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Abstract Adhesion G Protein Coupled Receptors (aGPCRs) have evolved an activation mechanism to translate extracellular force into liberation of a tethered agonist (TA) to effect cell signalling. We report here that ADGRF1 can signal through all major G protein classes and identify the structural basis for a previously reported Gαq preference by cryo-EM. Our structure shows that Gαq preference in ADGRF1 may derive from tighter packing at the conserved F569 of the TA, altering contacts between TM helix I and VII, with a concurrent rearrangement of TM helix VII and helix VIII at the site of Gα recruitment. Mutational studies of the interface and of contact residues within the 7TM domain identify residues critical for signalling, and suggest that Gαs signalling is more sensitive to mutation of TA or binding site residues than Gαq. Our work advances the detailed molecular understanding of aGPCR TA activation, identifying features that potentially explain preferential signal modulation.

Voltage-gated ion channels (VGICs) respond to changes in membrane potential (Vm) and typically exhibit fast kinetic properties. They play an important role in signal detection and propagation in excitable tissues. In contrast, the role of VGICs in non-excitable tissues like epithelia is less studied and less clear. Studies in epithelia of vertebrates and invertebrates demonstrate wide expression of VGICs in epithelia of animals. Recently, VGICs have emerged as regulators of ion transport in the Malpighian tubules (MTs) and other osmoregulatory organs of insects. This mini-review aims to concisely summarize which VGICs have been implicated in the regulation of ion transport in the osmoregulatory epithelia of insects to date, and highlight select groups for further study. We have also speculated on the roles VGICs may potentially play in regulating processes connected directly to ion transport in insects (e.g., acid-base balance, desiccation, thermal tolerance). This review is not meant to be exhaustive but should rather serve as a thought-provoking collection of select existing highlights on VGICs, and to emphasize how understudied this mechanism of ion transport regulation is in insect epithelia. [ABSTRACT FROM AUTHOR]

Nowadays there is a large number of cars in the world, they have overcrowded the cities, therefore the parking places are becoming more and more difficult to find. This reason has led in finding a solution for increasing the number of car parks on a small area. The paper proposes the development, functional description and testing of a smart parking system demonstrator based on elevator principal. The demonstrator can be controlled with the help of an android application which is connected to an Arduino via bluetooth.

Optimity Advisors conducted the study “Health system performance assessment – Integrated Care Assessment”, commissioned by Chafea and DG SANTE, assessing: (i) the state of integrated care (IC) implementation in the EU28 Member States, Norway and Iceland; and (ii) developing an evidence-based Integrated Care Performance Assessment (ICPA) framework. The framework, supported by an Excel tool, was co-designed with stakeholders across Europe. The ICPA tool will be tested by members of the European Innovation Partnership on Active and Healthy Ageing (EIPonAHA), B3 Action Group on Integrated Care. From a review of literature in 24 languages, an extensive mapping of 560 IC initiatives in 30 countries and the maturity assessment of 12 health systems, we inferred that IC strategies, policies, models and projects are present across Europe, but their characteristics, depth and breadth of integration vary considerably. Taking this heterogeneity into account, the framework was co-designed drawing on user-centred design and agile methodologies. Following an iterative approach with regular stakeholder engagement (questionnaires, video-conferences, peer-review webinars, presentations at events, and a validation workshop), experts and practitioners helped us determine which indicators sourced from existing performance assessment frameworks were considered “core” to IC assessment, with the rest proposed as “optional” indicators in the performance assessment model. The core indicators selected were categorised under four domains -Advancement of integration; Use of care services; Health outcomes; Experiences of care- with a fifth overarching domain considering financial issues. Indicators have been re-worded and re-defined to make them applicable across the wealth of IC initiatives while offering measures for international comparison. The heterogeneity of the context in which IC initiatives operate in Europe was a key challenge for the development of a framework that had to be relevant and potentially used in all included countries. This was addressed by providing an additional Excel-based ICPA tool offering flexibility in the use of the framework and allowing users to adapt indicators to their context. The tool allows users to compare performance to a set target for each indicator in the ICPA framework. The use of the ICPA framework and tool will help healthcare systems across Europe to further develop their IC system - based on their current state and context - and could significantly help them achieve better health outcomes and care experiences. The use of user-centred design and agile methodologies are proved methods of finding useful solutions to common challenges. Our research indicates that these principles also apply in the area of health performance assessment and can support IC implementation. Without user engagement, there was a risk that the output of the study would not provide the value sought. As IC evolves, the framework will need to be updated and adapted to new circumstances. Further user engagement and testing is recommended to ensure usability. With the research concluded, the framework and tool will need to be tested in practice through the involvement of members of the B3 Action Group of the EIPonAHA. Preliminary results will be resented at ICIC19. A web-based tool could facilitate uptake and data collection and analysis.

All EU28 countries, Norway and Iceland, run Integrated Care Initiatives (ICI) in efforts to address the new demographic challenges, reduce costs and improve health outcomes. As part of a study commissioned by Chafea and DG SANTE, Optimity Advisors has conducted an extensive review and mapping of ICI across Europe, to assess the level of penetration and adoption of ICI in the 30 countries and evaluate the level of maturity of several healthcare systems. This has allowed to examine the extent of the use of digital tools in ICI. The aim of this presentation will be to look at the relationship between theory and practice, assessing whether assessments of a country’s healthcare system in terms of its Digital Health maturity is reflected in its number of digitally-enabled ICI. For each country, the Study Team, in collaboration with researchers from all included nationalities, has identified 573 ICI and analysed their characteristics and implementation. Considering the different elements of the selected initiatives, it was possible to assess the extent to which Digital Health was included in each country. Additional desk research and the use of the Scirocco Maturity Assessment Tool (which has “information and e-Health” as one of its 12 assessment criteria) have then allowed to examine whether a correlation between the number of digitally-enabled ICI per country and the maturity of their integrated care systems exists. Our research has shown that all EU28 countries, Norway and Iceland, have implemented e-Health Strategies. The review found that IC strategies, policies and projects are present in all 30 countries, although their characteristics, depth and breadth of integration are varied. However, based on our repository of ICI, only 79, out of the 573 ICI retrieved, included a digital component. This translates to less than 15%, spread across 18 countries (Spain (16); UK (11); Lithuania (9); Italy (6); Germany (6); Czech Republic (5); Belgium (5); Greece (4); Finland (4); Slovakia (3); Hungary (2); France (2); Cyprus (1); Iceland (1); Norway (1); Croatia (1); Denmark (1); Austria (1)). Contrary to our assumptions, the assessment of integrated care system maturity did not always reflect the number of ICI with digital components retrieved in the repository. For example, while stakeholders from Denmark and Iceland, have self-assessed their “information and e-Health” domain as highly advanced, both countries only have one digitally-enabled initiative listed in the repository. Moreover, while the evidence around integrated care shows that Digital Health is a key component of an integrated system, in practice less than 15% of all the retrieved ICI had a digital component in it. There is therefore a lack of clarity on whether ICI working on health and social care programmes and strategies see e-Health and m-Health as central elements of the integration, or if Digital Health is rather perceived as an additional and complementary element that would assist, rather than drive, their projects. Additional research focusing on the role of Digital Health in practice and on how to raise the Digital Health profile in the ICI landscape across Europe should be considered.

Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function.

Notch signaling relies on ligand-induced proteolysis of the transmembrane receptor Notch to liberate a nuclear effector that drives cell fate decisions. Upon ligand binding, sequential cleavage of Notch by the transmembrane protease ADAM10 and the intracellular protease γ-secretase releases the Notch intracellular domain (NICD), which translocates to the nucleus and forms a complex that induces target gene transcription. To map the location and timing of the individual steps required for the proteolysis and movement of Notch from the plasma membrane to the nucleus, we used proximity labeling with quantitative, multiplexed mass spectrometry to monitor the interaction partners of endogenous NOTCH2 after ligand stimulation in the presence of a γ-secretase inhibitor and as a function of time after inhibitor removal. Our studies showed that γ-secretase–mediated cleavage of NOTCH2 occurred in an intracellular compartment and that formation of nuclear complexes and recruitment of chromatin-modifying enzymes occurred within 45 min of inhibitor washout. These findings provide a detailed spatiotemporal map tracking the path of Notch from the plasma membrane to the nucleus and identify signaling events that are potential targets for modulating Notch activity. Editor's summary: Ligand binding to the transmembrane receptor Notch induces two proteolytic cleavage events that liberate its intracellular domain (NICD), which translocates to the nucleus to modulate gene expression. Martin et al. generated a time-resolved map of the intracellular proteins encountered by endogenous NOTCH2/NICD2 as it transited to the nucleus in stimulated human cells. The second cleavage event triggering NICD2 release occurred at an intracellular compartment, and NICD2 then transiently interacted with proteins that link membranes to the actin cytoskeleton. Such time-resolved proximity mapping may be used to track the paths of other signaling proteins in cells. —Annalisa M. VanHook [ABSTRACT FROM AUTHOR]

This study examined the association between ownership of high-risk ("vicious") dogs and the presence of deviant behaviors in the owners as indicated by court convictions. We also explored whether two characteristics of dog ownership (abiding licensing laws and choice of breed) could be useful areas of inquiry when assessing risk status in settings where children are present. Our matched sample consisted of 355 owners of either licensed or cited dogs that represented high or low-risk breeds. Categories of criminal convictions examined were aggressive crimes, drugs, alcohol, domestic violence, crimes involving children, firearm convictions, and major and minor traffic citations. Owners of cited high-risk ("vicious") dogs had significantly more criminal convictions than owners of licensed low-risk dogs. Findings suggest that the ownership of a high-risk ("vicious") dog can be a significant marker for general deviance and should be an element considered when assessing risk for child endangerment. (Contains 3 tables and 4 figures.)

Presents nine responses of professors and communication professionals to the ethical, economic, and enduring issues of journalism and mass communication education. Considers their responses to the state of journalism and mass communication education and reevaluates educational assumptions and practices. (SG)

Media's perception of race and its portrayal of minority communities are perverted because of the predominance of whites' attitudes in the mainstream media. The imagery of blacks is not based on reality but on popular misconceptions. The attitude of media authorities has also influenced the young people of minority communities who do not have faith in themselves to become a part of the American culture.

Reveals that identification as a liberal publication was a significant factor in media treatment of Jackson's candidacy in only one of nine comparisons, while identification as a conservative publication was a factor in only three of nine. The most important independent factor was the identification of the publication as a newspaper. (FL)

Students from eight Baltimore City Public Schools were given the Multifactor Racial Attitude Inventory (MRAI) to survey attitudes towards Blacks in television programs. Results indicated that, although Blacks identified strongly and more positively with Black television characters, there was no consistent pattern relative to racial attitudes and viewer perception. (MER)

The portrayal of women of color on TV has typically originated with white men, and limited opportunities remain for black women in TV shows. A stereotypical role has been as a domestic worker. This role can be broadened, as on 'Maude' and 'Good Times.' The maid in 'I'll Fly Away' keeps a journal, which indicates her intelligence. Roles for black women have expanded to include situation comedies, but parts in TV movies remain scarce.

The article focuses on the need to enforce firm laws to include non-white and female faculty in colleges in the fields of journalism and mass communications.

African American people in US feel that it is important that there be more of their people writing, producing and directing. There is discrimination of African Americans in the entertainment industry as they are considered good for comedies but issues related to the realities of their life's ups and downs are not often shown on weekly network television.

Nowadays there is a large number of cars in the world, they have overcrowded the cities, therefore the parking places are becoming more and more difficult to find. This reason has led in finding a solution for increasing the number of car parks on a small area. The paper proposes the development, functional description and testing of a smart parking system demonstrator based on elevator principal. The demonstrator can be controlled with the help of an android application which is connected to an Arduino via bluetooth. [ABSTRACT FROM AUTHOR]

Whenever journalism educators discuss accreditation, standards 12 and 3 are the ones that elicit the lion's share of attention. Standard 12, Diversity, focuses on a school's commitment to increased diversity [...]

Prime-time television shows have not been effective in building a positive image of Blacks in the US despite the large number of black performers, directors and managers. Only a handful of shows have exerted an effort to present an image of Blacks different from the singers, dancers and servant roles that are most often given to Blacks. Notable among these shows are 'Frank's Place' and 'Equal Justice' for their realistic depiction of Blacks from the Black perspective. Television programmers should reorient their policies given the growing importance of Blacks as consumers.

Copyright of Family Process is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The draft nuclear genome sequence of the snail-transmitted, dimorphic, parasitic, platyhelminth Schistosoma mansoni revealed eight genes encoding proteins that contain the Universal Stress Protein (USP) domain. Schistosoma mansoni is a causative agent of human schistosomiasis, a severe and debilitating Neglected Tropical Disease (NTD) of poverty, which is endemic in at least 76 countries. The availability of the genome sequences of Schistosoma species presents opportunities for bioinformatics and genomics analyses of associated gene families that could be targets for understanding schistosomiasis ecology, intervention, prevention and control. Proteins with the USP domain are known to provide bacteria, archaea, fungi, protists and plants with the ability to respond to diverse environmental stresses. In this research investigation, the functional annotations of the USP genes and predicted nucleotide and protein sequences were initially verified. Subsequently, sequence clusters and distinctive features of the sequences were determined. A total of twelve ligand binding sites were predicted based on alignment to the ATP-binding universal stress protein from Methanocaldococcus jannaschii. In addition, six USP sequences showed the presence of ATP-binding motif residues indicating that they may be regulated by ATP. Public domain gene expression data and RT-PCR assays confirmed that all the S. mansoni USP genes were transcribed in at least one of the developmental life cycle stages of the helminth. Six of these genes were up-regulated in the miracidium, a free-swimming stage that is critical for transmission to the snail intermediate host. It is possible that during the intra-snail stages, S. mansoni gene transcripts for universal stress proteins are low abundant and are induced to perform specialized functions triggered by environmental stressors such as oxidative stress due to hydrogen peroxide that is present in the snail hemocytes. This report serves to catalyze the formation of a network of researchers to understand the function and regulation of the universal stress proteins encoded in genomes of schistosomes and their snail intermediate hosts. [ABSTRACT FROM AUTHOR]

An introduction to the journal is presented in which the author discusses the initial findings from local site evaluations of Grants for the Benefit of Homeless Individuals (GBHI), presents an evaluation of evidence-based practices tailored to homeless subpopulations with co-occurring disorders and discusses an article on implementing Assertive Community Treatment (ACT) with adults who are homeless and have co-occurring mental and addictive disorders.

Fidelity rating of evidence-based practices ensures the implementation of interventions as intended and enhances the likelihood of replication of positive outcomes previously demonstrated. However, modifications have been necessary when a practice is adopted in settings or for populations other than those for which they were developed. This study reports fidelity findings for six programs implementing the Assertive Community Treatment model in different settings for adults who are homeless and who present with mental and addictive disorders, together with a methodology that can assist programs to achieve fidelity. Initial and final fidelity ratings using the Dartmouth Assertive Community Treatment Scale were collected from six programs. Findings indicate that while fidelity in programs can vary over time, core elements can be maintained, resulting in at least moderate fidelity in each program and modest overall increases across all programs combined with concomitant decreases in the variance of fidelity scales. The findings support previous research that fidelity change is minimal after initial fidelity is achieved during the first year of implementation, and they extend previous results to programs serving persons who are homeless and have co-occurring mental and addictive disorders. This study suggests that fidelity to evidence-based practice can be maintained for adaptation to new populations and settings. Understanding areas that are likely to be challenging to fidelity achievement and managing the variability of performance on the components of each fidelity aspect can assist the program manager and personnel to achieve and maintain adequate model fidelity. [ABSTRACT FROM AUTHOR]

The Assertive Community Treatment model (ACT) was developed more than 30 years ago to treat individuals with serious and persistent mental illness. This qualitative study highlights practical challenges encountered when establishing ACT teams in diverse settings serving people who are homeless with co-occurring mental and addictive disorders (COD). Program administrators and evaluators from nine programs located in seven states completed a survey on implementation challenges, fidelity, modifications to the ACT model, and program successes. Challenges encountered related largely to staffing and funding limitations as well as to difficulties with implementing the ACT model without modifications. Several modifications to the model were believed beneficial to recruiting and retaining consumers. These included emphasizing housing, adding staff positions not prescribed by ACT, implementing mini-teams within the program, delivering in-office services in a group format, and placing time-limited services by transitioning consumers to less intensive settings. Successes included reduction in hospitalizations, psychiatric symptoms, and substance abuse. Stabilization of consumers was attributed largely to housing assistance and maintenance; medication adherence; and delivery of intensive, multidisciplinary services including substance abuse treatment. Implications of this study suggest the need to adapt the ACT model for people who are homeless with COD by tailoring program staffing and service delivery. Furthermore, there is a need for a measure capable of assessing ACT fidelity in the context of both housing models and integrated treatment for the homeless population. [ABSTRACT FROM AUTHOR]

Despite the unique and challenging circumstances confronting Latino immigrant families, debate still exists as to the need to culturally adapt evidence-based interventions for dissemination with this population. Following the grounded theory approach, the current qualitative investigation utilized focus group interviews with 83 Latino immigrant parents to explore the relevance of culturally adapting an evidence-based parenting intervention to be disseminated within this population. Findings from this study indicate that Latino immigrant parents want to participate in a culturally adapted parenting intervention as long as it is culturally relevant, respectful, and responsive to their life experiences. Research results also suggest that the parenting skills participants seek to enhance are among those commonly targeted by evidence-based parenting interventions. This study contributes to the cultural adaptation/fidelity balance debate by highlighting the necessity of exploring ways to develop culturally adapted interventions characterized by high cultural relevance, as well as high fidelity to the core components that have established efficacy for evidence-based parenting interventions. [ABSTRACT FROM AUTHOR]

This study provides a coding framework to audit empirically a museum's image in the media. The final aim is to help museums consider the media as active participants and co-constructors of their image and, therefore, as key actors building credibility, institutional power, prestige, familiarity and trust toward the organization. The application of the audit, in a museum which was in the media's spotlight for scandals, shows that the proposed methodology provides two main insights. First, it provides a picture of how media portray the museum in its functional and emotional/representational image, with the advantage of knowing how it is covered in its uniqueness and inimitability. Secondly, it gives details on which features of the museum's image receive positive, negative or no coverage at all, with the benefit of recognizing which critical issues and opportunities should be managed to improve the museum's image in the media. [ABSTRACT FROM AUTHOR]

This study explored the effectiveness of the Accrediting Council on Education in Journalism and Mass Communications' diversity standard from 1989 to 2002. Although this standard, known as Standard 12, had the most non-compliances, the findings credit the standard for increasing non-white and female faculty and students in accredited journalism units. The findings also credit the standard for the development of courses that focus on issues pertaining to women and non-whites in journalism. But the study also concluded that stronger enforcement and additional strategies were needed to fully achieve diversity in journalism and mass communication education. [ABSTRACT FROM AUTHOR]

The article presents a debate related to the question "Does journalism education matter? Jannette L. Dates argues that a prepare journalism curricula must be done to promote and upgrade the standards of journalism, the enterprise essential to democracy. Theodore L. Glasser argues in his essay on why journalism matters, that journalism studies will only make a difference if they makes journalism better and help them understand what journalism means. Mitchell Stephens introduces 13 principles that he contends that is needed for practical journalism education. G. Stuart Adam argues for an increased connection between the applied field of journalism and the intellectual culture of the university.

The author analyzes the influences of black women as decision makers and creators in entertainment television and focuses on the effects black women have had on the system during the period 1999-2004. She also assesses whether a black presence has made a difference in the process and the products produced for television viewers. [ABSTRACT FROM AUTHOR]