1. Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice
- Author
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Mara eDierssen, Laura eFedrizzi, Rosa eGomez-Villafuertes, Maria eMartinez de Lagran, Alfonso eGutierrez-Adan, Ignaci eSahun, Belen ePintado, Juan Carlos Oliveros, Xose Manuel Dopazo, Paz eGonzalez, Marisa eBrini, Britt eMellstrom, Ernesto eCarafoli, and JOSE R. NARANJO
- Subjects
Cerebellum ,Transgenic mice ,Ca2+-dependent gene expression ,daDREAM ,Genome-wide analysis ,Midline 1 gene ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
DREAM (downstream regulatory element antagonist modulator) is a Ca2+-binding protein that binds DNA and represses transcription in a Ca2+-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca2+ homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a severe delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function.
- Published
- 2012
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