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International audience; The protocooperation between Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus relies on metabolite exchanges that accelerate acidification during yogurt fermentation. Conflicting results have been obtained in terms of the effect of the Strep. thermophilus urease and the NH3 and CO2 that it generates on the rate of acidification in yogurt fermentation. It is difficult to perform a systematic study of the effects of urease on protocooperation because it is necessary to distinguish among the direct, indirect, and strain-specific effects resulting from the combination of the strains of both species. To evaluate the direct effects of urease on protocooperation, we generated 3 urease-deficient mutants (Delta ureC) of fast- and slow-acidifying Strep. thermophilus strains and observed the effects of NH3 or CO2 supplementation on acidification by the Delta ureC strains. Further, we examined 5 combinations of 3 urease-deficient Delta ureC strains with 2 CO2-responsive or CO2 -unresponsive strains of L. bulgaricus. Urease deficiency induced a shortage of ammonia nitrogen and CO2 for the fast- and slow-acidifying Strep. thermophilus and for the CO2-responsive L. bulgaricus, respectively. Notably, the shortage of ammonia nitrogen had more severe effects than that of CO2 on yogurt fermentation, even if coculture with L. bulgaricus masked the effect of urease deficiency. Our work established (1) that urease deficiency inhibits the fermentative acceleration of protocooperation regardless of the Strep. thermophilus and L. bulgaricus strain combinations, and (2) that urease is an essential factor for effective yogurt acidification.

A modified version of an operational 3-dimensional, non-hydrostatic, limited-area atmospheric model (MM5) was used to perform high-resolution, idealized simulations of the interaction of an infinitely long single ridge with a steady, lateral large-scale wind field. The effect of different mountain ridge dimensions, wind speeds and patterns and moisture profiles on the quantity and distribution of orographic rainfall was investigated. The simulations demonstrated a number of commonly observed mountain flow features like formation of cap clouds, foehn wall, convective break-out associated with mountain topography, interaction of downslope winds with sea breeze, and different stages of cumulus development. It was found that the rainfall maxima associated with the mountain always occur upstream of the ridge peak. Changing mountain dimensions, wind speeds and patterns and moisture profile had clear effects on amount and pattern of accumulated rainfall. Low wind speeds resulted the maximum accumulated rainfall to occur considerable distance upstream of ridge peak. Reversal of wind directions in upper atmosphere caused rainfall to be largely restricted to the wind-side of the peak. The observed rainfall patterns are explained by the different flow patterns observed in the model output.

International audience; Lead halide perovskites are attracting a great deal of interest for optoelectronic applications such as solar cells, LEDs, and lasers because of their unique properties. In solar cells, heat dissipation by hot carriers results in a major energy loss channel responsible for the Shockley–Queisser efficiency limit. Hot carrier solar cells offer the possibility to overcome this limit and achieve energy conversion efficiency as high as 66% by extracting hot carriers. Therefore, fundamental studies on hot carrier relaxation dynamics in lead halide perovskites are important. Here, we elucidated the hot carrier cooling dynamics in all-inorganic cesium lead iodide (CsPbI3) perovskite using transient absorption spectroscopy. We observe that the hot carrier cooling rate in CsPbI3 decreases as the fluence of the pump light increases and the cooling is as slow as a few 10 ps when the photoexcited carrier density is 7 × 1018 cm−3, which is attributed to phonon bottleneck for high photoexcited carrier densities. Our findings suggest that CsPbI3 has a potential for hot carrier solar cell applications.