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Stem cell (SC) division, deployment, and differentiation are processes that contribute to corneal epithelial renewal. Until now studying the destiny of these cells in a living mammal has not been possible. However, the advent of inducible multicolor genetic tagging and powerful imaging technologies has rendered this achievable in the translucent and readily accessible murine cornea. K14CreERT2-Confetti mice that harbor two copies of the Brainbow 2.1 cassette, yielding up to 10 colors from the stochastic recombination of fluorescent proteins, were used to monitor K-14+ progenitor cell dynamics within the corneal epithelium in live animals. Multicolored columns of cells emerged from the basal limbal epithelium as they expanded and migrated linearly at a rate of 10.8 µm/day toward the central cornea. Moreover, the permanent expression of fluorophores, passed on from progenitor to progeny, assisted in discriminating individual clones as spectrally distinct streaks containing more than 1,000 cells within the illuminated area. The centripetal clonal expansion is suggestive that a single progenitor cell is responsible for maintaining a narrow corridor of corneal epithelial cells. Our data are in agreement with the limbus as the repository for SC as opposed to SC being distributed throughout the central cornea. This is the first report describing stem/progenitor cell fate determination in the murine cornea using multicolor genetic tracing. This model represents a powerful new resource to monitor SC kinetics and fate choice under homeostatic conditions, and may assist in assessing clonal evolution during corneal development, aging, wound-healing, disease, and following transplantation. S tem C ells 2015;33:157-169 [ABSTRACT FROM AUTHOR]

The Life Cycle Assessment (LCA) of biogas-fed Solid Oxide Fuel Cell (SOFC) integrated with a CO 2 recovery system is presented in this work. The goal of the work is to evaluate the environmental performance of an SOFC fueled with sewage biogas and to compare it with traditional technologies (internal combustion engines and microturbines) using the same fuel. CO 2 recovery is performed through a tubular photobioreactor, fixing the recovered carbon in the form of a micro-algae. The analysis takes into account both the biogas production line (anaerobic digester) and its exploitation into the fuel cell (i.e., the power generator). Results show that the SOFC manufacturing activity is highly intensive since it requires a large amount of use of electricity. During operation, instead, the highest burden is associated with the fuel production. We analyzed two scenarios for biogas operation underlining the benefits of introducing sludge pre-thickening before the anaerobic digestion process. The use of a sludge pre-thickening system can reduce the inlet flow of natural gas into the plant, thus affecting the fuel chain contribution and reducing the overall impact. The photobioreactor results in consuming more energy than what it produces (looking at the operation phase only; the manufacturing phase was not even included) and being responsible for more carbon emissions than the amount fixed in algae. On the other side, data for the photobioreactor system were based on a real system at the proof-of-concept level. Therefore significant improvements are expected for an industrial-size system. Finally, the SOFC environmental burdens have been compared with main competitors in the same field (internal combustion engines and microturbines), showing the superior environmental performance. The proposed energy system is thus an interesting choice for cleaner energy production. [ABSTRACT FROM AUTHOR]