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This paper presents the results of the blind test competition carried out within the scope of the European project SERA.ta "Seismic Response of Masonry Cross Vaults: Shaking table tests and numerical validations". The purpose of the competition was to predict the results of a set of tests carried out on an unstrengthened full-scale cross vault with mortar joints and solid bricks (then strengthened with textile reinforced mortar) subjected to a horizontal dynamic excitation. The paper offers an overview of the modelling approaches utilised, along with their corresponding predictions and post dictions. The findings are assessed based on both the damage mechanisms and predicted values for displacements and accelerations in both directions. The results are then compared with the experimental findings. Modelling approaches utilizing the Finite Element Method (FEM) yielded the most accurate predictions regarding displacements and crack patterns. Conversely, a submission employing a Discrete Element model provided the most accurate prediction of damage mechanisms. Nonetheless, the significant discrepancies in predicted displacements and accelerations underscore the necessity for continued efforts to establish consensus on appropriate modelling assumptions for masonry vaults. [ABSTRACT FROM AUTHOR]

Gabriella Di Felice,1 Paolo Colombo2 1National Center for Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, 2Institute of Biomedicine and Molecular Immunology, National Research Council, Palermo, Italy Abstract: Allergen-specific immunotherapy was introduced in clinical settings more than 100 years ago. It remains the only curative approach to treating allergic disorders that ameliorates symptoms, reduces medication costs, and blocks the onset of new sensitizations. Despite this clinical evidence and knowledge of some immunological mechanisms, there remain some open questions regarding the safety and efficacy of this treatment. This suggests the need for novel therapeutic approaches that attempt to reduce the dose and frequency of treatment administration, improving patient compliance, and reducing costs. In this context, the use of novel adjuvants has been proposed and, in recent years, biomedical applications using nanoparticles have been exploited in the attempt to find formulations with improved stability, bioavailability, favorable biodistribution profiles, and the capability of targeting specific cell populations. In this article, we review some of the most relevant regulatory aspects and challenges concerning nanoparticle-based formulations with immunomodulatory potential, their related immunosafety issues, and the nature of the nanoparticles most widely employed in the allergy field. Furthermore, we report in vitro and in vivo data published using allergen/nanoparticle systems, discuss their impact on the immune system in terms of immunomodulatory activity and the reduction of side effects, and show that this strategy is a novel and promising tool for the development of allergy vaccines. Keywords: allergy, nanocarriers, immunotoxicity, immune modulation, immunotherapy, allergens

Se efectuó una encuesta transversal en 350 establecimientos de ciclo completo y cría (CC) e invernada (IN) bovina, ubicados el este de la provincia de La Pampa con la finalidad de registrar los casos de gastroenteritis verminosa (GEV) y las prácticas de control y manejo utilizadas por los ganaderos. La muestra fueron tomadas al azar a partir de 3 estratos de acuerdo al número de existencias bovinas: productores con más de 900 (G), de entre 900-500 (M) y de 500 a 300 (P) bovinos. El 33 % de los productores encuestados declaró haber tenido casos clínicos de GEV con una morbilidad y mortalidad del 11.2% y 0.42% respectivamente. Se registraron más casos de GEV (P

A cross-sectional survey including 350 stocking farms and bovine establishments that operate the full cycle (FC) as well as fattening operations (IN) was carried out in the East of the Province of La Pampa to record cases of verminous gastroenteritis (VGE) as well as control and management practices applied by the farmers.Farms were stratified by herd size into three categories: more than 900 (G), 900-500 (M) and 500-300 (P) bovines. Samples from each category were collected at random. 33% of the farmers surveyed indicated that their animals had been affected by clinical cases of VGE with morbidity and mortality rates between 11.2% and 0.42%, respectively. More cases of VEG (P

The analysis of deformation and damage mechanisms induced by shallow tunnelling on masonry structures is carried out using an integrated, geotechnical and structural, numerical approach based on two-dimensional finite-element analyses. The masonry construction, schematised as a block structure with periodic texture, is regarded at a macroscopic scale as a homogenised anisotropic medium. The overall mechanical properties display anisotropy and singularities in the yield surface, arising from the discrete nature of the block structure and the geometrical arrangement of the blocks. The soil is modelled by means of a linear elastic-perfectly plastic model. The numerical analyses are performed assuming plane strain and plane stress conditions for the soil and the masonry structure, respectively. A displacement-controlled technique is adopted to simulate the tunnel construction, which produces settlement troughs in agreement with the empirical Gaussian predictions at different volume losses under free-field conditions. In order to test the numerical approach, a preliminary set of parametric analyses is carried out considering a simple masonry wall, characterised by different geometrical and mechanical properties, founded on a clayey deposit. Then, the case study of the Felice aqueduct in Rome (Italy), undercrossed by two tunnels of a new metro line, is considered. Significant differences are observed between the uncoupled analysis, where displacements predicted under free-field conditions are simply applied at the foundation level of the structure, and the interaction-based one, the latter being characterised by a reduced amount of tensile plastic strain. Numerical results in terms of vertical displacements at the ground level and on the structure are found to be in good agreement with monitoring data, thus validating the numerical model for this class of soil-structure interaction problems. [ABSTRACT FROM AUTHOR]

Summary Background Parietaria pollen is one of the major cause of pollinosis in the southern Europe. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease restoring a normal immunity against allergens. Methods We designed a recombinant molecule ( Pj EDloop1) comprised of genetic-engineered variants of the major allergens of the Parietaria pollen ( Par j 2/ Par j 1). Purity and chemical-physical properties of the derivative were analysed by RP- HPLC chromatography and Photon Correlation Spectroscopy. Immunological activity was evaluated by means of Western blotting, ELISA inhibition and PBMC proliferation assay in 10 Parietaria allergic patients. Basophil activation was studied in six subjects. The immunogenicity of the hybrid was studied looking at the immune responses induced in a mouse model of sensitization. Results The Pj EDloop1 hybrid was produced as a purified recombinant protein with high stability in solution. Western blot, ELISA inhibition and basophil activation test showed that the Pj EDloop1 displays a remarkable reduced IgE binding and anaphylactic activity. CD3 reactivity was conserved in all patients. Mice immunization with the r Pj EDloop1 induced antibodies and T cell responses comparable to that obtained by the wild type allergens. Such antibodies shared the specificities to r Par j 1 and r Par j 2 with human IgE antibodies. Conclusion Our results demonstrated that a mutant hybrid expressing genetically engineered forms of the major P. judaica allergens displayed reduced allergenicity and retained T cell reactivity for the induction of protective antibodies in vaccination approaches for the treatment of Parietaria pollinosis. [ABSTRACT FROM AUTHOR]

The presence of specific IgE antibodies to invertebrates is common among patients with rhinitis and asthma. Tropomyosin has been described as an invertebrate cross-reactive allergen. We have recently characterized an allergenic extract from silverfish (Lepisma saccharina). Since this insect could be a new source of tropomyosin in the indoor environment, we have thought important to clone and characterize the tropomyosin from it.Recombinant tropomyosin was cloned and characterized by means of immunoblotting withtropomyosin-specific monoclonal antibodies, rabbit polyclonal antibodies and IgE from allergic patients. Its allergenic activity was investigated in histamine release assays. Immunoblotting and ELISA inhibition were carried out to identify the natural tropomyosin in the silverfish extract and to study the cross-reactivity among other arthropod tropomyosins.Tropomyosin-specific antibodies recognized in immunoblotting the natural tropomyosin in the insoluble fraction of silverfish extract. The silverfish tropomyosin (Lep s 1) was cloned and fully expressed. It shared high homology with other arthropod tropomyosins. rLep s 1 was recognized by tropomyosin-specific monoclonal and polyclonal antibodies and by IgE of allergic patients. It was able to inhibit the IgE binding to the insoluble fraction of silverfish extract, and to induce histamine release by an arthropod-allergic serum. Inhibition experiments revealed IgE cross-reactivity between rLep s 1 and other arthropod tropomyosins.rLep s 1 is the first allergen cloned and characterized from silverfish extract. It enabled us to identify the natural counterpart in the insoluble fraction of silverfish extract, suggesting that the tropomyosin is not readily extractable with a classic aqueous extraction procedure. rLep s 1 displayed biological activity, suggesting that it could be regarded as a useful tool to study the role of silverfish tropomyosin in the sensitization to invertebrate allergic sources. [ABSTRACT FROM AUTHOR]

Lymphocytosis is a marker of subcutaneous interleukin (IL)-2 therapy efficacy, whereas baseline elevated inflammatory indices were noticed in IL-2-resistant disease. The aim of this study was to analyse the relationship between pretreatment circulating values of IL-6, neopterin, sIL-2R, ESR and the changes in lymphocyte number in response to IL-2 administration. Twenty metastatic renal cell cancer patients were treated with subcutaneous IL-2 immunotherapy (6 000 000 IU day[SUP-1] for 6 days per week for 4 weeks); tumour response consisted of partial response (PR) in four patients, stable disease (SD) in eight patients and progressive disease (PD) in eight patients. Abnormally high pretreatment values of each marker were found as follows: IL-6 in seven patients, neopterin in nine patients, sIL-2R in 13 patients. In response to IL-2 immunotherapy, a significantly higher mean increase in lymphocyte number and a higher percentage of patients with tumour response or stable disease were observed when pretreatment values of IL-6, neopterin and sIL-2R were within the normal range, in comparison to patients with high values for these markers. The pretreatment excess of these serum inflammatory markers seems to negatively influence both the host and tumour response to IL-2 administration, by preventing the IL-2-induced lymphocytosis and resulting in tumour progression. Further studies are requested to verify if overall survival and quality of life may depend on pretreatment host immune status and/or lymphocyte response after IL-2 administration. [ABSTRACT FROM AUTHOR]

We have carried out a retrospective review of 61 patients with Mallory-Weiss syndrome, 9 of whom underwent hemostatic injection treatment. At emergency endoscopy, 3 patients (5%) were actively bleeding and 6 (10%) had stigmata of recent hemorrhage. Fifty-two patients had a lesion of the cardia which was the suspected source of bleeding in 33 cases (54%). In the remaining 19 cases (31%) this site was not considered responsible because other sites of bleeding were present in the upper gastrointestinal tract. The 9 patients with active bleeding or stigmata of recent hemorrhage underwent injection treatment and the other 52 patients were treated with H2 antagonists. The low rebleed rate and the zero mortality recorded in this series indicate that endoscopy is the optimum method for diagnosis and treatment of patients with Mallory-Weiss syndrome. [ABSTRACT FROM AUTHOR]

A case of primary carcinoma of the esophagus is presented that consisted of poorly differentiated small-cell carcinoma. The patient underwent endoscopic treatment, first with an Nd: YAG laser and, finally, with bougienage and the insertion of a prosthesis in order to relieve dysphagia and improve the nutritional status and quality of life. Even though it is generally uncommon for laser therapy to be chosen as a primary mode of treatment, the results obtained should permit to overcome this lack of confidence in the choice of laser treatment for small-cell carcinoma of the esophagus. [ABSTRACT FROM AUTHOR]