding of this process in order to provide the involvement of both receptors insight into mechanism of Tamoxifen resistance. Methods and Findings: Using human breast cancer cell lines displaying different levels of α6β4 and ErbB-3 receptors and a series of 232 breast cancer biopsies from patients submitted to adjuvant Tamoxifen monotherapy for five years, we evaluated the functional interaction between both receptors in relationship to Tamoxifen responsiveness. In mammary carcinoma cells, we evidenced that the α6β4 integrin strongly influence Akt phosphorylation through ErbB-3 protein regulation. Moreover, the ErbB-3 inactivation inhibits Akt phosphorylation, induces apoptosis and inhibits in vitro invasion favouring Tamoxifen responsiveness. The analysis of human tumors revealed a significant relationship between α6β4 and ErbB-3 in P-Akt-positive and ERβ1-negative breast cancers derived from patients with lower disease free survival. Conclusions: We provided evidence that a strong relationship occurs between α6β4 and ErbB-3 positivity in ERβ1-negative breast cancers. We also found that the association between ErbB-3 and P-Akt positivity mainly occurs in ERβ1-negative breast cancer derived from patients with lower DFS indicating that both receptors are clinically relevant in predicting the response to Tamoxifen. [ABSTRACT FROM AUTHOR]
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- 2008
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13. Enhancement of Neuroblastoma NK-Cell-Mediated Lysis through NF-kB p65 Subunit-Induced Expression of FAS and PVR, the Loss of Which Is Associated with Poor Patient Outcome.
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Brandetti, Elisa, Focaccetti, Chiara, Pezzolo, Annalisa, Ognibene, Marzia, Folgiero, Valentina, Cotugno, Nicola, Benvenuto, Monica, Palma, Paolo, Manzari, Vittorio, Rossi, Paolo, Fruci, Doriana, Bei, Roberto, and Cifaldi, Loredana
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THERAPEUTIC use of interferons ,EVALUATION of medical care ,TISSUE arrays ,NEUROBLASTOMA ,KILLER cells ,CELL receptors ,APOPTOSIS ,GENE expression ,CANCER patients ,CELL survival ,DNA-binding proteins ,TUMOR necrosis factors ,CELL lines ,TUMOR markers ,CELL death ,IMMUNOTHERAPY - Abstract
Simple Summary: Neuroblastoma (NB) cells adopt several molecular strategies to evade the Natural Killer (NK)-mediated response. Herein, we found that the overexpression of the NF-kB p65 subunit in NB cell lines upregulates the expression of both the death receptor FAS and the activating ligand PVR, thus rendering NB cells more susceptible to NK-cell-mediated apoptosis, recognition, and killing. These data could provide a clue for a novel NK-cell-based immunotherapy of NB. In addition, array CGH analysis performed in our cohort of NB patients showed that loss of both the FAS and PVR genes correlated with low survival, thus revealing a novel biomarker predicting the outcome of NB patients. High-risk neuroblastoma (NB) is a rare childhood cancer whose aggressiveness is due to a variety of chromosomal genetic aberrations, including those conferring immune evasion. Indeed, NB cells adopt several molecular strategies to evade recognition by the immune system, including the downregulation of ligands for NK-cell-activating receptors. To date, while molecular strategies aimed at enhancing the expression of ligands for NKG2D- and DNAM-1-activating receptors have been explored, no evidence has been reported on the immunomodulatory mechanisms acting on the expression of death receptors such as Fas in NB cells. Here, we demonstrated that transient overexpression of the NF-kB p65 subunit upregulates the surface expression of Fas and PVR, the ligand of DNAM-1, thus making NB cell lines significantly more susceptible to NK-cell-mediated apoptosis, recognition, and killing. In contrast, IFNγ and TNFα treatment, although it induced the upregulation of FAS in NB cells and consequently enhanced NK-cell-mediated apoptosis, triggered immune evasion processes, including the strong upregulation of MHC class I and IDO1, both of which are involved in mechanisms leading to the impairment of a proper NK-cell-mediated killing of NB. In addition, high-resolution array CGH analysis performed in our cohort of NB patients revealed that the loss of FAS and/or PVR genes correlated with low survival independently of the disease stage. Our data identify the status of the FAS and PVR genes as prognostic biomarkers of NB that may predict the efficacy of NK-cell-based immunotherapy of NB. Overall, restoration of surface expression of Fas and PVR, through transient upregulation of NF-kB, may be a clue to a novel NK-cell-based immunotherapy of NB. [ABSTRACT FROM AUTHOR]
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- 2021
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14. PI3K/Akt Pathway: The Indestructible Role of a Vintage Target as a Support to the Most Recent Immunotherapeutic Approaches.
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Caforio, Matteo, de Billy, Emmanuel, De Angelis, Biagio, Iacovelli, Stefano, Quintarelli, Concetta, Paganelli, Valeria, and Folgiero, Valentina
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PHOSPHOTRANSFERASES ,CELL physiology ,IMMUNE system ,ANTINEOPLASTIC agents ,CELLULAR signal transduction ,IMMUNOLOGICAL adjuvants ,TRANSFERASES ,TUMOR markers ,TUMORS ,IMMUNOTHERAPY - Abstract
Simple Summary: PI3K/Akt pathway has an impressive story as tumor marker. PI3K-dependent solid tumors have been studied for several years in order to inhibit the pathway at different levels along the signaling. Despite the highly satisfactory results obtained in vitro and in xenograft mouse tumor models, the use of PI3K/Akt inhibitors in clinical trials resulted in being not as efficient as expected. With the emerging role of the tumor microenvironment in the response to therapy and the awareness, increasing in recent years, of the necessity to army the immune system against the tumor, new opportunities have emerged for PI3K/Akt inhibitors. Here, we show that PI3K/Akt, in addition to its function as tumor marker, exerts a pivotal role as an immunomodulator. Recent studies demonstrate that PI3K/Akt pathway is crucial for the regulation of the immune system and that its inhibition in combination with immunomodulatory agents may provide a new therapeutic approach for cancer. Pathologic activation of PI3Ks and the subsequent deregulation of its downstream signaling pathway is among the most frequent events associated with cellular transformation, cancer, and metastasis. PI3Ks are also emerging as critical factors in regulating anti-tumor immunity by either promoting an immunosuppressive tumor microenvironment or by controlling the activity and the tumor infiltration of cells involved in the immune response. For these reasons, significant pharmaceutical efforts are dedicated to inhibiting the PI3K pathway, with the main goal to target the tumor and, at the same time, to enhance the anti-tumor immunity. Recent immunotherapeutic approaches involving the use of adoptive cell transfer of autologous genetically modified T cells or immune check-point inhibitors showed high efficacy. However, mechanisms of resistance to these kinds of therapy are emerging, due in part to the inhibition of effector T cell functions exerted by the immunosuppressive tumor microenvironment. Here, we first describe how inhibition of PI3K/Akt pathway contribute to enhance anti-tumor immunity and further discuss how inhibitors of the pathway are used in combination with different immunomodulatory and immunotherapeutic agents to improve anti-tumor efficacy. [ABSTRACT FROM AUTHOR]
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- 2021
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15. Proteomics Study of Peripheral Blood Mononuclear Cells in Down Syndrome Children.
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Lanzillotta, Chiara, Greco, Viviana, Valentini, Diletta, Villani, Alberto, Folgiero, Valentina, Caforio, Matteo, Locatelli, Franco, Pagnotta, Sara, Barone, Eugenio, Urbani, Andrea, Di Domenico, Fabio, and Perluigi, Marzia
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DOWN syndrome ,BLOOD cells ,PROTEOMICS ,CYTOSKELETON ,WESTERN immunoblotting ,DNA structure - Abstract
Down syndrome (DS) is the most common chromosomal disorder and the leading genetic cause of intellectual disability in humans, which results from the triplication of chromosome 21. To search for biomarkers for the early detection and exploration of the disease mechanisms, here, we investigated the protein expression signature of peripheral blood mononuclear cells (PBMCs) in DS children compared with healthy donors (HD) by using an in-depth label-free shotgun proteomics approach. Identified proteins are found associated with metabolic pathways, cellular trafficking, DNA structure, stress response, cytoskeleton network, and signaling pathways. The results showed that a well-defined number of dysregulated pathways retain a prominent role in mediating DS pathological features. Further, proteomics results are consistent with published study in DS and provide evidences that increased oxidative stress and the increased induction of stress related response, is a participant in DS pathology. In addition, the expression levels of some key proteins have been validated by Western blot analysis while protein carbonylation, as marker of protein oxidation, was investigated. The results of this study propose that PBMCs from DS children might be in an activated state where endoplasmic reticulum stress and increased production of radical species are one of the primary events contributing to multiple DS pathological features. [ABSTRACT FROM AUTHOR]
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- 2020
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16. Recent advances in searching c-Myc transcriptional cofactors during tumorigenesis.
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Caforio, Matteo, Sorino, Cristina, Iacovelli, Stefano, Fanciulli, Maurizio, Locatelli, Franco, and Folgiero, Valentina
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NEOPLASTIC cell transformation ,MYC oncogenes ,COFACTORS (Biochemistry) ,GENOMICS ,TRANSCRIPTION factors - Abstract
Background: The mechanism by which c-Myc exerts its oncogenic functions is not completely clear and different hypotheses are still under investigation. The knowledge of the capacity of c-Myc to bind exclusively E-box sequences determined the discrepancy between, on the one hand, genomic studies showing the binding of c-Myc to all active promoters and, on the other hand, the evidence that only 60% or less of the binding sites have E-box sequences. Main body: In this review, we provide support to the hypothesis that the cooperation of c-Myc with transcriptional cofactors mediates c-Myc-induced cellular functions. We produce evidence that recently identified cofactors are involved in c-Myc control of survival mechanisms of cancer cells. Conclusion: The identification of new c-Myc cofactors could favor the development of therapeutic strategies able to compensate the difficulty of targeting c-Myc. [ABSTRACT FROM AUTHOR]
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- 2018
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17. CAPE and its synthetic derivative VP961 restore BACH1/NRF2 axis in Down Syndrome.
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Pagnotta, Sara, Tramutola, Antonella, Barone, Eugenio, Di Domenico, Fabio, Pittalà, Valeria, Salerno, Loredana, Folgiero, Valentina, Caforio, Matteo, Locatelli, Franco, Petrini, Stefania, Butterfield, D. Allan, and Perluigi, Marzia
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DOWN syndrome , *LYMPHOBLASTOID cell lines , *REACTIVE nitrogen species , *HEME oxygenase , *SUPEROXIDE dismutase , *TRANSCRIPTION factors - Abstract
The cells possess several mechanisms to counteract the over-production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), including enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Moreover, an important sensor involved in the anti-oxidant response is KEAP1-NRF2-ARE signaling complex. Under oxidative stress (OS), the transcription factor NRF2 can dissociate from the KEAP1-complex in the cytosol and translocate into the nucleus to promote the transcriptional activation of anti-oxidant genes, such as heme oxygenase 1 and NADPH quinone oxidoreductase. Within this context, the activation of NRF2 response is further regulated by BACH1, a transcription repressor, that compete with the KEAP1-NRF2-ARE complex. In this work, we focused on the role of BACH1/NRF2 ratio in the regulation of the anti-oxidant response, proposing their antithetical relation as a valuable target for a therapeutic strategy to test drugs able to exert neuroprotective effects, notably in aging and neurodegenerative diseases. Among these, Down syndrome (DS) is a complex genetic disorder characterized by BACH1 gene triplication that likely results in the impairment of NRF2 causing increased OS. Our results revealed that BACH1 overexpression alters the BACH1/NRF2 ratio in the nucleus and disturbs the induction of antioxidant response genes ultimately resulting in the accumulation of oxidative damage both in Ts2Cje mice (a mouse model of DS) and human DS lymphoblastoid cell lines (LCLs). Based on this evidence, we tested Caffeic Acid Phenethyl Ester (CAPE) and the synthetic analogue VP961, which have been proven to modulate NRF2 activity. We showed that CAPE and VP961 administration to DS LCLs was able to promote NRF2 nuclear translocation, which resulted in the amelioration of antioxidant response. Overall, our study supports the hypothesis that BACH1 triplication in DS subjects is implicated in the alteration of redox homeostasis and therapeutic strategies to overcome this effect are under investigation in our laboratory. [Display omitted] • BACH-1/Nrf-2 has a fundamental role in the regulation of anti-oxidant response. • BACH-1 is among the genes encoded on HSA21 found to be overexpressed in Down Syndrome. • Targeting BACH-1/Nrf-2 axis is a valid therapeutic strategy in disease with increased Oxidative Stress. • Oxidative stress is an early event in Down Syndrome. • CAPE and VP961 are able to restore BACH-1/Nrf-2 axis in Down Syndrome. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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18. Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
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Lanzillotta, Chiara, Zuliani, Ilaria, Tramutola, Antonella, Barone, Eugenio, Blarzino, Carla, Folgiero, Valentina, Caforio, Matteo, Valentini, Diletta, Villani, Alberto, Locatelli, Franco, Butterfield, D. Allan, Head, Elizabeth, Perluigi, Marzia, Abisambra, Jose F., and Di Domenico, Fabio
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UNFOLDED protein response , *DOWN syndrome , *NEUROLOGICAL disorders , *NEURAL development , *PROTEIN synthesis - Abstract
• The PERK branch of the unfolded protein response is induced in DS and AD patients • Over-induced PERK increases eIF2α deregulation promoting translation repression • DS brain demonstrates the uncoupling between PERK and Nrf2-related antioxidant response • The inhibition of PERK in DS mice rescue proteins translation through the reduction of eIF2α • The inhibition of PERK rebalances Nrf2/Bach1 nuclear levels and decrease brain oxidative damage A major challenge in neurobiology is the identification of the mechanisms by which protein misfolding leads to cellular toxicity. Many neurodegenerative disorders, in which aberrant protein conformers aggregate into pathological inclusions, present the chronic activation of the PERK branch of the unfolded protein response. The adaptive effects of the PERK pathway include reduction of translation by transient inhibition of eIF2α and antioxidant protein production via induction of Nrf2 transcription factor. In contrast, PERK prolonged activation leads to sustained reduction in protein synthesis and induction of cell death pathways. To further investigate the role of the PERK pathway in neurodegenerative disorders, we focused on Down syndrome (DS), in which aging confers a high risk of Alzheimer disease (AD). By investigating human DS frontal cortices, we found early and sustained PERK activation associated with the induction of eIF2α and ATF4 downstream signals. We also observed that the Nrf2 response is uncoupled from PERK and its antioxidant effects are repressed in a mechanism implicating the transcription repressor Bach1. The pharmacological inhibition of PERK in DS mice reduced eIF2α-related translational repression and promoted Nrf2 nuclear translocation, favoring the rescue of Nrf2/Bach1 imbalance. The further analysis of peripheral cells from living DS individuals provided strong support of the pathological link between PERK and trisomy 21. Our results suggest that failure to regulate the PERK pathway is a peculiar characteristic of DS pathology and it may represent an essential step to promote cellular dysfunction, which actively contributes in the brain to the early development of AD. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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