137 results on '"Desveaux, Darrell"'
Search Results
2. Cooperative virulence via the collective action of secreted pathogen effectors
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Ruiz-Bedoya, Tatiana, Wang, Pauline W., Desveaux, Darrell, and Guttman, David S.
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- 2023
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3. Towards integrative plant pathology
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Ruiz-Bedoya, Tatiana, McTavish, Kathryn J., Av-Shalom, Tamar V., Desveaux, Darrell, and Guttman, David S.
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- 2023
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4. The small molecule Zaractin activates ZAR1-mediated immunity in Arabidopsis
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Seto, Derek, Khan, Madiha, Bastedo, D. Patrick, Martel, Alexandre, Vo, Trinh, Guttman, David, Subramaniam, Rajagopal, and Desveaux, Darrell
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- 2021
5. The Arabidopsis effector-triggered immunity landscape is conserved in oilseed crops
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Breit-McNally, Clare, Desveaux, Darrell, and Guttman, David S.
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- 2022
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6. The Pseudomonas syringae Effector AvrRpt2 Cleaves Its C-Terminally Acylated Target, RIN4, from Arabidopsis Membranes to Block RPM1 Activation
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Kim, Han-Suk, Desveaux, Darrell, Singer, Alex U., Patel, Priyesh, Sondek, John, Dangl, Jeffery L., and Anderson, Kathryn V.
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- 2005
7. The Arabidopsis NPR1 Disease Resistance Protein Is a Novel Cofactor That Confers Redox Regulation of DNA Binding Activity to the Basic Domain/Leucine Zipper Transcription Factor TGA1
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Després, Charles, Chubak, Catherine, Rochon, Amanda, Clark, Rena, Bethune, Terry, Desveaux, Darrell, and Fobert, Pierre R.
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- 2003
8. Pseudomonas syringae coffee blight is associated with the horizontal transfer of plasmid‐encoded type III effectors.
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McTavish, Kathryn J., Almeida, Renan N. D., Tersigni, Jonathan, Raimundi, Melina K., Gong, Yunchen, Wang, Pauline W., Gontijo, Guilherme F., de Souza, Ricardo M., de Resende, Mario L. V., Desveaux, Darrell, and Guttman, David S.
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PSEUDOMONAS syringae ,GENOME-wide association studies ,COFFEE ,PLANT diseases ,COFFEE plantations ,CANKER (Plant disease) - Abstract
Summary: The emergence of new pathogens is an ongoing threat to human health and agriculture. While zoonotic spillovers received considerable attention, the emergence of crop diseases is less well studied. Here, we identify genomic factors associated with the emergence of Pseudomonas syringae bacterial blight of coffee.Fifty‐three P. syringae strains from diseased Brazilian coffee plants were sequenced. Comparative and evolutionary analyses were used to identify loci associated with coffee blight. Growth and symptomology assays were performed to validate the findings.Coffee isolates clustered in three lineages, including primary phylogroups PG3 and PG4, and secondary phylogroup PG11. Genome‐wide association study of the primary PG strains identified 37 loci, including five effectors, most of which were encoded on a plasmid unique to the PG3 and PG4 coffee strains. Evolutionary analyses support the emergence of coffee blight in PG4 when the coffee‐associated plasmid and associated effectors derived from a divergent plasmid carried by strains associated with other hosts. This plasmid was only recently transferred into PG3. Natural diversity and CRISPR‐Cas9 plasmid curing were used to show that strains with the coffee‐associated plasmid grow to higher densities and cause more severe disease symptoms in coffee.This work identifies possible evolutionary mechanisms underlying the emergence of a new lineage of coffee pathogens. [ABSTRACT FROM AUTHOR]
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- 2024
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9. PBF-2 Is a Novel Single-Stranded DNA Binding Factor Implicated in PR-10a Gene Activation in Potato
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Desveaux, Darrell, Després, Charles, Joyeux, Alexandre, Subramaniam, Rajagopal, and Brisson, Normand
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- 2000
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10. Fucosyltransferase and the Biosynthesis of Storage and Structural Xyloglucan in Developing Nasturtium Fruits
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Desveaux, Darrell, Faik, Ahmed, and Maclachlan, Gordon
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- 1998
11. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases
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Smakowska-Luzan, Elwira, Mott, G. Adam, Parys, Katarzyna, Stegmann, Martin, Howton, Timothy C, Layeghifard, Mehdi, Neuhold, Jana, Lehner, Anita, Kong, Jixiang, Grnwald, Karin, Weinberger, Natascha, Satbhai, Santosh B., Mayer, Dominik, Busch, Wolfgang, Madalinski, Mathias, Stolt-Bergner, Peggy, Provart, Nicholas J., Mukhtar, M. Shahid, Zipfel, Cyril, Desveaux, Darrell, Guttman, David S., and Belkhadir, Youssef
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Arabidopsis thaliana -- Physiological aspects ,Extracellular matrix -- Physiological aspects ,Phosphotransferases -- Physiological aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Elwira Smakowska-Luzan [1]; G. Adam Mott [2]; Katarzyna Parys [1]; Martin Stegmann [3]; Timothy C Howton [4]; Mehdi Layeghifard [2]; Jana Neuhold [5]; Anita Lehner [5]; Jixiang Kong [1]; [...]
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- 2018
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12. The ERF11 Transcription Factor Promotes Internode Elongation by Activating Gibberellin Biosynthesis and Signaling
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Zhou, Xin, Zhang, Zhong-Lin, Park, Jeongmoo, Tyler, Ludmila, Yusuke, Jikumaru, Qiu, Kai, Nam, Edward A., Lumba, Shelley, Desveaux, Darrell, McCourt, Peter, Kamiya, Yuji, and Sun, Tai-ping
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- 2016
13. The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a
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Lewis, Jennifer D., Lee, Amy Huei-Yi, Hassan, Jana A., Wan, Janet, Hurley, Brenden, Jhingree, Jacquelyn R., Wang, Pauline W., Lo, Timothy, Youn, Ji-Young, Guttman, David S., and Desveaux, Darrell
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- 2013
14. Arabidopsis ETHYLENE RESPONSE FACTOR 8 (ERF8) has dual functions in ABA signaling and immunity
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Cao, Feng Yi, DeFalco, Thomas A., Moeder, Wolfgang, Li, Bo, Gong, Yunchen, Liu, Xiao-Min, Taniguchi, Masatoshi, Lumba, Shelley, Toh, Shigeo, Shan, Libo, Ellis, Brian, Desveaux, Darrell, and Yoshioka, Keiko
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- 2018
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15. In planta proximity dependent biotin identification (BioID)
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Khan, Madiha, Youn, Ji-Young, Gingras, Anne-Claude, Subramaniam, Rajagopal, and Desveaux, Darrell
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- 2018
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16. The Type III Effector $HopF2_{Pto} $ Targets Arabidopsis RIN4 Protein to Promote Pseudomonas Syringae Virulence
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Wilton, Mike, Subramaniam, Rajagopal, Elmore, James, Felsensteiner, Corinna, Coaker, Gitta, Desveaux, Darrell, and Dangl, Jeffery L.
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- 2010
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17. Plant Chemical Genetics
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McCourt, Peter and Desveaux, Darrell
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- 2010
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18. Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins
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Park, Sang-Youl, Fung, Pauline, Nishimura, Noriyuki, Jensen, Davin R., Fujii, Hiroaki, Zhao, Yang, Lumba, Shelley, Santiago, Julia, Rodrigues, Americo, Chow, Tsz-fung F., Alfred, Simon E., Bonetta, Dario, Finkelstein, Ruth, Provart, Nicholas J., Desveaux, Darrell, Rodriguez, Pedro L., McCourt, Peter, Zhu, Jian-Kang, Schroeder, Julian I., Volkman, Brian F., and Cutler, Sean R.
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- 2009
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19. ZAR1: Guardian of plant kinases.
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Breit-McNally, Clare, Laflamme, Bradley, Singh, Racquel A., Desveaux, Darrell, and Guttman, David S.
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RECEPTOR-like kinases ,PROTEIN kinases ,DISEASE resistance of plants ,PROTEIN folding - Abstract
A key facet of innate immunity in plants entails the recognition of pathogen "effector" virulence proteins by host Nucleotide-Binding Leucine-Rich Repeat Receptors (NLRs). Among characterized NLRs, the broadly conserved ZAR1 NLR is particularly remarkable due to its capacity to recognize at least six distinct families of effectors from at least two bacterial genera. This expanded recognition spectrum is conferred through interactions between ZAR1 and a dynamic network of two families of Receptor-Like Cytoplasmic Kinases (RLCKs): ZED1-Related Kinases (ZRKs) and PBS1-Like Kinases (PBLs). In this review, we survey the history of functional studies on ZAR1, with an emphasis on how the ZAR1-RLCK network functions to trap diverse effectors. We discuss 1) the dynamics of the ZAR1-associated RLCK network; 2) the specificity between ZRKs and PBLs; and 3) the specificity between effectors and the RLCK network. We posit that the shared protein fold of kinases and the switch-like properties of their interactions make them ideal effector sensors, enabling ZAR1 to act as a broad spectrum guardian of host kinases. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Diversity, Evolution, and Function of Pseudomonas syringae Effectoromes.
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Bundalovic-Torma, Cedoljub, Lonjon, Fabien, Desveaux, Darrell, and Guttman, David S.
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Pseudomonas syringae is an evolutionarily diverse bacterial species complex and a preeminent model for the study of plant–pathogen interactions due in part to its remarkably broad host range. A critical feature of P. syringae virulence is the employment of suites of type III secreted effector (T3SE) proteins, which vary widely in composition and function. These effectors act on a variety of plant intracellular targets to promote pathogenesis but can also be avirulence factors when detected by host immune complexes. In this review, we survey the phylogenetic diversity (PD) of the P. syringae effectorome, comprising 70 distinct T3SE families identified to date, and highlight how avoidance of host immune detection has shaped effectorome diversity through functional redundancy, diversification, and horizontal transfer. We present emerging avenues for research and novel insights that can be gained via future investigations of plant–pathogen interactions through the fusion of large-scale interaction screens and phylogenomic approaches. [ABSTRACT FROM AUTHOR]
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- 2022
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21. The type III effector HopF[2.sub.pto] targets Arabidopsis RIN4 protein to promote Pseudomonas syringae virulence
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Wilton, Mike, Subramaniam, Rajagopal, Elmore, James, Felsensteiner, Corinna, Coaker, Gitta, and Desveaux, Darrell
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Virulence (Microbiology) -- Research ,Plant immunology -- Research ,Arabidopsis thaliana -- Physiological aspects ,Arabidopsis thaliana -- Research ,Science and technology - Abstract
Plant immunity can be induced by two major classes of pathogen-associated molecules. Pathogen- or microbe-associated molecular patterns (PAMPs or MAMPs) are conserved molecular components of microbes that serve as 'non-self' features to induce PAMP-triggered immunity (PTI). Pathogen effector proteins used to promote virulence can also be recognized as 'non-self' features or induce a 'modified-self' state that can induce effector-triggered immunity (ETI). The Arabidopsis protein RIN4 plays an important role in both branches of plant immunity. Three unrelated type III secretion effector (TESE) proteins from the phytopathogen Pseudomonas syringae, AvrRpml, AvrRpt2, and AvrB, target RIN4, resulting in ETI that effectively restricts pathogen growth. However, no pathogenic advantage has been demonstrated for RIN4 manipulation by these TTSEs. Here, we show that the TTSE HopF[2.sub.Pto] also targets Arabidopsis RIN4. Transgenic plants conditionally expressing HopF[2.sub.Pto] were compromised for AvrRpt2-induced RIN4 modification and associated ETI. HopF[2.sub.Pto] interfered with AvrRpt2-induced RIN4 modification in vitro but not with AvrRpt2 activation, suggestive of RIN4 targeting by HopF[2.sub.Pto]. In support of this hypothesis, HopF[2.sub.Pto] interacted with RIN4 in vitro and in vivo. Unlike AvrRpm1, AvrRpt2, and AvrB, HopF[2.sub.Pto] did not induce ETI and instead promoted P. syringae growth in Arabidopsis. This virulence activity was not observed in plants genetically lacking RIN4. These data provide evidence that RIN4 is a major virulence target of HopF[2.sub.Pto] and that a pathogenic advantage can be conveyed by TTSEs that target RIN4. bacterial virulence | type III secretion doi/10.1073/pnas.0904739107
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- 2010
22. Predictive modeling of Pseudomonas syringae virulence on bean using gradient boosted decision trees.
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Almeida, Renan N. D., Greenberg, Michael, Bundalovic-Torma, Cedoljub, Martel, Alexandre, Wang, Pauline W., Middleton, Maggie A., Chatterton, Syama, Desveaux, Darrell, and Guttman, David S.
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PSEUDOMONAS syringae ,DECISION trees ,PREDICTION models ,BEANS ,COMMON bean ,DIAGNOSTIC microbiology - Abstract
Pseudomonas syringae is a genetically diverse bacterial species complex responsible for numerous agronomically important crop diseases. Individual P. syringae isolates are assigned pathovar designations based on their host of isolation and the associated disease symptoms, and these pathovar designations are often assumed to reflect host specificity although this assumption has rarely been rigorously tested. Here we developed a rapid seed infection assay to measure the virulence of 121 diverse P. syringae isolates on common bean (Phaseolus vulgaris). This collection includes P. syringae phylogroup 2 (PG2) bean isolates (pathovar syringae) that cause bacterial spot disease and P. syringae phylogroup 3 (PG3) bean isolates (pathovar phaseolicola) that cause the more serious halo blight disease. We found that bean isolates in general were significantly more virulent on bean than non-bean isolates and observed no significant virulence difference between the PG2 and PG3 bean isolates. However, when we compared virulence within PGs we found that PG3 bean isolates were significantly more virulent than PG3 non-bean isolates, while there was no significant difference in virulence between PG2 bean and non-bean isolates. These results indicate that PG3 strains have a higher level of host specificity than PG2 strains. We then used gradient boosting machine learning to predict each strain's virulence on bean based whole genome k-mers, type III secreted effector k-mers, and the presence/absence of type III effectors and phytotoxins. Our model performed best using whole genome data and was able to predict virulence with high accuracy (mean absolute error = 0.05). Finally, we functionally validated the model by predicting virulence for 16 strains and found that 15 (94%) had virulence levels within the bounds of estimated predictions. This study strengthens the hypothesis that P. syringae PG2 strains have evolved a different lifestyle than other P. syringae strains as reflected in their lower level of host specificity. It also acts as a proof-of-principle to demonstrate the power of machine learning for predicting host specific adaptation. Author summary: Pseudomonas syringae is a genetically diverse Gammaproteobacterial species complex responsible for numerous agronomically important crop diseases. Strains in the P. syringae species complex are frequently categorized into pathovars depending on pathogenic characteristics such as host of isolation and disease symptoms. Common bean pathogens from P. syringae are known to cause two major diseases: (1) pathovar phaseolicola strains from phylogroup 3 cause halo blight disease, characterized by large necrotic lesions surrounded by a chlorotic zone or halo of yellow tissue; and (2) pathovar syringae strains from phylogroup 2 causes bacterial spot disease, characterized by brown leaf spots. While halo blight can cause serious crop losses, bacterial spot disease is generally of minor agronomic concern. Recently, statistical genetic and machine learning approaches have been applied to genomic data to identify genes underlying traits of interest or predict the outcome of host-microbe interactions. Here, we apply machine learning to P. syringae genomic data to predict virulence on bean. We first characterized the virulence of P. syringae isolates on common bean using a seed infection assay and then applied machine learning to the genomic data from the same strains to generate a predictive model for virulence on bean. We found that machine learning models built with k-mers from either full genome data or virulence factors could predict bean virulence with high accuracy. We also confirmed prior work showing that phylogroup 3 halo blight pathogens display a stronger degree of phylogenetic clustering and host specificity compared to phylogroup 2 brown spot pathogens. This works serves as a proof-of-principle for the power of machine learning for predicting host specificity and may find utility in agricultural diagnostic microbiology. [ABSTRACT FROM AUTHOR]
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- 2022
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23. Metaeffector interactions modulate the type III effector-triggered immunity load of Pseudomonas syringae.
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Martel, Alexandre, Laflamme, Bradley, Breit-McNally, Clare, Wang, Pauline, Lonjon, Fabien, Desveaux, Darrell, and Guttman, David S.
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PSEUDOMONAS syringae ,PHYTOPATHOGENIC microorganisms ,HOST plants ,DISEASE resistance of plants ,ARABIDOPSIS thaliana ,IMMUNITY - Abstract
The bacterial plant pathogen Pseudomonas syringae requires type III secreted effectors (T3SEs) for pathogenesis. However, a major facet of plant immunity entails the recognition of a subset of P. syringae's T3SEs by intracellular host receptors in a process called Effector-Triggered Immunity (ETI). Prior work has shown that ETI-eliciting T3SEs are pervasive in the P. syringae species complex raising the question of how P. syringae mitigates its ETI load to become a successful pathogen. While pathogens can evade ETI by T3SE mutation, recombination, or loss, there is increasing evidence that effector-effector (a.k.a., metaeffector) interactions can suppress ETI. To study the ETI-suppression potential of P. syringae T3SE repertoires, we compared the ETI-elicitation profiles of two genetically divergent strains: P. syringae pv. tomato DC3000 (PtoDC3000) and P. syringae pv. maculicola ES4326 (PmaES4326), which are both virulent on Arabidopsis thaliana but harbour largely distinct effector repertoires. Of the 529 T3SE alleles screened on A. thaliana Col-0 from the P. syringae T3SE compendium (PsyTEC), 69 alleles from 21 T3SE families elicited ETI in at least one of the two strain backgrounds, while 50 elicited ETI in both backgrounds, resulting in 19 differential ETI responses including two novel ETI-eliciting families: AvrPto1 and HopT1. Although most of these differences were quantitative, three ETI responses were completely absent in one of the pathogenic backgrounds. We performed ETI suppression screens to test if metaeffector interactions contributed to these ETI differences, and found that HopQ1a suppressed AvrPto1m-mediated ETI, while HopG1c and HopF1g suppressed HopT1b-mediated ETI. Overall, these results show that P. syringae strains leverage metaeffector interactions and ETI suppression to overcome the ETI load associated with their native T3SE repertoires. Author summary: Pathogens such as Pseudomonas syringae use a diverse array of virulence proteins, termed effectors, to mediate disease progression. However, plant hosts have evolved the capacity to recognize a subset of these effector proteins to mount a robust effector-triggered immune (ETI) response. We previously found that ETI elicitation is a prominent feature of P. syringae effector repertoires, leading to the hypothesis that P. syringae strains must possess ETI mitigation strategies to be pathogenic. In this study we screened a comprehensive compendium of P. syringae effectors in the strain PmaES4326 and found two ETI eliciting effector families that were not identified using the strain PtoDC3000, which possesses a distinct effector repertoire. We demonstrate that these differences are due to ETI suppression by PtoDC3000 effectors, highlighting how pathogenic effector repertoires can create networks that mitigate the ETI load of a given pathogenic strain. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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24. The HopZ family of Pseudomonas syringae type III effectors require myristoylation for virulence and avirulence functions in arabidopsis thaliana
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Lewis, Jennifer D., Abada, Wasan, Ma, Wenbo, Guttman, David S., and Desveaux, Darrell
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Plant proteins -- Properties ,Arabidopsis thaliana -- Genetic aspects ,Pseudomonas syringae -- Genetic aspects ,Virulence (Microbiology) -- Evaluation ,Translocation (Genetics) -- Identification and classification ,Biological sciences - Abstract
Pseudomonas syringae utilizes the type III secretion system to translocate effector proteins into plant cells, where they can contribute to the pathogen's ability to infect and cause disease. Recognition of these effectors by resistance proteins induces defense responses that typically include a programmed cell death reaction called the hypersensitive response. The YopJ/HopZ family of type III effector proteins is a common family of effector proteins found in animal- and plant-pathogenic bacteria. The HopZ family in P. syringae includes HopZla.sub.PsyA2], Hop[Zlb.sub.PgyUnB647], Hop[Z1c.sub.PmaE54326], Hop[Z2.sub.Ppi895A] and Hop[Z3.sub.PsyB728a.] HopZla is predicted to be most similar to the ancestral hopZ allele and causes a hypersensitive response in multiple plant species, including Arabidopsis thaliana. Therefore, it has been proposed that host defense responses have driven the diversification of this effector family. In this study, we further characterized the hypersensitive response induced by HopZla and demonstrated that it is not dependent on known resistance genes. Further, we identified a novel virulence function for HopZ2 that requires the catalytic cysteine demonstrated to be required for protease activity. Sequence analysis of the HopZ family revealed the presence of a predicted myristoylation sequence in all members except HopZ3. We demonstrated that the myristoylation site is required for membrane localization of this effector family and contributes to the virulence and avirulence activities of HopZ2 and HopZla, respectively. This paper provides insight into the selective pressures driving virulence protein evolution by describing a detailed functional characterization of the diverse HopZ family of type III effectors with the model plant Arabidopsis.
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- 2008
25. Direct visualization of protein interactions in plant cells
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Subramaniam, Rajagopal, Desveaux, Darrell, Spickler, Catherine, Michnick, Stephen W., and Brisson, Normand
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- 2001
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26. RecPD: A Recombination-aware measure of phylogenetic diversity.
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Bundalovic-Torma, Cedoljub, Desveaux, Darrell, and Guttman, David S.
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PHYTOPATHOGENIC bacteria , *HORIZONTAL gene transfer , *SPECIES diversity , *PSEUDOMONAS syringae , *MICROBIAL genomes , *POPULATION genetics , *GENE families , *GENETIC variation - Abstract
A critical step in studying biological features (e.g., genetic variants, gene families, metabolic capabilities, or taxa) is assessing their diversity and distribution among a sample of individuals. Accurate assessments of these patterns are essential for linking features to traits or outcomes of interest and understanding their functional impact. Consequently, it is of crucial importance that the measures employed for quantifying feature diversity can perform robustly under any evolutionary scenario. However, the standard measures used for quantifying and comparing the distribution of features, such as prevalence, phylogenetic diversity, and related approaches, either do not take into consideration evolutionary history, or assume strictly vertical patterns of inheritance. Consequently, these approaches cannot accurately assess diversity for features that have undergone recombination or horizontal transfer. To address this issue, we have devised RecPD, a novel recombination-aware phylogenetic-diversity statistic for measuring the distribution and diversity of features under all evolutionary scenarios. RecPD utilizes ancestral-state reconstruction to map the presence / absence of features onto ancestral nodes in a species tree, and then identifies potential recombination events in the evolutionary history of the feature. We also derive several related measures from RecPD that can be used to assess and quantify evolutionary dynamics and correlation of feature evolutionary histories. We used simulation studies to show that RecPD reliably reconstructs feature evolutionary histories under diverse recombination and loss scenarios. We then applied RecPD in two diverse real-world scenarios including a preliminary study type III effector protein families secreted by the plant pathogenic bacterium Pseudomonas syringae and growth phenotypes of the Pseudomonas genus and demonstrate that prevalence is an inadequate measure that obscures the potential impact of recombination. We believe RecPD will have broad utility for revealing and quantifying complex evolutionary processes for features at any biological level. Author summary: Phylogenetic diversity is an important concept utilized in evolutionary ecology which has extensive applications in population genetics to help us understand how evolutionary processes have distributed genetic variation among individuals of a species, and how this impacts phenotypic diversification over time. However, existing approaches for studying phylogenetic diversity largely assume that the genetic features follow vertical inheritance, which is frequently violated in the case of microbial genomes due to horizontal transfer. To address this shortcoming, we present RecPD, a recombination-aware phylogenetic diversity measure, which incorporates ancestral state reconstruction to quantify the phylogenetic diversity of genetic features mapped onto a species phylogeny. Through simulation experiments we show that RecPD robustly reconstructs the evolutionary histories of features evolving under various scenarios of recombination and loss. When applied to a real-world example of type III secreted effector protein families from the plant pathogenic bacterium Pseudomonas syringae, RecPD reveals that horizontal transfer has played an important role in shaping the phylogenetic distributions of a substantial proportion of families across the P. syringae species complex. Furthermore, we demonstrate that the traditional measures of feature prevalence are unsuitable as a measure for comparing feature diversity. We also provide a R package implementation of RecPD for public use: https://github.com/cedatorma/recpd. [ABSTRACT FROM AUTHOR]
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- 2022
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27. AlgW regulates multiple Pseudomonas syringae virulence strategies
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Schreiber, Karl J. and Desveaux, Darrell
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- 2011
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28. The small molecule Zaractin activates ZAR1-mediated immunity in Arabidopsis.
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Derek Seto, Khan, Madiha, Bastedo, D. Patrick, Martel, Alexandre, Trinh Vo, Guttman, David, Subramaniam, Rajagopal, and Desveaux, Darrell
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SMALL molecules ,ARABIDOPSIS ,PSEUDOMONAS syringae ,IMMUNITY ,IMMUNE system - Abstract
Pathogenic effector proteins use a variety of enzymatic activities to manipulate host cellular proteins and favor the infection process. However, these perturbations can be sensed by nucleotide-binding leucine-rich-repeat (NLR) proteins to activate effector-triggered immunity (ETI). Here we have identified a small molecule (Zaractin) that mimics the immune eliciting activity of the Pseudomonas syringae type III secreted effector (T3SE) HopF1r and show that both HopF1r and Zaractin activate the same NLR-mediated immune pathway in Arabidopsis. Our results demonstrate that the ETIinducing action of pathogenic effectors can be harnessed to identify synthetic activators of the eukaryotic immune system. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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29. A high-throughput chemical screen for resistance to Pseudomonas syringae in Arabidopsis
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Schreiber, Karl, Ckurshumova, Wenzislava, Peek, James, and Desveaux, Darrell
- Published
- 2008
30. Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole
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Schreiber Karl J, Austin Ryan S, Gong Yunchen, Zhang Jianfeng, Fung Pauline, Wang Pauline W, Guttman David S, and Desveaux Darrell
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Chemical genomics ,Sulfanilamides ,Arabidopsis thaliana ,Botany ,QK1-989 - Abstract
Background The sulfanilamide family comprises a clinically important group of antimicrobial compounds which also display bioactivity in plants. While there is evidence that sulfanilamides inhibit folate biosynthesis in both bacteria and plants, the complete network of plant responses to these compounds remains to be characterized. As such, we initiated two forward genetic screens in Arabidopsis in order to identify mutants that exhibit altered sensitivity to sulfanilamide compounds. These screens were based on the growth phenotype of seedlings germinated in the presence of the compound sulfamethoxazole (Smex). Results We identified a mutant with reduced sensitivity to Smex, and subsequent mapping indicated that a gene encoding 5-oxoprolinase was responsible for this phenotype. A mutation causing enhanced sensitivity to Smex was mapped to a gene lacking any functional annotation. Conclusions The genes identified through our forward genetic screens represent novel mediators of Arabidopsis responses to sulfanilamides and suggest that these responses extend beyond the perturbation of folate biosynthesis.
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- 2012
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31. Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2
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Lewis Jennifer D, Wan Janet, Ford Rachel, Gong Yunchen, Fung Pauline, Nahal Hardeep, Wang Pauline W, Desveaux Darrell, and Guttman David S
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Next-generation sequencing ,yeast two-hybrid ,high-throughput screening ,Arabidopsis ,Pseudomonas syringae ,type III effector ,MLO2 ,HopZ ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Identification of protein-protein interactions is a fundamental aspect of understanding protein function. A commonly used method for identifying protein interactions is the yeast two-hybrid system. Results Here we describe the application of next-generation sequencing to yeast two-hybrid interaction screens and develop Quantitative Interactor Screen Sequencing (QIS-Seq). QIS-Seq provides a quantitative measurement of enrichment for each interactor relative to its frequency in the library as well as its general stickiness (non-specific binding). The QIS-Seq approach is scalable and can be used with any yeast two-hybrid screen and with any next-generation sequencing platform. The quantitative nature of QIS-Seq data make it amenable to statistical evaluation, and importantly, facilitates the standardization of experimental design, data collection, and data analysis. We applied QIS-Seq to identify the Arabidopsis thaliana MLO2 protein as a target of the Pseudomonas syringae type III secreted effector protein HopZ2. We validate the interaction between HopZ2 and MLO2 in planta and show that the interaction is required for HopZ2-associated virulence. Conclusions We demonstrate that QIS-Seq is a high-throughput quantitative interactor screen and validate MLO2 as an interactor and novel virulence target of the P. syringae type III secreted effector HopZ2.
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- 2012
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32. Immunodiversity of the Arabidopsis ZAR1 NLR Is Conveyed by Receptor-Like Cytoplasmic Kinase Sensors.
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Martel, Alexandre, Laflamme, Bradley, Seto, Derek, Bastedo, D. Patrick, Dillon, Marcus M., Almeida, Renan N. D., Guttman, David S., and Desveaux, Darrell
- Subjects
ARABIDOPSIS ,PSEUDOMONAS syringae ,DETECTORS ,IMMUNE response - Abstract
The Arabidopsis nucleotide-binding leucine-rich repeat protein ZAR1 can recognize at least six distinct families of pathogenic effector proteins to mount an effector-triggered immune response. This remarkable immunodiversity appears to be conveyed by receptor-like cytoplasmic kinase (RLCK) complexes, which associate with ZAR1 to sense several effector-induced kinase perturbations. Here we show that the recently identified ZAR1-mediated immune responses against the HopX1, HopO1, and HopBA1 effector families of Pseudomonas syringae rely on an expanded diversity of RLCK sensors. We show that individual sensors can recognize distinct effector families, thereby contributing to the expanded surveillance potential of ZAR1 and supporting its role as a guardian of the plant kinome. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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33. The pan-genome effector-triggered immunity landscape of a host-pathogen interaction.
- Author
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Laflamme, Bradley, Dillon, Marcus M., Martel, Alexandre, Almeida, Renan N. D., Desveaux, Darrell, and Guttman, David S.
- Published
- 2020
- Full Text
- View/download PDF
34. A host–pathogen interactome uncovers phytopathogenic strategies to manipulate plant ABA responses.
- Author
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Cao, Feng Y., Khan, Madiha, Taniguchi, Masatoshi, Mirmiran, Armand, Moeder, Wolfgang, Lumba, Shelley, Yoshioka, Keiko, and Desveaux, Darrell
- Subjects
ARABIDOPSIS proteins ,ABSCISIC acid ,PSEUDOMONAS syringae ,DISEASE resistance of plants ,PHYTOPATHOGENIC microorganisms ,HOST plants - Abstract
Summary: The phytopathogen Pseudomonas syringae delivers into host cells type III secreted effectors (T3SEs) that promote virulence. One virulence mechanism employed by T3SEs is to target hormone signaling pathways to perturb hormone homeostasis. The phytohormone abscisic acid (ABA) influences interactions between various phytopathogens and their plant hosts, and has been shown to be a target of P. syringae T3SEs. In order to provide insight into how T3SEs manipulate ABA responses, we generated an ABA‐T3SEinteractome network (ATIN) between P. syringae T3SEs and Arabidopsis proteins encoded by ABA‐regulated genes. ATIN consists of 476 yeast‐two‐hybrid interactions between 97 Arabidopsis ABA‐regulated proteins and 56 T3SEs from four pathovars of P. syringae. We demonstrate that T3SE interacting proteins are significantly enriched for proteins associated with transcription. In particular, the ETHYLENE RESPONSIVE FACTOR (ERF) family of transcription factors is highly represented. We show that ERF105 and ERF8 displayed a role in defense against P. syringae, supporting our overall observation that T3SEs of ATIN converge on proteins that influence plant immunity. In addition, we demonstrate that T3SEs that interact with a large number of ABA‐regulated proteins can influence ABA responses. One of these T3SEs, HopF3Pph6, inhibits the function of ERF8, which influences both ABA‐responses and plant immunity. These results provide a potential mechanism for how HopF3Pph6 manipulates ABA‐responses to promote P. syringae virulence, and also demonstrate the utility of ATIN as a resource to study the ABA‐T3SE interface. Significance Statement: Phytohormones, such as abscisic acid (ABA), are manipulated by plant pathogens to promote pathogenesis, yet the molecular details of this host sabotage remain largely enigmatic. We present an interactome between the pathogenic effectors of Pseudomonas syringae and Arabidopsis thaliana ABA‐regulated proteins as a resource to probe this phytohormone–pathogen interface. We demonstrate that this interactome can be used to identify effectors that manipulate ABA signaling, as well as components of plant immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
35. Perturbations of the ZED1 pseudokinase activate plant immunity.
- Author
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Bastedo, D. Patrick, Khan, Madiha, Martel, Alexandre, Seto, Derek, Kireeva, Inga, Zhang, Jianfeng, Masud, Wardah, Millar, David, Lee, Jee Yeon, Lee, Amy Huei-Yi, Gong, Yunchen, Santos-Severino, André, Guttman, David S., and Desveaux, Darrell
- Subjects
SECRETION ,DISEASE resistance of plants ,RECEPTOR-like kinases ,HOST plants ,TERNARY forms ,PSEUDOMONAS syringae - Abstract
The Pseudomonas syringae acetyltransferase HopZ1a is delivered into host cells by the type III secretion system to promote bacterial growth. However, in the model plant host Arabidopsis thaliana, HopZ1a activity results in an effector-triggered immune response (ETI) that limits bacterial proliferation. HopZ1a-triggered immunity requires the nucleotide-binding, leucine-rich repeat domain (NLR) protein, ZAR1, and the pseudokinase, ZED1. Here we demonstrate that HopZ1a can acetylate members of a family of ‘receptor-like cytoplasmic kinases’ (RLCK family VII; also known as PBS1-like kinases, or PBLs) and promote their interaction with ZED1 and ZAR1 to form a ZAR1-ZED1-PBL ternary complex. Interactions between ZED1 and PBL kinases are determined by the pseudokinase features of ZED1, and mutants designed to restore ZED1 kinase motifs can (1) bind to PBLs, (2) recruit ZAR1, and (3) trigger ZAR1-dependent immunity in planta, all independently of HopZ1a. A ZED1 mutant that mimics acetylation by HopZ1a also triggers immunity in planta, providing evidence that effector-induced perturbations of ZED1 also activate ZAR1. Overall, our results suggest that interactions between these two RLCK families are promoted by perturbations of structural features that distinguish active from inactive kinase domain conformations. We propose that effector-induced interactions between ZED1/ZRK pseudokinases (RLCK family XII) and PBL kinases (RLCK family VII) provide a sensitive mechanism for detecting perturbations of either kinase family to activate ZAR1-mediated ETI. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
36. Molecular Evolution of Pseudomonas syringae Type III Secreted Effector Proteins.
- Author
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Dillon, Marcus M., Almeida, Renan N.D., Laflamme, Bradley, Martel, Alexandre, Weir, Bevan S., Desveaux, Darrell, and Guttman, David S.
- Subjects
PSEUDOMONAS syringae ,HORIZONTAL gene transfer ,HOST plants ,MOLECULAR evolution ,DISEASE resistance of plants ,GLOBAL analysis (Mathematics) - Abstract
Diverse Gram-negative pathogens like Pseudomonas syringae employ type III secreted effector (T3SE) proteins as primary virulence factors that combat host immunity and promote disease. T3SEs can also be recognized by plant hosts and activate an effector triggered immune (ETI) response that shifts the interaction back toward plant immunity. Consequently, T3SEs are pivotal in determining the virulence potential of individual P. syringae strains, and ultimately help to restrict P. syringae pathogens to a subset of potential hosts that are unable to recognize their repertoires of T3SEs. While a number of effector families are known to be present in the P. syringae species complex, one of the most persistent challenges has been documenting the complex variation in T3SE contents across a diverse collection of strains. Using the entire pan-genome of 494 P. syringae strains isolated from more than 100 hosts, we conducted a global analysis of all known and putative T3SEs. We identified a total of 14,613 putative T3SEs, 4,636 of which were unique at the amino acid level, and show that T3SE repertoires of different P. syringae strains vary dramatically, even among strains isolated from the same hosts. We also find substantial diversification within many T3SE families, and in many cases find strong signatures of positive selection. Furthermore, we identify multiple gene gain and loss events for several families, demonstrating an important role of horizontal gene transfer (HGT) in the evolution of P. syringae T3SEs. These analyses provide insight into the evolutionary history of P. syringae T3SEs as they co-evolve with the host immune system, and dramatically expand the database of P. syringae T3SEs alleles. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
37. Building a protein-interaction network to study <italic>Fusarium graminearum</italic> pathogenesis.
- Author
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Mirmiran, Armand, Desveaux, Darrell, and Subramaniam, Rajagopal
- Subjects
- *
FUSARIUM , *FUSARIUM diseases of plants , *FUSARIUM toxins , *DEOXYNIVALENOL , *BIOSYNTHESIS - Abstract
Protein interaction networks provide invaluable information on the complexity of biological pathways within organisms. They not only allow for key proteins to be identified within the network but also allow us to identify groups of closely associated proteins with common biological functions. These properties allow for a better understanding of the protein organization in a cell, and the specific interactions within individual biological processes provide a tool to predict functions of unknown proteins. Given the significant amount of time and cost associated with producing protein interaction networks, the majority of them have been generated for model organisms. For other organisms, protein interactions are predicted based on previously existing networks using Interolog and large-scale gene expression databases. Although these predicted networks are valuable, they can only provide information on proteins shared across species, such as those responsible for primary metabolism. As such, networks of unique secondary metabolism pathways, such as the biosynthesis of deoxynivalenol (DON) in
Fusarium graminearum , cannot be extrapolated using existing resources in other organisms. Therefore, we propose a sFu arium N etwork ofT richotheceneA ssociatedP roteins (FuNTAP) based on yeast two-hybrid interactions of proteins that are differentially expressed under DON inducing conditions. FuNTAP should allow a characterization of the metabolic pathway by which this important metabolite is synthesized without recourse to predictions based on earlier model interactomes. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
38. Oh, the places they'll go! A survey of phytopathogen effectors and their host targets.
- Author
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Khan, Madiha, Seto, Derek, Subramaniam, Rajagopal, and Desveaux, Darrell
- Subjects
PHYTOPATHOGENIC microorganisms ,PLANT viruses ,MICROBIAL virulence ,OOMYCETES ,PSEUDOMONAS syringae ,RALSTONIA ,XANTHOMONAS - Abstract
Summary: Phytopathogens translocate effector proteins into plant cells where they sabotage the host cellular machinery to promote infection. An individual pathogen can translocate numerous distinct effectors during the infection process to target an array of host macromolecules (proteins, metabolites, DNA, etc.) and manipulate them using a variety of enzymatic activities. In this review, we have surveyed the literature for effector targets and curated them to convey the range of functions carried out by phytopathogenic proteins inside host cells. In particular, we have curated the locations of effector targets, as well as their biological and molecular functions and compared these properties across diverse phytopathogens. This analysis validates previous observations about effector functions (e.g. immunosuppression), and also highlights some interesting features regarding effector specificity as well as functional diversification of phytopathogen virulence strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
39. The study of pattern-triggered immunity in Arabidopsis.
- Author
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Mott, G. Adam, Guttman, David S., and Desveaux, Darrell
- Subjects
ARABIDOPSIS ,PATTERN perception receptors ,DISEASE resistance of plants ,PLANT defenses ,CROP improvement - Abstract
Copyright of Canadian Journal of Plant Pathology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
- Full Text
- View/download PDF
40. The HopF family of Pseudomonas syringae type III secreted effectors.
- Author
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Lo, Timothy, Koulena, Noushin, Seto, Derek, Guttman, David S., and Desveaux, Darrell
- Subjects
PSEUDOMONAS syringae ,PLANT viruses ,HOST plants ,AGRONOMY ,AGRICULTURAL diversification - Abstract
Pseudomonas syringae is a bacterial phytopathogen that utilizes the type III secretion system to inject effector proteins into plant host cells. Pseudomonas syringae can infect a wide range of plant hosts, including agronomically important crops such as tomatoes and beans. The ability of P. syringae to infect such numerous hosts is caused, in part, by the diversity of effectors employed by this phytopathogen. Over 60 different effector families exist in P. syringae; one such family is HopF, which contains over 100 distinct alleles. Despite this diversity, research has focused on only two members of this family: HopF1 from P. syringae pathovar phaseolicola 1449B and HopF2 from P. syringae pathovar tomato DC3000. In this study, we review the research on HopF family members, including their host targets and molecular mechanisms of immunity suppression, and their enzymatic function. We also provide a phylogenetic analysis of this expanding effector family which provides a basis for a proposed nomenclature to guide future research. The extensive genetic diversity that exists within the HopF family presents a great opportunity to study how functional diversification on an effector family contributes to host specialization. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
41. New BAR tools for mining expression data and exploring Cis-elements in Arabidopsis thaliana.
- Author
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Austin, Ryan S., Hiu, Shu, Waese, Jamie, Ierullo, Matthew, Pasha, Asher, Wang, Ting Ting, Fan, Jim, Foong, Curtis, Breit, Robert, Desveaux, Darrell, Moses, Alan, and Provart, Nicholas J.
- Subjects
GENE expression in plants ,ARABIDOPSIS thaliana ,PLANT genetics ,GENE regulatory networks ,GENETIC markers in plants - Abstract
Identifying sets of genes that are specifically expressed in certain tissues or in response to an environmental stimulus is useful for designing reporter constructs, generating gene expression markers, or for understanding gene regulatory networks. We have developed an easy-to-use online tool for defining a desired expression profile (a modification of our Expression Angler program), which can then be used to identify genes exhibiting patterns of expression that match this profile as closely as possible. Further, we have developed another online tool, Cistome, for predicting or exploring cis-elements in the promoters of sets of co-expressed genes identified by such a method, or by other methods. We present two use cases for these tools, which are freely available on the Bio-Analytic Resource at . [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
42. The ERF11 Transcription Factor Promotes Internode Elongation by Activating Gibberellin Biosynthesis and Signaling.
- Author
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Xin Zhou, Zhong-Lin Zhang, Jeongmoo Park, Ludmila Tyler, Jikumaru Yusuke, Kai Qiu, Nam, Edward A., Lumba, Shelley, Desveaux, Darrell, McCourt, Peter, Yuji Kamiya, and Tai-ping Sun
- Published
- 2016
- Full Text
- View/download PDF
43. Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation.
- Author
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Mott, G. Adam, Thakur, Shalabh, Smakowska, Elwira, Wang, Pauline W., Belkhadir, Youssef, Desveaux, Darrell, and Guttman, David S.
- Published
- 2016
- Full Text
- View/download PDF
44. Elevated Temperature Differentially Influences Effector-Triggered Immunity Outputs in Arabidopsis.
- Author
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Menna, Alexandra, Nguyen, Dang, Guttman, David S., and Desveaux, Darrell
- Subjects
ARABIDOPSIS thaliana ,PSEUDOMONAS syringae ,EFFECT of temperature on plants - Abstract
Pseudomonas syringae is a Gram-negative bacterium that infects multiple plant species by manipulating cellular processes via injection of type three secreted effectors (T3SEs) into host cells. Nucleotide-binding leucine-rich repeat (NLR) resistance (R) proteins recognize specific T3SEs and trigger a robust immune response, called effector-triggered immunity (ETI), which limits pathogen proliferation and is often associated with localized programmed cell death, known as the hypersensitive response (HR). In this study, we examine the influence of elevated temperature on two ETI outputs: HR and pathogen virulence suppression. We found that in the Arabidopsis thaliana accession Col-0, elevated temperatures suppress the HR, but have minimal influence on ETI-associated P. syringae virulence suppression, thereby uncoupling these two ETI responses. We also identify accessions of Arabidopsis that exhibit impaired P. syringae virulence suppression at elevated temperature, highlighting the natural variation that exists in coping with biotic and abiotic stresses. These results not only reinforce the influence of abiotic factors on plant immunity but also emphasize the importance of carefully documented environmental conditions in studies of plant immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
45. Immunomodulation by the Pseudomonas syringae HopZ Type III Effector Family in Arabidopsis.
- Author
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Lewis, Jennifer D., Wilton, Mike, Mott, G . Adam, Lu, Wenwan, Hassan, Jana A., Guttman, David S., and Desveaux, Darrell
- Subjects
BACTERIAL diseases ,IMMUNOREGULATION ,PSEUDOMONAS syringae ,ARABIDOPSIS ,HOST plants ,PLANT immunology - Abstract
Pseudomonas syringae employs a type III secretion system to inject 20–30 different type III effector (T3SE) proteins into plant host cells. A major role of T3SEs is to suppress plant immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are a superfamily of T3SEs found in both plant and animal pathogenic bacteria. In P. syringae, this superfamily includes the evolutionarily diverse HopZ1, HopZ2 and HopZ3 alleles. To investigate the roles of the HopZ family in immunomodulation, we generated dexamethasone-inducible T3SE transgenic lines of Arabidopsis for HopZ family members and characterized them for immune suppression phenotypes. We show that all of the HopZ family members can actively suppress various facets of Arabidopsis immunity in a catalytic residue-dependent manner. HopZ family members can differentially suppress the activation of mitogen-activated protein (MAP) kinase cascades or the production of reactive oxygen species, whereas all members can promote the growth of non-virulent P. syringae. Localization studies show that four of the HopZ family members containing predicted myristoylation sites are localized to the vicinity of the plasma membrane while HopZ3 which lacks the myristoylation site is at least partially nuclear localized, suggesting diversification of immunosuppressive mechanisms. Overall, we demonstrate that despite significant evolutionary diversification, all HopZ family members can suppress immunity in Arabidopsis. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
46. The Pseudomonas syringae Type III Effector HopF2 Suppresses Arabidopsis Stomatal Immunity.
- Author
-
Hurley, Brenden, Lee, Donghyuk, Mott, Adam, Wilton, Michael, Liu, Jun, Liu, Yulu C., Angers, Stephane, Coaker, Gitta, Guttman, David S., and Desveaux, Darrell
- Subjects
PSEUDOMONAS syringae ,ARABIDOPSIS ,PLANT immunology ,PLANT cellular signal transduction ,PLANT enzymes ,ENZYME activation - Abstract
Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases required for regulating stomatal aperture, suggesting HopF2 may manipulate stomatal immunity. Here we report HopF2 can inhibit stomatal immunity independent of its ADP-ribosyltransferase activity. Transgenic expression of HopF2 in Arabidopsis inhibits stomatal closing in response to P. syringae and increases the virulence of surface inoculated P. syringae. Further, transgenic expression of HopF2 inhibits flg22 induced reactive oxygen species production. Intriguingly, ADP-ribosyltransferase activity is dispensable for inhibiting stomatal immunity and flg22 induced reactive oxygen species. Together, this implies HopF2 may be a bifunctional T3SE with ADP-ribosyltransferase activity required for inhibiting apoplastic immunity and an independent function required to inhibit stomatal immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
47. Peptides and small molecules of the plant-pathogen apoplastic arena.
- Author
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Mott, G. Adam, Middleton, Maggie A., Desveaux, Darrell, and Guttman, David S.
- Subjects
PHYTOPATHOGENIC microorganisms ,PLANT growth ,PEPTIDES ,MOLECULES ,LEAVES ,PLANT defenses - Abstract
Plants reside within an environment rich in potential pathogens. Survival in the presence of such threats requires both effective perception of, and appropriate responses to, pathogenic attack. While plants lack an adaptive immune system, they have a highly developed and responsive innate immune system able to detect and inhibit the growth of the vast majority of potential pathogens. Many of the critical interactions that characterize the relationship between plants and pathogens are played out in the intercellular apoplastic space. The initial perception of pathogen invasion is often achieved through specific plant receptor-like kinases that recognize conserved molecular patterns presented by the pathogen or respond to the molecular debris caused by cellular damage. The perception of either microbial or damage signals by these receptors initiates a response that includes the production of peptides and small molecules to enhance cellular integrity and inhibit pathogen growth. In this review, we discuss the roles of apoplastic peptides and small molecules in modulating plant-pathogen interactions. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
48. Proteomics of effector-triggered immunity (ETI) in plants.
- Author
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Hurley, Brenden, Subramaniam, Rajagopal, Guttman, David S, and Desveaux, Darrell
- Abstract
Effector-triggered immunity (ETI) was originally termed gene-for-gene resistance and dates back to fundamental observations of flax resistance to rust fungi by Harold Henry Flor in the 1940s. Since then, genetic and biochemical approaches have defined our current understanding of how plant “resistance” proteins recognize microbial effectors. More recently, proteomic approaches have expanded our view of the protein landscape during ETI and contributed significant advances to our mechanistic understanding of ETI signaling. Here we provide an overview of proteomic techniques that have been used to study plant ETI including both global and targeted approaches. We discuss the challenges associated with ETI proteomics and highlight specific examples from the literature, which demonstrate how proteomics is advancing the ETI research field. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
49. The ABCs and 123s of Bacterial Secretion Systems in Plant Pathogenesis.
- Author
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Chang, Jeff H., Desveaux, Darrell, and Creason, Allison L.
- Subjects
- *
BACTERIAL secretions , *PHYTOPATHOGENIC bacteria , *PLANT cells & tissues , *PLANT proteins , *PLANT diseases - Abstract
Bacteria have many export and secretion systems that translocate cargo into and across biological membranes. Seven secretion systems contribute to pathogenicity by translocating proteinaceous cargos that can be released into the extracellular milieu or directly into recipient cells. In this review, we describe these secretion systems and how their complexities and functions reflect differences in the destinations, states, functions, and sizes of the translocated cargos as well as the architecture of the bacterial cell envelope. We examine the secretion systems from the perspective of pathogenic bacteria that proliferate within plant tissues and highlight examples of translocated proteins that contribute to the infection and disease of plant hosts. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
50. Arabidopsis GOLDEN2-LIKE ( GLK) transcription factors activate jasmonic acid ( JA)-dependent disease susceptibility to the biotrophic pathogen Hyaloperonospora arabidopsidis, as well as JA-independent plant immunity against the necrotrophic pathogen Botrytis cinerea
- Author
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Murmu, Jhadeswar, Wilton, Michael, Allard, Ghislaine, Pandeya, Radhey, Desveaux, Darrell, Singh, Jas, and Subramaniam, Rajagopal
- Subjects
ARABIDOPSIS ,TRANSCRIPTION factors ,JASMONIC acid ,DISEASE susceptibility ,DISEASE resistance of plants ,PHYTOPATHOGENIC microorganisms ,CHLOROPLASTS - Abstract
Arabidopsis thaliana GOLDEN2- LIKE ( GLK1 and 2) transcription factors regulate chloroplast development in a redundant manner. Overexpression of AtGLK1 (35S: AtGLK1) in Arabidopsis also confers resistance to the cereal pathogen Fusarium graminearum. To further elucidate the role of GLK transcription factors in plant defence, the Arabidopsis glk1 glk2 double-mutant and 35S: AtGLK1 plants were challenged with the virulent oomycete pathogen Hyaloperonospora arabidopsidis ( Hpa) Noco2. Compared with Col-0, glk1 glk2 plants were highly resistant to Hpa Noco2, whereas 35S: AtGLK1 plants showed enhanced susceptibility to this pathogen. Genetic studies suggested that AtGLK-mediated plant defence to Hpa Noco2 was partially dependent on salicylic acid ( SA) accumulation, but independent of the SA signalling protein NONEXPRESSOR OF PATHOGENESIS- RELATED 1 ( NPR1). Pretreatment with jasmonic acid ( JA) dramatically reversed Hpa Noco2 resistance in the glk1 glk2 double mutant, but only marginally affected the 35S: AtGLK1 plants. In addition, overexpression of AtGLK1 in the JA signalling mutant coi1-16 did not increase susceptibility to Hpa Noco2. Together, our GLK gain-of-function and loss-of-function experiments suggest that GLK acts upstream of JA signalling in disease susceptibility to Hpa Noco2. In contrast, glk1 glk2 plants were more susceptible to the necrotrophic fungal pathogen Botrytis cinerea, whereas 35S: AtGLK1 plants exhibited heightened resistance which could be maintained in the absence of JA signalling. Together, the data reveal that AtGLK1 is involved in JA-dependent susceptibility to the biotrophic pathogen Hpa Noco2 and in JA-independent resistance to the necrotrophic pathogen B. cinerea. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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