43 results on '"Barbitoff, Yury A."'
Search Results
2. Major Causes of Conflicting Interpretations of Variant Pathogenicity in Rare Disease: A Systematic Analysis.
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Lazareva, Tatyana E., Barbitoff, Yury A., Nasykhova, Yulia A., and Glotov, Andrey S.
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HUMAN genetic variation , *GENETIC variation , *MUSCLE growth , *NUCLEOTIDE sequencing , *RARE diseases - Abstract
The identification of the genetic causes of inherited disorders from next-generation sequencing (NGS) data remains a complicated process, in particular due to challenges in interpretation of the vast amount of generated data and hundreds of candidate variants identified. Inconsistencies in variant classification, where genetic centers classify the same variant differently, can hinder accurate diagnoses for rare diseases. Publicly available databases that collect data on human genetic variations and their association with diseases provide ample opportunities to discover conflicts in variant interpretation worldwide. In this study, we explored patterns of variant classification discrepancies using data from ClinVar, a public archive of variant interpretations. We found that 5.7% of variants have conflicting interpretations (COIs) reported, and the vast majority of interpretation conflicts arise for variants of uncertain significance (VUS). As many as 78% of clinically relevant genes harbor variants with COIs, and genes with high COI rates tended to have more exons and longer transcripts, with a greater proportion of genes linked to several distinct conditions. The enrichment analysis of COI-enriched genes revealed that the products of these genes are involved in cardiac disorders, muscle development, and function. To improve diagnoses, we believe that specific variant interpretation rules could be developed for such genes. Additionally, our findings underscore the need for the publication of variant pathogenicity evidence and the importance of considering every variant as VUS unless proven otherwise. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Replication of Known and Identification of Novel Associations in Biobank-Scale Datasets: A Survey Using UK Biobank and FinnGen.
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Tkachenko, Alexander A., Changalidis, Anton I., Maksiutenko, Evgeniia M., Nasykhova, Yulia A., Barbitoff, Yury A., and Glotov, Andrey S.
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GENOME-wide association studies - Abstract
Over the last two decades, numerous genome-wide association studies (GWAS) have been performed to unveil the genetic architecture of human complex traits. Despite multiple efforts aimed at the trans-biobank integration of GWAS results, no systematic analysis of the variant-level properties affecting the replication of known associations (or identifying novel ones) in genome-wide meta-analysis has yet been performed using biobank-scale data. To address this issue, we performed a systematic comparison of GWAS summary statistics for 679 complex traits in the UK Biobank (UKB) and FinnGen (FG) cohorts. We identified 37,148 index variants with genome-wide associations with at least one trait in either cohort or in the meta-analysis, only 3528 (9.5%) of which were shared between UKB and FG. Nearly twice as many variants (6577) were replicated in another dataset at the significance level adjusted for the number of variants selected for replication. However, as many as 9230 loci failed to be replicated. Moreover, as many as 5813 loci were observed as significant associations only in meta-analysis results, highlighting the importance of trans-biobank meta-analysis efforts. We showed that variants that failed to replicate in UKB or FG tend to correspond to rare, less pleiotropic variants with lower effect sizes and lower LD score values. Genome-wide associations specific to meta-analysis were also enriched in low-effect variants; however, such variants tended to be more common and have more consistent frequencies between populations. Taken together, our results show a relatively high rate of non-replication of genome-wide associations in the studied cohorts and highlight both widely appreciated and less acknowledged properties of the associations affecting their identification and replication. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Gene Expression Analysis of Yeast Strains with a Nonsense Mutation in the eRF3-Coding Gene Highlights Possible Mechanisms of Adaptation.
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Maksiutenko, Evgeniia M., Barbitoff, Yury A., Danilov, Lavrentii G., Matveenko, Andrew G., Zemlyanko, Olga M., Efremova, Elena P., Moskalenko, Svetlana E., and Zhouravleva, Galina A.
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GENE expression , *NONSENSE mutation , *PROTEOMICS , *TRANSCRIPTION factors , *GENETIC mutation , *CELL cycle regulation - Abstract
In yeast Saccharomyces cerevisiae, there are two translation termination factors, eRF1 (Sup45) and eRF3 (Sup35), which are essential for viability. Previous studies have revealed that presence of nonsense mutations in these genes leads to amplification of mutant alleles (sup35-n and sup45-n), which appears to be necessary for the viability of such cells. However, the mechanism of this phenomenon remained unclear. In this study, we used RNA-Seq and proteome analysis to reveal the complete set of gene expression changes that occur during cellular adaptation to the introduction of the sup35-218 nonsense allele. Our analysis demonstrated significant changes in the transcription of genes that control the cell cycle: decreases in the expression of genes of the anaphase promoting complex APC/C (APC9, CDC23) and their activator CDC20, and increases in the expression of the transcription factor FKH1, the main cell cycle kinase CDC28, and cyclins that induce DNA biosynthesis. We propose a model according to which yeast adaptation to nonsense mutations in the translation termination factor genes occurs as a result of a delayed cell cycle progression beyond the G2-M stage, which leads to an extension of the S and G2 phases and an increase in the number of copies of the mutant sup35-n allele. [ABSTRACT FROM AUTHOR]
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- 2024
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5. The spectrum of pathogenic variants of the ATP7B gene in Wilson disease in the Russian Federation
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Balashova, Mariya S., Tuluzanovskaya, Inna G., Glotov, Oleg S., Glotov, Andrey S., Barbitoff, Yury A., Fedyakov, Mikhail A., Alaverdian, Diana A., Ivashchenko, Tatiana E., Romanova, Olga V., Sarana, Andrey M., Scherbak, Sergey G., Baranov, Vladislav S., Filimonov, Marat I., Skalny, Anatoly V., Zhuchenko, Natalya A., Ignatova, Tatiana M., and Asanov, Aliy Y.
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- 2020
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6. Exome sequencing in extreme altitude mountaineers identifies pathogenic variants in RTEL1 and COL6A1 previously associated with respiratory failure.
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Maksiutenko, Evgeniia M., Merkureva, Valeriia, Barbitoff, Yury A., Tsay, Victoria V., Aseev, Mikhail V., Glotov, Andrey S., and Glotov, Oleg S.
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RESPIRATORY insufficiency ,ALTITUDES ,MOUNTAINEERING ,PULMONARY fibrosis ,MOUNTAINEERS - Abstract
Adaptation of humans to challenging environmental conditions, such as extreme temperature, malnutrition, or hypoxia, is an interesting phenomenon for both basic and applied research. Identification of the genetic factors contributing to human adaptation to these conditions enhances our understanding of the underlying molecular and physiological mechanisms. In our study, we analyzed the exomes of 22 high altitude mountaineers to uncover genetic variants contributing to hypoxic adaptation. To our surprise, we identified two putative loss‐of‐function variants, rs1385101139 in RTEL1 and rs1002726737 in COL6A1 in two extremely high altitude (personal record of more than 8500 m) professional climbers. Both variants can be interpreted as pathogenic according to medical geneticists' guidelines, and are linked to inherited conditions involving respiratory failure (late‐onset pulmonary fibrosis and severe Ullrich muscular dystrophy for rs1385101139 and rs1002726737, respectively). Our results suggest that a loss of gene function may act as an important factor of human adaptation, which is corroborated by previous reports in other human subjects. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Bioinformatics of germline variant discovery for rare disease diagnostics: current approaches and remaining challenges.
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Barbitoff, Yury A, Ushakov, Mikhail O, Lazareva, Tatyana E, Nasykhova, Yulia A, Glotov, Andrey S, and Predeus, Alexander V
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RARE diseases , *GENETIC variation , *WHOLE genome sequencing , *HUMAN genome , *MEDICAL genomics , *NUCLEOTIDE sequencing , *MEDICAL genetics - Abstract
Next-generation sequencing (NGS) has revolutionized the field of rare disease diagnostics. Whole exome and whole genome sequencing are now routinely used for diagnostic purposes; however, the overall diagnosis rate remains lower than expected. In this work, we review current approaches used for calling and interpretation of germline genetic variants in the human genome, and discuss the most important challenges that persist in the bioinformatic analysis of NGS data in medical genetics. We describe and attempt to quantitatively assess the remaining problems, such as the quality of the reference genome sequence, reproducible coverage biases, or variant calling accuracy in complex regions of the genome. We also discuss the prospects of switching to the complete human genome assembly or the human pan-genome and important caveats associated with such a switch. We touch on arguably the hardest problem of NGS data analysis for medical genomics, namely, the annotation of genetic variants and their subsequent interpretation. We highlight the most challenging aspects of annotation and prioritization of both coding and non-coding variants. Finally, we demonstrate the persistent prevalence of pathogenic variants in the coding genome, and outline research directions that may enhance the efficiency of NGS-based disease diagnostics. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Systematic dissection of biases in whole-exome and whole-genome sequencing reveals major determinants of coding sequence coverage
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Barbitoff, Yury A., Polev, Dmitrii E., Glotov, Andrey S., Serebryakova, Elena A., Shcherbakova, Irina V., Kiselev, Artem M., Kostareva, Anna A., Glotov, Oleg S., and Predeus, Alexander V.
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- 2020
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9. Phenome-wide functional dissection of pleiotropic effects highlights key molecular pathways for human complex traits
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Shikov, Anton E., Skitchenko, Rostislav K., Predeus, Alexander V., and Barbitoff, Yury A.
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- 2020
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10. Russian Regional Differences in Allele Frequencies of CFTR Gene Variants: Genetic Monitoring of Infertile Couples.
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Glotov, Andrey S., Chernykh, Vyacheslav B., Solovova, Olga A., Polyakov, Aleksander V., Donnikov, Maksim Yu., Kovalenko, Ludmila V., Barbitoff, Yury A., Nasykhova, Yulia A., Lazareva, Tatyana E., and Glotov, Oleg S.
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GENETIC variation ,GENE frequency ,REGIONAL differences ,SEMEN analysis ,MALE infertility - Abstract
A male factor, commonly associated with poor semen quality, is revealed in about 50% of infertile couples. CFTR gene (Cystic Fibrosis Transmembrane Conduction Regulator) variants are one of the common genetic causes of azoospermia-related male infertility. Notably, the spectrum and frequency of pathogenic CFTR variants vary between populations and geographical regions. In this work, we made an attempt to evaluate the allele frequency (AF) of 12 common CFTR variants in infertile Russian men and healthy individuals from different districts of Russia. Because of the limited number of population-based studies on Russian individuals, we characterized the population AFs based on data from the Registry of Russian cystic fibrosis (CF) patients. In addition to the CF patient registry, we estimated the local frequencies of the same set of variants based on the results of genotyping of CF patients in local biocollections (from St. Petersburg and Yugra regions). AFs of common CFTR variants calculated based on registry and biocollection data showed good concordance with directly measured population AFs. The estimated region-specific frequencies of CFTR variants allowed us to uncover statistically significant regional differences in the frequencies of the F508del (c.1521_1523del; p.Phe508del) and CFTRdele2,3(21kb) (c.54-5940_273+10250del21kb; p.Ser18ArgfsX) variants. The data from population-based studies confirmed previous observations that F508del, CFTRdele2,3(21kb), and L138ins (c.413_415dup; p.Leu138dup)variants are the most abundant among infertile patients, and their frequencies are significantly lower in healthy individuals and should be taken into account during genetic monitoring of the reproductive health of Russian individuals. [ABSTRACT FROM AUTHOR]
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- 2024
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11. bioGWAS: A Simple and Flexible Tool for Simulating GWAS Datasets.
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Changalidis, Anton I., Alexeev, Dmitry A., Nasykhova, Yulia A., Glotov, Andrey S., and Barbitoff, Yury A.
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GENOME-wide association studies ,GENETIC variation ,BIOINFORMATICS software - Abstract
Simple Summary: Genome-wide association studies (GWAS) are a powerful tool for the identification of genes affecting human traits. Still, the interpretation of GWAS results is complicated, and new tools are actively being developed. Due to the scarcity of available datasets, simulation of GWAS data with known genetic effects is important as it enables accurate evaluation of such tools. In this study, we developed a flexible tool, bioGWAS, that provides a set of important functionalities for simulating GWAS results. We demonstrate that bioGWAS can efficiently generate GWAS results with predefined causal genes and biological processes and is capable of recapitulating the results of published GWAS studies. We thus believe that bioGWAS is an excellent method for testing bioinformatics software for GWAS results processing, as well as for the generation of datasets for educational purposes. Genome-wide association studies (GWAS) have proven to be a powerful tool for the identification of genetic susceptibility loci affecting human complex traits. In addition to pinpointing individual genes involved in a particular trait, GWAS results can be used to discover relevant biological processes for these traits. The development of new tools for extracting such information from GWAS results requires large-scale datasets with known biological ground truth. Simulation of GWAS results is a powerful method that may provide such datasets and facilitate the development of new methods. In this work, we developed bioGWAS, a simple and flexible pipeline for the simulation of genotypes, phenotypes, and GWAS summary statistics. Unlike existing methods, bioGWAS can be used to generate GWAS results for simulated quantitative and binary traits with a predefined set of causal genetic variants and/or molecular pathways. We demonstrate that the proposed method can recapitulate complete GWAS datasets using a set of reported genome-wide associations. We also used our method to benchmark several tools for gene set enrichment analysis for GWAS data. Taken together, our results suggest that bioGWAS provides an important set of functionalities that would aid the development of new methods for downstream processing of GWAS results. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Altered Sphingolipid Hydrolase Activities and Alpha-Synuclein Level in Late-Onset Schizophrenia.
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Usenko, Tatiana, Bezrukova, Anastasia, Basharova, Katerina, Baydakova, Galina, Shagimardanova, Elena, Blatt, Nataliya, Rizvanov, Albert, Limankin, Oleg, Novitskiy, Maxim, Shnayder, Natalia, Izyumchenko, Artem, Nikolaev, Mikhail, Zabotina, Anna, Lavrinova, Anna, Kulabukhova, Darya, Nasyrova, Regina, Palchikova, Ekaterina, Zalutskaya, Natalia, Miliukhina, Irina, and Barbitoff, Yury
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ALPHA-synuclein ,LYSOSOMES ,PARKINSON'S disease ,ENZYME-linked immunosorbent assay ,LYSOSOMAL storage diseases ,SCHIZOPHRENIA - Abstract
Recent data described that patients with lysosomal storage disorders (LSDs) may have clinical schizophrenia (SCZ) features. Disruption of lipid metabolism in SCZ pathogenesis was found. Clinical features of schizophrenia (SCZ) have been demonstrated in patients with several lysosomal storage disorders (LSDs). Taking into account the critical role of lysosomal function for neuronal cells' lysosomal dysfunction could be proposed in SCZ pathogenesis. The current study analyzed lysosomal enzyme activities and the alpha-synuclein level in the blood of patients with late-onset SCZ. In total, 52 SCZ patients with late-onset SCZ, 180 sporadic Parkinson's disease (sPD) patients, and 176 controls were recruited. The enzymatic activity of enzymes associated with mucopolysaccharidosis (alpha-L-Iduronidase (IDUA)), glycogenosis (acid alpha-glucosidase (GAA)) and sphingolipidosis (galactosylceramidase (GALC), glucocerebrosidase (GCase), alpha-galactosidase (GLA), acid sphingomyelinase (ASMase)) and concentration of lysosphingolipids (hexosylsphingosine (HexSph), globotriaosylsphingosine (LysoGb3), and lysosphingomyelin (LysoSM)) were measured using LC-MS/MS. The alpha-synuclein level was estimated in magnetically separated CD45+ blood cells using the enzyme-linked immunosorbent assay (ELISA). Additionally, NGS analysis of 11 LSDs genes was conducted in 21 early-onset SCZ patients and 23 controls using the gene panel PGRNseq-NDD. Decreased ASMase, increased GLA activities, and increased HexSpn, LysoGb3, and LysoSM concentrations along with an accumulation of the alpha-synuclein level were observed in late-onset SCZ patients in comparison to the controls (p < 0.05). Four rare deleterious variants among LSDs genes causing mucopolysaccharidosis type I (IDUA (rs532731688, rs74385837) and type III (HGSNAT (rs766835582)) and sphingolipidosis (metachromatic leukodystrophy (ARSA (rs201251634)) were identified in five patients from the group of early-onset SCZ patients but not in the controls. Our findings supported the role of sphingolipid metabolism in SCZ pathogenesis. Aberrant enzyme activities and compounds of sphingolipids associated with ceramide metabolism may lead to accumulation of alpha-synuclein and may be critical in SCZ pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2024
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13. The Landscape of Point Mutations in Human Protein Coding Genes Leading to Pregnancy Loss.
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Maksiutenko, Evgeniia M., Barbitoff, Yury A., Nasykhova, Yulia A., Pachuliia, Olga V., Lazareva, Tatyana E., Bespalova, Olesya N., and Glotov, Andrey S.
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MISCARRIAGE , *RECURRENT miscarriage , *PREGNANCY complications , *GENETIC code , *MOLECULAR genetics , *GENETIC variation - Abstract
Pregnancy loss is the most frequent complication of a pregnancy which is devastating for affected families and poses a significant challenge for the health care system. Genetic factors are known to play an important role in the etiology of pregnancy loss; however, despite advances in diagnostics, the causes remain unexplained in more than 30% of cases. In this review, we aggregated the results of the decade-long studies into the genetic risk factors of pregnancy loss (including miscarriage, termination for fetal abnormality, and recurrent pregnancy loss) in euploid pregnancies, focusing on the spectrum of point mutations associated with these conditions. We reviewed the evolution of molecular genetics methods used for the genetic research into causes of pregnancy loss, and collected information about 270 individual genetic variants in 196 unique genes reported as genetic cause of pregnancy loss. Among these, variants in 18 genes have been reported by multiple studies, and two or more variants were reported as causing pregnancy loss for 57 genes. Further analysis of the properties of all known pregnancy loss genes showed that they correspond to broadly expressed, highly evolutionary conserved genes involved in crucial cell differentiation and developmental processes and related signaling pathways. Given the features of known genes, we made an effort to construct a list of candidate genes, variants in which may be expected to contribute to pregnancy loss. We believe that our results may be useful for prediction of pregnancy loss risk in couples, as well as for further investigation and revealing genetic etiology of pregnancy loss. [ABSTRACT FROM AUTHOR]
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- 2023
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14. Plasma microRNA Profiling in Type 2 Diabetes Mellitus: A Pilot Study.
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Tonyan, Ziravard N., Barbitoff, Yury A., Nasykhova, Yulia A., Danilova, Maria M., Kozyulina, Polina Y., Mikhailova, Anastasiia A., Bulgakova, Olga L., Vlasova, Margarita E., Golovkin, Nikita V., and Glotov, Andrey S.
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TYPE 2 diabetes , *GENE expression , *NUCLEOTIDE sequencing , *MICRORNA , *METABOLIC disorders , *WEIGHT loss - Abstract
Type 2 diabetes mellitus (T2D) is a chronic metabolic disease characterized by insulin resistance and β-cell dysfunction and leading to many micro- and macrovascular complications. In this study we analyzed the circulating miRNA expression profiles in plasma samples from 44 patients with T2D and 22 healthy individuals using next generation sequencing and detected 229 differentially expressed miRNAs. An increased level of miR-5588-5p, miR-125b-2-3p, miR-1284, and a reduced level of miR-496 in T2D patients was verified. We also compared the expression landscapes in the same group of patients depending on body mass index and identified differential expression of miR-144-3p and miR-99a-5p in obese individuals. Identification and functional analysis of putative target genes was performed for miR-5588-5p, miR-125b-2-3p, miR-1284, and miR-496, showing chromatin modifying enzymes and apoptotic genes being among the significantly enriched pathways. [ABSTRACT FROM AUTHOR]
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- 2023
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15. Statistical Dissection of the Genetic Determinants of Phenotypic Heterogeneity in Genes with Multiple Associated Rare Diseases.
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Lazareva, Tatyana E., Barbitoff, Yury A., Nasykhova, Yulia A., Pavlova, Nadezhda S., Bogaychuk, Polina M., and Glotov, Andrey S.
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RARE diseases , *PHENOTYPES , *DISEASE complications , *GENETIC variation , *HETEROGENEITY - Abstract
Phenotypicheterogeneity is a phenomenon in which distinct phenotypes can develop in individuals bearing pathogenic variants in the same gene. Genetic factors, gene interactions, and environmental factors are usually considered the key mechanisms of this phenomenon. Phenotypic heterogeneity may impact the prognosis of the disease severity and symptoms. In our work, we used publicly available data on the association between genetic variants and Mendelian disease to investigate the genetic factors (such as the intragenic localization and type of a variant) driving the heterogeneity of gene–disease relationships. First, we showed that genes linked to multiple rare diseases (GMDs) are more constrained and tend to encode more transcripts with high levels of expression across tissues. Next, we assessed the role of variant localization and variant types in specifying the exact phenotype for GMD variants. We discovered that none of these factors is sufficient to explain the phenomenon of such heterogeneous gene–disease relationships. In total, we identified only 38 genes with a weak trend towards significant differences in variant localization and 30 genes with nominal significant differences in variant type for the two associated disorders. Remarkably, four of these genes showed significant differences in both tests. At the same time, our analysis suggests that variant localization and type are more important for genes linked to autosomal dominant disease. Taken together, our results emphasize the gene-level factors dissecting distinct Mendelian diseases linked to one common gene based on open-access genetic data and highlight the importance of exploring other factors that contributed to phenotypic heterogeneity. [ABSTRACT FROM AUTHOR]
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- 2023
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16. Current Status and Prospects of the Single-Cell Sequencing Technologies for Revealing the Pathogenesis of Pregnancy-Associated Disorders.
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Naydenov, Dmitry D., Vashukova, Elena S., Barbitoff, Yury A., Nasykhova, Yulia A., and Glotov, Andrey S.
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RECURRENT miscarriage ,PREMATURE labor ,POLYCYSTIC ovary syndrome ,GENE expression profiling ,RNA sequencing ,HYPERGLYCEMIA ,PREGNANCY complications - Abstract
Single-cell RNA sequencing (scRNA-seq) is a method that focuses on the analysis of gene expression profile in individual cells. This method has been successfully applied to answer the challenging questions of the pathogenesis of multifactorial diseases and open up new possibilities in the prognosis and prevention of reproductive diseases. In this article, we have reviewed the application of scRNA-seq to the analysis of the various cell types and their gene expression changes in normal pregnancy and pregnancy complications. The main principle, advantages, and limitations of single-cell technologies and data analysis methods are described. We discuss the possibilities of using the scRNA-seq method for solving the fundamental and applied tasks related to various pregnancy-associated disorders. Finally, we provide an overview of the scRNA-seq findings for the common pregnancy-associated conditions, such as hyperglycemia in pregnancy, recurrent pregnancy loss, preterm labor, polycystic ovary syndrome, and pre-eclampsia. [ABSTRACT FROM AUTHOR]
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- 2023
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17. Annotation of uORFs in the OMIM genes allows to reveal pathogenic variants in 5′UTRs.
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Filatova, Alexandra, Reveguk, Ivan, Piatkova, Maria, Bessonova, Daria, Kuziakova, Olga, Demakova, Victoria, Romanishin, Alexander, Fishman, Veniamin, Imanmalik, Yerzhan, Chekanov, Nikolay, Skitchenko, Rostislav, Barbitoff, Yury, Kardymon, Olga, and Skoblov, Mikhail
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- 2023
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18. Biobanking as a Tool for Genomic Research: From Allele Frequencies to Cross-Ancestry Association Studies.
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Lazareva, Tatyana E., Barbitoff, Yury A., Changalidis, Anton I., Tkachenko, Alexander A., Maksiutenko, Evgeniia M., Nasykhova, Yulia A., and Glotov, Andrey S.
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GENE frequency , *GENETIC epidemiology , *DATA integration , *GENOME-wide association studies , *HUMAN genome - Abstract
In recent years, great advances have been made in the field of collection, storage, and analysis of biological samples. Large collections of samples, biobanks, have been established in many countries. Biobanks typically collect large amounts of biological samples and associated clinical information; the largest collections include over a million samples. In this review, we summarize the main directions in which biobanks aid medical genetics and genomic research, from providing reference allele frequency information to allowing large-scale cross-ancestry meta-analyses. The largest biobanks greatly vary in the size of the collection, and the amount of available phenotype and genotype data. Nevertheless, all of them are extensively used in genomics, providing a rich resource for genome-wide association analysis, genetic epidemiology, and statistical research into the structure, function, and evolution of the human genome. Recently, multiple research efforts were based on trans-biobank data integration, which increases sample size and allows for the identification of robust genetic associations. We provide prominent examples of such data integration and discuss important caveats which have to be taken into account in trans-biobank research. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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19. Aggregation of Genome-Wide Association Data from FinnGen and UK Biobank Replicates Multiple Risk Loci for Pregnancy Complications.
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Changalidis, Anton I., Maksiutenko, Evgeniia M., Barbitoff, Yury A., Tkachenko, Alexander A., Vashukova, Elena S., Pachuliia, Olga V., Nasykhova, Yulia A., and Glotov, Andrey S.
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PREGNANCY complications ,GENOME-wide association studies ,HYPERTENSION in pregnancy ,PREMATURE labor ,GENETIC correlations ,GESTATIONAL diabetes ,PREECLAMPSIA - Abstract
Complications endangering mother or fetus affect around one in seven pregnant women. Investigation of the genetic susceptibility to such diseases is of high importance for better understanding of the disease biology as well as for prediction of individual risk. In this study, we collected and analyzed GWAS summary statistics from the FinnGen cohort and UK Biobank for 24 pregnancy complications. In FinnGen, we identified 11 loci associated with pregnancy hypertension, excessive vomiting, and gestational diabetes. When UK Biobank and FinnGen data were combined, we discovered six loci reaching genome-wide significance in the meta-analysis. These include rs35954793 in FGF5 ( p = 6.1 × 10 − 9 ), rs10882398 in PLCE1 ( p = 8.9 × 10 − 9 ), and rs167479 in RGL3 ( p = 5.2 × 10 − 9 ) for pregnancy hypertension, rs10830963 in MTNR1B ( p = 4.5 × 10 − 41 ) and rs36090025 in TCF7L2 ( p = 3.4 × 10 − 15 ) for gestational diabetes, and rs2963457 in the EBF1 locus ( p = 6.5 × 10 − 9 ) for preterm birth. In addition to the identified genome-wide associations, we also replicated 14 out of 40 previously reported GWAS markers for pregnancy complications, including four more preeclampsia-related variants. Finally, annotation of the GWAS results identified a causal relationship between gene expression in the cervix and gestational hypertension, as well as both known and previously uncharacterized genetic correlations between pregnancy complications and other traits. These results suggest new prospects for research into the etiology and pathogenesis of pregnancy complications, as well as early risk prediction for these disorders. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Processing of Fluorescent Proteins May Prevent Detection of Prion Particles in [ PSI + ] Cells.
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Matveenko, Andrew G., Ryzhkova, Varvara E., Zaytseva, Natalia A., Danilov, Lavrentii G., Mikhailichenko, Anastasia S., Barbitoff, Yury A., and Zhouravleva, Galina A.
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FLUORESCENT proteins ,PRIONS ,GREEN fluorescent protein ,STOP codons ,PROTEOLYSIS ,FLUORESCENCE microscopy - Abstract
Simple Summary: Prions are self-perpetuating protein aggregates that cause fatal neurodegenerative diseases in humans and other mammalian species. In yeast, in contrast to mammals, prions can be maintained in the cell population and confer adaptive traits. Fluorescence microscopy is commonly used to visualize prion aggregates in living cells, providing important information regarding the morphology and localization of prion particles in the cell. In most studies, various constructs with green fluorescent protein (GFP) are used to detect particles of the most-studied yeast prion, [PSI
+ ]. In our work, we tried to substitute GFP with two different red fluorescent protein variants to expand the application of prion particle imaging. Surprisingly, we found that the processing of the fluorescently labeled prionogenic protein can prevent the detection of prion particles. This pattern was observed for one of the studied red fluorescent proteins (mCherry) and was not dependent on any tested protein degradation systems. The present work thus highlights the limitations of aggregate labeling with fluorescent proteins and suggests labeling with mCherry should be avoided. Yeast is a convenient model for studying protein aggregation as it is known to propagate amyloid prions. [PSI+ ] is the prion form of the release factor eRF3 (Sup35). Aggregated Sup35 causes defects in termination of translation, which results in nonsense suppression in strains carrying premature stop codons. N-terminal and middle (M) domains of Sup35 are necessary and sufficient for maintaining [PSI+ ] in cells while preserving the prion strain's properties. For this reason, Sup35NM fused to fluorescent proteins is often used for [PSI+ ] detection and investigation. However, we found that in such chimeric constructs, not all fluorescent proteins allow the reliable detection of Sup35 aggregates. Particularly, transient overproduction of Sup35NM-mCherry resulted in a diffuse fluorescent pattern in the [PSI+ ] cells, while no loss of prions and no effect on the Sup35NM prion properties could be observed. This effect was reproduced in various unrelated strain backgrounds and prion variants. In contrast, Sup35NM fused to another red fluorescent protein, TagRFP-T, allowed the detection of [PSI+ ] aggregates. Analysis of protein lysates showed that Sup35NM-mCherry is actively degraded in the cell. This degradation was not caused by vacuolar proteases and the ubiquitin-proteasomal system implicated in the Sup35 processing. Even though the intensity of this proteolysis was higher than that of Sup35NM-GFP, it was roughly the same as in the case of Sup35NM-TagRFP-T. Thus, it is possible that, in contrast to TagRFP-T, degradation products of Sup35NM-mCherry still preserve their fluorescent properties while losing the ability to decorate pre-existing Sup35 aggregates. This results in diffuse fluorescence despite the presence of the prion aggregates in the cell. Thus, tagging with fluorescent proteins should be used with caution, as such proteolysis may increase the rate of false-negative results when detecting prion-bearing cells. [ABSTRACT FROM AUTHOR]- Published
- 2022
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21. Genetic and Phenotypic Factors Affecting Glycemic Response to Metformin Therapy in Patients with Type 2 Diabetes Mellitus.
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Nasykhova, Yulia A., Barbitoff, Yury A., Tonyan, Ziravard N., Danilova, Maria M., Nevzorov, Ivan A., Komandresova, Tatiana M., Mikhailova, Anastasiia A., Vasilieva, Tatiana V., Glavnova, Olga B., Yarmolinskaya, Maria I., Sluchanko, Evgenia I., and Glotov, Andrey S.
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- *
HYPOGLYCEMIC agents , *TYPE 2 diabetes , *GENETIC polymorphisms , *TREATMENT effectiveness , *PHENOTYPES , *GENETIC variation , *PHARMACOEPIDEMIOLOGY , *WAIST-hip ratio - Abstract
Metformin is an oral hypoglycemic agent widely used in clinical practice for treatment of patients with type 2 diabetes mellitus (T2DM). The wide interindividual variability of response to metformin therapy was shown, and recently the impact of several genetic variants was reported. To assess the independent and combined effect of the genetic polymorphism on glycemic response to metformin, we performed an association analysis of the variants in ATM, SLC22A1, SLC47A1, and SLC2A2 genes with metformin response in 299 patients with T2DM. Likewise, the distribution of allele and genotype frequencies of the studied gene variants was analyzed in an extended group of patients with T2DM (n = 464) and a population group (n = 129). According to our results, one variant, rs12208357 in the SLC22A1 gene, had a significant impact on response to metformin in T2DM patients. Carriers of TT genotype and T allele had a lower response to metformin compared to carriers of CC/CT genotypes and C allele (p-value = 0.0246, p-value = 0.0059, respectively). To identify the parameters that had the greatest importance for the prediction of the therapy response to metformin, we next built a set of machine learning models, based on the various combinations of genetic and phenotypic characteristics. The model based on a set of four parameters, including gender, rs12208357 genotype, familial T2DM background, and waist–hip ratio (WHR) showed the highest prediction accuracy for the response to metformin therapy in patients with T2DM (AUC = 0.62 in cross-validation). Further pharmacogenetic studies may aid in the discovery of the fundamental mechanisms of type 2 diabetes, the identification of new drug targets, and finally, it could advance the development of personalized treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. Overview of Transcriptomic Research on Type 2 Diabetes: Challenges and Perspectives.
- Author
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Tonyan, Ziravard N., Nasykhova, Yulia A., Danilova, Maria M., Barbitoff, Yury A., Changalidi, Anton I., Mikhailova, Anastasiia A., and Glotov, Andrey S.
- Subjects
TYPE 2 diabetes ,TRANSCRIPTOMES ,GENETIC variation ,GENE expression ,MICROARRAY technology - Abstract
Type 2 diabetes (T2D) is a common chronic disease whose etiology is known to have a strong genetic component. Standard genetic approaches, although allowing for the detection of a number of gene variants associated with the disease as well as differentially expressed genes, cannot fully explain the hereditary factor in T2D. The explosive growth in the genomic sequencing technologies over the last decades provided an exceptional impetus for transcriptomic studies and new approaches to gene expression measurement, such as RNA-sequencing (RNA-seq) and single-cell technologies. The transcriptomic analysis has the potential to find new biomarkers to identify risk groups for developing T2D and its microvascular and macrovascular complications, which will significantly affect the strategies for early diagnosis, treatment, and preventing the development of complications. In this article, we focused on transcriptomic studies conducted using expression arrays, RNA-seq, and single-cell sequencing to highlight recent findings related to T2D and challenges associated with transcriptome experiments. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
23. Molecular Genetics and Pathogenesis of the Floating Harbor Syndrome: Case Report of Long-Term Growth Hormone Treatment and a Literature Review.
- Author
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Turkunova, Mariia E., Barbitoff, Yury A., Serebryakova, Elena A., Polev, Dmitrii E., Berseneva, Olga S., Bashnina, Elena B., Baranov, Vladislav S., Glotov, Oleg S., and Glotov, Andrey S.
- Subjects
MOLECULAR genetics ,SOMATOTROPIN ,PITUITARY dwarfism ,SHORT stature ,GENETIC variation - Abstract
Introduction: Floating Harbor syndrome (FHS) is an extremely rare disorder, with slightly more than a hundred cases reported worldwide. FHS is caused by heterozygous mutations in the SRCAP gene; however, little is known about the pathogenesis of FHS or the effectiveness of its treatment. Methods: Whole-exome sequencing (WES) was performed for the definitive molecular diagnosis of the disease. Identified variants were validated using Sanger sequencing. In addition, systematic literature and public data on genetic variation in SRCAP and the effects of growth hormone (GH) treatment was conducted. Results: We herein report the first case of FHS in the Russian Federation. The male proband presented with most of the typical phenotypic features of FHS, including short stature, skeletal and facial features, delayed growth and bone age, high pitched voice, and intellectual impairment. The proband also had partial growth hormone deficiency. We report the history of treatment of the proband with GH, which resulted in modest improvement in growth prior to puberty. WES revealed a pathogenic c.7466C>G (p.Ser2489*) mutation in the last exon of the FHS-linked SRCAP gene. A systematic literature review and analysis of available genetic variation datasets highlighted an unusual distribution of pathogenic variants in SRCAP and confirmed the lack of pathogenicity for variants outside of exons 33 and 34. Finally, we suggested a new model of FHS pathogenesis which provides possible basis for the dominant negative nature of FHS-causing mutations and explains limited effects of GH treatment in FHS. Conclusion: Our findings expand the number of reported FHS cases and provide new insights into disease genetics and the efficiency of GH therapy for FHS patients. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
24. Structure and Polymorphism of Amyloid and Amyloid-Like Aggregates.
- Author
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Matiiv, Anton B., Trubitsina, Nina P., Matveenko, Andrew G., Barbitoff, Yury A., Zhouravleva, Galina A., and Bondarev, Stanislav A.
- Subjects
PROTEIN conformation ,PROTEIN folding ,AMYLOID beta-protein ,NEURODEGENERATION - Abstract
Amyloids are protein aggregates with the cross-β structure. The interest in amyloids is explained, on the one hand, by their role in the development of socially significant human neurodegenerative diseases, and on the other hand, by the discovery of functional amyloids, whose formation is an integral part of cellular processes. To date, more than a hundred proteins with the amyloid or amyloid-like properties have been identified. Studying the structure of amyloid aggregates has revealed a wide variety of protein conformations. In the review, we discuss the diversity of protein folds in the amyloid-like aggregates and the characteristic features of amyloid aggregates that determine their unusual properties, including stability and interaction with amyloid-specific dyes. The review also describes the diversity of amyloid aggregates and its significance for living organisms. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
25. RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters.
- Author
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Glotov, Andrey S., Zelenkova, Irina E., Vashukova, Elena S., Shuvalova, Anna R., Zolotareva, Alexandra D., Polev, Dmitrii E., Barbitoff, Yury A., Glotov, Oleg S., Sarana, Andrey M., Shcherbak, Sergey G., Rozina, Mariya A., Gogotova, Victoria L., and Predeus, Alexander V.
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RNA sequencing ,ELITE athletes ,ALTITUDES ,GENE expression ,ENDURANCE athletes ,OXYGENATORS - Abstract
Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profiling of whole blood. Seven elite female speed skaters were profiled on the 18th day of high-altitude adaptation. Whole blood RNA-seq before and after an intense 1 h skating bout was used to measure gene expression changes associated with exercise. In order to identify the genes specifically regulated at high altitudes, we have leveraged the data from eight previously published microarray datasets studying blood expression changes after exercise at sea level. Using cell type-specific signatures, we were able to deconvolute changes of cell type abundance from individual gene expression changes. Among these were PHOSPHO1, with a known role in erythropoiesis, and MARC1 with a role in endogenic NO metabolism. We find that platelet and erythrocyte counts uniquely respond to altitude exercise, while changes in neutrophils represent a more generic marker of intense exercise. Publicly available data from both single cell atlases and exercise-related blood profiling dramatically increases the value of whole blood RNA-seq for the dynamic evaluation of physiological changes in an athlete's body. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
26. Identification of Genetic Risk Factors of Severe COVID-19 Using Extensive Phenotypic Data: A Proof-of-Concept Study in a Cohort of Russian Patients.
- Author
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Shcherbak, Sergey G., Changalidi, Anton I., Barbitoff, Yury A., Anisenkova, Anna Yu., Mosenko, Sergei V., Asaulenko, Zakhar P., Tsay, Victoria V., Polev, Dmitrii E., Kalinin, Roman S., Eismont, Yuri A., Glotov, Andrey S., Garbuzov, Evgeny Y., Chernov, Alexander N., Klitsenko, Olga A., Ushakov, Mikhail O., Shikov, Anton E., Urazov, Stanislav P., Baranov, Vladislav S., and Glotov, Oleg S.
- Abstract
The COVID-19 pandemic has drawn the attention of many researchers to the interaction between pathogen and host genomes. Over the last two years, numerous studies have been conducted to identify the genetic risk factors that predict COVID-19 severity and outcome. However, such an analysis might be complicated in cohorts of limited size and/or in case of limited breadth of genome coverage. In this work, we tried to circumvent these challenges by searching for candidate genes and genetic variants associated with a variety of quantitative and binary traits in a cohort of 840 COVID-19 patients from Russia. While we found no gene- or pathway-level associations with the disease severity and outcome, we discovered eleven independent candidate loci associated with quantitative traits in COVID-19 patients. Out of these, the most significant associations correspond to rs1651553 in MYH14p = 1.4 × 10
−7 ), rs11243705 in SETX (p = 8.2 × 10−6 ), and rs16885 in ATXN1 (p = 1.3 × 10−5 ). One of the identified variants, rs33985936 in SCN11A, was successfully replicated in an independent study, and three of the variants were found to be associated with blood-related quantitative traits according to the UK Biobank data (rs33985936 in SCN11A, rs16885 in ATXN1, and rs4747194 in CDH23). Moreover, we show that a risk score based on these variants can predict the severity and outcome of hospitalization in our cohort of patients. Given these findings, we believe that our work may serve as proof-of-concept study demonstrating the utility of quantitative traits and extensive phenotyping for identification of genetic risk factors of severe COVID-19. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
27. Direct proof of the amyloid nature of yeast prions [PSI+] and [PIN+] by the method of immunoprecipitation of native fibrils.
- Author
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Sergeeva, Aleksandra V, Belashova, Tatyana A, Bondarev, Stanislav A, Velizhanina, Marya E, Barbitoff, Yury A, Matveenko, Andrew G, Valina, Anna A, Simanova, Angelina L, Zhouravleva, Galina A, and Galkin, Alexey P
- Subjects
PRIONS ,AMYLOID ,CONGO red (Staining dye) ,IMMUNOPRECIPITATION ,YEAST ,ELECTRON microscopy - Abstract
Prions are proteins that can exist in several structurally and functionally distinct states, one or more of which is transmissible. Yeast proteins Sup35 and Rnq1 in prion state ([ PSI
+ ] and [ PIN+ ], respectively) form oligomers and aggregates, which are transmitted from parents to offspring in a series of generations. Several pieces of indirect evidence indicate that these aggregates also possess amyloid properties, but their binding to amyloid-specific dyes has not been shown in vivo. Meanwhile, it is the specific binding to the Congo Red dye and birefringence in polarized light after such staining that is considered the gold standard for proving the amyloid properties of a protein. Here, we used immunoprecipitation to extract native fibrils of the Sup35 and Rnq1 proteins from yeast strains with different prion status. These fibrils are detected by electron microscopy, stained with Congo Red and exhibit yellow-green birefringence after such staining. All these data show that the Sup35 and Rnq1 proteins in prion state form amyloid fibrils in vivo. The technology of fibrils extraction in combination with standard cytological methods can be used to identify new pathological and functional amyloids in any organism and to analyze the structural features of native amyloid fibrils. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
28. Chromosome-level genome assembly and structural variant analysis of two laboratory yeast strains from the Peterhof Genetic Collection lineage.
- Author
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Barbitoff, Yury A., Matveenko, Andrew G., Matiiv, Anton B., Maksiutenko, Evgeniia M., Moskalenko, Svetlana E., Polev, Polina B. Drozdova,4 Dmitrii E., Beliavskaia, Alexandra Y., Danilov, Lavrentii G., Predeus, Alexander V., and Zhouravleva, Galina A.
- Subjects
- *
GENOMES , *YEAST , *CHROMOSOMAL rearrangement , *CHROMOSOMES , *CHROMOSOMAL translocation - Abstract
Thousands of yeast genomes have been sequenced with both traditional and long-read technologies, and multiple observations about modes of genome evolution for both wild and laboratory strains have been drawn from these sequences. In our study, we applied Oxford Nanopore and Illumina technologies to assemble complete genomes of two widely used members of a distinct laboratory yeast lineage, the Peterhof Genetic Collection (PGC), and investigate the structural features of these genomes including transposable element content, copy number alterations, and structural rearrangements. We identified numerous notable structural differences between genomes of PGC strains and the reference S288C strain. We discovered a substantial enrichment of mid-length insertions and deletions within repetitive coding sequences, such as in the SCH9 gene or the NUP100 gene, with possible impact of these variants on protein amyloidogenicity. High contiguity of the final assemblies allowed us to trace back the history of reciprocal unbalanced translocations between chromosomes I, VIII, IX, XI, and XVI of the PGC strains. We show that formation of hybrid alleles of the FLO genes during such chromosomal rearrangements is likely responsible for the lack of invasive growth of yeast strains. Taken together, our results highlight important features of laboratory yeast strain evolution using the power of long-read sequencing. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
29. P444: SINGLE CELL RNASEQ UNCOVERING THE ROLE OF A LEUKEMIC DRIVER GENE FUSION CBFΒ::MYH11 IN THE LEUKEMIC–NICHE SIGNALING.
- Author
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Derevyanko, Polina, V Dolgikh, Alexandra, Ashtiani, Minoo, Krippner‐Heidenreich, Anja, Nelson, Ryan, Bushweller, John, Barbitoff, Yury, V Predeus, Alexander, and Heidenreich, Olaf
- Published
- 2023
- Full Text
- View/download PDF
30. Identification of novel variants in the LDLR gene in Russian patients with familial hypercholesterolemia using targeted sequencing.
- Author
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MIROSHNIKOVA, VALENTINA V., ROMANOVA, OLGA V., IVANOVA, OLGA N., FEDYAKOV, MIKHAIL A., PANTELEEVA, ALEXANDRA A., BARBITOFF, YURY A., MUZALEVSKAYA, MARIA V., URAZGILDEEVA, SOREJYA A., GUREVICH, VICTOR S., URAZOV, STANISLAV P., SCHERBAK, SERGEY G., SARANA, ANDREY M., SEMENOVA, NATALIA A., ANISIMOVA, INGA V., GUSEVA, DARYA M., PCHELINA, SOFYA N., GLOTOV, ANDREY S., ZAKHAROVA, EKATERINA Y., and GLOTOV, OLEG S.
- Subjects
GENES ,HYPERCHOLESTEREMIA ,AGE groups ,PROTEIN domains ,RUSSIANS ,FAMILIAL hypercholesterolemia ,RECESSIVE genes - Abstract
Familial hypercholesterolemia (FH) is caused by mutations in various genes, including the LDLR, APOB and PSCK9 genes; however, the spectrum of these mutations in Russian individuals has not been fully investigated. In the present study, mutation screening was performed on the LDLR gene and other FH‑associated genes in patients with definite or possible FH, using next‑generation sequencing. In total, 59 unrelated patients were recruited and sorted into two separate groups depending on their age: Adult (n=31; median age, 49; age range, 23‑70) and children/adolescent (n=28; median age, 11; age range, 2‑21). FH‑associated variants were identified in 18 adults and 25 children, demonstrating mutation detec‑ tion rates of 58 and 89% for the adult and children/adolescent groups, respectively. In the adult group, 13 patients had FH‑associated mutations in the LDLR gene, including two novel variants [NM_000527.4: c.433_434dupG p.(Val145Glyfs*35) and c.1186G>C p.(Gly396Arg)], 3 patients had APOB mutations and two had ABCG5/G8 mutations. In the chil‑ dren/adolescent group, 21 patients had FH‑causing mutations in the LDLR gene, including five novel variants [NM_000527.4: c.325T>G p.(Cys109Gly), c.401G>C p.(Cys134Ser), c.616A>C p.(Ser206Arg), c.1684_1691delTGGCCCAA p.(Pro563Hisfs*14) and c.940+1_c.940+4delGTGA], and 2 patients had APOB mutations, as well as ABCG8 and LIPA mutations, being found in different patients. The present study reported seven novel LDLR variants considered to be patho‑ genic or likely pathogenic. Among them, four missense variants were located in the coding regions, which corresponded to functional protein domains, and two frameshifts were identified that produced truncated proteins. These variants were observed only once in different patients, whereas a splicing variant in intron 6 (c.940+1_c.940+4delGTGA) was detected in four unre‑ lated individuals. Previously reported variants in the LDLR, APOB, ABCG5/8 and LIPA genes were observed in 33 patients. The LDLR p.(Gly592Glu) variant was detected in 6 patients, representing 10% of the FH cases reported in the present study, thus it may be a major variant present in the Russian population. In conclusion, the present study identified seven novel variants of the LDLR gene and broadens the spectrum of mutations in FH‑related genes in the Russian Federation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
31. Analysis of the Spectrum of ACE2 Variation Suggests a Possible Influence of Rare and Common Variants on Susceptibility to COVID-19 and Severity of Outcome.
- Author
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Shikov, Anton E., Barbitoff, Yury A., Glotov, Andrey S., Danilova, Maria M., Tonyan, Ziravard N., Nasykhova, Yulia A., Mikhailova, Anastasia A., Bespalova, Olesya N., Kalinin, Roman S., Mirzorustamova, Azizahon M., Kogan, Igor Yu, Baranov, Vladislav S., Chernov, Alexander N., Pavlovich, Dragana M., Azarenko, Sergey V., Fedyakov, Mikhail A., Tsay, Victoria V., Eismont, Yuri A., Romanova, Olga V., and Hobotnikov, Dmitry N.
- Subjects
COVID-19 ,SPECTRUM analysis ,PANDEMICS ,SARS-CoV-2 ,RESPIRATORY diseases ,COMMUNICABLE diseases ,ANGIOTENSIN converting enzyme - Abstract
Objectives: In March 2020, the World Health Organization declared that an infectious respiratory disease caused by a new severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, causing coronavirus disease 2019 (COVID-19)] became a pandemic. In our study, we have analyzed a large publicly available dataset, the Genome Aggregation Database (gnomAD), as well as a cohort of 37 Russian patients with COVID-19 to assess the influence of different classes of genetic variants in the angiotensin-converting enzyme-2 (ACE2) gene on the susceptibility to COVID-19 and the severity of disease outcome. Results: We demonstrate that the European populations slightly differ in alternative allele frequencies at the 2,754 variant sites in ACE2 identified in the gnomAD database. We find that the Southern European population has a lower frequency of missense variants and slightly higher frequency of regulatory variants. However, we found no statistical support for the significance of these differences. We also show that the Russian population is similar to other European populations when comparing the frequencies of the ACE2 variants. Evaluation of the effect of various classes of ACE2 variants on COVID-19 outcome in a cohort of Russian patients showed that common missense and regulatory variants do not explain the differences in disease severity. At the same time, we find several rare ACE2 variants (including rs146598386, rs73195521, rs755766792, and others) that are likely to affect the outcome of COVID-19. Our results demonstrate that the spectrum of genetic variants in ACE2 may partially explain the differences in severity of the COVID-19 outcome. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
32. Quantitative assessment of chaperone binding to amyloid aggregates identifies specificity of Hsp40 interaction with yeast prion fibrils.
- Author
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Barbitoff, Yury A, Matveenko, Andrew G, Bondarev, Stanislav A, Maksiutenko, Evgeniia M, Kulikova, Alexandra V, and Zhouravleva, Galina A
- Subjects
- *
PRIONS , *YEAST , *MOLECULAR chaperones , *AMYLOID beta-protein , *QUALITY control , *PROTEIN-protein interactions , *MAGNITUDE (Mathematics) - Abstract
Yeast self-perpetuating protein aggregates (yeast prions) provide a framework to investigate the interaction of misfolded proteins with the protein quality control machinery. The major component of this system that facilitates propagation of all known yeast amyloid prions is the Hsp104 chaperone that catalyzes fibril fragmentation. Overproduction of Hsp104 cures some yeast prions via a fragmentation-independent mechanism. Importantly, major cytosolic chaperones of the Hsp40 group, Sis1 and Ydj1, oppositely affect yeast prion propagation, and are capable of stimulating different activities of Hsp104. In this work, we developed a quantitative method to investigate the Hsp40 binding to amyloid aggregates. We demonstrate that Sis1 binds fibrils formed by the Sup35NM protein with higher affinity compared to Ydj1. Moreover, the interaction of Sis1 with the fibrils formed by the other yeast prion protein, Rnq1, is orders of magnitude weaker. We show that the deletion of the dimerization domain of Sis1 (crucial for the curing of [ PSI +] by excess Hsp104) decreases its affinity to both Sup35NM and Rnq1 fibrils. Taken together, these results suggest that tight binding of Hsp40 to the amyloid fibrils is likely to enhance aggregate malpartition instead of fibril fragmentation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
33. Estimation of amyloid aggregate sizes with semi-denaturing detergent agarose gel electrophoresis and its limitations.
- Author
-
Drozdova, Polina B., Barbitoff, Yury A., Belousov, Mikhail V., Skitchenko, Rostislav K., Rogoza, Tatyana M., Leclercq, Jeremy Y., Kajava, Andrey V., Matveenko, Andrew G., Zhouravleva, Galina A., and Bondarev, Stanislav A.
- Published
- 2020
- Full Text
- View/download PDF
34. Whole-exome sequencing in Russian children with non-type 1 diabetes mellitus reveals a wide spectrum of genetic variants in MODY-related and unrelated genes.
- Author
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Glotov, Oleg S., Serebryakova, Elena A., Turkunova, Mariia E., Efimova, Olga A., Glotov, Andrey S., Barbitoff, Yury A., Nasykhova, Yulia A., Predeus, Alexander V., Polev, Dmitrii E., Fedyakov, Mikhail A., Polyakova, Irina V., Ivashchenko, Tatyana E., Shved, Natalia Y., Shabanova, Elena S., Tiselko, Alena V., Romanova, Olga V., Sarana, Andrey M., Pendina, Anna A., Scherbak, Sergey G., and Musina, Ekaterina V.
- Subjects
TYPE 2 diabetes ,DIABETES ,DIABETES in children ,EXOMES ,GENES ,FREQUENCY spectra ,MATURITY onset diabetes of the young - Abstract
The present study reports on the frequency and the spectrum of genetic variants causative of monogenic diabetes in Russian children with non-type 1 diabetes mellitus. The present study included 60 unrelated Russian children with non-type 1 diabetes mellitus diagnosed before the age of 18 years. Genetic variants were screened using whole-exome sequencing (WES) in a panel of 35 genes causative of maturity onset diabetes of the young (MODY) and transient or permanent neonatal diabetes. Verification of the WES results was performed using PCR-direct sequencing. A total of 38 genetic variants were identified in 33 out of 60 patients (55%). The majority of patients (27/33, 81.8%) had variants in MODY-related genes: GCK (n=19), HNF1A (n=2), PAX4 (n=1), ABCC8 (n=1), KCNJ11 (n=1), GCK+HNF1A (n=1), GCK+BLK (n=1) and GCK+BLK+WFS1 (n=1). A total of 6 patients (6/33, 18.2%) had variants in MODY-unrelated genes: GATA6 (n=1), WFS1 (n=3), EIF2AK3 (n=1) and SLC19A2 (n=1). A total of 15 out of 38 variants were novel, including GCK, HNF1A, BLK, WFS1, EIF2AK3 and SLC19A2. To summarize, the present study demonstrates a high frequency and a wide spectrum of genetic variants causative of monogenic diabetes in Russian children with non-type 1 diabetes mellitus. The spectrum includes previously known and novel variants in MODY-related and unrelated genes, with multiple variants in a number of patients. The prevalence of GCK variants indicates that diagnostics of monogenic diabetes in Russian children may begin with testing for MODY2. However, the remaining variants are present at low frequencies in 9 different genes, altogether amounting to ~50% of the cases and highlighting the efficiency of using WES in non-GCK-MODY cases. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
35. Differential effects of chaperones on yeast prions: CURrent view.
- Author
-
Matveenko, Andrew G., Barbitoff, Yury A., Jay-Garcia, Lina Manuela, Chernoff, Yury O., and Zhouravleva, Galina A.
- Subjects
- *
YEAST , *PRIONS , *SACCHAROMYCES cerevisiae , *MOLECULAR chaperones , *PROTEIN folding - Abstract
Endogenous yeast amyloids that control heritable traits and are frequently used as models for human amyloid diseases are termed yeast prions. Yeast prions, including the best studied ones ([PSI+] and [URE3]), propagate via intimate interactions with molecular chaperones. Different yeast prions exhibit differential responses to changes in levels, functionality or localization of the components of chaperone machinery. Here, we provide additional data confirming differential effects of chaperones (and specifically, Hsp40s) on yeast prions and summarize current knowledge of the mechanisms underlying chaperone specificities. Contrary to frequent statements in literature, overproduction of the Hsp104 chaperone antagonizes both [PSI+] and [URE3] prions, while overproduction of the Hsp70-Ssa1 chaperone antagonizes [URE3] prion only in some, but not in all strains. Recently, we demonstrated that the relocalization of a fraction of the Hsp40 chaperone Sis1 from the cytosol to the nucleus by the chaperone-sorting factor Cur1 exhibits opposite effects on [PSI+] and [URE3] prions. We suggest that the response of prions to changes in Sis1 localization represents a combination of the effects of Sis1 shortage on fragmentation of prion aggregates and on malpartition of prion aggregates during a cell division. Differences in sensitivity of prion fragmentation to Sis1 and in relative inputs of fragmentation and malpartition in prion propagation result in opposite effects of Sis1 relocalization on [PSI+] and [URE3]. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
36. To CURe or not to CURe? Differential effects of the chaperone sorting factor Cur1 on yeast prions are mediated by the chaperone Sis1.
- Author
-
Barbitoff, Yury A., Matveenko, Andrew G., Moskalenko, Svetlana E., Zemlyanko, Olga M., Newnam, Gary P., Patel, Ayesha, Chernova, Tatiana A., Chernoff, Yury O., and Zhouravleva, Galina A.
- Subjects
- *
PRIONS , *CURING , *YEAST , *MOLECULAR chaperones , *AMYLOID , *PROTEIN metabolism - Abstract
Yeast self-perpetuating protein aggregates (prions) provide a convenient model for studying various components of the cellular protein quality control system. Molecular chaperones and chaperone-sorting factors, such as yeast Cur1 protein, play key role in proteostasis via tight control of partitioning and recycling of misfolded proteins. In this study, we show that, despite the previously described ability of Cur1 to antagonize the yeast prion [ URE3], it enhances propagation and phenotypic manifestation of another prion, [ PSI+]. We demonstrate that both curing of [ URE3] and enhancement of [ PSI+] in the presence of excess Cur1 are counteracted by the cochaperone Hsp40-Sis1 in a dosage-dependent manner, and show that the effect of Cur1 on prions parallels effects of the attachment of nuclear localization signal to Sis1, indicating that Cur1 acts on prions via its previously reported ability to relocalize Sis1 from the cytoplasm to nucleus. This shows that the direction in which Cur1 influences a prion depends on how this specific prion responds to relocalization of Sis1. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
37. SFP1-mediated prion-dependent lethality is caused by increased Sup35 aggregation and alleviated by Sis1.
- Author
-
Matveenko, Andrew G., Drozdova, Polina B., Belousov, Mikhail V., Moskalenko, Svetlana E., Bondarev, Stanislav A., Barbitoff, Yury A., Nizhnikov, Anton A., and Zhouravleva, Galina A.
- Subjects
PRIONS ,TRANSCRIPTION factors ,SACCHAROMYCES cerevisiae ,GENETIC transcription regulation ,MONOMERS ,CLUSTERING of particles - Abstract
[ PSI
+ ] is the prion form of the translation termination factor Sup35 (eRF3); [ PSI+ ] strains display nonsense suppression. Another prion-like element, [ ISP+ ], is linked to antisuppression in a specific background. Transcriptional regulator Sfp1 was shown to be responsible for [ ISP+ ] propagation. In this work, we identified SFP1 as a multicopy inducer of [ PSI+ ]-dependent lethality. Sfp1 is likely to up-regulate transcription of genes encoding release factors; however, its overproduction increases Sup35, but not Sup45 protein level. Using the synthetic lethality test, we compared the effects of SFP1 and SUP35 over-expression on the viability of [ PSI+ ] strains. Together with an observation that Sfp1 overproduction leads to an increased accumulation of Sup35 in [ PSI+ ] aggregates, we suggest that excess Sfp1 causes [ PSI+ ] toxicity. Even though SUP45 over-expression is known to compensate for the [ PSI+ ]-dependent lethality, it fails to do so when the lethality is caused by SFP1 over-expression. We discovered that the increased levels of Hsp40 chaperone Sis1 alleviate prion toxicity caused by either SFP1 or SUP35 over-expression and revert back to normal distribution of Sup35 between monomers and aggregate fractions. Finally, we showed that Sfp1 partially colocalizes with Sup35 aggregates, which may contribute to another mechanism of Sfp1-derived [ PSI+ ] prion toxicity. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
38. Differential Interactions of Molecular Chaperones and Yeast Prions.
- Author
-
Barbitoff, Yury A., Matveenko, Andrew G., and Zhouravleva, Galina A.
- Subjects
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MOLECULAR chaperones , *SACCHAROMYCES cerevisiae , *PRIONS , *PROTEIN-protein interactions , *CELL communication , *PROTEIN folding - Abstract
Baker's yeast Saccharomyces cerevisiae is an important model organism that is applied to study various aspects of eukaryotic cell biology. Prions in yeast are self-perpetuating heritable protein aggregates that can be leveraged to study the interaction between the protein quality control (PQC) machinery and misfolded proteins. More than ten prions have been identified in yeast, of which the most studied ones include [PSI+], [URE3], and [PIN+]. While all of the major molecular chaperones have been implicated in propagation of yeast prions, many of these chaperones differentially impact propagation of different prions and/or prion variants. In this review, we summarize the current understanding of the life cycle of yeast prions and systematically review the effects of different chaperone proteins on their propagation. Our analysis clearly shows that Hsp40 proteins play a central role in prion propagation by determining the fate of prion seeds and other amyloids. Moreover, direct prion-chaperone interaction seems to be critically important for proper recruitment of all PQC components to the aggregate. Recent results also suggest that the cell asymmetry apparatus, cytoskeleton, and cell signaling all contribute to the complex network of prion interaction with the yeast cell. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
39. Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.
- Author
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Maksiutenko, Evgeniia M., Barbitoff, Yury A., Matveenko, Andrew G., Moskalenko, Svetlana E., and Zhouravleva, Galina A.
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NONSENSE mutation , *GENES , *GENETIC variation , *YEAST , *GENE amplification , *NUCLEOTIDE sequencing , *CHROMOSOME duplication - Abstract
Protein synthesis (translation) is one of the fundamental processes occurring in the cells of living organisms. Translation can be divided into three key steps: initiation, elongation, and termination. In the yeast Saccharomyces cerevisiae, there are two translation termination factors, eRF1 and eRF3. These factors are encoded by the SUP45 and SUP35 genes, which are essential; deletion of any of them leads to the death of yeast cells. However, viable strains with nonsense mutations in both the SUP35 and SUP45 genes were previously obtained in several groups. The survival of such mutants clearly involves feedback control of premature stop codon readthrough; however, the exact molecular basis of such feedback control remain unclear. To investigate the genetic factors supporting the viability of these SUP35 and SUP45 nonsense mutants, we performed whole-genome sequencing of strains carrying mutant sup35-n and sup45-n alleles; while no common SNPs or indels were found in these genomes, we discovered a systematic increase in the copy number of the plasmids carrying mutant sup35-n and sup45-n alleles. We used the qPCR method which confirmed the differences in the relative number of SUP35 and SUP45 gene copies between strains carrying wild-type or mutant alleles of SUP35 and SUP45 genes. Moreover, we compare the number of copies of the SUP35 and SUP45 genes in strains carrying different nonsense mutant variants of these genes as a single chromosomal copy. qPCR results indicate that the number of mutant gene copies is increased compared to the wild-type control. In case of several sup45-n alleles, this was due to a disomy of the entire chromosome II, while for the sup35-218 mutation we observed a local duplication of a segment of chromosome IV containing the SUP35 gene. Taken together, our results indicate that gene amplification is a common mechanism of adaptation to nonsense mutations in release factor genes in yeast. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
40. Development of SNP Set for the Marker-Assisted Selection of Guar (Cyamopsis tetragonoloba (L.) Taub.) Based on a Custom Reference Genome Assembly.
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Grigoreva, Elizaveta, Barbitoff, Yury, Changalidi, Anton, Karzhaev, Dmitry, Volkov, Vladimir, Shadrina, Veronika, Safronycheva, Elizaveta, Ben, Cécile, Gentzbittel, Laurent, and Potokina, Elena
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GUAR ,GUAR gum ,SINGLE nucleotide polymorphisms ,GENOME-wide association studies ,CROPS ,HARVESTING time - Abstract
Guar gum, a polysaccharide derived from guar seeds, is widely used in a variety of industrial applications, including oil and gas production. Although guar is mostly propagated in India, interest in guar as a new industrial legume crop is increasing worldwide, demanding the development of effective tools for marker-assisted selection. In this paper, we report a wide-ranging set of 4907 common SNPs and 327 InDels generated from RADseq genotyping data of 166 guar plants of different geographical origin. A custom guar reference genome was assembled and used for variant calling. A consensus set of variants was built using three bioinformatic pipelines for short variant discovery. The developed molecular markers were used for genome-wide association study, resulting in the discovery of six markers linked to the variation of an important agronomic trait—percentage of pods matured to the harvest date under long light day conditions. One of the associated variants was found inside the putative transcript sequence homologous to an ABC transporter in Arabidopsis, which has been shown to play an important role in D-myo-inositol phosphates metabolism. Earlier, we suggested that genes involved in myo-inositol phosphate metabolism have significant impact on the early flowering of guar plants. Hence, we believe that the developed SNP set allows for the identification of confident molecular markers of important agrobiological traits. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
41. A Data-Driven Review of the Genetic Factors of Pregnancy Complications.
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Barbitoff, Yury A., Tsarev, Alexander A., Vashukova, Elena S., Maksiutenko, Evgeniia M., Kovalenko, Liudmila V., Belotserkovtseva, Larisa D., and Glotov, Andrey S.
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PREGNANCY complications , *ABRUPTIO placentae , *GESTATIONAL diabetes , *PREMATURE labor , *GENOMICS , *HUMAN genome , *CELL adhesion - Abstract
Over the recent years, many advances have been made in the research of the genetic factors of pregnancy complications. In this work, we use publicly available data repositories, such as the National Human Genome Research Institute GWAS Catalog, HuGE Navigator, and the UK Biobank genetic and phenotypic dataset to gain insights into molecular pathways and individual genes behind a set of pregnancy-related traits, including the most studied ones—preeclampsia, gestational diabetes, preterm birth, and placental abruption. Using both HuGE and GWAS Catalog data, we confirm that immune system and, in particular, T-cell related pathways are one of the most important drivers of pregnancy-related traits. Pathway analysis of the data reveals that cell adhesion and matrisome-related genes are also commonly involved in pregnancy pathologies. We also find a large role of metabolic factors that affect not only gestational diabetes, but also the other traits. These shared metabolic genes include IGF2, PPARG, and NOS3. We further discover that the published genetic associations are poorly replicated in the independent UK Biobank cohort. Nevertheless, we find novel genome-wide associations with pregnancy-related traits for the FBLN7, STK32B, and ACTR3B genes, and replicate the effects of the KAZN and TLE1 genes, with the latter being the only gene identified across all data resources. Overall, our analysis highlights central molecular pathways for pregnancy-related traits, and suggests a need to use more accurate and sophisticated association analysis strategies to robustly identify genetic risk factors for pregnancy complications. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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42. Whole‐exome sequencing provides insights into monogenic disease prevalence in Northwest Russia.
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Barbitoff, Yury A., Skitchenko, Rostislav K., Poleshchuk, Olga I., Shikov, Anton E., Serebryakova, Elena A., Nasykhova, Yulia A., Polev, Dmitrii E., Shuvalova, Anna R., Shcherbakova, Irina V., Fedyakov, Mikhail A., Glotov, Oleg S., Glotov, Andrey S., and Predeus, Alexander V.
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DISEASE prevalence , *ALLELES , *HEPATOLENTICULAR degeneration , *GENE frequency , *EHLERS-Danlos syndrome , *RETINAL degeneration , *DISEASE incidence - Abstract
Background: Allele frequency data from large exome and genome aggregation projects such as the Genome Aggregation Database (gnomAD) are of ultimate importance to the interpretation of medical resequencing data. However, allele frequencies might significantly differ in poorly studied populations that are underrepresented in large‐scale projects, such as the Russian population. Methods: In this work, we leveraged our access to a large dataset of 694 exome samples to analyze genetic variation in the Northwest Russia. We compared the spectrum of genetic variants to the dbSNP build 151, and made estimates of ClinVar‐based autosomal recessive (AR) disease allele prevalence as compared to gnomAD r. 2.1. Results: An estimated 9.3% of discovered variants were not present in dbSNP. We report statistically significant overrepresentation of pathogenic variants for several Mendelian disorders, including phenylketonuria (PAH, rs5030858), Wilson's disease (ATP7B, rs76151636), factor VII deficiency (F7, rs36209567), kyphoscoliosis type of Ehlers‐Danlos syndrome (FKBP14, rs542489955), and several other recessive pathologies. We also make primary estimates of monogenic disease incidence in the population, with retinal dystrophy, cystic fibrosis, and phenylketonuria being the most frequent AR pathologies. Conclusion: Our observations demonstrate the utility of population‐specific allele frequency data to the diagnosis of monogenic disorders using high‐throughput technologies. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
43. Identification of Novel Candidate Markers of Type 2 Diabetes and Obesity in Russia by Exome Sequencing with a Limited Sample Size.
- Author
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Polev, Dmitrii E., Shuvalova, Anna R., Barbitoff, Yury A., Glotov, Andrey S., Nasykhova, Yulia A., Serebryakova, Elena A., Predeus, Alexander V., Glotov, Oleg S., Sarana, Andrey M., Scherbak, Sergey G., Vasiliev, Evgenii V., Urazov, Stanislav P., Gladyshev, Dmitrii V., Pokrovskaya, Maria S., Sivakova, Oksana V., Meshkov, Aleksey N., and Drapkina, Oxana M.
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TYPE 2 diabetes risk factors ,OBESITY risk factors ,INSULIN resistance ,HYPERGLYCEMIA ,HEART diseases ,BODY mass index ,NEUROPATHY - Abstract
Type 2 diabetes (T2D) and obesity are common chronic disorders with multifactorial etiology. In our study, we performed an exome sequencing analysis of 110 patients of Russian ethnicity together with a multi-perspective approach based on biologically meaningful filtering criteria to detect novel candidate variants and loci for T2D and obesity. We have identified several known single nucleotide polymorphisms (SNPs) as markers for obesity (rs11960429), T2D (rs9379084, rs1126930), and body mass index (BMI) (rs11553746, rs1956549 and rs7195386) (p < 0.05). We show that a method based on scoring of case-specific variants together with selection of protein-altering variants can allow for the interrogation of novel and known candidate markers of T2D and obesity in small samples. Using this method, we identified rs328 in LPL (p = 0.023), rs11863726 in HBQ1 (p = 8 × 10
−5 ), rs112984085 in VAV3 (p = 4.8 × 10−4 ) for T2D and obesity, rs6271 in DBH (p = 0.043), rs62618693 in QSER1 (p = 0.021), rs61758785 in RAD51B (p = 1.7 × 10−4 ), rs34042554 in PCDHA1 (p = 1 × 10−4 ), and rs144183813 in PLEKHA5 (p = 1.7 × 10−4 ) for obesity; and rs9379084 in RREB1 (p = 0.042), rs2233984 in C6orf15 (p = 0.030), rs61737764 in ITGB6 (p = 0.035), rs17801742 in COL2A1 (p = 8.5 × 10−5 ), and rs685523 in ADAMTS13 (p = 1 × 10−6 ) for T2D as important susceptibility loci in Russian population. Our results demonstrate the effectiveness of whole exome sequencing (WES) technologies for searching for novel markers of multifactorial diseases in cohorts of limited size in poorly studied populations. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
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