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Electrophoretic investigations of the serum protein patterns of natterjacks ( Bufo calamita Laur.) from the Iberian Peninsula and from Western and Central Europe, and of green toads ( Bufo viridis Laur.) from Central Europa, Asia Minor and North Africa show that the two species differ disctinctly in the position of their albumin fractions. The identification of problematic specimens can result from the production of mixed serum with specimens of both species. As the albumin fraction of hybrids is doubled according to this method, it is also suitable for demonstrating the existence of such hybrids in natural populations. [ABSTRACT FROM AUTHOR]