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Catalase (CAT), glutathione peroxidase (GPx), and peroxiredoxin 2 (Prx2) can counteract the deleterious effects of oxidative stress (OS). Their binding to the red blood cell (RBC) membrane has been reported in non-immune hemolytic anemias (NIHAs). Our aim was to evaluate the relationships between CAT, GPx, and Prx2, focusing on their role at the RBC membrane, in hereditary spherocytosis (HS), sickle cell disease (SCD), β-thalassemia (β-thal), and healthy individuals. The studies were performed in plasma and in the RBC cytosol and membrane, evaluating OS biomarkers and the enzymatic activities and/or the amounts of CAT, GPx, and Prx2. The binding of the enzymes to the membrane appears to be the primary protective mechanism against oxidative membrane injuries in healthy RBCs. In HS (unsplenectomized) and β-thal, translocation from the cytosol to the membrane of CAT and Prx2, respectively, was observed, probably to counteract lipid peroxidation. RBCs from splenectomized HS patients showed the highest membrane-bound hemoglobin, CAT, and GPx amounts in the membrane. SCD patients presented the lowest amount of enzyme linkage, possibly due to structural changes induced by sickle hemoglobin. The OS-induced changes and antioxidant response were different between the studied NIHAs and may contribute to the different clinical patterns in these patients.

Acute porphyrias are a group of rare genetic metabolic disorders, caused by a defect in one of the enzymes involved in the heme biosynthesis, which results in an abnormally high accumulation of toxic intermediates. Acute porphyrias are characterized by potentially life-threatening attacks and, for some patients, by chronic manifestations that negatively impact daily functioning and quality of life. Clinical manifestations include a nonspecific set of gastrointestinal, neuropsychiatric, and/or cutaneous symptoms. Effective diagnostic methods are widely available, but due to their clinical heterogeneity and non-specificity, many years often elapse from symptom onset to diagnosis of acute porphyrias, delaying the treatment and increasing morbidity. Therefore, increased awareness of acute porphyrias among healthcare professionals is paramount to reducing disease burden. Treatment of acute porphyrias is centered on eliminating the potential precipitants, symptomatic treatment, and suppressing the hepatic heme pathway, through the administration of hemin or givosiran. Moreover, properly monitoring patients with acute porphyrias and their relatives is fundamental to preventing acute attacks, hospitalization, and long-term complications. Considering this, a multidisciplinary panel elaborated a consensus paper, aiming to provide guidance for an efficient and timely diagnosis of acute porphyrias, and evidence-based recommendations for treating and monitoring patients and their families in Portugal. To this end, all authors exhaustively reviewed and discussed the current scientific evidence on acute porphyrias available in the literature, between November 2022 and May 2023.

The antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and peroxiredoxin 2 (Prx2) are particularly important in erythroid cells. Reticulocytes and other erythroid precursors may adapt their biosynthetic mechanisms to cell defects or to changes in the bone marrow environment. Our aim was to perform a comparative study of the mRNA levels of CAT, GPX1, PRDX2 and SOD1 in reticulocytes from healthy individuals and from patients with hereditary spherocytosis (HS), sickle cell disease (SCD) and β-thalassemia (β-thal), and to study the association between their transcript levels and the reticulocyte maturity indices. In controls, the enzyme mRNA levels were significantly correlated with reticulocyte maturity indices for all genes except for SOD1. HS, SCD and β-thal patients showed younger reticulocytes, with higher transcript levels of all enzymes, although with different patterns. β-thal and HS showed similar reticulocyte maturity, with different enzyme mRNA levels; SCD and HS, with different reticulocyte maturity, presented similar enzyme mRNA levels. Our data suggest that the transcript profile for these antioxidant enzymes is not entirely related to reticulocyte maturity; it appears to also reflect adaptive mechanisms to abnormal erythropoiesis and/or to altered erythropoietic environments, leading to reticulocytes with distinct antioxidant potential according to each anemia.

Immunity to Ascaris lumbricoides influences the pathogenesis of allergic diseases. Antibody responses to its proteins have been found to be associated with asthma presentation; however, helminth products that induce immunosuppression have been reported, which also raise specific antibodies. We aimed to evaluate antibody responses (IgE, IgG4 and IgG) to two A. lumbricoides molecules, Asc l 5 and Al-CPI (an anti-inflammatory Cysteine Protease Inhibitor), in an endemic population, exploring their relationships with the infection and asthma. The two molecules were produced as recombinant proteins in E. coli expression systems. Specific antibodies were detected by ELISA. Lower human IgE, but higher IgG4 and IgG antibody levels were observed for Al-CPI than for rAsc l 5. The IgE/IgG4 isotype ratio was significantly higher for Asc l 5 than for Al-CPI. In humans Al-CPI did not induce basophil activation as has been previously described for Asc l 5. In mice, Al-CPI induced fewer IgE responses, but more IgG2a antibody titers than rAsc l 5. Our results suggest that these molecules elicit different patterns of immune response to A. lumbricoides.

Although vertical transmission of CHIKV has been reported, little is known about the role of placenta in the transmission of this virus and the effects of infection on the maternal-fetal interface. In this work we investigated five placentas from pregnant women who became infected during the gestational period. Four formalin-fixed paraffin-embedded samples of placenta (cases 1–4) were positive for CHIKV by RT-PCR. One (case 5) had no positive test of placenta, but had positive RT-PCR for CHIKV in the serum of the mother and the baby, confirming vertical transmission. The placentas were analyzed regarding histopathological and immunological aspects. The main histopathological changes were: deciduitis, villous edema, deposits, villous necrosis, dystrophic calcification, thrombosis and stem vessel obliteration. In infected placentas we noted increase of cells (CD8+ and CD163+) and pro- (IFN-γ and TNF-α) and anti-inflammatory (TGF-β and IL-10) cytokines compared to control placentas. Moreover, CHIKV antigen was detected in decidual cell, trophoblastic cells, stroma villi, Hofbauer cells, and endothelial cells. In conclusion, CHIKV infection seems to disrupt placental homeostasis leading to histopathological alterations in addition to increase in cellularity and cytokines overproduction, evidencing an altered and harmful environment to the pregnant woman and fetus.

INTRODUÇÃO: Durante a pandemia COVID-19, os quadros de burnout parecem ter-se agravado nos médicos. Como tal, pretendeu-se determinar a prevalência de burnout, reflexo da pandemia COVID-19, nos médicos de família de um Agrupamento de Centros de Saúde. MATERIAL E MÉTODOS: Estudo analítico descritivo transversal, com questionário anónimo de autorresposta, durante abril e maio de 2021. Aplicação do Maslach Burnout Inventory-Health Services Survey. RESULTADOS: Obtiveram-se 60 respostas válidas. Amostra predominantemente feminina (82%), entre os 36 e 45 anos (53%). Trabalhavam numa Unidade de Saúde Familiar 78%, e 22% numa Unidade de Cuidados de Saúde Personalizados. Afirmaram ter pensado mudar de profissão, instituição ou serviço no último mês, 52%. Apresentaram elevada exaustão emocional 70%, 40% elevada despersonalização e 45% baixa realização pessoal. Apresentaram burnout 32%. A exaustão emocional foi maior para carga horária semanal >40 horas (p=0,011). Baixa realização pessoal (p=0,004) e burnout (p=0,034) revelaram-se mais prevalentes nas faixas etárias mais baixas. Não se observaram relações estatisticamente significativas entre burnout e sexo ou local de trabalho. DISCUSSÃO: A prevalência estimada de burnout nos médicos de família é duas vezes superior à de 2016 na zona norte e oito vezes superior à nacional de 2012. Dada a relação temporal, é provável existir uma relação deste aumento de prevalência com a pandemia COVID-19. A maior prevalência de burnout nos mais jovens poderá evidenciar estratégias de coping com o stress ocupacional menos efetivas. CONCLUSÃO: A prevenção do burnout nos médicos de família deve ser uma prioridade, dada a sua elevada prevalência e impacto na saúde mental, bem como forma de prevenção quinquenária em saúde.

O município de Cruzeiro do Sul, o segundo mais populoso do estado do Acre, é abastecido majoritariamente por águas subterrâneas. O aquífero mais utilizado na região é o Içá-Solimões, do qual pouco se conhece apesar de ser muito explotado há décadas. No presente trabalho objetiva-se, portanto, estudar e caracterizar o Sistema Aquífero Içá-Solimões na região de Cruzeiro do Sul, visando minorar essa lacuna de conhecimento. O estudo baseou-se no levantamento de dados de poços tubulares cadastrados no portal SIAGAS da CPRM e no inventário de poços fornecido pelo departamento de abastecimento do Acre, bem como na obtenção de dados in situ durante campanha de campo realizada na região, incluindo o nivelamento de dados piezométricos, tomada de amostras e análises químicas e isotópicas realizadas em laboratório sobre essas amostras e sua posterior interpretação. Buscou-se compreender melhor as características de funcionamento hidrogeológico desse sistema. A partir dos resultados alcançados foi possível identificar ao menos três camadas com características hidrogeológicas individualizáveis no sistema, abrigadas em arenitos que se intercalam com argilitos. O mapa de nível piezométrico do sistema aquífero mostrou uma tendência local de fluxo das áreas mais elevadas da cidade, em relevo de tabuleiros, em direção aos rios. A interpretação das análises químicas permitiu a identificação de águas predominantemente bicarbonatadas-sódicas e, secundariamente, bicarbonatadas-cálcicas, oriundas da dissolução de minerais silicáticos, segundo a interpretação realizada. As camadas mais profundas apresentam química distinta das camadas mais rasas, apontando para processos diferenciados de interação água-rocha, tanto espacial como temporalmente. Os resultados isotópicos mostraram a ocorrência de águas antigas, nas camadas aquíferas mais profundas, com cerca de 18 ka. Desse modo, as áreas de recarga em potencial do aquífero profundo situa-se a oeste, muito provavelmente em território peruano

ABSTRACT Objective: To analyze information on resource allocation in the context of the COVID-19 pandemic, published in indexed scientific journals, from December 2019 to March 2020. Methods: This is an integrative literature review, which took place in March 2020. All databases were investigated and studies were found only in MEDLINE. After applying the established criteria, six articles were selected. Results: It was evident that the allocation of resources is carried out as the demands emerge. The fragility in presenting scientific-methodological evidence that can guide decision makers for assertive allocation of available resources is highlighted. The results showed that studies on this subject are incipient and need to be expanded. Final considerations: The need for health organizations and area authorities to be better prepared for the proper use of available resources, with allocation based on scientific evidence and maximization of resources is indicated.

Introduction: Ketogenic diet is a low carbohydrate diet, which can be used as a treatment for refractory childhood epilepsy. The first aim of this study was to evaluate its efficacy, in patients receiving ketogenic diet for at least three months, on epilepsy control, behaviour and awareness. The secondary aims were to evaluate the variation in the number of antiepileptic drugs, reasons for discontinuing the diet and adverse effects. Material and Methods: Retrospective analysis of clinical records of patients who underwent ketogenic diet for refractory epilepsy, from October 2007 to January 2018, in a tertiary pediatric hospital. Results: In the twenty-nine eligible patients, the mean age of initiation was 7.9 years-old (+/– 5.6). Of those, 18 had a ≥ 50% reduction of seizure activity, 19 a marked behaviour improvement and 18 improved awareness. The median number of antiepileptic drugs remained equal for the 15 patients who completed 18 months of treatment (three drugs). The main reason for discontinuing ketogenic diet was a familiar decision. The main adverse effects were hypercholesterolemia (n = 23) and hypertriglyceridemia (n = 21). Discussion: Results were comparable to those of other cohorts, namely age of initiation, proportion of patients completing ketogenic diet, most frequent reasons for stopping and significant improvement of alertness and behavior. Conclusion: Beyond seizure control, patients experienced a marked improvement in behavior and awareness. It is necessary to develop strategies to increase the adherence of families to the diet.

A gestão de custos é a ação de gerir recursos e aprimorar processos utilizando informações sobre custos para subsidiar a alocação de recursos e o processo decisório. O objetivo deste trabalho é identificar o uso das informações de custos em serviços públicos de urgência e emergência segundo a visão de gestores de custos. Trata-se de uma pesquisa de campo com abordagem qualitativa, sendo o estudo de caso adotado como método. Participaram da pesquisa 08 gestores de custos. Foi aplicado um formulário para caracterizar o perfil dos gestores e foram realizadas entrevistas com roteiro semiestruturado. Todos os gestores de custos entrevistados afirmaram que adotam duas metodologias de custeio: Custeio por Absorção e ABC. Os dados de produção dos centros de custos são processados, dando origem a relatórios que carregam as informações de custos. Um desses relatórios é a Conta Paciente, que visa informar ao usuário sobre os custos hospitalares envolvidos na assistência prestada. As análises realizadas a partir das informações dos relatórios são discutidas mensalmente nos chamados Observatórios de Custos. No geral, os entrevistados afirmaram que as informações de custos são utilizadas para a tomada de decisão nos hospitais.

Abstract Objective: to analyze the care and management demands of Long-Term Care Facilities for the Elderly (LTCFs) in Brazil. Method: an integrative review of literature was carried out, organized into six stages: a) elaboration of a guiding question; b) online search of LILACS, SciELO, PubMed, the CAPES Portal and the Brazilian Society of Geriatrics and Gerontology databases; c) article selection, following the exclusion and inclusion criteria, with the sample composed of 17 articles; d) commented analysis of the selected articles; e) deliberation on the results obtained, formulated from the synthesis and interpretation of the selected studies; f) presentation of the results of the review. Results: the care demands identified are related to the process of caring and assume a working team with geriatric and gerontological knowledge, while the management demands include the means and resources needed so the care can be provided effectively. However, the LTCFs were found to rely on professionals who are unprepared to provide care or to assume an organizational management role, meaning care for the elderly is restricted to the essentials for their basic needs. Conclusion: the care demands were easily identified in the analyzed publications, however, there is a lack of research that evaluates management demands in a broader and more in-depth manner. It is suggested that studies aiming to broaden theoretical knowledge of the care and management demands of LTCFs are carried out, to stimulate effective and positive actions in the practices of these institutions, seeking to offer top quality care to elderly persons that live in these facilities, that responds to the real needs of their current stage of life.

The objective of this study was to compare the professional identity perceptions among undergraduate students enrolled in predominantly female and male courses. The research method is cross-sectional and the sample consisted of 502 undergraduate students in the fields of health and engineering. A questionnaire with the Scale of Professional Self and Hetero-Perception (EAHP) was used to collect the data and descriptive statistics, exploratory factor analysis, and structural equations modeling were used as analysis techniques. According to the students from the two areas, the dimensions that best describe their professions are dynamism; technicity; effort; and ethics, while in the health field, the dimension that received the lowest average score was recognition, indicating that the professionals working in this field resent the lack of respect, admiration, and prestige in society, despite perceiving themselves as honest, honored, productive, and hardworking. Also, the average hetero-perception scores were lower for the health students and the difference between self and hetero-perception was less significant among the engineering students. The results confirm that the professional identities include gender-related attributes, leading to the conclusion that health professions remain vulnerable to gender domination relations.

Resumo: Introdução: A miocardiopatia hipertrófica (MCH) é uma patologia com transmissão essencialmente autossómica dominante, expressão clínica variável e penetrância incompleta. O rastreio familiar tem por objetivo identificar a ocorrência ou o risco de desenvolvimento da doença nos parentes em primeiro grau do caso índex. As normas de orientação da ESC e da ACCF/AHA recomendam a avaliação dos familiares em idade pediátrica a partir dos 10‐12 anos. Objetivos: Avaliaram‐se os resultados de um programa de rastreio pediátrico de MCH familiar e o valor preditivo do estudo genético nexte contexto. Foi ainda aferida a penetrância fenotípica ao longo do tempo de seguimento destas crianças. Métodos e resultados: Foram incluídas 20 crianças pertencentes a dez famílias (2004‐2013). Três das crianças constituíram‐se como o caso índex, sendo as restantes parentes em primeiro grau de um doente com MCH (80% sexo masculino; idade mediana = 10 anos). Catorze crianças eram portadoras de mutação de um gene sarcomérico (70%; idade mediana = 8 anos). Sete (50%) dos 14 portadores de mutação apresentavam fenótipo positivo na primeira avaliação.Foram definidos como «familiares em risco» aqueles com teste genético positivo, mas com fenótipo normal à apresentação. Após 3,5 ± 0,8 anos de seguimento, duas das crianças fenótipo negativo portadoras de mutação (gene MYBPC3) desenvolveram MCH, aos dez e 15 anos de idade (28% de taxa de penetrância). Conclusões: A penetrância de MCH em crianças com fenótipo normal à apresentação foi de 28% após 3,5 anos de seguimento. Tal sublinha a importância da avaliação longitudinal dos portadores de mutação de genes sarcoméricos, independentemente da presença de fenótipo patológico. Abstract: Introduction: Hypertrophic cardiomyopathy (HCM) is most often of autosomal dominant inheritance with incomplete penetrance and variable expression. The main purpose of family screening is to identify relatives with unrecognized HCM and to monitor those at risk for disease, in order to minimize complications and to assess risk of sudden cardiac death. The ESC and ACCF/AHA guidelines on the diagnosis and management of HCM recommend the screening of child relatives from the age of 10‐12 years. Objectives: We studied the outcome of clinical screening and genetic testing of child probands and relatives (

Essa pesquisa teve como objetivo geral descrever o processo de construção de uma escala para análise das competências profissionais de gestores da área da saúde. Na etapa exploratória foi realizado um painel de especialistas. Na segunda etapa, cento e dois gestores responderam ao questionário. Para depuração da base, foi realizada a análise descritiva e multivariada dos dados. Adicionalmente, foi realizada a análise da dimensionalidade, confiabilidade e validade da escala, resultando em uma escala com 20 competências técnicas, distribuídas em cinco eixos temáticos: economia e demografia; política, planejamento e avaliação em saúde; gestão do trabalho e educação em saúde; administração e contabilidade; e metodologias estruturantes. As competências comportamentais mapeadas totalizaram 16 e foram distribuídas nos eixos: idealista/perfeccionista, artesão/negociador, prestativo/guardião, motivador/racional.

Introdução: O burnout é um estado de exaustão física, emocional e mental, causado pelo envolvimento duradouro em situações de elevada exigência emocional, no local de trabalho. Os médicos têm uma das profissões consideradas mais stressantes, sendo que os Médicos de Medicina Geral e Familiar (MGF) são dos que estão em maior risco de burnout. As estratégias de coping adotadas são cruciais para a transição de níveis de stress aumentado para burnout. Objetivos: Avaliar a prevalência e a gravidade do índice de burnout nos médicos especialistas e internos de MGF, em funções nos Cuidados de Saúde Primários (CSP) pertencentes à Uni- dade de Saúde Local de Matosinhos (ULSM); avaliar a relação entre o índice de burnout e variáveis sócio-demográficas e profissionais nesta amostra; avaliar a relação entre o índice de burnout e estratégias de coping dos profissionais na amostra. Métodos: Realizou-se um estudo observacional, descritivo e transversal, com componente analítica. Foi aplicado um questionário sócio-demográfico, o Inventário de Burnout (IB) e a Coping Job Scale de Latack (CJS) a uma amostra de 52 médicos (n=140) da ULSM, tendo sido, de seguida, analisados os dados obtidos através do SPSS. Resultados: A taxa de resposta foi de 37,1% (52 em 140 médicos). Destes profissionais, 40 pertenciam ao sexo feminino, 51,9% eram casados e 69.2% eram especialistas em MGF (restantes eram internos de formação específica). O nível total de burnout foi de 3.06±0.64. A Unidade de Saúde Familiar (USF) Progresso apresentou os valores mais elevados (38.20±4.51), seguida da USF Horizonte (32.33±5.83) e da USF Dunas (31.50±5.01). Não se verificaram diferenças entre o estado civil ou o grau académico e nível de burnout ou mecanismos de coping. Não foi encontrado nenhum efeito correlacional significativo entre burnout e mecanismos de coping no geral (r= 0.289, p>005), à excepção da correlação positiva entre o fator controlo e a dimensão “realização pessoal” (r = .407, p < .05). Discussão e Conclusões: No presente estudo, verificou-se um nível total de burnout de 3.06±0.64, valor em concordância com os resultados de burnout obtidos em outros estudos em Portugal na classe médica. Não foi encontrado nenhum efeito correlacional significativo entre burnout e estratégias de coping no geral, apesar de existir uma correlação positiva entre o fator controlo e a dimensão “realização pessoal".

Introducción. La anemia en los niños es un problema de salud pública importante en nuestro país, siendo la anemia por deficiencia de hierro la más frecuente. La frecuencia de anemia en el interior del país es mayor a lo observado en Montevideo. Sin embargo, no existen datos específicos del Departamento de Salto. Objetivos. Determinar la prevalencia de anemia en niños y niñas de 6 a 48 meses que concurren a dos Centros de Atención a la Primera Infancia (CAIF) de la ciudad de Salto y analizar los factores asociados. Metodología. Se determino los niveles de hemoglobina por punción digital en 240 niños de dos CAIF, se relevaron los antecedentes perinatales del carnet del niño/a y datos de ancestralidad por medio de una encuesta a los responsables de los niños. Resultados. La prevalencia de anemia fue de 22,5% en toda la muestra, siendo levemente mayor en el CAIF2 (27,4%) que en el CAIF1(18,4%). La frecuencia de anemia es significativamente menor en los niños que tuvieron lactancia materna exclusiva hasta los 6 meses de edad, mientras que las restantes variables analizadas no presentaron asociación con la anemia. Conclusiones. La disminución observada de la frecuencia de anemia comparada con lo reportado en 2011 sugiere que las políticas de fortificación con hierro de los alimentos y de administración de hierro profiláctico a niños entre 6 y 24 meses ha sido positiva. Adicionalmente, la menor frecuencia de anemia observada en el CAIF1 indica la importancia de la acción coordinada entre los servicios de salud y la sociedad.

Table of contents ORAL ABSTRACTS Symposium 1: Biochemistry, structure and environment of the allergen: what makes a protein an allergen? O1 Two cell-membrane peptidases carrying galactose-alpha-1,3-galactose are implicated in delayed anaphylactic reactions upon pork kidney ingestion in patients with IgE-antibodies to alpha-Gal Christiane Hilger, Kyra Swiontek, Jörg Fischer, François Hentges, Christiane Lehners, Martine Morisset, Bernadette Eberlein, Tilo Biedermann, Markus Ollert O2 Structure solution of Pla l 1 suggests similar folding of Ole e 1-like family members but distinct immunological properties Sabrina Wildner, Teresa Stemeseder, Regina Freier, Peter Briza, Roland Lang, Eva Batanero, Mayte Villalba, Jonas Lidholm, Thomas Hawranek, Fatima Ferreira, Hans Brandstetter, Gabriele Gadermaier Symposium 2: New allergen molecules in the spotlight O3 Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed (Ambrosia artemisiifolia) Philippe Moingeon, Rachel Groeme, Julien Bouley, Véronique Bordas, Maxime Le Mignon, Laetitia Bussières, Aurélie Lautrette, Laurent Mascarell, Vincent Lombardi, Véronique Baron-Bodo, Henri Chabre, Thierry Batard, Emmanuel Nony O4 Production and characterization of polybia paulista recombinant antigen 5: a valuable diagnostic tool Karine Marafigo De Amicis, Alexandra Sayuri Watanabe, Daniele Danella Figo, José Roberto Aparecido Dos Santos-Pinto, Mario Sergio Palma, Fabio Fernandes Morato Castro, Jorge Kalil, Therese Wohlschlager, Peter Briza, Sabrina Wildner, Fatima Ferreira-Briza, Gabriele Gadermaier, Keity Souza Santos Symposium 3: Progress in molecular and cellular diagnosis O5 Basophil activation test with recombinant Pru p 3; identifying genuine peach allergic patients Margaretha Faber, Athina Van Gasse, Vito Sabato, Margo M. Hagendorens, Chris H. Bridts, Luc S. De Clerck, Araceli Diaz Perales, Didier Ebo O6 Nanofluidic technology enables rapid, near-patient quantification of allergen-specific IgE Petra Zavadakova, Aurélie Buchwalder, Fabien Rebeaud, Iwan Märki Symposium 4: Relevance of molecular diagnostics for intervention and treatment O7 Longitudinal analysis of Bet v 1-specific epitope repertoires during birch pollen immunotherapy Barbara Gepp, Nina Lengger, Christian Möbs, Wolfgang Pfützner, Christian Radauer, Barbara Bohle O8 A natural CCD-free tool: is polistes sp. venom suitable for polybia paulista diagnosis and therapy? Karine Marafigo De Amicis, Alexandra Sayuri Watanabe, Clovis Eduardo Galvao, Daniele Danella Figo, Jose Roberto Aparecido Santos-Pinto, Mario Sergio Palma, Fabio Fernandes Morato Castro, Jorge Kalil, Fatima Ferreira, Gabriele Gadermaier, Keity Souza Santos Symposium 5: The advent of molecular allergology in epidemiology O9 Peanut oleosins: from identification to diagnostic testing Christian Schwager, Skadi Kull, Frauke Schocker, Jochen Behrends, Wolf-Meinhard Becker, Uta Jappe O10 Endotypes of oral allergy syndrome in childhood: a molecular diagnostic approach Carla Mastrorilli, Salvatore Tripodi, Carlo Caffarelli, Riccardo Asero, Arianna Dondi, Giampaolo Ricci, Carlotta Povesi Dascola, Elisabetta Calamelli, Andrea Di Rienzo Businco, Annamaria Bianchi, Tullio Frediani, Carmen Verga, Iride Dello Iacono, Diego Peroni, Giuseppe Pingitore, Roberto Bernardini, Paolo Maria Matricardi Symposium 6: Molecular AIT: which approaches will make it to market? O11 Mbc4: an innovative molecule to tackle birch pollen and concomitant food allergies Heidi Hofer, Claudia Asam, Michael Hauser, Peter Briza, Martin Himly, Christof Ebner, Fatima Ferreira O12 Challenges and solutions associated with the production of recombinant Bet v 1 allergen as a therapeutic protein Emmanuel Nony, Maxime Le Mignon, Pierrick Lemoine, Karine Jain, Kathy Abiteboul, Monica Arvidsson, Sabina Rak, Philippe Moingeon Clinical Cases: Breakthroughs and headaches from CRD: interactive session CC1 Anaphylaxis caused by lipid transfer proteins: a complex clinical pattern syndrome Inês Mota, Filipe Benito Garcia, Angela Gaspar, Cristina Arêde, Susana Piedade, Graça Sampaio, Graça Pires, Luís Miguel Borrego, Cristina Santa-Marta, Mário Morais-Almeida CC2 IgE sensitization profile in a patient with asteraceae pollen-exotic fruits association Florin-Dan Popescu, Mariana Vieru, Florin-Adrian Secureanu CC3 Food-dependent: exercise induced anaphylaxis. Which component to blame? Rosa Anita Rodrigues Fernandes, Isabel Carrapatoso, Raquel Gomes, Celso Pereira, Ana Todo-Bom CC4 Anaphylaxis to intravenous iron preparations in a patient that tolerates oral administration María Cecilia Martín Fernández De Basoa, Javier Barrios Regio, Juan De Castro Cordova, Antón Fernández Ferreiro CC5 IgE sensitization pattern in an adult patient with oral allergy syndrome to peanuts and pollinosis from southern Romania Florin-Dan Popescu, Mariana Vieru, Florin-Adrian Secureanu CC6 Evidence of specific IgE to plant-derived cross-reactive carbohydrate determinant in a patient with delayed anaphylaxis to red meat Mariana Vieru, Florin-Dan Popescu, Florin-Adrian Secureanu POSTER PRESENTATIONS Poster Session 1: Molecular allergology and epidemiology P1 Atopic children produce stronger and more frequent IgG responses than non-atopic children: longitudinal data from the German MAS birth cohort Olympia Tsilochristou, Serena Perna, Alina Schwarz, Alexander Rohrbach, Antonio Cappella, Laura Hatzler, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Raffael D’amelio, Ulrich Wahn, Thomas Keil, Susanne Lau, Paolo Maria Matricardi P2 The IgG sensitization profiles against 112 allergenic components support the absence of a protective role of IgG in allergic individuals, outside of the context of SIT Pol André Apoil, Claire Mailhol, Anne Broué-Chabbert, Agnès Juchet, Alain Didier, Elodie Carrer, Thomas Lanot, Antoine Blancher P3 The immune response against the timothy grass pollen allergen Phl p 5 in non-allergic humans Almedina Kurtaj, Christoph Hillebrand, Gerda Fichtinger, Martin Danzer, Christian Gabriel, Theresa Thalhamer, Sandra Scheiblhofer, Josef Thalhamer, Richard Weiss P4 Analyzing the cross-reactivity profile of the major ragweed allergen Amb a 1 Martin Wolf, Michael Hauser, Ulrike Pichler, Teresa Twaroch, Gabriele Gadermaier, Christof Ebner, Hidenori Yokoi, Toshiro Takai, Alain Didierlaurent, Adriano Mari, Peter Briza, Heidrun Behrendt, Angela Neubauer, Frank Stolz, Fátima Ferreira, Michael Wallner P5 LTP (Pru p 3) sensitisation in skin prick test: which means in clinical practice? Sara Carvalho, Tatiana Lourenço, Joana Cosme, Fátima Cabral Duarte, Amélia Spínola Santos, Ana Célia Costa, Manuel Pereira Barbosa P6 IgE profiles, allergen exposure and lifestyle of 501 Austrian pupils: investigation of influences on the development of allergic sensitizations Teresa Stemeseder, Eva Klinglmayr, Bettina Schweidler, Lisa Lueftenegger, Stephanie Moser, Patrick Doppler, Roland Lang, Martin Himly, Gertie J. Oostingh, Arne Bathke, Joerg Zumbach, Thomas Hawranek, Gabriele Gadermaier P7 Molecular profiles of sensitization to perennial inhalant allergens in a middle European region Petr Panzner, Martina Vachova, Tomas Vlas, Marek Maly P8 Evolution of the IgE response to house dust mite allergen molecules in childhood Daniela Posa, Serena Perna, Stephanie Hofmaier, Laura Hatzler, Alexander Rohrbach, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Philippe Stock, Ulrich Wahn, Linus Grabenhenrich, Thomas Keil, Susanne Lau, Kuan-Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Paolo Maria Matricardi P9 Tropomyosin (Pen a1): to include or not to include in skin prick testing? Joana Cosme, Sara Carvalho, Tatiana Lourenço, Amélia Spínola Santos, Manuel Pereira Barbosa Immunoallergy Department - Hospital de Santa Maria – Centro Hospitalar Lisboa Norte, Lisbon, Portugal, Lisbon, Portugal; Immunoallergy Department - Hospital de Santa Maria – Centro Hospitalar Lisboa Norte, Lisbon, Portugal; Faculdade de Medicina de Lisboa, Lisbon, Portugal P10 Component-resolved IgE profiles in Georgian patients Tamar Abramidze, Nino Lomidze, Maia Gotua P11 Cross reactivity between food and pollen allergens in Lithuania according to spIgE evaluation Austeja Dapkeviciute, Ruta Einikyte, Jolita Norkuniene, Laima Skrickiene, Asta Miskiniene, Violeta Kvedariene P12 Distribution of inhalant allergy in the population of Lithuania Ruta Einikyte, Austeja Dapkeviciute, Jolita Norkuniene, Laima Skrickiene, Asta Miskiniene, Violeta Kvedariene Poster Session 2: Allergen molecules: identification, characterization, structure and function P13 Interference of antigen 5-based cross-reactivity in the diagnosis of hymenoptera venom allergy Maximilian Schiener, Bernadette Eberlein, Carmen Moreno-Aguilar, Gunilla Pietsch, Mareike Mc Intyre, Lea Schwarze, Dennis Rußkamp, Tilo Biedermann, Edzard Spillner, Ulf Darsow, Carsten Schmidt-Weber, Markus Ollert, Simon Blank P14 IgE cross-reactivity between European Hymenoptera and Asian hornet (Vespa velutina) venom allergens Cyril Longé, Andrea Brazdova, Jean-Louis Brunet, Claire Schwartz, Bruno Girodet, François Lavaud, Joelle Birnbaum, Nhân Pham Thi, Magalie Duchateau, Julia Chamot-Rooke, Laurence Guilloux, Marie-Ange Selva, Rémy Couderc, Hélène Sénéchal, Jean-Pierre Sutra, Pascal Poncet P15 Carbohydrate composition of house dust mite extracts and major group 1 and group 2 allergens Steffen Augustin, Linda Pump, Martin Wald, Thomas Eichhorn, Frank Fischer, Christoph Willers P16 Specificity of monoclonal antibodies against cross-reactive carbohydrate determinants Michaela Miehe, Melanie Plum, Sara Wolf, Frederic Jabs, Tim Raiber, Frank Bantleon, Henning Seismann, Thilo Jakob, Edzard Spillner P17 Red meat allergic patients have a selective IgE response to the a-Gal glycan Danijela Apostolovic, Anh Thu Tran, Sara Sanchez-Vidaurre, Tanja Cirkovic Velickovic, Maria Starkhammar, Carl Hamsten, Marianne Van Hage P18 Specificity of non-specific lipid transfer proteins and influence of the ligands on their three-dimensional structure Pawel Dubiela, Piotr Humeniuk, Sabine Pfeifer, Merima Bublin, Tomasz Borowski, Karin Hoffmann-Sommergruber P19 Real-time PCR analysis of Pru av 1 and Pru av 3 allergens Martie C.M. Verschuren, Shanna Bastiaan-Net, Defien Depoortere, Kay Foetisch, Stephan Scheurer, Harry J Wichers, Theo Noij P20 Specificity of anti-Pru av 1 antibodies for the detection of Pru av 1 isoallergens Martie C.M. Verschuren, Shanna Bastiaan-Net, Nikki M.E. Van Uden, Karel Vandenberghe, Kay Foetisch, Stephan Scheurer, Harry J. Wichers H.J., Theo H.M. Noij P21 Enhancing recombinant production yield of Bet v 1 through codon usage harmonization Anargyros Roulias, Maria Alejandra Parigiani, Heidi Hofer, Claudia Asam, Christof Ebner, Fátima Ferreira P22 Structural and dynamic insights into the world of PR-10 allergens Linda Ahammer, Sarina Grutsch, Martin Tollinger Poster Session 3: Allergen molecules: identification, characterization, structure and function P23 Purification of polcalcin from different pollen allergenic sources by antibody-affinity chromatography Raquel Moya, Mª Angeles López-Matas, Raquel Reyes, Jerónimo Carnés P24 Variations of wheat allergens in cultivars measured through a targeted quantitative mass spectrometry approach Colette Larré, Hélène Rogniaux, Roberta Lupi, Sandra Denery-Papini P25 Art v 1, Amb a 4 and Par h 1 defensin-like proteins share similar structural features but distinct immunological and allergenic properties Isabel Maria Pablos, Stephanie Eichhorn, Yoan Machado, Peter Briza, Christof Ebner, Jung-Won Park, Alain Didierlaurent, Naveen Arora, Stefan Vieths, Gabriele Gadermaier, Fatima Ferreira P26 Homogeneity or diversity of IgE-binding proteins in wheat dependant exercise induced anaphylaxis? Sandra Denery-Papini, Charlene Tanaka, Florence Pineau, Roberta Lupi, Martine Drouet, Etienne Beaudouin, Martine Morisset, Susan Altenbach P27 Deciphering the role of disulfide bonds and of repetitive epitopes in immunoglobulin E binding to wheat gliadins Sandra Denery-Papini, Hamza Mameri, Chantal Brossard, Roberta Lupi, Florence Pineau, Jean Charles Gaudin, Denise Anne Moneret-Vautrin, Etienne Beaudouin, Evelyne Paty, Martine Drouet, Olivier Tranquet, Colette Larré P28 Assessment of the allergenicity of soluble fractions from bread and durum wheats genotypes Roberta Lupi, Stefania Masci, Olivier Tranquet, Denise-Anne Moneret-Vautrin, Sandra Denery-Papini, Colette Larré P29 Isolation and characterization of Ara h 12 and Ara h 13: defensins, a novel class of peanut allergens Skadi Kull, Arnd Petersen, Marisa Böttger, Sandra Rennert, Wolf-Meinhard Becker, Susanne Krause, Martin Ernst, Thomas Gutsmann, Johann Bauer, Buko Lindner, Uta Jappe P30 Allergenicity attributes of different peanut market types Stef Koppelman, Shyamali Jayasena, Dion Luykx, Erik Schepens, Danijela Apostolovic, Govardus De Jong, Tom Isleib, Julie Nordlee, Joe Baumert, Steve Taylor, Soheila Maleki P31 The impact of peanut lipids on Ara h 1-induced immune responses in monocytes-derived dendritic cells Chiara Palladino, Barbara Gepp, Sofía Sirvent, Alba Angelina, Merima Bublin, Christian Radauer, Nina Lengger, Thomas Eiwegger, Oscar Palomares, Heimo Breiteneder P32 Compared allergenicity of native and thermally aggregated ovalbumin as large agglomerated particles Mathilde Claude, Roberta Lupi, Grégory Bouchaud, Marie Bodinier, Chantal Brossard, Sandra Denery-Papini P33 Simulation of the gastrointestinal digestion of the hazelnut allergens Cor a 9 and Cor a 11 by an in-vitro model and characterisation of peptidic products including epitopes by HPLC-MS/MS Robin Korte, Julia Bräcker, Jens Brockmeyer P34 Analysis of distribution of rice allergens in brown rice grain and allergenicity of the products containing rice bran Rie Satoh, Reiko Teshima Poster Session 4: Molecular approaches in AIT P35 Production of a recombinant hypoallergenic variant of the major peanut allergen Ara h 2 for allergen-specific immunotherapy Angelika Tscheppe, Dieter Palmberger, Merima Bublin, Christian Radauer, Chiara Palladino, Barbara Gepp, Nina Lengger, Reingard Grabherr, Heimo Breiteneder P36 Mutagenesis of amino acids critical for calcium-binding leads to the generation of a hypoallergenic Phl p 7 variant Marianne Raith, Linda Sonnleitner, Doris Zach, Konrad Woroszylo, Margit Focke-Tejkl, Herbert Wank, Thorsten Graf, Annette Kuehn, Ines Swoboda P37 Are birch pollen allergen immunotherapy induced blocking antibodies protective for cross-reactive allergens? Claudia Asam, Sara Huber, Heidi Hofer, Roland Lang, Thomas Hawranek, Fátima Ferreira, Michael Wallner P38 High success of 58 subcutaneous immunotherapy for pets allergy in a polyallergic cohort of patients: a component resolved individually adapted treatment (CRIAT) Fabienne Gay-Crosier P39 Neutrophils are potential antigen presenting cells in IgE- mediated allergy Dominika Polak, Birgit Nagl, Claudia Kitzmüller, Barbara Bohle P40 Characterization of allergen-specific CD8+ T cells in type I allergy Nazanin Samadi, Claudia Kitzmüller, Rene Geyeregger, Barbara Bohle, Beatrice Jahn-Schmid Poster Session 5: Molecular and cellular diagnostic tests P41 Nanofluidic-based biosensors allow quantification of total circulating IgE from a drop of blood in 5 minutes Aurélie Buchwalder, Ariel Gomez, Fabien Rebeaud, Iwan Märki P42 Allergen microarray for the analysis of serum IgE binding profile and allergenic activity Jaana Haka, Liisa Hattara, Marika Heikkinen, Merja H Niemi, Juha Rouvinen, Petri Saviranta, Pekka Mattila, Kristiina Takkinen, Marja-Leena Laukkanen P43 Generation of a well-characterized panel of periplaneta americana allergens for component resolved diagnosis Stephanie Eichhorn, Isabel Pablos, Bianca Kastner, Bettina Schweidler, Sabrina Wildner, Peter Briza, Jung-Won Park, Naveen Arora, Stefan Vieths, Gabriele Gadermaier, Fatima Ferreira P44 Improved diagnostic sensitivity of recombinant Api m 1 and Ves v 5 in diagnosis of Hymenoptera venom allergy Mira Silar, Julij Selb, Rok Kogovsek, Mitja Kosnik, Peter Korosec P45 Added value of biomarkers of primary sensitization and cross-reactivity in patients with hymenoptera venom allergy Leticia Pestana, Alcinda Campos Melo, Ana Mendes, Maria Elisa Pedro, Manuel Pereira Barbosa, Maria Conceição Pereira Santos P46 Cosensitization to Alt a 1 and Act d 2: more than a fortuitous association? Françoise Bienvenu, Claire Goursaud, Lorna Garnier, Sandrine Jacquenet, Michaël Degaud, Sébastien Viel, Annick Barre, Pierre Rougé, Jacques Bienvenu, Joana Vitte P47 Molecular diagnosis for peanut allergy: ALFA method performs as well as established methods for Ara h 1, Ara h 2, Ara h 6, Ara h 9 and CCD Amel Bensalah, Isabelle Cleach, Laurent Mousseau, Chantal Agabriel, Valérie Liabeuf, Joëlle Birnbaum, Jean-Louis Mège, Joana Vitte P48 Evaluation of a food challenge service in relation to specific IgE to molecular components in children with suspected peanut allergy James Gardner, Minal Gandhi, Harsha Kariyawasam, Giuseppina Rotiroti P49 Component resolved diagnosis in cereal allergy Isabel Carrapatoso, Celso Pereira, Frederico Regateiro, Emília Faria, Ana Todo-Bom Poster Session 6: Molecular diagnosis in prevention and therapy P50 Pretreatment molecular sensitizations determine the sIgG4 induction during the updosing of SCIT and may be useful to identify clinically relevant additional sensitizations Johannes Martin Schmid, Ronald Dahl, Hans Juergen Hoffmann P51 Usefulness of recombinant latex allergens in immunotherapy’s decision and follow-up Inês Mota, Filipe Benito Garcia, Angela Gaspar, Mário Morais-Almeida P52 Omega-5-gliadin in the diagnosis of wheat-dependent anaphylaxis induced by ibuprofen but not by exercise Joana Cosme, Letícia Pestana, Amélia Spínola Santos, Manuel Pereira Barbosa P53 Food dependent exercise-induced anaphylaxis: a component-resolved and in vitro depletion approach to access IgE cross-reactivity Diana Silva, Teresa Vieira, Ana Maria Pereira, André Moreira, Luís Delgado P54 Olive pollen allergens: what are we missing? Sara Prates, Cátia Alves, Elena Finelli, Paula Leiria Pinto P55 Purified Alt a 1 extract in Alternaria alternata allergy diagnosis Bárbara Kong Cardoso, Cíntia Cruz, Filipa Semedo, Elza Tomaz, Filipe Inácio P56 Use of specific IgE Bos d8 (casein) to aid early introduction of dietary baked milk in children with cows’ milk allergy James Gardner, Santanu Maity, Giuseppina Rotiroti, Minal Gandhi P57 Molecular characterisation and immunoreactivity of a peanut ingredient for use in oral food challenges Ivona Baricevic-Jones, Justin T. Marsh, Phil E. Johnson, Anuradha Balasundaram, Anya-May Hope, Aafke Taekema, Angela Simpson, Aida Semic-Jusufagic, E.N. Clare Mills P58 Specific IgE to recombinant allergens of hazelnut and oral food challenge in children Gourdon Dubois Nelly, Sellam Laetitia, Pereira Bruno, Michaud Elodie, Messaoudi Khaled, Evrard Bertrand, Fauquert Jean-Luc Poster session 7/8: miscellaneous P59 What defines a protein as an allergen? A discussion of sources and sufficiency Richard E. Goodman P60 Cat allergy: relationship between clinical and molecular diagnostic María Cecilia Martín Fernández De Basoa, Antón Fernández Ferreiro, Elena Rodríguez Plata P61 Anaphylaxis to rabbit: the cat came in last Luis Amaral, Borja Bartolomé, Alice Coimbra, Jose L Placido P62 Dog allergy: relationship between clinical and molecular diagnostic María Cecilia Martín Fernández De Basoa, Antón Fernández Ferreiro, Elena Rodríguez Plata P63 Correlation of serum timothy grass-pollen specific IgE levels determined by two immunoblot test systems Mariana Vieru, Florin-Dan Popescu, Florin-Adrian Secureanu, Carmen Saviana Ganea P64 Development of oral food challenge formulations for diagnosis of fish allergy using powdered fish ingredients Carol Ann Costello, Ivona Baricevic-Jones, Martin Sorensen, Clare Mills, Adrian Rogers, Aage Otherhals P65 Fish and peanut allergens interact with plasma membranes of intestinal and bronchial epithelial cells and induce differential gene expression of cytokines and chemokines Tanja Kalic, Isabella Ellinger, Chiara Palladino, Barbara Gepp, Eva Waltl, Verena Niederberger-Leppin, Heimo Breiteneder P66 Interleukin 4 affects fat tissue metabolism and expression of pro-inflammatory factors in isolated rat adipocytes Dawid Szczepankiewicz, Ewa Pruszynska-Oszmalek, Marek Skrzypski, Krzysztof W. Nowak, Aleksandra Szczepankiewicz P67 Ozone induced airway hyperreactivity in PD-L2−/− mice model Gwang-Cheon Jang P68 Thymic stromal lymphopoietin (TSLP) and its receptor as targets for the development of anti-inflammatory inhibitory agents Iva Markovic, Andreas Borowski, Tina Vetter, Andreas Wohlmann, Michael Kuepper, Karlheinz Friedrich P69 The mononuclear phagocyte system in experimentally-induced allergic rhinitis Ibon Eguiluz Gracia, Anthony Bosco, Ralph Dollner, Guro Reinholt Melum, Anya C Jones, Maria Lexberg, Patrick G Holt, Espen Sønderaal Bækkevold, Frode Lars Jahnsen P70 Expression of histamine metabolizing enzymes is increased in allergic children Aleksandra Szczepankiewicz, Paulina Sobkowiak, Marta Rachel, Beata Narozna, Dorota Jenerowicz, Witold Swiatowy, Anna Breborowicz P71 Modifying the glycosylation of human IgE towards oligomannosidic structures does not affect its biological activity Melanie Plum, Sara Wolf, Frank Bantleon, Henning Seismann, Frederic Jabs, Michaela Miehe, Thilo Jakob, Edzard Spillner P72 Flying Labs: an educational initiative to transfer allergy research into high-school settings Michael Wallner, Heidi Hofer, Fatima Ferreira, Reinhard Nestelbacher P73 Clinical significance of antihistamines and Kujin, an anti-allergic Kampo medicine Hiroyuki Fukui

O aquífero associado à Formação Alter do Chão (FAC) — subsistema aquífero Alter do Chão (AAC) — é o mais conhecido da região amazônica. Estudos recentes indicam sua possível conexão com o subsistema aquífero cretáceo Tikuna, que possui continuidade por todas as bacias amazônicas, ambos integrando o grande Sistema Aquífero Amazônico (SAA). O objetivo do presente trabalho foi o aperfeiçoamento do modelo conceitual de funcionamento do AAC a oeste de Manaus. A metodologia incluiu medidas do nível d’água, de parâmetros físico-químicos in situ em poços e amostragem para análises químicas e isotópicas das águas subterrâneas. Realizou-se o levantamento das características geológicas e limites do aquífero, baseado em dados de poços de petróleo e de abastecimento de água existentes. Os resultados revelaram que as águas do AAC a oeste de Manaus possuem duas origens principais: 1) origem meteórica, pouco mineralizadas, situadas nas camadas aquíferas superiores, em um sistema livre, com recarga local; 2) origem remota, mais mineralizadas, com maior tempo de residência no aquífero, armazenadas nas camadas mais profundas em um sistema provavelmente confinado, originadas possivelmente em áreas de recarga do bordo oeste do SAA. As análises isotópicas calibradas sugerem uma idade média de 21.380 anos para águas subterrâneas mais mineralizadas e profundas dos municípios de Manacapuru e Careiro. Essa água é, provavelmente, resultado de uma mescla de águas mais antigas com águas de recarga mais recente. Por conta dessa diferenciação hidrogeoquímica, correlacionada com a geologia local, o AAC foi subdividido em uma porção superior, livre e menos mineralizada, e outra inferior, mais antiga e mineralizada, ambas misturadas a leste do Arco de Purus, com progressiva diluição pela chuva.

Resumo Objetivo: o presente estudo descreve a utilização do Photovoice para aprofundar o conceito de família em estudantes de enfermagem. Metodologia: é um estudo de investigação ação participativa, em que participaram 33 estudantes de enfermagem. A coleta de dados utilizou o Photovoice para definir o conceito de família; as sessões foram gravadas e transcritas. Para a análise, recorreu-se à triangulação de dados com as fotografias com recurso ao software Atlas-ti versão 8.0. Resultados: quatro temas principais emergiram das fotografias e das gravações: Conceito de família na perspetiva do estudante, A minha família, Funções da família, O que vejo nesta fotografia. Conclusão: o estudo contribuiu para a divulgação do photovoice, uma ferramenta ainda pouco utilizada em estudos em enfermagem. Além disso, promoveu a importância da família no cuidar em enfermagem, através da conceptualização do que é família.

Information on a population’s dietary intake enables the identification of individuals who are at nutritional risk. The objective of the present study was to identify and analyze existing dietary assessment instruments in school-age children. An integrative review was carried out from 2010 to 2016. For analysis, nine articles were selected according to established inclusion criteria. These enabled the organization of the results into two thematic areas: dietary assessment instruments and limitations in the use of instruments. The most used instrument was the 24-hour dietary recall. The results showed the need for more comprehensive instruments in order to deal with limitations of current instruments in use.

A aposta crescente a que se tem vindo a assistir recentemente na área das Línguas Clássicas, no Ensino Básico e Secundário em Portugal, requer uma revisão dos conceitos da sua didática específica, que traduza uma síntese entre as práticas do passado e as novas tendências dos métodos pedagógicos. A escola do século XXI necessita de entender o Latim como uma disciplina dinâmica, cuja metodologia de ensino se aproxime da metodologia ativa das línguas modernas, valorizando a oralidade, a compreensão textual, a aquisição de vocabulário e, sobretudo, a consciencialização de que esta disciplina representa um pilar imprescindível para muitas áreas de estudo, contribuindo para a formação integral e integradora dos alunos. A importância que o Latim assume nas Humanidades requer que se atualizem os métodos, nomeadamente a abordagem da gramática e a integração da cultura. Desta forma, o presente artigo pretende constituir uma reflexão acerca das perspetivas atuais do ensino das Línguas Clássicas, analisando, em retrospetiva, algumas opiniões de especialistas e acrescentando algumas sugestões metodológicas.

Este estudo se propôs investigar a prática da Educação Física nas escolas sob a ótica da Teoria das Representações Sociais na busca da identificação da ancoragem das representações sociais nos Modelos Teóricos desta disciplina. Baseado em estudo anterior onde a representação social identificada está fundamentada nos elementos: LÚDICO e DESENVOLVIMENTO como elementos centrais, os resultados obtidos no atual estudo indicam que a Representação Social identificada está ancorada nas abordagens: Desenvolvimentista e Psicomotora. Podemos sugerir ainda que a prática da Educação Física Escolar está a serviço do DESENVOLVIMENTO e este desenvolvimento é promovido principalmente por meio de estratégias LÚDICAS e ESPORTIVAS.

OBJETIVO: Comparar os sintomas de depressão e o comportamento sexual de gestantes com histórico de aborto espontâneo de repetição (AER) com gestantes que não vivenciaram AER.MÉTODOS: Trata-se de um estudo prospectivo caso-controle. O primeiro grupo é formado por mulheres que apresentaram AER e o segundo, por primigestas. Foram utilizados o Beck Depression Inventory (BDI), o Female Sexual Function Index (FSFI) e mais um questionário desenvolvido pelos autores sobre aspectos emocionais decorrentes da relação sexual na gravidez. Para comparar as variáveis quantitativas, com distribuição normal, utilizou-se o teste t de Student, e as variáveis categóricas foram comparadas com base no teste do χ2 ou no teste exato de Fisher. A significância estatística foi considerada para valores de p

Pollen released by allergenic members of the botanically unrelated families of Asteraceae and Cupressaceae represent potent elicitors of respiratory allergies in regions where these plants are present. As main allergen sources the Asteraceae species ragweed and mugwort, as well as the Cupressaceae species, cypress, mountain cedar, and Japanese cedar have been identified. The major allergens of all species belong to the pectate lyase enzyme family. Thus, we thought to investigate cross-reactivity pattern as well as sensitization capacities of pectate lyase pollen allergens in cohorts from distinct geographic regions.The clinically relevant pectate lyase pollen allergens Amb a 1, Art v 6, Cup a 1, Jun a 1, and Cry j 1 were purified from aqueous pollen extracts, and patients' sensitization pattern of cohorts from Austria, Canada, Italy, and Japan were determined by IgE ELISA and cross-inhibition experiments. Moreover, we performed microarray experiments and established a mouse model of sensitization.In ELISA and ELISA inhibition experiments specific sensitization pattern were discovered for each geographic region, which reflected the natural allergen exposure of the patients. We found significant cross-reactivity within Asteraceae and Cupressaceae pectate lyase pollen allergens, which was however limited between the orders. Animal experiments showed that immunization with Asteraceae allergens mainly induced antibodies reactive within the order, the same was observed for the Cupressaceae allergens. Cross-reactivity between orders was minimal. Moreover, Amb a 1, Art v 6, and Cry j 1 showed in general higher immunogenicity.We could cluster pectate lyase allergens in four categories, Amb a 1, Art v 6, Cup a 1/Jun a 1, and Cry j 1, respectively, at which each category has the potential to sensitize predisposed individuals. The sensitization pattern of different cohorts correlated with pollen exposure, which should be considered for future allergy diagnosis and therapy.

The syndrome of bilateral basal ganglia lesions in diabetic uremic patients is a rare disorder mostly reported in Asians. There are few reports of the syndrome in Caucasians. It manifests as an acute hyperkinetic or hypokinetic extrapyramidal disorder in association with uniform neuroimaging findings of bilateral symmetrical basal ganglia changes in diabetics undergoing hemodialysis. Its pathophysiology remains largely unknown. Thus, we report a typical case of the syndrome in a Caucasian patient who developed an acute and reversible akinetic rigid parkinsonism secondary to bilateral basal ganglia lesions.

Allergy prevalence has increased in industrialized countries. One contributing factor could be pollution, which can cause nitration of allergens exogenously (in the air) or endogenously (in inflamed lung tissue). We investigated the impact of nitration on both the structural and immunological behavior of the major birch pollen allergen Bet v 1.0101 to determine whether nitration might be a factor in the increased incidence of allergy. Bet v 1.0101 was nitrated with tetranitromethane. Immune effects were assessed by measuring the proliferation of specific T-cell lines (TCLs) upon stimulation with different concentrations of nitrated and unmodified allergen, and by measurement of cytokine release of monocyte-derived dendritic cells (moDCs) and primary DCs (primDCs) stimulated with nitrated versus unmodified allergen. HPLC-MS, crystallography, gel electrophoresis, amino acid analysis, size exclusion chromatography and molecular dynamics simulation were performed to characterize structural changes after nitration of the allergen. The proliferation of specific TCLs was higher upon stimulation with the nitrated allergen in comparison to the unmodified allergen. An important structural consequence of nitration was oligomerization. Moreover, analysis of the crystal structure of nitrated Bet v 1.0101 showed that amino acid residue Y83, located in the hydrophobic cavity, was nitrated to 100%. Both moDCs and primDCs showed decreased production of TH1-priming cytokines, thus favoring a TH2 response. These results implicate that nitration of Bet v 1.0101 might be a contributing factor to the observed increase in birch pollen allergy, and emphasize the importance of protein modifications in understanding the molecular basis of allergenicity.

The authors studied the relative prevalence of erythroid cytoskeletal protein defects and their relationship with the clinical course of Hereditary Spherocytosis (HS) in 39 Portuguese patients of North of Portugal (25 families). This study showed that, in the North of Portugal, HS is primarily due to anquirin deficiency (72%), followed by band 3 (20%). These findings are similar to the published data in other Caucasian populations. Anquirin primary defects have been difficult to diagnose before splenectomy, due to high reticulocytes counts.

The growing number of novel candidate molecules for the treatment of allergic diseases imposed a dramatic increase in the demand for animal experiments to select immunogenic vaccines, a pre-requisite for efficacy. Because no in vitro methods to predict the immunogenicity of a protein are currently available, we developed an in vitro assay that exploits the link between a protein's immunogenicity and its susceptibility to endolysosomal proteolysis.We compared protein composition and proteolytic activity of endolysosomal fractions isolated from murine bone marrow- and human blood- derived dendritic cells, and from the dendritic cell line JAWS II. Three groups of structurally related antigen variants differing in their ability to elicit immune responses in vivo (Bet v 1.0101 and Bet v 1.0401, RNases A and S, holo- and apo-HRP) were subjected to in vitro simulated endolysosomal degradation. Kinetics and patterns of generated proteolytic peptides were evaluated by gel electrophoresis and mass spectrometry.Antigens displaying weak capacity of T cell priming in vivo were highly susceptible to endolysosomal proteases in vitro. As proteolytic composition, activity, and specificity of endolysosomal fractions derived from human and murine dendritic cells were comparable, the JAWS II cell line could be used as a substitute for freshly isolated human or murine cells in in vitro degradation assays.Endolysosomal fractions prepared from the JAWS II cell line provide a reliable tool for in vitro estimation of protein immunogenicity. The rapid and simple assay described here is very useful to study the immunogenic properties of a protein, and can help to replace, reduce, and refine animal experiments in allergy research and vaccine development in general.

A sobrecarga do cuidador principal constitui um problema importante, o que desperta interesse não só de trabalhos de investigação mas também de instrumentos de avaliação, como a Zarit Burden Interview que avalia a sobrecarga do cuidador em diferentes níveis. Objectivo: Validação da escala de Zarit (Zarit Burden Interview) para os cuidadores de doentes paliativos em âmbito domiciliário, para a realidade portuguesa. Processo metodológico: Estudo metodológico de natureza quantitativa, aplicado a uma amostra de 104 cuidadores de doentes em cuidados paliativos domiciliários, nas diversas equipas existentes no país. Este estudo descreve a construção e as características psicométricas do instrumento. Resultados: A avaliação da consistência interna foi efectuada através do alfa de Cronbach, sendo o valor obtido de 0,884. O total da média dos scores totais da escala de Zarit neste grupo de cuidadores foi de 37,26, o que indica sobrecarga moderada. Da análise estatística do instrumento evidenciam-se cinco factores multidimensionais de sobrecarga. Conclusão: A escala de sobrecarga “The Zarit Burden Interview” tem boas características psicométricas, pelo que se aconselha a sua aplicação a cuidadores de doentes em cuidados paliativos domiciliários na população portuguesa.

Procedeu-se no estudo a determinação de atributos e a concepção de um modelo hidrogeológico conceitual do aqüífero Carbonático raso em áreas da Bacia Potiguar. Desta forma se tem elementos que podem fundamentar a compreensão, análise e influência de controles hidrogeológicos que justifiquem e garantam as condições de background das substâncias dissolvidas nas águas subterrâneas, e as prováveis variações nos seus teores normais de ocorrência no referido aqüífero. Nesse entendimento os fatores hidrogeológicos determinam possibilidades de que os teores de background possam resultar de processos geoquímicos de interação água-rocha ligados ao fluxo subterrâneo, bem como por influência de variações hidroestratigráficas, padrões estruturais, climáticas, processos de recarga, mistura de águas, e proximidades do aqüífero raso com linha de costa oceânica.

Abstract Background Plasmodium falciparum malaria remains a leading health problem in Africa and its control is seriously challenged by drug resistance. Although resistance to the sulphadoxine-pyrimethamine (SP) is widespread, this combination remains an important component of malaria control programmes as intermittent preventive therapy (IPT) for pregnant women and children. In Angola, resistance patterns have been poorly characterized, and IPT has been employed for pregnant women since 2006. The aim of this study was to assess the prevalence of key antifolate resistance mediating polymorphisms in the pfdhfr and pfdhps genes in P. falciparum samples from Angola. Methods Plasmodium falciparum samples collected in Luanda, in 2007, were genotyped by amplification and DNA forward and reverse sequencing of the pfdhfr and pfdhps genes. Results The most prevalent polymorphisms identified were pfdhfr 108N (100%), 51I (93%), 59R (57%) and pfdhps 437G (93%). Resistance-mediating polymorphisms in pfdhps less commonly observed in West Africa were also identified (540E in 10%, 581G in 7% of samples). Conclusion This study documents an important prevalence of 4 P. falciparum polymorphisms that predicts an antifolate resistance in Luanda. Further, some samples presented additional mutations associated to high-level resistance. These results suggest that the use of SP for IPT may no longer be warranted in Angola.