Search

Despite recent efforts to search for biomarkers for the pre-symptomatic diagnosis of Parkinson's disease (PD), the presence of risk factors, prodromal signs, and family history still support the classification of individuals at risk for this disease. Human epidemiological studies are useful in this search but fail to provide causality. The study of well-known risk factors for PD in animal models can help elucidate mechanisms related to the disease's etiology and contribute to future prevention or treatment approaches. This narrative review aims to discuss animal studies that investigated four of the main risk factors and/or prodromal signs related to PD: advanced age, male sex, sleep alterations, and depression. Different databases were used to search the studies, which were included based on their relevance to the topic. Although still in a reduced number, such studies are of great relevance in the search for evidence that leads to a possible early diagnosis and improvements in methods of prevention and treatment. [ABSTRACT FROM AUTHOR]

Copyright of HOLOS is the property of Instituto Federal do Rio Grande do norte - IFRN and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Based on data collected from managers of wine tourism businesses in rural areas in Portugal, this study highlights the equal potential of female leadership in wine tourism. Specifically, women’s profiles, management practices and leadership narratives were analyzed and contrasted with corresponding data reported by their male counterparts. Concerning personality traits, no statistical difference between men and women was identified, which was also the case for some management practices, with sustainability concerns being important for both male and female business managers. Differences were found in communication with markets, being dominated by online devices, particularly with social networks in the case of women. As for their leadership position, women further tend to prefer a shared management approach in opposition to a more individualist management style followed by men.

Wine tourism (WT) is an important area of special-interest tourism in Portugal, and represents an increasingly significant component of regional development. In a more conservative approach, WT has been described as visiting vineyards, wineries, and engaging in wine-related activities. However, this perspective has been broadened, taking advantage of all the potential of the specific destination’s terroir (nature/landscape, tangible and intangible cultural heritage, etc.). Wine routes make the connection between wine and tourism in a specific region and intend to boost wine tourism by promoting collaboration between different stakeholders. Different routes present distinct approaches to WT, within diverse regional contexts, and different ways of collaboration within the wine route. This study compares three wine routes at different stages of development, located in a rural periphery – in the central region of Portugal – Bairrada, Dão, and Beira Interior, considering both context data and information collected in 113 interviews conducted with diverse wine tourism agents from these routes. Besides a brief characterization of the three routes, the main results indicate supplier’s preference for terroir routes instead of wine routes and show the importance of gender, age, and education level for the collaborative work between stakeholders. These aspects and their contribution to the development of WT routes are discussed. Some questions that additional studies may help answering are also reflected.

Optical Tamm states (OTS) are confined optical modes that can occur at the interface between two highly reflective structures. However, due to the strong reflectance required, their implementation with highly processable and metal-free flexible materials has proven challenging. Herein, we develop the first structure supporting OTS based only on organic polymeric materials, demonstrating a photonic platform based on non-critical, widely available and easily processable materials. The structures fabricated present large areas and consist of a narrowband multi-layered polymeric distributed Bragg reflector (DBR) followed by a thin film of J-aggregate molecular excitonic material that can act as a highly reflective surface within a narrowband range. We take advantage of the narrowband spectral response of the DBR and of the reflective molecular layer to tune the OTS band by varying the periodicity of the multilayer, opening the door for the fabrication of OTS structures based on lightweight integrable excitonic devices with cost-effective procedures.

The objective of this study was to assess the influence of chlorhexidine (liquid and gel) and zinc oxide in calcium hydroxide (CH) pastes on root pH in simulated external resorption. One hundred human anterior teeth with a single root canal were selected. After decoronation and root canal instrumentation, the specimens were divided into 4 experimental groups and 1 control group (without intracanal paste): CH + saline (CH+S), CH + 2% chlorhexidine liquid (CH+ CHX), CH + 2% chlorhexidine gel (CH+ CHXg), and CH + 2% chlorhexidine gel + zinc oxide (CH+ CHXg+ZnO). pH was measured using a microelectrode at 3 and 24 h, and 1, 2, 3, and 4 weeks after inserting intracanal pastes. Data were analyzed statistically using an ANOVA and Tukey's test (p < 0.05). The CH+CHXg+ZnO group had the highest pH values throughout (p<0.05). The CH+S and CH+ CHX groups had the highest pH values after 1 week and the CH+ CHXg group after 2 weeks. CH+ CHXg maintained the highest pH until the fourth week compared with CH+ CHX (p < 0.05). The control group remained at a neutral pH at all evaluated times. It can be concluded that chlorhexidine solution or gel maintained the alkaline pH of CH, and chlorhexidine gel allowed a slower decrease in pH over time. CH+ CHXg+ZnO showed the highest pH values and was an effective intracanal medication for maintaining alkaline root pH in the area of resorption. [ABSTRACT FROM AUTHOR]

Hepatitis E has always been related to morbidity in pregnant women. Its epidemiology is not well understood in Brazil. Therefore, we tested sera from 209 pregnant women and 199 female blood donors, collected at a single center in Curitiba, Brazil. The Wantai assay was used for testing the anti‐hepatitis E virus (anti‐HEV), immunoglobulin G (IgG), and an in‐house polymerase chain reaction process for testing HEV RNA. Anti‐HEV was detected in 22.5% of the total group, 19% in the pregnant women group, and 26% in the blood donor group (P = 0.11), a much higher prevalence when compared with other studies in Brazil. Demographical analysis showed that 92.4% were born in the South Region of Brazil, 4.9% in the Southeast, and 2.7% were distributed over other regions of the country. With respect to their origin, 99% were from the South, 0.7% from the Southeast, and 0.2% from the Central‐West regions. Income, education, race, number of pregnancies, and abortion did differ significantly when comparing both the groups (P < 0.001). Age >30 (P = 0.012) and the number (>3) of pregnancies (P = 0.008) were related to anti‐HEV positivity. All anti‐HEV IgG–positive females were HEV RNA negative. In conclusion, HEV positivity was found in one out of five young women, which showed an urgent need for further epidemiological studies in Brazil. [ABSTRACT FROM AUTHOR]

When a dysfunction occurs in any component of the stomatognathic system, temporomandibular disorders ( TMD) may originate. The aim of this study was to compare the deviations, displacement and the execution speed of mandibular movements among asymptomatic participants and those with TMD. Convenience sampling was used; forty participants diagnosed by clinical evaluation following the Research Diagnostic Criteria for Temporomandibular Disorders were divided into three groups: arthropathy ( GART, 10 participants, 40% men), myopathy ( GMYO, 10 participants, 30% men), and the control group ( CG, 20 asymptomatic participants, 25% men). Participants were asked to perform the movements of free maximal mouth opening and closing, right and left lateral excursions, and protrusion with sliding teeth contacts. The mandibular trajectory was recorded using opto-electronic devices tracking reflective markers placed in front of the 'soft tissue pogonion point'. The movements were analysed on the following axis: x - medial-lateral, y - vertical, z - antero-posterior. Significative differences were found in CGx GART - unassisted maximal mouth opening and closing projection on y-axis (OCY), CGx GMYO - unassisted maximal mouth opening and closing projection on x-axis (OCX), and in the measures Opening lateral deviation on x-axis (OLDX), closing lateral deviation on x-axis (CLDX) and in the measures of speed for both. In regard to GARTx GMYO, a significative difference was found in Protrusion lateral deviation on x-axis (PLDX) 'Conover-Iman Test of Multiple Comparisons Using Rank Sums' using Bonferroni correction ( P < 0·05). In conclusion, the total opening movements in individuals with TMD tended to have higher deviation than in those asymptomatic individuals and a reduction in the speed of movements. [ABSTRACT FROM AUTHOR]

Shiga toxin-producing Escherichia coli (STEC) is a known food pathogen, which main reservoir is the intestine of ruminants. The abundance of different STEC lineages in nature reflect a heterogeneity that is characterised by the differential expression of certain genotypic characteristics, which in turn are influenced by the environmental conditions to which the microorganism is exposed. Bacterial homeostasis and stress response are under the control of the alarmone guanosine tetraphosphate (ppGpp), which intrinsic levels varies across the E. coli species. In the present study, 50 STEC isolates from healthy sheep were evaluated regarding their ppGpp content, cytotoxicity and other relevant genetic and phenotypic characteristics. We found that the level of ppGpp and cytotoxicity varied considerably among the examined strains. Isolates that harboured the stx2 gene were the least cytotoxic and presented the highest levels of ppGpp. All stx2 isolates belonged to phylogroup A, while strains that carried stx1 or both stx1 and stx2 genes pertained to phylogroup B1. All but two stx2 isolates belonged to the stx2b subtype. Strains that belonged to phylogroup B1 displayed on average low levels of ppGpp and high cytotoxicity. Overall, there was a negative correlation between cytotoxicity and ppGpp. [ABSTRACT FROM AUTHOR]

Copyright of Archivos de Zootecnia is the property of Archivos de Zootecnia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Brazilian Journal of Biology is the property of Instituto Internacional de Ecologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Scientific investigations in the mineral processing area by flotation have demonstrated that both coarse particles (Dp > 300 ?m) and fine particles (Dp < 10 ?m) show a small flotation efficiency. An effective way to improve the flotation of fine particles is decrease the bubble size, promoting an increase of both holdup and bubble surface area flux, thus, increasing the probability of bubbleparticle collision. However, an excessive decrease of the bubble size may cause an overloading of the bubbles and, therefore, a decrease in flotation efficiency. Thus, the aim of this work was to evaluate the flotation of quartz particles with different bubble sizes (0.50 mm < Db mean < 2.9 mm), using an automatized mini pilot flotation column. The results obtained showed that the flotation efficiencies of coarse particles (D32 = 345 ?m) and fine particles (D32 = 25 ?m) were improved with a decrease in bubble size. [ABSTRACT FROM AUTHOR]

Health-care workers (HCWs) on the frontlines of the 2019 Coronavirus Disease 2019 (COVID-19) have a high risk of acquiring and dying from the disease. Brazil is a country with high morbimortality and ranks highest in general mortality from COVID-19. A large proportion of medical doctors (MDs) is dying, impacting in the workforce. As part of the research by Fiocruz on working conditions among HCWs, we have conducted this study of death among MDs. We analyzed data from Federal Council of Medicine, the only national platform with details about deaths in MDs. A broad search of other sources, including the Ministry of Health, was done, but unfortunately, the data does not exist. A total of 622 MDs died from March 2020 to March 2021: 87.6% male and 75% over age 60. The Southeast had the highest percentage (34.7%), followed by the Northeast (27.2%), and rankings by state showed Rio de Janeiro (15.8%), followed by São Paulo, Pará, Paraná and Paraíba, totalizing 50.1%. The specialties with highest mortality were gynecology (12.2%), pediatrics (10.0%), internal medicine (9.3%), general surgery (7.8%) and cardiology (7.6%). May 2020 was the month of worst mortality (16.9%) followed by July and June. A slow decline was observed until October, when Brazil begun to experience the ‘‘second wave’’. The vaccination of MDs began at the end of January 2021 and has not been sufficient time to evaluate the impact on morbimortality. The results of a study of 50 professional categories are under analysis and will be published soon as well as a study of ‘‘invisible’’ HCWs, who do not have specific training but are essential to supporting health care system, from primary care units to high-complexity hospitals. We hope that the results of these studies will improve links among managers, those who define public policies, and union leaders, achieving better workplace and living conditions, minimizing health and socioeconomic disparities. Key messages: This study is about death by COVID-19 among medical doctors in Brazil, as part of a large one on professional categories, essentials to supporting health care system, conducted by FIOCRUZ. This stdy is part of a large one on professional categories, essentials to supporting health care system, conducted by FIOCRUZ. [ABSTRACT FROM AUTHOR]

Context. The detection of reflected light from an exoplanet is a difficult technical challenge at optical wavelengths. Even though this signal is expected to replicate the stellar signal, not only is it several orders of magnitude fainter, but it is also hidden among the stellar noise. Aims. We apply a variant of the cross-correlation technique to HARPS observations of 51 Peg to detect the reflected signal from planet 51 Peg b. Methods. Our method makes use of the cross-correlation function (CCF) of a binary mask with high-resolution spectra to amplify the minute planetary signal that is present in the spectra by a factor proportional to the number of spectral lines when performing the cross correlation. The resulting cross-correlation functions are then normalized by a stellar template to remove the stellar signal. Carefully selected sections of the resulting normalized CCFs are stacked to increase the planetary signal further. The recovered signal allows probing several of the planetary properties, including its real mass and albedo. Results. We detect evidence for the reflected signal from planet 51 Peg b at a significance of 3σnoise. The detection of the signal permits us to infer a real mass of 0:46+0:06 >-0:01 MJup (assuming a stellar mass of 1:04 MSun) for the planet and an orbital inclination of 80+10-19 degrees. The analysis of the data also allows us to infer a tentative value for the (radius-dependent) geometric albedo of the planet. The results suggest that 51Peg b may be an inflated hot Jupiter with a high albedo (e.g., an albedo of 0.5 yields a radius of 1:9 ± 0:3 RJup for a signal amplitude of 6:0 ± 0:4 × 10-5). Conclusions. We confirm that the method we perfected can be used to retrieve an exoplanet's reflected signal, even with current observing facilities. The advent of next generation of instruments (e.g. VLT-ESO/ESPRESSO) and observing facilities (e.g. a new generation of ELT telescopes) will yield new opportunities for this type of technique to probe deeper into exoplanets and their atmospheres. [ABSTRACT FROM AUTHOR]

Context. In the near future, new instruments such as ESPRESSO will arrive, allowing us to reach a precision in radial velocity measurements on the order of 10 cm s–1. At this level of precision, several noise sources that until now have been outweighed by photon noise will start to contribute significantly to the error budget. The telluric lines that are not neglected by the masks for the radial velocity computation, here called micro-telluric lines, are one such noise source. Aims. In this work we investigate the impact of micro-telluric lines in the radial velocities calculations. We also investigate how to correct the effect of these atmospheric lines on radial velocities. Methods. The work presented here follows two parallel lines. First, we calculated the impact of the micro-telluric lines by multiplying a synthetic solar-like stellar spectrum by synthetic atmospheric spectra and evaluated the effect created by the presence of the telluric lines. Then, we divided HARPS spectra by synthetic atmospheric spectra to correct for its presence on real data and calculated the radial velocity on the corrected spectra. When doing so, one considers two atmospheric models for the synthetic atmospheric spectra: the LBLRTMand TAPAS. Results. We find that the micro-telluric lines can induce an impact on the radial velocity calculation that can already be close to the current precision achieved with HARPS, and so its effect should not be neglected, especially for future instruments such as ESPRESSO.Moreover, we find that the micro-telluric lines' impact depends on factors, such as the radial velocity of the star, airmass, relative humidity, and the barycentric Earth radial velocity projected along the line of sight at the time of the observation. [ABSTRACT FROM AUTHOR]

Diets rich in saturated fats may contribute to the loss of pancreatic β-cells in type 2 diabetes. JunB, a member of the activating protein 1 (AP-1) transcription factor family, promotes β-cell survival and mediates part of the beneficial effects of GLP-1 agonists. In this study we interrogated the molecular mechanisms involved in JunB-mediated β-cell protection from lipotoxicity. The saturated fatty acid palmitate decreased JunB expression, and this loss may contribute to β-cell apoptosis, as overexpression of JunB protected cells from lipotoxicity. Array analysis of JunB-deficient β-cells identified a gene expression signature of a downregulated endoplasmic reticulum (ER) stress response and inhibited AKT signaling. JunB stimulates XBP1 expression via the transcription factor c/EBPδ during ER stress, and forced expression of XBP1s rescued the viability of JunB-deficient cells, constituting an important antiapoptotic mechanism. JunB silencing inhibited AKT activation and activated the proapoptotic Bcl-2 protein BAD via its dephosphorylation. BAD knockdown reversed lipotoxic β-cell death potentiated by JunB siRNA. Interestingly, XBP1s links JunB and AKT signaling as XBP1 knockdown also reduced AKT phosphorylation. GLP-1 agonists induced cAMP-dependent AKT phosphorylation leading to β-cell protection against palmitate-induced apoptosis. JunB and XBP1 knockdown or IRE1 inhibition decreased AKT activation by cAMP, leading to β-cell apoptosis. In conclusion, JunB modulates the β-cell ER stress response and AKT signaling via the induction of XBP1s. The activation of the JunB gene network and the crosstalk between the ER stress and AKT pathway constitute a crucial defense mechanism by which GLP-1 agonists protect against lipotoxic β-cell death. These findings elucidate novel β-cell-protective signal transduction in type 2 diabetes. [ABSTRACT FROM AUTHOR]

Context. The search for planets orbiting metal-poor stars is of utmost importance for our understanding of planet formation models. However, no dedicated searches have been conducted so far for very low mass planets orbiting such objects. Only a few cases of low-mass planets orbiting metal-poor stars are thus known. Amongst these, HD?41248 is a metal-poor, solar-type star on the orbit of which a resonant pair of super-Earth-like planets has been announced. This detection was based on 62 radial velocity measurements obtained with the HARPS spectrograph (public data). Aims. We present a new planet search program that is using the HARPS spectrograph to search for Neptunes and super-Earths that orbit a sample of metal-poor FGK dwarfs. We then present a detailed analysis of 162 additional radial velocity measurements of HD?41248, obtained within this program, with the goal of confirming the existence of the proposed planetary system. Methods. We analysed the precise radial velocities, obtained with the HARPS spectrograph, together with several stellar activity diagnostics and line profile indicators. Results. A careful analysis shows no evidence for the planetary system. One of the signals, with a period of ~25?days, is shown to be related to the rotational period of the star, and is clearly seen in some of the activity proxies. We were unable to convincingly retrieve the remaining signal (P ~ 18?days) in the new dataset. Conclusions. We discuss possible causes for the complex (evolving) signals observed in the data of HD?41248, proposing that they might be explained by the appearance and disappearance of active regions on the surface of a star with strong differential rotation, or by a combination of the sparse data sampling and active region evolution. [ABSTRACT FROM AUTHOR]

Androgen action is exerted through the androgen receptor. The normal 46,XY genital virilization depends on androgen receptor gene expression, which is tissue specific, and requires normal androgen receptor mRNA levels in androgen sensitive tissues. Hypospadias is a frequent male genital abnormality, potentially related to reduced androgen sensitivity in genital tissues. The aim of this study was to compare, by quantitative real time PCR, the amount of androgen receptor mRNA in cells obtained from the urethral mucosa of patients with middle idiopathic hypospadias with the androgen receptor mRNA levels observed in control phimosis subjects with eutopic urethral opening. Prepubertal individuals were studied, including 41 controls and 17 hypospadias patients with mean (SD) ages of 4.7 (2.1) years and 4.0 (3.0) years, respectively. We observed significantly less androgen receptor mRNA in the urethral mucosa of patients with hypospadias than in the controls (p = 0.002). The correlation between the level of androgen receptor mRNA expression and the penile size was almost statistically significant only in hypospadias patients (r = 0.47; p = 0.053). We also established the number of CAG repeats in exon 1 of the androgen receptor gene by GeneScan analysis. No significant difference was observed in the number of CAG repeats when patients and controls were compared. A negative correlation between the CAG repeats and penile size was detected in patients with hypospadias, but not in controls. Our data suggest that a critical lower level of androgen receptor mRNA expression could be a determining factor in the development of middle hypospadias. [ABSTRACT FROM AUTHOR]

In this work, a semi-analytical model was developed to determine the contrast-to-noise ratio (CNR) and the normalized average glandular dose (\barDgN) in digital mammography, taking into account interactions up to second order. The optimal x-ray spectra were investigated using a Figure of Merit (FOM), for different breast thicknesses (3\ cm\leqL\leq 7\ cm) and glandularities (20% and 50%), anti-scatter grids types, considering a-Se and CsI image receptors. The anode/filter combinations evaluated were: Mo/Mo, Mo/Rh, Rh/Rh, and a W anode combined with k-edge filters (Mo, Ru, Rh, Pd, Ag, Cd, In and Sn), for tube potential between 23 and 35 kVp. Results demonstrate that the W-anode spectra combined with k-edge filters show a superior performance compared to the Mo/Mo combination traditionally employed in mammography, for all conditions analyzed. The optimum general x-ray spectra for 3 cm, 5 cm and 7 cm breasts are, respectively, W/Mo at 23 kVp, W/Pd at 28 kVp and W/Ag at 30 kVp. For the a-Se receptor, the optimum filter atomic number is one unit higher than that obtained for the CsI, while the optimal tube potentials are the same. The optimal tube potential was up to 1-2 kVp greater for a linear grid than that obtained without grid or with a cellular grid. For breast thicker than 7 cm, the optimum filter atomic number also increases by one unit for a linear grid. Compared to standard technique, the performance improvement of a W anode with proper k-edge filters is more noticeable for thicker and denser breasts, for a a-Se receptor and a linear grid. Finally, it was verified that the semi-analytical model implemented provided results of optimum x-ray spectra in a fast and simple way, with a good agreement with those obtained experimentally or by Monte Carlo simulation. [ABSTRACT FROM PUBLISHER]

Context. With the announced arrival of instruments such as ESPRESSO one can expect that several systematic noise sources on the measurement of precise radial velocity will become the limiting factor instead of photon noise. A stellar companion within the fiber is such a possible noise source. Aims. With this work we aim at characterizing the impact of a stellar companion within the fiber to radial velocity measurements made by fiber-fed spectrographs. We consider the contaminant star either to be part of a binary system whose primary star is the target star, or as a background/foreground star. Methods. To carry out our study, we used HARPS spectra, co-added the target with contaminant spectra, and then compared the resulting radial velocity with that obtained from the original target spectrum. We repeated this procedure and used different tunable knobs to reproduce the previously mentioned scenarios. Results. We find that the impact on the radial velocity calculation is a function of the difference between individual radial velocities, of the difference between target and contaminant magnitude, and also of their spectral types. For the worst-case scenario in which both target and contaminant star are well-centered on the fiber, the maximum contamination for a ɢ or Κ star may be higher than 10 cm s-1, on average, if the difference between target and contaminant magnitude is ▵ m < 10, and higher than 1 m/s if ▵ m < 8. If the target star is of spectral type M, ▵ m < 8 produces the same contamination of 10 cms-1, and a contamination may be higher than 1 m/s if ▵ m < 6. [ABSTRACT FROM AUTHOR]

Abstract: A series of 10 MIL-88B(Fe) iron(III) dicarboxylate MOFs wherein the organic linker is functionalized by a large variety of polar and apolar functional groups (–H, –Br, –F, –CF3, –CH3, –NH2, –NO2, –OH) was investigated as a potential carrier for encapsulating drugs, using the cosmetic amphiphilic caffeine as a model molecule. Encapsulation using impregnation followed by thermogravimetric analysis (TGA) and high performance liquid chromatography (HPLC) measurements to quantitatively estimate the caffeine uptake, have been first performed on the functionalized MIL-88B(Fe) samples. This set of experimental data was further used as an ideal platform to conduct a quantitative structure activity relationship approach based on multiple linear regression (MLR) method with the aim to find out the most relevant chemical and structural features of the MIL-88B(Fe) that significantly affect the therapeutic molecule uptake. Individual QSAR models showed that tuning the polarity and the H-donor capacity of the organic linker can enhance the caffeine encapsulation, suggesting that the functional groups serve as anchoring points for the drug molecule, consistent with previous conclusions drawn from molecular simulations performed on similar functionalized MOFs. Consensus modeling approach based on the selection of the most diverse individual models was also employed to build more representative QSAR models over the chemical space that could be further used to predict the drug encapsulation performance of the MOFs grafted by other functional groups. [Copyright &y& Elsevier]

Nutrient criteria as reference concentrations and trophic state boundaries are necessary for water management worldwide because anthropogenic eutrophication is a threat to the water uses. We compiled data on total phosphorus (TP), nitrogen (TN) and chlorophyll a (Chl a) from 17 subtropical reservoirs monitored from 2005-2009 in the São Paulo State (Brazil) to calculate reference concentrations through the trisection method (United States Environmental Protection Agency). By dividing our dataset into thirds we presented trophic state boundaries and frequency curves for the nutrient levels in water bodies with different enrichment conditions. TP and TN baseline concentrations (0.010 mg/L and 0.350 mg/L, respectively) were bracketed by ranges for temperate reservoirs available in the literature. We propose trophic state boundaries (upper limits for the oligotrophic category: 0.010 mg TP/L, 0.460 mg TN/L and 1.7 µg Chl a/L; for the mesotrophic: 0.030 mg TP/L, 0.820 mg TN/L and 9.0 µg Chl a/L). Through an example with a different dataset (from the Itupararanga Reservoir, Brazil), we encouraged the use of frequency curves to compare data from individual monitoring efforts with the expected concentrations in oligotrophic, mesotrophic and eutrophic regional systems. Such analysis might help designing recovery programs to reach targeted concentrations and mitigate the undesirable eutrophication symptoms in subtropical freshwaters. [ABSTRACT FROM AUTHOR]

Despite their importance for water management, long-term studies on trophic state are relatively scarce in subtropical reservoirs. We analyzed total phosphorus (TP), total nitrogen (TN) and chlorophyll a (Chi a) concentrations in the Billings Reservoir (Brazil) over time: Phase 1 (1977-1992, Tietê River water was pumped to Billings to increase energy generation, 100m3 s1); Phase 2 (19922007, Tiet O water was conveyed to Billings only in special cases for flood avoidance, 8 m3 s1); and Phase 3 (2007-2010, besides flood control, Billings received Tietê water treated by an in situ flotation system, 13 m3 5-1). We compared our results with data from 12 reservoirs to evaluate current (20052009) enrichment conditions. Phosphorus and nitrogen concentrations decreased (p <0.05, MANOVA) from Phase 1 to 2 and were stable thereafter. TN/TP ratios increment (1 977-2010) indicated shift from Nto P-limitation in the reservoir, affecting the phytoplankton. Nutrient levels in Billings are currently between the expected concentrations in mesotrophic and eutrophic reservoirs (0.03 mg L1

Pancreatic β-cell apoptosis is a key feature of diabetes mellitus and the mitochondrial pathway of apoptosis is a major mediator of β-cell death. We presently evaluated the role of the myeloid cell leukemia sequence 1 (Mcl-1), an antiapoptotic protein of the Bcl-2 family, in β-cells following exposure to well-defined β-cell death effectors, for example, pro-inflammatory cytokines, palmitate and chemical endoplasmic reticulum (ER) stressors. All cytotoxic stresses rapidly and preferentially decreased Mcl-1 protein expression as compared with the late effect observed on the other antiapoptotic proteins, Bcl-2 and Bcl-xL. This was due to ER stress-mediated inhibition of translation through eIF2α phosphorylation for palmitate and ER stressors and through the combined action of translation inhibition and JNK activation for cytokines. Knocking down Mcl-1 using small interference RNAs increased apoptosis and caspase-3 cleavage induced by cytokines, palmitate or thapsigargin, whereas Mcl-1 overexpression partly prevented Bax translocation to the mitochondria, cytochrome c release, caspase-3 cleavage and apoptosis induced by the β-cell death effectors. Altogether, our data suggest that Mcl-1 downregulation is a crucial event leading to β-cell apoptosis and provide new insights into the mechanisms linking ER stress and the mitochondrial intrinsic pathway of apoptosis. Mcl-1 is therefore an attractive target for the design of new strategies in the treatment of diabetes. [ABSTRACT FROM AUTHOR]