123 results on '"Ciccodicola, Alfredo"'
Search Results
2. GIPR expression is induced by thiazolidinediones in a PPARγ-independent manner and repressed by obesogenic stimuli
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Cataldi, Simona, Aprile, Marianna, Perfetto, Caterina, Angot, Brice, Cormont, Mireille, Ciccodicola, Alfredo, Tanti, Jean-Francois, and Costa, Valerio
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- 2023
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3. Transcriptional Regulation and Its Misregulation in Human Diseases.
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Casamassimi, Amelia, Ciccodicola, Alfredo, and Rienzo, Monica
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GENETIC transcription regulation , *MORPHOLOGY , *CIRCULAR RNA , *MOLECULAR genetics , *GENE expression , *RNA splicing , *NUCLEIC acids , *FORKHEAD transcription factors - Abstract
In this newly published study, the authors also identified aberrantly activated NKL genes, NKX2-3 and NKX2-4, in cell lines derived from two different AML subtypes, where they deregulate target genes involved in megakaryocytic and erythroid differentiation, thus providing the molecular basis to the classification of specific AML subtypes [[4]]. Transcriptional regulation is a critical biological process that allows the cell or an organism to respond to a variety of intra- and extracellular signals, to define cell identity during development, to maintain it throughout its lifetime, and to coordinate cellular activity. To this purpose, fifty female adult-onset asthma patients were categorized based on their exposure to building dampness and molds and other clinical parameters (inflammation, cytokine profile, etc.), together with gene signatures of nasal biopsies and peripheral blood mononuclear cells. An interesting study has analyzed the genetic structure and biological roles of I CLEC12A-MIR223HG i , a novel chimeric transcript produced through trans-splicing by the fusion of the cell surface receptor CLEC12A (C-Type Lectin Domain Family 12 Member A) and the miRNA-223 host gene ( I MIR223HG i ), first identified through transcriptome profiling of chronic myeloid leukemia (CML) patients. [Extracted from the article]
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- 2023
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4. Detrimental Effects of Bartonella henselae Are Counteracted by L-Arginine and Nitric Oxide in Human Endothelial Progenitor Cells
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Salvatore, Paola, Casamassimi, Amelia, Sommese, Linda, Fiorito, Carmela, Ciccodicola, Alfredo, Rossiello, Raffaele, Avallone, Bice, Grimaldi, Vincenzo, Costa, Valerio, Rienzo, Monica, Colicchio, Roberta, Williams-Ignarro, Sharon, Pagliarulo, Caterina, Prudente, Maria Evelina, Abbondanza, Ciro, Lamberti, Florentia, Baroni, Adone, Buommino, Elisabetta, Farzati, Bartolomeo, Tufano, Maria Antonietta, Ignarro, Louis Joseph, and Napoli, Claudio
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- 2008
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5. Nutritional genomics era: opportunities toward a genome-tailored nutritional regimen
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Costa, Valerio, Casamassimi, Amelia, and Ciccodicola, Alfredo
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- 2010
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6. Antigen delivery by filamentous bacteriophage fd displaying an anti-DEC-205 single-chain variable fragment confers adjuvanticity by triggering a TLR9-mediated immune response
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Sartorius, Rossella, DʼApice, Luciana, Trovato, Maria, Cuccaro, Fausta, Costa, Valerio, De Leo, Maria Giovanna, Marzullo, Vincenzo Manuel, Biondo, Carmelo, DʼAuria, Sabato, De Matteis, Maria Antonietta, Ciccodicola, Alfredo, and De Berardinis, Piergiuseppe
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- 2015
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7. Conserved Sequence-Tagged Sites: A Phylogenetic Approach to Genome Mapping
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Mazzarella, Richard, Montanaro, Vittorio, Kere, Juha, Reinbold, Rolland, Ciccodicola, Alfredo, D'Urso, Michele, and Schlessinger, David
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- 1992
8. Diabetic Retinopathy: Are lncRNAs New Molecular Players and Targets?
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Cataldi, Simona, Tramontano, Mariagiovanna, Costa, Valerio, Aprile, Marianna, and Ciccodicola, Alfredo
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DIABETIC retinopathy ,VASCULAR endothelial growth factors ,LINCRNA ,DRUG target ,NON-coding RNA ,HYPERGLYCEMIA - Abstract
The growing incidence of diabetes mellitus worldwide implies the increasing prevalence of several related macro- (e.g., hypertension and atherosclerosis) and micro-vascular (e.g., nephropathy and retinopathy) complications. Notably, diabetic retinopathy (DR) is the leading cause of blindness in older diabetic patients and can occur with different degrees of severity. Chronic hyperglycemia is the main determinant of the functional damage of retinal cells. The oxidative stress, inflammatory factors and vascular endothelial growth factor signaling have been widely reported as contributors of DR onset and progression, and an emerging role has been described for different classes of non-coding RNA, including several long non-coding RNAs (lncRNAs). Here, we report the main results of all research articles (i.e., 150) listed on PubMed database from 2014 to 2022 regarding the putative role of lncRNAs in DR, including small nucleolar RNA host genes (SNHGs). Particularly, in this review we describe all lncRNAs and SNHGs with altered expression in DR and related contexts, discussing their association with DR outcomes, their mechanism of action related to DR, the molecular/functional effects, as well as the biological and experimental contexts. Thus, herein we provide an overview of the current state of knowledge regarding the putative involvement of 50 lncRNAs and SNHGs in the pathogenesis of DR, highlighting their potential as therapeutic targets or biomarkers for improving the clinical management of DR. [ABSTRACT FROM AUTHOR]
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- 2022
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9. Hepatic Insulin Resistance in Hyperthyroid Rat Liver: Vitamin E Supplementation Highlights a Possible Role of ROS.
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Fasciolo, Gianluca, Napolitano, Gaetana, Aprile, Marianna, Cataldi, Simona, Costa, Valerio, Ciccodicola, Alfredo, Di Meo, Sergio, and Venditti, Paola
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VITAMIN E ,DIETARY supplements ,INSULIN resistance ,GLYCEMIC control ,BLOOD sugar ,LIVER - Abstract
Thyroid hormones are normally involved in glycaemic control, but their excess can lead to altered glucose metabolism and insulin resistance (IR). Since hyperthyroidism-linked increase in ROS results in tissue oxidative stress that is considered a hallmark of conditions leading to IR, it is conceivable a role of ROS in the onset of IR in hyperthyroidism. To verify this hypothesis, we evaluated the effects of vitamin E on thyroid hormone-induced oxidative damage, insulin resistance, and on gene expression of key molecules involved in IR in the rat liver. The factors involved in oxidative damage, namely the total content of ROS, the mitochondrial production of ROS, the activity of antioxidant enzymes, the in vitro susceptibility to oxidative stress, have been correlated to insulin resistance indices, such as insulin activation of hepatic Akt and plasma level of glucose, insulin and HOMA index. Our results indicate that increased levels of oxidative damage ROS content and production and susceptibility to oxidative damage, parallel increased fasting plasma level of glucose and insulin, reduced activation of Akt and increased activation of JNK. This last result suggests a role for JNK in the insulin resistance induced by hyperthyroidism. Furthermore, the variation of the genes Pparg, Ppara, Cd36 and Slc2a2 could explain, at least in part, the observed metabolic phenotypes. [ABSTRACT FROM AUTHOR]
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- 2022
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10. Filamin A is mutated in X-linked chronic idiopathic intestinal pseudo-obstruction with central nervous system involvement
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Gargiulo, Annagiusi, Auricchio, Renata, Barone, Maria Vittoria, Cotugno, Gabriella, Reardon, William, Milla, Peter J., Ballabio, Andrea, Ciccodicola, Alfredo, and Auricchio, Alberto
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Gene expression -- Research ,Fetal brain -- Genetic aspects ,Gene mutations -- Research ,Biological sciences - Abstract
The expression in murine fetal brain and intestine of 56 genes from the critical region is analyzed to select candidate genes for the disease. Results indicated that one affected male from a large CIIPX-affected kindred bears a 2-bp deletion in exon 2 of the FLNA gene that is present at the heterozygous state in the carrier females of the family.
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- 2007
11. Genetic and epigenetic alterations of RB2/p130 tumor suppressor gene in human sporadic retinoblastoma: implications for pathogenesis and therapeutic approach
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Tosi, Gian Marco, Trimarchi, Carmela, Macaluso, Marcella, La Sala, Dario, Ciccodicola, Alfredo, Lazzi, Stefano, Massaro-Giordano, Mina, Caporossi, Aldo, Giordano, Antonio, and Cinti, Caterina
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- 2005
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12. The DNA sequence of the human X chromosome
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Ross, Mark T., Grafham, Darren V., Coffey, Alison J., Scherer, Steven, McLay, Kirsten, Muzny, Donna, Platzer, Matthias, Howell, Gareth R., Burrows, Christine, Bird, Christine P., Frankish, Adam, Lovell, Frances L., Howe, Kevin L., Ashurst, Jennifer L., Fulton, Robert S., Sudbrak, Ralf, Wen, Gaiping, Jones, Matthew C., Hurles, Matthew E., Andrews, T. Daniel, Scott, Carol E., Searle, Stephen, Ramser, Juliane, Whittaker, Adam, Deadman, Rebecca, Carter, Nigel P., Hunt, Sarah E., Chen, Rui, Cree, Andrew, Gunaratne, Preethi, Havlak, Paul, Hodgson, Anne, Metzker, Michael L., Richards, Stephen, Scott, Graham, Steffen, David, Sodergren, Erica, Wheeler, David A., Worley, Kim C., Ainscough, Rachael, Ambrose, Kerrie D., Ansari-Lari, M. Ali, Aradhya, Swaroop, Ashwell, Robert I. S., Babbage, Anne K., Bagguley, Claire L., Ballabio, Andrea, Banerjee, Ruby, Barker, Gary E., Barlow, Karen F., Barrett, Ian P., Bates, Karen N., Beare, David M., Beasley, Helen, Beasley, Oliver, Beck, Alfred, Bethel, Graeme, Blechschmidt, Karin, Brady, Nicola, Bray-Allen, Sarah, Bridgeman, Anne M., Brown, Andrew J., Brown, Mary J., Bonnin, David, Bruford, Elspeth A., Buhay, Christian, Burch, Paula, Burford, Deborah, Burgess, Joanne, Burrill, Wayne, Burton, John, Bye, Jackie M., Carder, Carol, Carrel, Laura, Chako, Joseph, Chapman, Joanne C., Chavez, Dean, Chen, Ellson, Chen, Guan, Chen, Yuan, Chen, Zhijian, Chinault, Craig, Ciccodicola, Alfredo, Clark, Sue Y., Clarke, Graham, Clee, Chris M., Clegg, Sheila, Clerc-Blankenburg, Kerstin, Clifford, Karen, Cobley, Vicky, Cole, Charlotte G., Conquer, Jen S., Corby, Nicole, Connor, Richard E., David, Robert, Davies, Joy, Davis, Clay, Davis, John, Delgado, Oliver, DeShazo, Denise, Dhami, Pawandeep, Ding, Yan, Dinh, Huyen, Dodsworth, Steve, Draper, Heather, Dugan-Rocha, Shannon, Dunham, Andrew, Dunn, Matthew, Durbin, K. James, Dutta, Ireena, Eades, Tamsin, Ellwood, Matthew, Emery-Cohen, Alexandra, Errington, Helen, Evans, Kathryn L., Faulkner, Louisa, Francis, Fiona, Frankland, John, Fraser, Audrey E., Galgoczy, Petra, Gilbert, James, Gill, Rachel, Glockner, Gernot, Gregory, Simon G., Gribble, Susan, Griffiths, Coline, Grocock, Russell, Gu, Yanghong, Gwilliam, Rhian, Hamilton, Cerissa, Hart, Elizabeth A., Hawes, Alicia, Heath, Paul D., Heitmann, Katja, Hennig, Steffen, Hernandez, Judith, Hinzmann, Bernd, Ho, Sarah, Hoffs, Michael, Howden, Phillip J., Huckle, Elizabeth J., Hume, Jennifer, Hunt, Paul J., Hunt, Adrienne R., Isherwood, Judith, Jacob, Leni, Johnson, David, Jones, Sally, de Jong, Pieter J., Joseph, Shirin S., Keenan, Stephen, Kelly, Susan, Kershaw, Joanne K., Khan, Ziad, Kioschis, Petra, Klages, Sven, Knights, Andrew J., Kosiura, Anna, Kovar-Smith, Christie, Laird, Gavin K., Langford, Cordelia, Lawlor, Stephanie, Leversha, Margaret, Lewis, Lora, Liu, Wen, Lloyd, Christine, Lloyd, David M., Loulseged, Hermela, Loveland, Jane E., Lovell, Jamieson D., Lozado, Ryan, Lu, Jing, Lyne, Rachael, Ma, Jie, Maheshwari, Manjula, Matthews, Lucy H., McDowall, Jennifer, McLaren, Stuart, McMurray, Amanda, Meidl, Patrick, Meitinger, Thomas, Milne, Sarah, Miner, George, Mistry, Shailesh L., Morgan, Margaret, Morris, Sidney, Muller, Ines, Mullikin, James C., Nguyen, Ngoc, Nordsiek, Gabriele, Nyakatura, Gerald, O'Dell, Christopher N., Okwuonu, Geoffery, Palmer, Sophie, Pandian, Richard, Parker, David, Parrish, Julia, Pasternak, Shiran, Patel, Dina, Pearce, Alex V., Pearson, Danita M., Pelan, Sarah E., Perez, Lesette, Porter, Keith M., Ramsey, Yvonne, Reichwald, Kathrin, Rhodes, Susan, Ridler, Kerry A., Schlessinger, David, Schueler, Mary G., Sehra, Harminder K., Shaw-Smith, Charles, Shen, Hua, Sheridan, Elizabeth M., Shownkeen, Ratna, Skuce, Carl D., Smith, Michelle L., Sotheran, Elizabeth C., Steingruber, Helen E., Steward, Charles A., Storey, Roy, Swann, R. Mark, Swarbreck, David, Tabor, Paul E., Taudien, Stefan, Taylor, Tineace, Teague, Brian, Thomas, Karen, Thorpe, Andrea, Timms, Kirsten, Tracey, Alan, Trevanion, Steve, Tromans, Anthony C., d'Urso, Michele, Verduzco, Daniel, Villasana, Donna, Waldron, Lenee, Wall, Melanie, Wang, Qiaoyan, Warren, James, Warry, Georgina L., Wei, Xuehong, West, Anthony, Whitehead, Siobhan L., Whiteley, Mathew N., Wilkinson, Jane E., Willey, David L., Williams, Gabrielle, Williams, Leanne, Williamson, Angela, Williamson, Helen, Wilming, Laurens, Woodmansey, Rebecca L., Wray, Paul W., Yen, Jennifer, Zhang, Jingkun, Zhou, Jianling, Zoghbi, Huda, Zorilla, Sara, Buck, David, Reinhardt, Richard, Poustka, Annemarie, Rosenthal, Andre, Lehrach, Hans, Meindl, Alfons, Minx, Patrick J., Hillier, LaDeana W., Willard, Huntington F., Wilson, Richard K., Waterston, Robert H., Rice, Catherine M., Vaudin, Mark, Coulson, Alan, Nelson, David L., Weinstock, George, Sulston, John E., Durbin, Richard, Hubbard, Tim, Gibbs, Richard A., Beck, Stephan, Rogers, Jane, and Bentley, David R.
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Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Mark T. Ross (corresponding author) [1]; Darren V. Grafham [1]; Alison J. Coffey [1]; Steven Scherer [2]; Kirsten McLay [1]; Donna Muzny [2]; Matthias Platzer [3]; Gareth R. Howell [...]
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- 2005
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13. Integrated Network Pharmacology Approach for Drug Combination Discovery: A Multi-Cancer Case Study.
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Federico, Antonio, Fratello, Michele, Scala, Giovanni, Möbus, Lena, Pavel, Alisa, del Giudice, Giusy, Ceccarelli, Michele, Costa, Valerio, Ciccodicola, Alfredo, Fortino, Vittorio, Serra, Angela, and Greco, Dario
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DRUG repositioning ,RESEARCH ,COMBINATION drug therapy ,PHARMACOLOGY ,CASE studies ,GENOMES ,TUMORS ,PACLITAXEL - Abstract
Simple Summary: Current treatments for complex diseases, including cancer, are generally characterized by high toxicity due to their low selectivity for target cells. Moreover, patients often develop drug resistance, hence becoming less sensitive to the therapy. For this reason, novel, improved, and more specific pharmacological therapies are needed. The high cost and the time required to develop new drugs poses the attention on the development of computational methods for drug repositioning and combination therapy prediction. In this study, we developed an integrated network pharmacology framework that combines mechanistic and chemocentric approaches in order to predict potential drug combinations for cancer therapy. We applied our paradigm in five cancer types, which we used as case studies. Our strategy can be applied to the study of any complex disease by guiding the prioritization of drug combinations. Despite remarkable efforts of computational and predictive pharmacology to improve therapeutic strategies for complex diseases, only in a few cases have the predictions been eventually employed in the clinics. One of the reasons behind this drawback is that current predictive approaches are based only on the integration of molecular perturbation of a certain disease with drug sensitivity signatures, neglecting intrinsic properties of the drugs. Here we integrate mechanistic and chemocentric approaches to drug repositioning by developing an innovative network pharmacology strategy. We developed a multilayer network-based computational framework integrating perturbational signatures of the disease as well as intrinsic characteristics of the drugs, such as their mechanism of action and chemical structure. We present five case studies carried out on public data from The Cancer Genome Atlas, including invasive breast cancer, colon adenocarcinoma, lung squamous cell carcinoma, hepatocellular carcinoma and prostate adenocarcinoma. Our results highlight paclitaxel as a suitable drug for combination therapy for many of the considered cancer types. In addition, several non-cancer-related genes representing unusual drug targets were identified as potential candidates for pharmacological treatment of cancer. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Is PPARG the key gene in diabetic retinopathy?
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Costa, Valerio and Ciccodicola, Alfredo
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- 2012
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15. Multiple pathogenic and benign genomic rearrangements occur at a 35 kb duplication involving the NEMO and LAGE2 genes
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Aradhya, Swaroop, Bardaro, Tiziana, Galgóczy, Petra, Yamagata, Takanori, Esposito, Teresa, Patlan, Henry, Ciccodicola, Alfredo, Munnich, Arnold, Kenwrick, Sue, Platzer, Matthias, D’Urso, Michele, and Nelson, David L.
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- 2001
16. PPARγ and Diabetes: Beyond the Genome and Towards Personalized Medicine.
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Cataldi, Simona, Costa, Valerio, Ciccodicola, Alfredo, and Aprile, Marianna
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Purpose Of Review: Full and partial synthetic agonists targeting the transcription factor PPARγ are contained in FDA-approved insulin-sensitizing drugs and used for the treatment of metabolic syndrome-related dysfunctions. Here, we discuss the association between PPARG genetic variants and drug efficacy, as well as the role of alternative splicing and post-translational modifications as contributors to the complexity of PPARγ signaling and to the effects of synthetic PPARγ ligands.Recent Findings: PPARγ regulates the transcription of several target genes governing adipocyte differentiation and glucose and lipid metabolism, as well as insulin sensitivity and inflammatory pathways. These pleiotropic functions confer great relevance to PPARγ in physiological regulation of whole-body metabolism, as well as in the etiology of metabolic disorders. Accordingly, PPARG gene mutations, nucleotide variations, and post-translational modifications have been associated with adipose tissue disorders and the related risk of insulin resistance and type 2 diabetes (T2D). Moreover, PPARγ alternative splicing isoforms-generating dominant-negative isoforms mainly expressed in human adipose tissue-have been related to impaired PPARγ activity and adipose tissue dysfunctions. Thus, multiple regulatory levels that contribute to PPARγ signaling complexity may account for the beneficial as well as adverse effects of PPARγ agonists. Further targeted analyses, taking into account all these aspects, are needed for better deciphering the role of PPARγ in human pathophysiology, especially in insulin resistance and T2D. The therapeutic potential of full and partial PPARγ synthetic agonists underlines the clinical significance of this nuclear receptor. PPARG mutations, polymorphisms, alternative splicing isoforms, and post-translational modifications may contribute to the pathogenesis of metabolic disorders, also influencing the responsiveness of pharmacological therapy. Therefore, in the context of the current evidence-based trend to personalized diabetes management, we highlight the need to decipher the intricate regulation of PPARγ signaling to pave the way to tailored therapies in patients with insulin resistance and T2D. [ABSTRACT FROM AUTHOR]- Published
- 2021
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17. Mutation analysis of the DKC1 gene in incontinentia pigmenti
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HEISS, NINA S, POUSTKA, ANNEMARIE, KNIGHT, STUART W, ARADHYA, SWAROOP, NELSON, DAVID L, LEWIS, RICHARD A, ESPOSITO, TERESA, CICCODICOLA, ALFREDO, D'URSO, MICHELE, SMAHI, ASMAE, HEUERTZ, SOLANGE, MUNNICH, ARNOLD, VABRES, PIERRE, WOFFENDIN, HAYLEY, and KENWRICK, SUSAN
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- 1999
18. Impairment of circulating endothelial progenitors in Down syndrome
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Costa Valerio, Sommese Linda, Casamassimi Amelia, Colicchio Roberta, Angelini Claudia, Marchesano Valentina, Milone Lara, Farzati Bartolomeo, Giovane Alfonso, Fiorito Carmela, Rienzo Monica, Picardi Marco, Avallone Bice, Marco Corsi Massimiliano, Sarubbi Berardo, Calabrò Raffaele, Salvatore Paola, Ciccodicola Alfredo, and Napoli Claudio
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Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Pathological angiogenesis represents a critical issue in the progression of many diseases. Down syndrome is postulated to be a systemic anti-angiogenesis disease model, possibly due to increased expression of anti-angiogenic regulators on chromosome 21. The aim of our study was to elucidate some features of circulating endothelial progenitor cells in the context of this syndrome. Methods Circulating endothelial progenitors of Down syndrome affected individuals were isolated, in vitro cultured and analyzed by confocal and transmission electron microscopy. ELISA was performed to measure SDF-1α plasma levels in Down syndrome and euploid individuals. Moreover, qRT-PCR was used to quantify expression levels of CXCL12 gene and of its receptor in progenitor cells. The functional impairment of Down progenitors was evaluated through their susceptibility to hydroperoxide-induced oxidative stress with BODIPY assay and the major vulnerability to the infection with human pathogens. The differential expression of crucial genes in Down progenitor cells was evaluated by microarray analysis. Results We detected a marked decrease of progenitors' number in young Down individuals compared to euploid, cell size increase and some major detrimental morphological changes. Moreover, Down syndrome patients also exhibited decreased SDF-1α plasma levels and their progenitors had a reduced expression of SDF-1α encoding gene and of its membrane receptor. We further demonstrated that their progenitor cells are more susceptible to hydroperoxide-induced oxidative stress and infection with Bartonella henselae. Further, we observed that most of the differentially expressed genes belong to angiogenesis, immune response and inflammation pathways, and that infected progenitors with trisomy 21 have a more pronounced perturbation of immune response genes than infected euploid cells. Conclusions Our data provide evidences for a reduced number and altered morphology of endothelial progenitor cells in Down syndrome, also showing the higher susceptibility to oxidative stress and to pathogen infection compared to euploid cells, thereby confirming the angiogenesis and immune response deficit observed in Down syndrome individuals.
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- 2010
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19. Autosomal recessive familial exudative vitreoretinopathy: evidence for genetic heterogeneity
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de Crecchio, Giuseppe, Simonelli, Francesca, Nunziata, Giuseppe, Mazzeo, Salvatore, Greco, Giovanni Maria, Rinaldi, Ernesto, Ventruto, Valerio, Ciccodicola, Alfredo, Miano, Maria Giuseppina, Testa, Francesco, Curci, Anna, D'Urso, Michele, Rinaldi, Maria Michela, Cavaliere, Maria Luigia, and Castelluccio, Pia
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- 1998
20. Ocular signs associated with a rhodopsin mutation (Cys-167→Arg) in a family with autosomal dominant retinitis pigmentosa
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SIMONELLI, FRANCESCA, RINALDI, MARIO, NESTI, ANNA, TESTA, FRANCESCO, RINALDI, ERNESTO, CICCODICOLA, ALFREDO, FLAGIELLO, LUISA, MIANO, MARIA GIUSEPPINA, VENTRUTO, VALERIO, and D'URSO, MICHELE
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- 1998
21. DDX11L: a novel transcript family emerging from human subtelomeric regions
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D'Urso Michele, Matarazzo Maria R, Gianfrancesco Fernando, Roberto Roberta, Casamassimi Amelia, Costa Valerio, D'Esposito Maurizio, Rocchi Mariano, and Ciccodicola Alfredo
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The subtelomeric regions of human chromosomes exhibit an extraordinary plasticity. To date, due to the high GC content and to the presence of telomeric repeats, the subtelomeric sequences are underrepresented in the genomic libraries and consequently their sequences are incomplete in the finished human genome sequence, and still much remains to be learned about subtelomere organization, evolution and function. Indeed, only in recent years, several studies have disclosed, within human subtelomeres, novel gene family members. Results During a project aimed to analyze genes located in the telomeric region of the long arm of the human X chromosome, we have identified a novel transcript family, DDX11L, members of which map to 1pter, 2q13/14.1, 2qter, 3qter, 6pter, 9pter/9qter, 11pter, 12pter, 15qter, 16pter, 17pter, 19pter, 20pter/20qter, Xpter/Xqter and Yqter. Furthermore, we partially sequenced the underrepresented subtelomeres of human chromosomes showing a common evolutionary origin. Conclusion Our data indicate that an ancestral gene, originated as a rearranged portion of the primate DDX11 gene, and propagated along many subtelomeric locations, is emerging within subtelomeres of human chromosomes, defining a novel gene family. These findings support the possibility that the high plasticity of these regions, sites of DNA exchange among different chromosomes, could trigger the emergence of new genes.
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- 2009
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22. Investigation of Gamma-aminobutyric acid (GABA) A receptors genes and migraine susceptibility
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Ciccodicola Alfredo, Colson Natalie J, Lea Rod A, Esposito Teresa, Fernandez Francesca, Gianfrancesco Fernando, and Griffiths Lyn R
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Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Migraine is a neurological disorder characterized by recurrent attacks of severe headache, affecting around 12% of Caucasian populations. It is well known that migraine has a strong genetic component, although the number and type of genes involved is still unclear. Prior linkage studies have reported mapping of a migraine gene to chromosome Xq 24–28, a region containing a cluster of genes for GABA A receptors (GABRE, GABRA3, GABRQ), which are potential candidate genes for migraine. The GABA neurotransmitter has been implicated in migraine pathophysiology previously; however its exact role has not yet been established, although GABA receptors agonists have been the target of therapeutic developments. The aim of the present research is to investigate the role of the potential candidate genes reported on chromosome Xq 24–28 region in migraine susceptibility. In this study, we have focused on the subunit GABA A receptors type ε (GABRE) and type θ (GABRQ) genes and their involvement in migraine. Methods We have performed an association analysis in a large population of case-controls (275 unrelated Caucasian migraineurs versus 275 controls) examining a set of 3 single nucleotide polymorphisms (SNPs) in the coding region (exons 3, 5 and 9) of the GABRE gene and also the I478F coding variant of the GABRQ gene. Results Our study did not show any association between the examined SNPs in our test population (P > 0.05). Conclusion Although these particular GABA receptor genes did not show positive association, further studies are necessary to consider the role of other GABA receptor genes in migraine susceptibility.
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- 2008
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23. Characterization of a novel polymorphism in PPARG regulatory region associated with type 2 diabetes and diabetic retinopathy in Italy
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Costa, Valerio, Casamassimi, Amelia, Esposito, Katherine, Villani, Angela, Capone, Mariaelena, Iannella, Rosa, Schisano, Bruno, Ciotola, Miryam, Palo, Carmen Di, Corrado, Feliciantonia Capone, Santangelo, Franco, Giugliano, Dario, and Ciccodicola, Alfredo
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Genetic aspects ,Research ,Diabetic retinopathy -- Genetic aspects -- Research ,Type 2 diabetes -- Genetic aspects -- Research ,Genetic polymorphisms -- Research -- Genetic aspects - Abstract
1. Introduction Peroxisome proliferator-activated receptor gamma (PPARG) gene is a member of nuclear hormone receptor superfamily, and it regulates the transcription of several genes involved in glucose metabolism, adipocyte differentiation, [...], Peroxisome proliferator-activated receptor gamma polymorphisms have been widely associated with type 2 diabetes, although their role in the pathogenesis of vascular complications is not yet demonstrated. In this study, a cohort of 211 type 2 diabetes, 205 obese, and 254 control individuals was genotyped for Pro12Ala, C1431T, C-2821T polymorphisms, and for a newly identified polymorphism (A-2819G). The above-mentioned polymorphisms were analyzed by gene-specific PCR and direct sequencing of all samples. A significant difference was found for -2819G frequency when patients with type 2 diabetes--particularly diabetic women with the proliferative retinopathy--were compared with healthy control individuals. In conclusion, we identified a novel polymorphism, A-2819G, in PPARG gene, and we found it to be associated with type 2 diabetes and proliferative retinopathy in diabetic females. In the analyzed population, this variant represents a genetic risk factor for developing the diabetic retinopathy, whereas Pro12Ala and C1431T do not. Copyright [C] 2009 Valerio Costa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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- 2009
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24. RBPMetaDB: a comprehensive annotation of mouse RNA-Seq datasets with perturbations of RNA-binding proteins.
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Li, Jin, Deng, Su-Ping, Vieira, Jacob, Thomas, James, Costa, Valerio, Tseng, Ching-San, Ivankovic, Franjo, Ciccodicola, Alfredo, and Yu, Peng
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RNA sequencing ,RNA-binding proteins ,LABORATORY mice - Abstract
RNA-binding proteins (RBPs) may play a critical role in gene regulation in various diseases or biological processes by controlling post-transcriptional events such as polyadenylation, splicing and mRNA stabilization via binding activities to RNA molecules. Owing to the importance of RBPs in gene regulation, a great number of studies have been conducted, resulting in a large amount of RNA-Seq datasets. However, these datasets usually do not have structured organization of metadata, which limits their potentially wide use. To bridge this gap, the metadata of a comprehensive set of publicly available mouse RNA-Seq datasets with perturbed RBPs were collected and integrated into a database called RBPMetaDB. This database contains 292 mouse RNA-Seq datasets for a comprehensive list of 187 RBPs. These RBPs account for only ∼10% of all known RBPs annotated in Gene Ontology, indicating that most are still unexplored using high-throughput sequencing. This negative information provides a great pool of candidate RBPs for biologists to conduct future experimental studies. In addition, we found that DNA-binding activities are significantly enriched among RBPs in RBPMetaDB, suggesting that prior studies of these DNA- and RNA-binding factors focus more on DNA-binding activities instead of RNA-binding activities. This result reveals the opportunity to efficiently reuse these data for investigation of the roles of their RNA-binding activities. A web application has also been implemented to enable easy access and wide use of RBPMetaDB. It is expected that RBPMetaDB will be a great resource for improving understanding of the biological roles of RBPs. Database URL: http://rbpmetadb.yubiolab.org [ABSTRACT FROM AUTHOR]
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- 2018
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25. Heart failure: Pilot transcriptomic analysis of cardiac tissue by RNA-sequencing.
- Author
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Schiano, Concetta, Costa, Valerio, Aprile, Marianna, Grimaldi, Vincenzo, Maiello, Ciro, Esposito, Roberta, Soricelli, Andrea, Colantuoni, Vittorio, Donatelli, Francesco, Ciccodicola, Alfredo, and Napoli, Claudio
- Published
- 2017
- Full Text
- View/download PDF
26. Transcriptome Profiling in Human Diseases: New Advances and Perspectives.
- Author
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Casamassimi, Amelia, Federico, Antonio, Rienzo, Monica, Esposito, Sabrina, and Ciccodicola, Alfredo
- Subjects
PATHOLOGY ,RNA sequencing ,RNA analysis ,NON-coding RNA ,CATALYTIC RNA - Abstract
In the last decades, transcriptome profiling has been one of the most utilized approaches to investigate human diseases at the molecular level. Through expression studies, many molecular biomarkers and therapeutic targets have been found for several human pathologies. This number is continuously increasing thanks to total RNA sequencing. Indeed, this new technology has completely revolutionized transcriptome analysis allowing the quantification of gene expression levels and allele-specific expression in a single experiment, as well as to identify novel genes, splice isoforms, fusion transcripts, and to investigate the world of non-coding RNA at an unprecedented level. RNA sequencing has also been employed in important projects, like ENCODE (Encyclopedia of the regulatory elements) and TCGA (The Cancer Genome Atlas), to provide a snapshot of the transcriptome of dozens of cell lines and thousands of primary tumor specimens. Moreover, these studies have also paved the way to the development of data integration approaches in order to facilitate management and analysis of data and to identify novel disease markers and molecular targets to use in the clinics. In this scenario, several ongoing clinical trials utilize transcriptome profiling through RNA sequencing strategies as an important instrument in the diagnosis of numerous human pathologies. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
27. Pan-Cancer Mutational and Transcriptional Analysis of the Integrator Complex.
- Author
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Federico, Antonio, Rienzo, Monica, Abbondanza, Ciro, Costa, Valerio, Ciccodicola, Alfredo, and Casamassimi, Amelia
- Subjects
CANCER genetics ,GENETIC mutation ,SOMATIC mutation ,GENETIC code ,GENETIC transcription ,GENE expression profiling - Abstract
The integrator complex has been recently identified as a key regulator of RNA Polymerase II-mediated transcription, with many functions including the processing of small nuclear RNAs, the pause-release and elongation of polymerase during the transcription of protein coding genes, and the biogenesis of enhancer derived transcripts. Moreover, some of its components also play a role in genome maintenance. Thus, it is reasonable to hypothesize that their functional impairment or altered expression can contribute to malignancies. Indeed, several studies have described the mutations or transcriptional alteration of some Integrator genes in different cancers. Here, to draw a comprehensive pan-cancer picture of the genomic and transcriptomic alterations for the members of the complex, we reanalyzed public data from The Cancer Genome Atlas. Somatic mutations affecting Integrator subunit genes and their transcriptional profiles have been investigated in about 11,000 patients and 31 tumor types. A general heterogeneity in the mutation frequencies was observed, mostly depending on tumor type. Despite the fact that we could not establish them as cancer drivers, INTS7 and INTS8 genes were highly mutated in specific cancers. A transcriptome analysis of paired (normal and tumor) samples revealed that the transcription of INTS7, INTS8, and INTS13 is significantly altered in several cancers. Experimental validation performed on primary tumors confirmed these findings. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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- View/download PDF
28. SFMetaDB: a comprehensive annotation of mouse RNA splicing factor RNA-Seq datasets.
- Author
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Jin Li, Ching-San Tseng, Federico, Antonio, Ivankovic, Franjo, Yi-Shuian Huang, Ciccodicola, Alfredo, Swanson, Maurice S., and Peng Yu
- Abstract
Although the number of RNA-Seq datasets deposited publicly has increased over the past few years, incomplete annotation of the associated metadata limits their potential use. Because of the importance of RNA splicing in diseases and biological processes, we constructed a database called SFMetaDB by curating datasets related with RNA splicing factors. Our effort focused on the RNA-Seq datasets in which splicing factors were knocked-down, knocked-out or over-expressed, leading to 75 datasets corresponding to 56 splicing factors. These datasets can be used in differential alternative splicing analysis for the identification of the potential targets of these splicing factors and other functional studies. Surprisingly, only ~15% of all the splicing factors have been studied by loss- or gain-of-function experiments using RNA-Seq. In particular, splicing factors with domains from a few dominant Pfam domain families have not been studied. This suggests a significant gap that needs to be addressed to fully elucidate the splicing regulatory landscape. Indeed, there are already mouse models available for ~20 of the unstudied splicing factors, and it can be a fruitful research direction to study these splicing factors in vitro and in vivo using RNA-Seq. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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29. E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells.
- Author
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Trovato, Maria, Maurano, Francesco, D'Apice, Luciana, Costa, Valerio, Sartorius, Rossella, Cuccaro, Fausta, McBurney, Sean P., Krebs, Shelly J., Prisco, Antonella, Ciccodicola, Alfredo, Rossi, Mauro, Haigwood, Nancy L., and De Berardinis, Piergiuseppe
- Subjects
VACCINES ,BIOLOGICALS ,IMMUNE response ,IMMUNOLOGY ,DENDRITIC cells - Abstract
Background: The E2 multimeric scaffold represents a powerful delivery system able to elicit robust humoral and cellular immune responses upon systemic administrations. Here recombinant E2 scaffold displaying the third variable loop of HIV-1 Envelope gp120 glycoprotein was administered via mucosa, and the mucosal and systemic immune responses were analysed. To gain further insights into the molecular mechanisms that orchestrate the immune response upon E2 vaccination, we analysed the transcriptome profile of dendritic cells (DCs) exposed to the E2 scaffold with the aim to define a specific gene expression signature for E2-primed immune responses. Results: The in vivo immunogenicity and the potential of E2 scaffold as a mucosal vaccine candidate were investigated in BALB/c mice vaccinated via the intranasal route. Fecal and systemic antigen-specific IgA antibodies, cytokine-producing CD4
+ and CD8+ cells were induced assessing the immunogenicity of E2 particles via intranasal administration. The cytokine analysis identified a mixed T-helper cell response, while the systemic antibody response showed a prevalence of IgG1 isotype indicative of a polarized Th2-type immune response. RNA-Sequencing analysis revealed that E2 scaffold up-regulates in DCs transcriptional regulators of the Th2-polarizing cell response, defining a type 2 DC transcriptomic signature. Conclusions: The current study provides experimental evidence to the possible application of E2 scaffold as antigen delivery system for mucosal immunization and taking advantages of genome-wide approach dissects the type of response induced by E2 particles. [ABSTRACT FROM AUTHOR]- Published
- 2016
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30. Computational Analysis of Single Nucleotide Polymorphisms Associated with Altered Drug Responsiveness in Type 2 Diabetes.
- Author
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Costa, Valerio, Federico, Antonio, Pollastro, Carla, Ziviello, Carmela, Cataldi, Simona, Formisano, Pietro, and Ciccodicola, Alfredo
- Subjects
TYPE 2 diabetes ,SINGLE nucleotide polymorphisms ,PHARMACOGENOMICS ,INDIVIDUALIZED medicine ,RNA sequencing - Abstract
Type 2 diabetes (T2D) is one of the most frequent mortality causes in western countries, with rapidly increasing prevalence. Anti-diabetic drugs are the first therapeutic approach, although many patients develop drug resistance. Most drug responsiveness variability can be explained by genetic causes. Inter-individual variability is principally due to single nucleotide polymorphisms, and differential drug responsiveness has been correlated to alteration in genes involved in drug metabolism (CYP2C9) or insulin signaling (IRS1, ABCC8, KCNJ11 and PPARG). However, most genome-wide association studies did not provide clues about the contribution of DNA variations to impaired drug responsiveness. Thus, characterizing T2D drug responsiveness variants is needed to guide clinicians toward tailored therapeutic approaches. Here, we extensively investigated polymorphisms associated with altered drug response in T2D, predicting their effects in silico. Combining different computational approaches, we focused on the expression pattern of genes correlated to drug resistance and inferred evolutionary conservation of polymorphic residues, computationally predicting the biochemical properties of polymorphic proteins. Using RNA-Sequencing followed by targeted validation, we identified and experimentally confirmed that two nucleotide variations in the CAPN10 gene-currently annotated as intronic-fall within two new transcripts in this locus. Additionally, we found that a Single Nucleotide Polymorphism (SNP), currently reported as intergenic, maps to the intron of a new transcript, harboring CAPN10 and GPR35 genes, which undergoes non-sense mediated decay. Finally, we analyzed variants that fall into non-coding regulatory regions of yet underestimated functional significance, predicting that some of them can potentially affect gene expression and/or post-transcriptional regulation of mRNAs affecting the splicing. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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- View/download PDF
31. Alternative Splicing in Adhesion- and Motility-Related Genes in Breast Cancer.
- Author
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Aversa, Rosanna, Sorrentino, Anna, Esposito, Roberta, Ambrosio, Maria Rosaria, Amato, Angela, Zambelli, Alberto, Ciccodicola, Alfredo, D'Apice, Luciana, and Costa, Valerio
- Subjects
TUMORS ,BREAST cancer research ,SEMAPHORINS ,RNA splicing ,METASTASIS - Abstract
Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations—in human breast cell lines and breast tumor biopsies—we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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- View/download PDF
32. The “next-generation” knowledge of papillary thyroid carcinoma.
- Author
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Costa, Valerio, Esposito, Roberta, Pallante, Pierlorenzo, Ciccodicola, Alfredo, and Fusco, Alfredo
- Published
- 2015
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33. Pharmacogenomics of Drug Response in Type 2 Diabetes: Toward the Definition of Tailored Therapies?
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Pollastro, Carla, Ziviello, Carmela, Costa, Valerio, and Ciccodicola, Alfredo
- Subjects
TYPE 2 diabetes treatment ,PEOPLE with diabetes ,PHARMACOGENOMICS ,MORTALITY ,DISEASE prevalence ,DRUG resistance - Abstract
Type 2 diabetes is one of the major causes of mortality with rapidly increasing prevalence. Pharmacological treatment is the first recommended approach after failure in lifestyle changes. However, a significant number of patients shows—or develops along time and disease progression—drug resistance. In addition, not all type 2 diabetic patients have the same responsiveness to drug treatment. Despite the presence of nongenetic factors (hepatic, renal, and intestinal), most of such variability is due to genetic causes. Pharmacogenomics studies have described association between single nucleotide variations and drug resistance, even though there are still conflicting results. To date, the most reliable approach to investigate allelic variants is Next-Generation Sequencing that allows the simultaneous analysis, on a genome-wide scale, of nucleotide variants and gene expression. Here, we review the relationship between drug responsiveness and polymorphisms in genes involved in drug metabolism (CYP2C9) and insulin signaling (ABCC8, KCNJ11, and PPARG). We also highlight the advancements in sequencing technologies that to date enable researchers to perform comprehensive pharmacogenomics studies. The identification of allelic variants associated with drug resistance will constitute a solid basis to establish tailored therapeutic approaches in the treatment of type 2 diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
34. Novel Transcription Factor Variants through RNA-Sequencing: The Importance of Being "Alternative".
- Author
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Scarpato, Margherita, Federico, Antonio, Ciccodicola, Alfredo, and Costa, Valerio
- Subjects
TRANSCRIPTION factors ,RNA sequencing ,ALTERNATIVE RNA splicing ,KRUPPEL-like factors ,GENETIC transcription ,POLYMERASE chain reaction - Abstract
Alternative splicing is a pervasive mechanism of RNA maturation in higher eukaryotes, which increases proteomic diversity and biological complexity. It has a key regulatory role in several physiological and pathological states. The diffusion of Next Generation Sequencing, particularly of RNA-Sequencing, has exponentially empowered the identification of novel transcripts revealing that more than 95% of human genes undergo alternative splicing. The highest rate of alternative splicing occurs in transcription factors encoding genes, mostly in Krüppel-associated box domains of zinc finger proteins. Since these molecules are responsible for gene expression, alternative splicing is a crucial mechanism to "regulate the regulators". Indeed, different transcription factors isoforms may have different or even opposite functions. In this work, through a targeted re-analysis of our previously published RNA-Sequencing datasets, we identified nine novel transcripts in seven transcription factors genes. In silico analysis, combined with RT-PCR, cloning and Sanger sequencing, allowed us to experimentally validate these new variants. Through computational approaches we also predicted their novel structural and functional properties. Our findings indicate that alternative splicing is a major determinant of transcription factor diversity, confirming that accurate analysis of RNA-Sequencing data can reliably lead to the identification of novel transcripts, with potentially new functions. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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- View/download PDF
35. AnaLysis of Expression on human chromosome 21, ALE-HSA21: a pilot integrated web resource.
- Author
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Scarpato, Margherita, Esposito, Roberta, Evangelista, Daniela, Aprile, Marianna, Ambrosio, Maria Rosaria, Angelini, Claudia, Ciccodicola, Alfredo, and Costa, Valerio
- Abstract
Transcriptome studies have shown the pervasive nature of transcription, demonstrating almost all the genes undergo alternative splicing. Accurately annotating all transcripts of a gene is crucial. It is needed to understand the impact of mutations on phenotypes, to shed light on genetic and epigenetic regulation of mRNAs and more generally to widen our knowledge about cell functionality and tissue diversity. RNA-sequencing (RNA-Seq), and the other applications of the next-generation sequencing, provides precious data to improve annotations' accuracy, simultaneously creating issues related to the variety, complexity and the size of produced data. In this ‘scenario’, the lack of user-friendly resources, easily accessible to researchers with low skills in bioinformatics, makes difficult to retrieve complete information about one or few genes without browsing a jungle of databases. Concordantly, the increasing amount of data from ‘omics’ technologies imposes to develop integrated databases merging different data formats coming from distinct but complementary sources. In light of these considerations, and given the wide interest in studying Down syndrome—a genetic condition due to the trisomy of human chromosome 21 (HSA21)—we developed an integrated relational database and a web interface, named ALE-HSA21 (AnaLysis of Expression on HSA21), accessible at http://bioinfo.na.iac.cnr.it/ALE-HSA21. This comprehensive and user-friendly web resource integrates—for all coding and noncoding transcripts of chromosome 21—existing gene annotations and transcripts identified de novo through RNA-Seq analysis with predictive computational analysis of regulatory sequences. Given the role of noncoding RNAs and untranslated regions of coding genes in key regulatory mechanisms, ALE-HSA21 is also an interesting web-based platform to investigate such processes. The ‘transcript-centric’ and easily-accessible nature of ALE-HSA21 makes this resource a valuable tool to rapidly retrieve data at the isoform level, rather than at gene level, useful to investigate any disease, molecular pathway or cell process involving chromosome 21 genes. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
36. RNA-Seq and human complex diseases: recent accomplishments and future perspectives.
- Author
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Costa, Valerio, Aprile, Marianna, Esposito, Roberta, and Ciccodicola, Alfredo
- Subjects
HUMAN genome ,GENETIC research ,NUCLEOTIDE sequence ,NEUROBEHAVIORAL disorders ,NEURODEGENERATION ,GENETIC mutation - Abstract
The availability of the human genome sequence has allowed identification of disease-causing mutations in many Mendelian disorders, and detection of significant associations of nucleotide polymorphisms to complex diseases and traits. Despite these progresses, finding the causative variations for most of the common diseases remains a complex task. Several studies have shown gene expression analyses provide a quite unbiased way to investigate complex traits and common disorders' pathogenesis. Therefore, whole-transcriptome analysis is increasingly acquiring a key role in the knowledge of mechanisms responsible for complex diseases. Hybridization- and tag-based technologies have elucidated the involvement of multiple genes and pathways in pathological conditions, providing insights into the expression of thousand of coding and noncoding RNAs, such as microRNAs. However, the introduction of Next-Generation Sequencing, particularly of RNA-Seq, has overcome some drawbacks of previously used technologies. Identifying, in a single experiment, potentially novel genes/exons and splice isoforms, RNA editing, fusion transcripts and allele-specific expression are some of its advantages. RNA-Seq has been fruitfully applied to study cancer and host-pathogens interactions, and it is taking first steps for studying neurodegenerative diseases (ND) as well as neuropsychiatric diseases. In addition, it is emerging as a very powerful tool to study quantitative trait loci associated with gene expression in complex diseases. This paper provides an overview on gene expression profiling of complex diseases, with emphasis on RNA-Seq, its advantages over conventional technologies for studying cancer and ND, and for linking nucleotide variations to gene expression changes, also discussing its limitations. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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37. Evidence of Bacteroides fragilis Protection from Bartonella henselae-Induced Damage.
- Author
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Sommese, Linda, Pagliuca, Chiara, Avallone, Bice, Ippolito, Rossana, Casamassimi, Amelia, Costa, Valerio, Colicchio, Roberta, Cerciello, Raimondo, D'Armiento, Maria, Scarpato, Margherita, Giovane, Alfonso, Pastore, Gabiria, Infante, Teresa, Ciccodicola, Alfredo, Fiorito, Carmela, D'Armiento, Francesco Paolo, Salvatore, Paola, and Napoli, Claudio
- Subjects
ISCHEMIA ,REPERFUSION injury ,KIDNEY failure ,MORTALITY ,BLOOD flow ,CYTOLOGY - Abstract
Bartonella henselae is able to internalize endothelial progenitor cells (EPCs), which are resistant to the infection of other common pathogens. Bacteroides fragilis is a gram-negative anaerobe belonging to the gut microflora. It protects from experimental colitis induced by Helicobacter hepaticus through the polysaccharide A (PSA). The aim of our study was to establish: 1) whether B. fragilis colonization could protect from B. henselae infection; if this event may have beneficial effects on EPCs, vascular system and tissues. Our in vitro results establish for the first time that B. fragilis can internalize EPCs and competes with B. henselae during coinfection. We observed a marked activation of the inflammatory response by Real-time PCR and ELISA in coinfected cells compared to B. henselae-infected cells (63 vs 23 up-regulated genes), and after EPCs infection with mutant B. fragilis ΔPSA (≅90% up-regulated genes) compared to B. fragilis. Interestingly, in a mouse model of coinfection, morphological and ultrastructural analyses by hematoxylin-eosin staining and electron microscopy on murine tissues revealed that damages induced by B. henselae can be prevented in the coinfection with B. fragilis but not with its mutant B. fragilis ΔPSA. Moreover, immunohistochemistry analysis with anti-Bartonella showed that the number of positive cells per field decreased of at least 50% in the liver (20±4 vs 50±8), aorta (5±1 vs 10±2) and spleen (25±3 vs 40±6) sections of mice coinfected compared to mice infected only with B. henselae. This decrease was less evident in the coinfection with ΔPSA strain (35±6 in the liver, 5±1 in the aorta and 30±5 in the spleen). Finally, B. fragilis colonization was also able to restore the EPC decrease observed in mice infected with B. henselae (0.65 vs 0.06 media). Thus, our data establish that B. fragilis colonization is able to prevent B. henselae damages through PSA. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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38. Screening for GJB2 and GJB6 gene mutations in patients from Campania region with sensorineural hearing loss.
- Author
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Chinetti, Viviana, Iossa, Sandra, Auletta, Gennaro, Laria, Carla, de Luca, Maria, Di Leva, Francesca, Riccardi, Pasquale, Giannini, Pasquale, Gasparini, Paolo, Ciccodicola, Alfredo, Marciano, Elio, and Franzè, Annamaria
- Subjects
GENETIC mutation ,SENSORINEURAL hearing loss ,CONNEXINS ,HEARING - Abstract
Abstract The aim of this study was to screen 349 patients affected by sensorineural hearing loss (SNHL), mostly from the Campania region (southern Italy), for GJB2 gene mutations and for two deletions of the GJB6 gene (del GJB6 -D13S1830 and del GJB6 -D13S1854). We identified pathogenetic GJB2 mutations in 51 cases (15% of patients). No GJB6 mutation was found. We also examined the audiologic features of the patients for whom we had an etiologic diagnosis, in order to identify correlations between the severity of hearing loss and the type of mutation. Sumario El objetivo de este estudio fue evaluar 349 pacientes afecta-dos de una hipoacusia sensorineural (SNHL), sobre todo de la región de Campania (Italia del sur), buscando muta-ciones en el gen GJB2 y dos deleciones en el gen GJB6 (del GJB6-D13S1830 y del GJB6-13S1854). Identifica-mos mutaciones patogénicas en 51 pacientes (15% de los pacientes). No se encontraron mutaciones del gen GJB6. También examinamos los rasgos audiológicos de aquellos pacientes de quienes teníamos diagnóstico etiológico, para identificar correlaciones entre la severidad de la pérdida audi-tiva y el tipo de mutación. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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- View/download PDF
39. Uncovering the Complexity of Transcriptomes with RNA-Seq.
- Author
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Costa, Valerio, Angelini, Claudia, De Feis, Italia, and Ciccodicola, Alfredo
- Subjects
RNA ,MEDICINE ,BIOTECHNOLOGY ,DNA ,BIOINFORMATICS - Abstract
In recent years, the introduction of massively parallel sequencing platforms for Next Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred thousand DNA fragments, dramatically changed the landscape of the genetics studies. RNA-Seq for transcriptome studies, Chip-Seq for DNA-proteins interaction, CNV-Seq for large genome nucleotide variations are only some of the intriguing new applications supported by these innovative platforms. Among them RNA-Seq is perhaps the most complex NGS application. Expression levels of specific genes, differential splicing, allele-specific expression of transcripts can be accurately determined by RNA-Seq experiments to address many biological-related issues. All these attributes are not readily achievable from previously widespread hybridization-based or tag sequence-based approaches. However, the unprecedented level of sensitivity and the large amount of available data produced by NGS platforms provide clear advantages as well as new challenges and issues. This technology brings the great power to make several new biological observations and discoveries, it also requires a considerable effort in the development of new bioinformatics tools to deal with these massive data files. The paper aims to give a survey of the RNA-Seq methodology, particularly focusing on the challenges that this application presents both from a biological and a bioinformatics point of view. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
40. PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues.
- Author
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Costa, Valerio, Gallo, Maria Assunta, Letizia, Francesca, Aprile, Marianna, Casamassimi, Amelia, and Ciccodicola, Alfredo
- Subjects
GENE expression ,PEROXISOMES ,GENETIC regulation ,TRANSCRIPTION factors ,GENES - Abstract
Peroxisome proliferator-activated receptor gamma (PPARγ) is one of the most extensively studied ligand-inducible transcription factors (TFs), able to modulate its transcriptional activity through conformational changes. It is of particular interest because of its pleiotropic functions: it plays a crucial role in the expression of key genes involved in adipogenesis, lipid and glucid metabolism, atherosclerosis, inflammation, and cancer. Its protein isoforms, the wide number of PPARγ target genes, ligands, and coregulators contribute to determine the complexity of its function. In addition, the presence of genetic variants is likely to affect expression levels of target genes although the impact of PPARG gene variations on the expression of target genes is not fully understood. The introduction of massively parallel sequencing platforms--in the Next Generation Sequencing (NGS) era--has revolutionized the way of investigating the genetic causes of inherited diseases. In this context, DNA-Seq for identifying--within both coding and regulatory regions of PPARG gene--novel nucleotide variations and haplotypes associated to human diseases, ChIP-Seq for defining a PPARγ binding map, and RNA-Seq for unraveling the wide and intricate gene pathways regulated by PPARG, represent incredible steps toward the understanding of PPARγ in health and disease. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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- View/download PDF
41. TNFα Mediates Inflammation-Induced Effects on PPARG Splicing in Adipose Tissue and Mesenchymal Precursor Cells.
- Author
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Cataldi, Simona, Aprile, Marianna, Melillo, Daniela, Mucel, Inès, Giorgetti-Peraldi, Sophie, Cormont, Mireille, Italiani, Paola, Blüher, Matthias, Tanti, Jean-François, Ciccodicola, Alfredo, and Costa, Valerio
- Subjects
PI3K/AKT pathway ,INSULIN resistance ,PEOPLE with diabetes ,MACROPHAGES ,ADIPOSE tissues ,FAT cells ,OBESITY - Abstract
Low-grade chronic inflammation and reduced differentiation capacity are hallmarks of hypertrophic adipose tissue (AT) and key contributors of insulin resistance. We identified PPARGΔ5 as a dominant-negative splicing isoform overexpressed in the AT of obese/diabetic patients able to impair adipocyte differentiation and PPARγ activity in hypertrophic adipocytes. Herein, we investigate the impact of macrophage-secreted pro-inflammatory factors on PPARG splicing, focusing on PPARGΔ5. We report that the epididymal AT of LPS-treated mice displays increased PpargΔ5/cPparg ratio and reduced expression of Pparg-regulated genes. Interestingly, pro-inflammatory factors secreted from murine and human pro-inflammatory macrophages enhance the PPARGΔ5/cPPARG ratio in exposed adipogenic precursors. TNFα is identified herein as factor able to alter PPARG splicing—increasing PPARGΔ5/cPPARG ratio—through PI3K/Akt signaling and SRp40 splicing factor. In line with in vitro data, TNFA expression is higher in the SAT of obese (vs. lean) patients and positively correlates with PPARGΔ5 levels. In conclusion, our results indicate that inflammatory factors secreted by metabolically-activated macrophages are potent stimuli that modulate the expression and splicing of PPARG. The resulting imbalance between canonical and dominant negative isoforms may crucially contribute to impair PPARγ activity in hypertrophic AT, exacerbating the defective adipogenic capacity of precursor cells. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
42. DDXIIL: a novel transcript family emerging from human subtelomeric regions.
- Author
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Costa, Valerio, Casamassimi, Amelia, Roberto, Roberta, Gianfrancesco, Fernando, Matarazzo, Maria R., D'Urso, Michele, D'Esposito, Maurizio, Rocchi, Mariano, and Ciccodicola, Alfredo
- Subjects
HUMAN chromosomes ,MATERIAL plasticity ,HUMAN genome ,GENES ,X chromosome - Abstract
Background: The subtelomeric regions of human chromosomes exhibit an extraordinary plasticity. To date, due to the high GC content and to the presence of telomeric repeats, the subtelomeric sequences are underrepresented in the genomic libraries and consequently their sequences are incomplete in the finished human genome sequence, and still much remains to be learned about subtelomere organization, evolution and function. Indeed, only in recent years, several studies have disclosed, within human subtelomeres, novel gene family members. Results: During a project aimed to analyze genes located in the telomeric region of the long arm of the human X chromosome, we have identified a novel transcript family, DDX11L, members of which map to 1pter, 2q13/14.1, 2qter, 3qter, 6pter, 9pter/9qter, 11pter, 12pter, 15qter, 16pter, 17pter, 19pter, 20pter/20qter, Xpter/Xqter and Yqter. Furthermore, we partially sequenced the underrepresented subtelomeres of human chromosomes showing a common evolutionary origin. Conclusion: Our data indicate that an ancestral gene, originated as a rearranged portion of the primate DDX11 gene, and propagated along many subtelomeric locations, is emerging within subtelomeres of human chromosomes, defining a novel gene family. These findings support the possibility that the high plasticity of these regions, sites of DNA exchange among different chromosomes, could trigger the emergence of new genes. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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43. Identification of a novel mutation in the myosin VIIA motor domain in a family with autosomal dominant hearing loss (DFNA11).
- Author
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Di Leva, Francesca, D'Adamo, Pio, Cubellis, Maria Vittoria, D'Eustacchio, Angela, Errichiello, Monica, Saulino, Claudio, Auletta, Gennaro, Giannini, Pasquale, Donaudy, Francesca, Ciccodicola, Alfredo, Gasparini, Paolo, Franzè, Annamaria, Marciano, Elio, and Franzè, Annamaria
- Subjects
EAR diseases ,HEARING disorders ,VESTIBULAR apparatus diseases ,MYOSIN - Abstract
We ascertained a large Italian family with an autosomal dominant form of non-syndromic sensorineural hearing loss with vestibular involvement. A genome-wide scan found linkage to locus DFNA11. Sequencing of the MYO7A gene in the linked region identified a new missense mutation resulting in an Ala230Val change in the motor domain of the myosin VIIA. Myosin VIIA has already been implicated in several forms of deafness, but this is the third mutation causing a dominant form of deafness, located in the myosin VIIA motor domain in a region never involved in hearing loss until now. A modelled protein structure of myosin VII motor domain provides evidence for a significant functional effect of this missense mutation. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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- View/download PDF
44. Identification and characterisation of the retinitis pigmentosa 1-like1 gene (RP1L1): a novel candidate for retinal degenerations.
- Author
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Conte, Ivan, Lestingi, Marta, den Hollander, Anneke, Alfano, Giovanna, Ziviello, Carmela, Pugliese, Mariarosaria, Circolo, Diego, Caccioppoli, Cristina, Ciccodicola, Alfredo, and Banfi, Sandro
- Subjects
RETINITIS pigmentosa ,RETINAL diseases ,HUMAN genetics - Abstract
Retinitis pigmentosa (RP) is the most common form of inherited retinopathy, with an approximate incidence of 1 in 3700 individuals worldwide. Mutations in the retinitis pigmentosa 1 (RP1) gene are responsible for about 5-10% cases of autosomal dominant RP. The RP1 gene is specifically expressed in the photoreceptor layers of the postnatal retina and encodes a predicted protein characterised by the presence of two doublecortin (DC) domains, known to be implicated in microtubule binding. We identified and characterised, both in human and in mouse, a novel mammalian gene, termed Retinitis Pigmentosa1-like1 (RP1L1), because of its significant sequence similarity to the RP1 gene product. The sequence homology between RP1 and RP1L1 was found to be mostly restricted to the DC domains and to the N-terminal region, including the first 350 amino acids. The RP1L1 gene was also found to be conserved in distant vertebrates, since we identified a homologue in Fugu rubripes (pufferfish). Similar to RP1, RP1L1 expression is restricted to the postnatal retina, as determined by semiquantitative reverse transcriptase-PCR and Northern analysis. The retina-specific expression and the sequence similarity to RP1 render RPIL1 a potential candidate for inherited retinal disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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45. Multiple pathogenic and benigngenomic rearrangements occur at a 35 kb duplication involving the NEMOandLAGE2 genes.
- Author
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Aradhya, Swaroop, Bardaro, Tiziana, Galgóczy, Petra, Esposito, Teresa, Patlan, Henry, Ciccodicola, Alfredo, Munnich, Arnold, Kenwrick, Sue, Platzer, Matthias, D’Urso, Michele, and Nelson, David L.
- Published
- 2001
- Full Text
- View/download PDF
46. Identification of novel RP2 mutations in a subset of X-linked retinitis pigmentosa families and prediction of new domains.
- Author
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Miano, Maria Giuseppina, Testa, Francesco, Filippini, Francesco, Trujillo, Mariajosè, Conte, Ivan, Lanzara, Carmela, Millán, Josè Maria, De Bernardo, Carmelilia, Grammatico, Barbara, Mangino, Massimo, Torrente, Isabella, Carrozzo, Romeo, Simonelli, Francesca, Rinaldi, Ernesto, Ventruto, Valerio, D'Urso, Michele, Ayuso, Carmen, and Ciccodicola, Alfredo
- Published
- 2001
- Full Text
- View/download PDF
47. Mutational hot spot within a new RPGR exon in X-linked retinitis pigmentosa.
- Author
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Vervoort, Raf, Lennon, Alan, Bird, Alan C., Tulloch, Brian, Axton, Richard, Miano, Maria G., Meindl, Alfons, Meitinger, Thomas, Ciccodicola, Alfredo, and Wright, Alan F.
- Subjects
RETINITIS pigmentosa ,EXONS (Genetics) ,GENETIC mutation - Abstract
The gene RPGR was previously identified in the RP3 region of Xp21.1 and shown to be mutated in 10-20% of patients with the progressive retinal degeneration X-linked retinitis pigmentosa (XLRP). The mutations predominantly affected a domain homologous to RCC1, a guanine nucleotide exchange factor for the small GTPase Ran, although they were present in fewer than the 70-75% of XLRP patients predicted from linkage studies. Mutations in the RP2 locus at Xp11.3 were found in a further 10-20% of XLRP patients, as predicted from linkage studies. Because the mutations in the remainder of the XLRP patients may reside in undiscovered exons of RPGR, we sequenced a 172kb region containing the entire gene. Analysis of the sequence disclosed a new 3' terminal exon that was mutated in 60% of XLRP patients examined. This exon encodes 567 amino acids, with a repetitive domain rich in glutamic acid residues. The sequence is conserved in the mouse, bovine and Fugu rubripes genes. It is preferentially expressed in mouse and bovine retina, further supporting its importance for retinal function. Our results suggest that mutations in RPGR are the only cause of RP3 type XLRP and account for the disease in over 70% of XLRP patients and an estimated 11% of all retinitis pigmentosa patients. [ABSTRACT FROM AUTHOR]
- Published
- 2000
- Full Text
- View/download PDF
48. Mutation analysis of the RPGR gene reveals novel mutations in south European patients with X-linked retinitis pigmentosa.
- Author
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Miano, Maria Giuseppina, Testa, Francesco, Strazzullo, Maria, Trujillo, Mariajose, De Bernardo, Carmelilia, Grammatico, Barbara, Simonelli, Francesca, Mangino, Massimo, Torrente, Isabella, Ruberto, Giulio, Beneyto, Magdalena, Antinolo, Guillermo, Rinaldi, Ernesto, Danesino, Cesare, Ventruto, Valerio, D'Urso, Michele, Ayuso, Carmen, Baiget, Monserrat, and Ciccodicola, Alfredo
- Subjects
GENETIC mutation ,RETINITIS pigmentosa ,GUANOSINE triphosphatase - Abstract
The RPGR (retinitis pigmentosa GTPase regulator) gene has been shown to be mutated in 10-20% of patients with X-linked retinitis pigmentosa (XLRP), a severe form of inherited progressive retinal degeneration. A total of 29 different RPGR mutations have been identified in northern European and United States patients. We have performed mutation analysis of the RPGR gene in a cohort of 49 southern European males affected with XLRP. By multiplex SSCA and automatic direct sequencing of all 19 RPGR exons, seven different and novel mutations were identified in eight of the 49 families; these include three splice site mutations, two microdeletions, and two missense mutations. RNA analysis showed that the three splice site defects resulted in the generation of aberrant RPGR transcripts. Six of these mutations were detected in the conserved amino-terminal region of RPGR protein, containing tandem repeats homologous to the RCC1 protein, a guanine nucleotide-exchange factor for RanGTPase. Several exonic and intronic sequence variations were also detected. None of the RPGR mutations reported in other populations were identified in our series. Our results are consistent with the notions of heterogeneity and minority causation of XLRP by mutations in RPGR in Caucasian populations. [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
49. Complete congenital stationary night blindness maps on Xp11.4 in a Sardinian family.
- Author
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Rozzo, Carla, Fossarello, Maurizio, Galleri, Grazia, Miano, Maria Giuseppina, Ciccodicola, Alfredo, Sole, Gabriella, and Pirastu, Mario
- Subjects
GENETICS of blindness - Abstract
X-linked congenital stationary night blindness (CSNBX) is a hereditary non-progressive retinal disorder, which can appear in two different clinical forms, complete and incomplete, associated with CSNB1 and CSNB2 loci on Xp. We describe a Sardinian family with complete CSNBX and define better the limits of the CSNB1 genetic locus on Xp11.4 through linkage analysis. Haplotype analysis showed two key recombinants, which restrict the CSNB1 locus to a region of about 3 cM limited by markers DSX1068 and DSX6810 respectively. The locus that we describe is included in the CSNB1 locus defined by previous reports referring to the same clinical form of the disease. These results, in addition to other recent mapping reports about families from different geographical areas, confirm the genetic homogeneity of X-linked complete CSNB. [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
50. A novel pseudoautosomal human gene encodes a putative protein similar to Ac-like transposases.
- Author
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Esposito, Teresa, Gianfrancesco, Fernando, Ciccodicola, Alfredo, Montanini, Luisa, Mumm, Steven, D, Michele, and Forabosco, Antonino
- Abstract
We report the cloning of a novel gene, called Tramp, in the Xp/Yp PAR region that has a functional homologue on the Y chromosome and escapes X-inactivation. This gene encodes, within a single exon, a putative protein that has amino acid similarity with transposases of the Ac family. Flanking this gene we have identified putative terminal inverted repeats (TIRs) and a duplicate target site, suggesting that it may be an ancient transposable element. The nucleotide differences in these sites and the TIR-binding inactivity of the putative Tramp protein suggest that this element is not an autonomous transposon. In the human genome, the Tramp protein may be involved in the transposition of other transposable elements, like medium reiterated frequency repeats, or it could be specialized in the acquisition of a new cellular function. [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
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