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The last ten years have seen the discovery of free radicals and their damaging impacts. Increasing exogenous antioxidant intake could reduce the damage caused by oxidative stress. Several plants have been shown to have antioxidant activity, and one such plant is BasellaI. It is high in phytochemicals which can act as antioxidants, and its consumption may help fight free radicals generated by the body. In particular, this plant is essential for stimulating normal wound healing response. To the best of our knowledge, no bibliometric analysis of published data on Basella as an antioxidant has been done. The goal of this study is to conduct a bibliometric analysis of the research on Basella’s antioxidant properties in the Scopus database using the VOSviewer and RStudio tools. There were 56 articles on Basella as an antioxidant according to the bibliometric analysis. The countries with the highest research output was India (27 documents), and the most productive institution was Chiang Mai University (15 documents). The most productive source was the International Journal of Pharmacy and Pharmaceutical Sciences. P. Giridhar had significant significant impact on papers on Basella as an antioxidant (H-index of 5). The most common keywords were “antioxidant” (859 occurrences with 1,340 total link strength) and “Basella alba” (606 occurrences with 1,048 total link strength). Findings from this data suggest the novelties of Basella as an antioxidant.

Abstract Background BamHI-A rightward frame-1 (BARF1) is a carcinoma-specific Epstein-Barr virus (EBV) encoded oncogene. Here we describe the BARF1 sequence diversity in nasopharyngeal carcinoma (NPC), other EBV-related diseases and Indonesian healthy EBV carriers in relation to EBV genotype, viral load and serology markers. Nasopharyngeal brushings from 56 NPC cases, blood or tissue from 15 other EBV-related disorders, spontaneous B cell lines (LCL) from 5 Indonesian healthy individuals and several prototype EBV isolates were analysed by PCR-direct sequencing. Results Most NPC isolates revealed specific BARF1 nucleotide changes compared to prototype B95-8 virus. At the protein level these mutations resulted in 3 main substitutions (V29A, W72G, H130R), which are not considered to cause gross tertiary structure alterations in the hexameric BARF1 protein. At least one amino acid conversion was detected in 80.3% of NPC samples compared to 33.3% of non-NPC samples (p < 0.001) and 40.0% of healthy LCLs (p = 0.074). NPC isolates also showed more frequent codon mutation than non-NPC samples. EBV strain typing revealed most isolates as EBV type 1. The viral load of either NPC or non-NPC samples was high, but only in non- NPC group it related to a particular BARF1 variant. Serology on NPC sera using IgA/EBNA-1 ELISA, IgA/VCA-p18 ELISA and immunoblot score showed no relation with BARF1 sequence diversity (p = 0.802, 0.382 and 0.058, respectively). NPC patients had variable antibody reactivity against purified hexameric NPC-derived BARF1 irrespective of the endogenous BARF1 sequence. Conclusion The sequence variation of BARF1 observed in Indonesian NPC patients and controls may reflect a natural selection of EBV strains unlikely to be predisposing to carcinogenesis. The conserved nature of BARF1 may reflect an important role in EBV (epithelial) persistence.