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Objective To investigate the expression and localization of Aurora kinase A (AURKA) and Aurora kinase B (AURKB) in mouse zygotes during the process of the first mitosis. Methods Quantitative real-time RT-PCR and Western blotting were performed to analyze the expression of AURKA and AURKB. The subcellular location of AURKA and AURKB was studied by confocal microscopy. Results AURKA and AURKB were increasingly expressed from phase G 1 and peaked at phase M. After the entrance into mitosis AURKB became the predominant form both in mRNA and protein levels. The proteins of AURKA and AURKB both distributed in the cytoplasm and were associated with nucleus during the first mitosis of mouse zygotes, with some details in different. Conclusion The expression and localization of Aurora kinases A and B was in a cellcycle regulated manner during the process of the cleavage of mouse zygotes. This discovery will aid in future investigations on their specific roles and molecular mechanisms in the regulation of mammalian early embryonic development.

Objective To investigate the biology function of novel protein related to bovine seminal plasma protein in human testis. Methods Recombination pcDNA3/HBRP was constructed and transfected to HEK293 cell and permanently expression cell line was established. The activity of protein kinase C (PKC) of the cell line was detected by autoradiography method. Results The stable expression cell line of HBRP was obtained. The HBRP inhibited the activity of PKC significantly. Conclusion One of the new functions of novel sperm binding protein in human is the inhibitor action on activity of PKC. It may be involved in the sperm capacitation and acrosome reaction.