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Inter-annual variation in the feeding habits and food sources of Japanese sardine and mackerel at age-0 and age-1+ caught in the Kuroshio-Oyashio transition zone of the Western North Pacific were investigated based on analyses of bulk stable isotopes (δ13C, δ15N) and amino acid nitrogen isotopes (δ15NAA). Differences in δ13C and δ15N between Japanese sardine and mackerel were small for age-0, and inter-annual variation trends were similar, suggesting they depend on similar food sources in the same food web at this age. In contrast, inter-annual variation in δ13C and δ15N were significantly different between both species at age-1+, and both δ15N of phenylalanine (δ15NPhe: an indicator of nitrogen source) and trophic position estimated from δ15NAA (TPAA) were higher in mackerel, suggesting that the two species depend on distinct food webs as they age. Inter-annual variations in δ15NPhe were considered to have different causes for the two species; differences in food web structure due to the degree of southward intrusion of the Oyashio Current for Japanese sardine, compared to a shift in migration area and depth for mackerel. Furthermore, competition for food due to the recent increases in the population densities of both fishes appeared to be reflected in increased TPAA of mackerel. Although they are caught in the same region, the mechanism of variation in food sources differs because of differences in migration area, depth, and feeding habits. Differences in the feeding habits of Japanese sardine and mackerel may affect trophic status and spawning characteristics, potentially leading to different shifts in stock abundances.

Nitrogen (N) saturation of upland forests has been assumed to be a substantial N source downstream. However, removal processes of N, including assimilation and denitrification in the downstream area, have not been clarified. To evaluate the N removal processes, nitrate (NO3−) and organic N concentrations, as well as nitrogen isotope ratio (δ15N) and oxygen isotope ratio (δ18O) of NO3− were measured along three rivers of Tatara River Basin, Japan where upland forests have already been N-saturated. Geographic information system (GIS) based topographical analysis was also conducted to evaluate the land use as urban area in relation to topography. In two of the three rivers, NO3− concentrations did not increase from upstream to downstream, despite the potential non-point N sources of urban areas. In another river, NO3− concentrations rather decreased. The values of δ15N and δ18O of NO3− and organic N concentrations suggested the presence of denitrification and assimilation over N pollutants in the river whose watersheds have a lower percentage of urban area. The lower percentage of urban area could be explained by the lower topographic index. This study concluded that the NO3− leaching from upland N-saturated forests was substantially assimilated or denitrified in the downstream area.