30 results on '"Barale, Jean-Christophe"'
Search Results
2. A surrogate marker of piperaquine-resistant Plasmodium falciparum malaria: a phenotype–genotype association study
- Author
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Witkowski, Benoit, Duru, Valentine, Khim, Nimol, Ross, Leila S, Saintpierre, Benjamin, Beghain, Johann, Chy, Sophy, Kim, Saorin, Ke, Sopheakvatey, Kloeung, Nimol, Eam, Rotha, Khean, Chanra, Ken, Malen, Loch, Kaknika, Bouillon, Anthony, Domergue, Anais, Ma, Laurence, Bouchier, Christiane, Leang, Rithea, Huy, Rekol, Nuel, Grégory, Barale, Jean-Christophe, Legrand, Eric, Ringwald, Pascal, Fidock, David A, Mercereau-Puijalon, Odile, Ariey, Frédéric, and Ménard, Didier
- Published
- 2017
- Full Text
- View/download PDF
3. Plasmodium falciparum Subtilisin-Like Protease 2, a Merozoite Candidate for the Merozoite Surface Protein 1-42 Maturase
- Author
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Barale, Jean-Christophe, Blisnick, Thierry, Fujioka, Hisashi, Alzari, Pedro M., Aikawa, Masamishi, Braun-Breton, Catherine, and Langsley, Gordon
- Published
- 1999
4. Mammalian Subtilisin/Kexin Isozyme SKI-1: A Widely Expressed Proprotein Convertase with a Unique Cleavage Specificity and Cellular Localization
- Author
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Seidah, Nabil G., Mowla, Seyed J., Hamelin, Josee, Mamarbachi, Aida M., Benjannet, Suzanne, Toure, Barry B., Basak, Ajoy, Munzer, Jon Scott, Marcinkiewicz, Jadwiga, Zhong, Mei, Barale, Jean-Christophe, Lazure, Claude, Murphy, Richard A., Chretien, Michel, and Marcinkiewicz, Mieczyslaw
- Published
- 1999
5. 3D structures of the Plasmodium vivax subtilisin‐like drug target SUB1 reveal conformational changes to accommodate a substrate‐derived α‐ketoamide inhibitor.
- Author
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Martinez, Mariano, Batista, Fernando A., Maurel, Manon, Bouillon, Anthony, Ortega Varga, Laura, Wehenkel, Anne Marie, Le Chevalier-Sontag, Lucile, Blondel, Arnaud, Haouz, Ahmed, Hernandez, Jean-François, Alzari, Pedro M., and Barale, Jean-Christophe
- Subjects
PLASMODIUM vivax ,DRUG target ,CATALYTIC domains ,LIFE cycles (Biology) ,HYDROGEN bonding interactions ,HYDROPHOBIC interactions ,PROTEOLYTIC enzymes - Abstract
The constant selection and propagation of multi‐resistant Plasmodium sp. parasites require the identification of new antimalarial candidates involved in as‐yet untargeted metabolic pathways. Subtilisin‐like protease 1 (SUB1) belongs to a new generation of drug targets because it plays a crucial role during egress of the parasite from infected host cells at different stages of its life cycle. SUB1 is characterized by an unusual pro‐region that tightly interacts with its cognate catalytic domain, thus precluding 3D structural analysis of enzyme–inhibitor complexes. In the present study, to overcome this limitation, stringent ionic conditions and controlled proteolysis of recombinant full‐length P. vivax SUB1 were used to obtain crystals of an active and stable catalytic domain (PvS1Cat) without a pro‐region. High‐resolution 3D structures of PvS1Cat, alone and in complex with an α‐ketoamide substrate‐derived inhibitor (MAM‐117), showed that, as expected, the catalytic serine of SUB1 formed a covalent bond with the α‐keto group of the inhibitor. A network of hydrogen bonds and hydrophobic interactions stabilized the complex, including at the P1′ and P2′ positions of the inhibitor, although P′ residues are usually less important in defining the substrate specificity of subtilisins. Moreover, when associated with a substrate‐derived peptidomimetic inhibitor, the catalytic groove of SUB1 underwent significant structural changes, particularly in its S4 pocket. These findings pave the way for future strategies for the design of optimized SUB1‐specific inhibitors that may define a novel class of antimalarial candidates. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
6. A small-molecule cell-based screen led to the identification of biphenylimidazoazines with highly potent and broad-spectrum anti-apicomplexan activity
- Author
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Moine, Espérance, Denevault-Sabourin, Caroline, Debierre-Grockiego, Françoise, Silpa, Laurence, Gorgette, Olivier, Barale, Jean-Christophe, Jacquiet, Philippe, Brossier, Fabien, Gueiffier, Alain, Dimier-Poisson, Isabelle, and Enguehard-Gueiffier, Cécile
- Published
- 2015
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- View/download PDF
7. Prevalence and characterization of piperaquine, mefloquine and artemisinin derivatives triple-resistant Plasmodium falciparum in Cambodia.
- Author
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Mairet-Khedim, Mélissa, Roesch, Camille, Khim, Nimol, Srun, Sreynet, Bouillon, Anthony, Kim, Saorin, Ke, Sopheakvatey, Kauy, Chhayleang, Kloeung, Nimol, Eam, Rotha, Khean, Chanra, Kul, Chanvong, Chy, Sophy, Leang, Rithea, Ringwald, Pascal, Barale, Jean-Christophe, and Witkowski, Benoit
- Subjects
ARTEMISININ derivatives ,MEFLOQUINE ,PLASMODIUM falciparum ,ARTEMISININ ,FUNGICIDE resistance - Abstract
Background In early 2016, in Preah Vihear, Northern Cambodia, artesunate/mefloquine was used to cope with dihydroartemisinin/piperaquine-resistant Plasmodium falciparum parasites. Following this policy, P. falciparum strains harbouring molecular markers associated with artemisinin, piperaquine and mefloquine resistance have emerged. However, the lack of a viable alternative led Cambodia to adopt artesunate/mefloquine countrywide, raising concerns about a surge of triple-resistant P. falciparum strains. Objectives To assess the prevalence of triple-resistant parasites after artesunate/mefloquine implementation countrywide in Cambodia and to characterize their phenotype. Methods For this multicentric study, 846 samples were collected from 2016 to 2019. Genotyping of molecular markers associated with artemisinin, piperaquine and mefloquine resistance was coupled with phenotypic analyses. Results Only four triple-resistant P. falciparum isolates (0.47%) were identified during the study period. These parasites combined the pfk13 polymorphism with pfmdr1 amplification, pfpm2 amplification and/or pfcrt mutations. They showed significantly higher tolerance to artemisinin, piperaquine and mefloquine and also to the mefloquine and piperaquine combination. Conclusions The use of artesunate/mefloquine countrywide in Cambodia has not led to a massive increase of triple-resistant P. falciparum parasites. However, these parasites circulate in the population, and exhibit clear resistance to piperaquine, mefloquine and their combination in vitro. This study demonstrates that P. falciparum can adapt to more complex drug associations, which should be considered in future therapeutic designs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
8. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
- Author
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Aney, Frederic, Witkowski, Benoit, Amaratunga, Chanaki, Beghain, Johann, Langlois, Anne-Claire, Khim, Nimol, Kim, Saorin, Duru, Valentine, Bouchier, Christiane, Ma, Laurence, Lim, Pharath, Leang, Rithea, Duong, Socheat, Sreng, Sokunthea, Suon, Seila, Chuor, Char Meng, Bout, Denis Mey, Menard, Sandie, Rogers, William O., Genton, Blaise, Fandeur, Thierry, Miotto, Olivo, Ringwald, Pascal, Bras, Jacques Le, Berry, Antoine, Barale, Jean-Christophe, Fairhurst, Rick M., Benoit-Vical, Francoise, Mercereau-Puijalon, Odile, and Menard, Didier
- Subjects
Drug resistance in microorganisms -- Research ,Molecular biology -- Research ,Plasmodium falciparum -- Physiological aspects -- Health aspects ,Microbiological research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7_1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread., The emergence of Plasmodium falciparum resistance to artemisinin derivatives (ART) in Cambodia threatens the world's malaria control and elimination efforts (1,2). The risk of ART-resistant parasites spreading from western Cambodia [...]
- Published
- 2014
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9. Plasmodium chabaudi p68 Serine Protease Activity Required for Merozoite Entry into Mouse Erythrocytes
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Breton, Catherine Braun, Blisnick, Thierry, Jouin, Helene, Barale, Jean Christophe, Rabilloud, Thierry, Rabilloud, Thierry, and Langsley, Gordon
- Published
- 1992
10. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
- Author
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Ariey, Frédéric, Witkowski, Benoit, Amaratunga, Chanaki, Beghain, Johann, Langlois, Anne-Claire, Khim, Nimol, Kim, Saorin, Duru, Valentine, Bouchier, Christiane, Ma, Laurence, Lim, Pharath, Leang, Rithea, Duong, Socheat, Sreng, Sokunthea, Suon, Seila, Chuor, Char Meng, Bout, Denis Mey, Ménard, Sandie, Rogers, William O., Genton, Blaise, Fandeur, Thierry, Miotto, Olivo, Ringwald, Pascal, Le Bras, Jacques, Berry, Antoine, Barale, Jean-Christophe, Fairhurst, Rick M., Benoit-Vical, Françoise, Mercereau-Puijalon, Odile, and Ménard, Didier
- Published
- 2014
- Full Text
- View/download PDF
11. Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites.
- Author
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Ashraf, Kutub, Tajeri, Shahin, Arnold, Christophe-Sébastien, Amanzougaghene, Nadia, Franetich, Jean-François, Vantaux, Amélie, Soulard, Valérie, Bordessoulles, Mallaury, Cazals, Guillaume, Bousema, Teun, van Gemert, Geert-Jan, Le Grand, Roger, Dereuddre-Bosquet, Nathalie, Barale, Jean-Christophe, Witkowski, Benoit, Snounou, Georges, Duval, Romain, Botté, Cyrille Y., and Mazier, Dominique
- Published
- 2022
- Full Text
- View/download PDF
12. Trypanosoma cruzi proline racemases are involved in parasite differentiation and infectivity
- Author
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Chamond, Nathalie, Goytia, Maira, Coatnoan, Nicolas, Barale, Jean-Christophe, Cosson, Alain, Degrave, Wim M., and Minoprio, Paola
- Published
- 2005
13. Three multigene families in Plasmodium parasites: facts and questions
- Author
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Mercereau-Puijalon, Odile, Barale, Jean-Christophe, and Bischoff, Emmanuel
- Published
- 2002
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14. CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use
- Author
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Mercereau-Puijalon Odile, Akanmori Bartholomew, Barale Jean-Christophe, Loizon Séverine, Jublot Delphine, Vigan-Womas Inès, Boeuf Philippe, and Behr Charlotte
- Subjects
Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background Real-time PCR is becoming a common tool for detecting and quantifying expression profiling of selected genes. Cytokines mRNA quantification is widely used in immunological research to dissect the early steps of immune responses or pathophysiological pathways. It is also growing to be of clinical relevancy to immuno-monitoring and evaluation of the disease status of patients. The techniques currently used for "absolute quantification" of cytokine mRNA are based on a DNA standard curve and do not take into account the critical impact of RT efficiency. Results To overcome this pitfall, we designed a strategy using external RNA as standard in the RT-PCR. Use of synthetic RNA standards, by comparison with the corresponding DNA standard, showed significant variations in the yield of retro-transcription depending the target amplified and the experiment. We then developed primers to be used under one single experimental condition for the specific amplification of human IL-1β, IL-4, IL-10, IL-12p40, IL-13, IL-15, IL-18, IFN-γ, MIF, TGF-β1 and TNF-α mRNA. We showed that the beta-2 microglobulin (β2-MG) gene was suitable for data normalisation since the level of β2-MG transcripts in naïve PBMC varied less than 5 times between individuals and was not affected by LPS or PHA stimulation. The technique, we named CyProQuant-PCR (Cytokine Profiling Quantitative PCR) was validated using a kinetic measurement of cytokine transcripts under in vitro stimulation of human PBMC by lipopolysaccharide (LPS) or Staphylococcus aureus strain Cowan (SAC). Results obtained show that CyProQuant-PCR is powerful enough to precociously detect slight cytokine induction. Finally, having demonstrated the reproducibility of the method, it was applied to malaria patients and asymptomatic controls for the quantification of TGF-β1 transcripts and showed an increased capacity of cells from malaria patients to accumulate TGF-β1 mRNA in response to LPS. Conclusion The real-time RT-PCR technique based on a RNA standard curve, CyProQuant-PCR, outlined here, allows for a genuine absolute quantification and a simultaneous analysis of a large panel of human cytokine mRNA. It represents a potent and attractive tool for immunomonitoring, lending itself readily to automation and with a high throughput. This opens the possibility of an easy and reliable cytokine profiling for clinical applications.
- Published
- 2005
- Full Text
- View/download PDF
15. In vitro activity of ferroquine against artemisinin-based combination therapy (ACT)-resistant Plasmodium falciparum isolates from Cambodia.
- Author
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Mairet-Khedim, Mélissa, Nardella, Flore, Khim, Nimol, Kim, Saorin, Kloeung, Nimol, Ke, Sopheakvatey, Kauy, Chhayleang, Eam, Rotha, Khean, Chanra, Pellet, Alain, Leboulleux, Didier, Leang, Rithea, Ringwald, Pascal, Barale, Jean Christophe, Leroy, Didier, Menard, Didier, and Witkowski, Benoit
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PLASMODIUM falciparum ,GENE amplification ,MALARIA ,HAPLOTYPES - Abstract
Background: Cambodia is the epicentre of resistance emergence for virtually all antimalarial drugs. Selection and spread of parasites resistant to artemisinin-based combination therapy (ACT) is a major threat for malaria elimination, hence the need to renew the pool of effective treatments.Objectives: To determine whether ACT resistance haplotypes could have an effect on ferroquine in vitro antimalarial activity.Methods: In vitro susceptibility to ferroquine was measured for 80 isolates from Cambodia characterized for their molecular resistance profile to artemisinin, piperaquine and mefloquine.Results: Among the 80 isolates tested, the overall median (IQR) IC50 of ferroquine was 10.9 nM (8.7-18.3). The ferroquine median (IQR) IC50 was 8.9 nM (8.1-11.8) for Pfk13 WT parasites and was 12.9 nM (9.5-20.0) for Pfk13 C580Y parasites with no amplification of Pfpm2 and Pfmdr1 genes. The median (IQR) IC50 of ferroquine for Pfk13 C580Y parasites with amplification of the Pfpm2 gene was 17.2 nM (14.5-20.5) versus 9.1 nM (7.9-10.7) for Pfk13 C580Y parasites with amplification of the Pfmdr1 gene.Conclusions: Ferroquine exerts promising efficacy against ACT-resistant isolates. Whereas Pfpm2 amplification was associated with the highest parasite tolerance to ferroquine, the susceptibility range observed was in accordance with those measured in ACT resistance-free areas. This enables consideration of ferroquine as a relevant therapeutic option against ACT-resistant malaria. [ABSTRACT FROM AUTHOR]- Published
- 2019
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- View/download PDF
16. Human peroxiredoxin 6 is essential for malaria parasites and provides a host-based drug target.
- Author
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Wagner, Matthias Paulus, Formaglio, Pauline, Gorgette, Olivier, Dziekan, Jerzy Michal, Huon, Christèle, Berneburg, Isabell, Rahlfs, Stefan, Barale, Jean-Christophe, Feinstein, Sheldon I., Fisher, Aron B., Ménard, Didier, Bozdech, Zbynek, Amino, Rogerio, Touqui, Lhousseine, and Chitnis, Chetan E.
- Abstract
The uptake and digestion of host hemoglobin by malaria parasites during blood-stage growth leads to significant oxidative damage of membrane lipids. Repair of lipid peroxidation damage is crucial for parasite survival. Here, we demonstrate that Plasmodium falciparum imports a host antioxidant enzyme, peroxiredoxin 6 (PRDX6), during hemoglobin uptake from the red blood cell cytosol. PRDX6 is a lipid-peroxidation repair enzyme with phospholipase A 2 (PLA 2) activity. Inhibition of PRDX6 with a PLA 2 inhibitor, Darapladib, increases lipid-peroxidation damage in the parasite and disrupts transport of hemoglobin-containing vesicles to the food vacuole, causing parasite death. Furthermore, inhibition of PRDX6 synergistically reduces the survival of artemisinin-resistant parasites following co-treatment of parasite cultures with artemisinin and Darapladib. Thus, PRDX6 is a host-derived drug target for development of antimalarial drugs that could help overcome artemisinin resistance. [Display omitted] • Human peroxiredoxin 6 (PRDX6) is internalized by P. falciparum blood stages • Inhibition of host PRDX6 causes lethal lipid-peroxidation damage in the parasite • Co-treatment with artemisinin and PRDX6 inhibitors overcomes artemisinin resistance • Targeting of a host enzyme like PRDX6 may prevent development of drug resistance Wagner et al. find that Plasmodium falciparum blood stages internalize human PRDX6, which repairs lipid-peroxidation damage. PRDX6 inhibitors prevent lipid repair and kill the parasite. Co-treatment of artemisinin-resistant strains with PRDX6 inhibitors and artemisinin restores susceptibility to artemisinin. PRDX6 thus provides a promising host-based target for anti-malaria drug development. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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17. Computational Design of Protein-Based Inhibitors of Plasmodium vivax Subtilisin-Like 1 Protease.
- Author
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Bastianelli, Giacomo, Bouillon, Anthony, Nguyen, Christophe, Le-Nguyen, Dung, Nilges, Michael, and Barale, Jean-Christophe
- Subjects
THERAPEUTIC use of proteins ,MALARIA treatment ,PLASMODIUM vivax ,SUBTILISINS ,ERYTHROCYTES - Abstract
Background: Malaria remains a major global health concern. The development of novel therapeutic strategies is critical to overcome the selection of multiresistant parasites. The subtilisin-like protease (SUB1) involved in the egress of daughter Plasmodium parasites from infected erythrocytes and in their subsequent invasion into fresh erythrocytes has emerged as an interesting new drug target. Findings: Using a computational approach based on homology modeling, protein–protein docking and mutation scoring, we designed protein–based inhibitors of Plasmodium vivax SUB1 (PvSUB1) and experimentally evaluated their inhibitory activity. The small peptidic trypsin inhibitor EETI-II was used as scaffold. We mutated residues at specific positions (P4 and P1) and calculated the change in free-energy of binding with PvSUB1. In agreement with our predictions, we identified a mutant of EETI-II (EETI-II-P4LP1W) with a Ki in the medium micromolar range. Conclusions: Despite the challenges related to the lack of an experimental structure of PvSUB1, the computational protocol we developed in this study led to the design of protein-based inhibitors of PvSUB1. The approach we describe in this paper, together with other examples, demonstrates the capabilities of computational procedures to accelerate and guide the design of novel proteins with interesting therapeutic applications. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
18. Cytometric measurement of in vitro inhibition of Plasmodium falciparum field isolates by drugs: a new approach for re-invasion inhibition study.
- Author
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Varela, Marie Louise, Razakandrainibe, Romy, Aldebert, Delphine, Barale, Jean Christophe, and Jambou, Ronan
- Abstract
Background: A flow cytometric method is proposed to study in vitro drug sensitivity of Plasmodium falciparum. Standard [
3 H]-hypoxanthine incorporation assay gives only information on inhibition of maturation by drugs. This method is usable on field isolates and provides data on both inhibition of maturation and re-invasion. Methods: The method is based on the staining of parasites with hydroethidine (HE) and thiazole orange (TO) which allow differential identification of early, trophozoite and late stage of the parasite by flow cytometry. Late stages of the parasites are obtained by incubation in culture for 24 hours. Reinvasion is followed by culturing parasitized red blood cells for 24 h more. Results: Compared to the standard [3 H]-hypoxanthine incorporation assay, it gave similar results as expressed by 50% inhibitory concentrations for chloroquine of laboratory strains and “field” isolates. The effect of quinine on the schizont-ring transition was also explored using this method. First data on the inhibition of re-invasion induced by quinine are presented for both P. falciparum-cultured strains and field isolates. Discussion: This method is simple to use event for field isolate study. It is suitable to analyse effect of drugs on steps of the parasite life cycle different for the maturation one. Using this method quinine was found to have a inhibitory effect on re-invasion of red cells by Plasmodium. [ABSTRACT FROM AUTHOR]- Published
- 2014
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19. Whole Pichia pastoris Yeast Expressing Measles Virus Nucleoprotein as a Production and Delivery System to Multimerize Plasmodium Antigens.
- Author
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Jacob, Daria, Ruffie, Claude, Dubois, Myriam, Combredet, Chantal, Amino, Rogerio, Formaglio, Pauline, Gorgette, Olivier, Pehau-Arnaudet, Gérard, Guery, Charline, Puijalon, Odile, Barale, Jean-Christophe, Ménard, Robert, Tangy, Frédéric, and Sala, Monica
- Subjects
PICHIA pastoris ,MEASLES virus ,NUCLEOPROTEINS ,PLASMODIUM ,LABORATORY mice ,PARASITIC disease treatment ,MEASLES vaccines - Abstract
Yeasts are largely used as bioreactors for vaccine production. Usually, antigens are produced in yeast then purified and mixed with adjuvants before immunization. However, the purification costs and the safety concerns recently raised by the use of new adjuvants argue for alternative strategies. To this end, the use of whole yeast as both production and delivery system appears attractive. Here, we evaluated Pichia pastoris yeast as an alternative vaccine production and delivery system for the circumsporozoite protein (CS) of Plasmodium, the etiologic agent of malaria. The CS protein from Plasmodium berghei (Pb) was selected given the availability of the stringent C57Bl/6 mouse model of infection by Pb sporozoites, allowing the evaluation of vaccine efficacy in vivo. PbCS was multimerized by fusion to the measles virus (MV) nucleoprotein (N) known to auto-assemble in yeast in large-size ribonucleoprotein rods (RNPs). Expressed in P. pastoris, the N-PbCS protein generated highly multimeric and heterogenic RNPs bearing PbCS on their surface. Electron microscopy and immunofluorescence analyses revealed the shape of these RNPs and their localization in peripheral cytoplasmic inclusions. Subcutaneous immunization of C57Bl/6 mice with heat-inactivated whole P. pastoris expressing N-PbCS RNPs provided significant reduction of parasitemia after intradermal challenge with a high dose of parasites. Thus, in the absence of accessory adjuvants, a very low amount of PbCS expressed in whole yeast significantly decreased clinical damages associated with Pb infection in a highly stringent challenge model, providing a proof of concept of the intrinsic adjuvancy of this vaccine strategy. In addition to PbCS multimerization, the N protein contributed by itself to parasitemia delay and long-term mice survival. In the future, mixtures of whole recombinant yeasts expressing relevant Plasmodium antigens would provide a multivalent formulation applicable for antigen combination screening and possibly for large-scale production, distribution and delivery of a malaria vaccine in developing countries. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
20. Computational Reverse-Engineering of a Spider-Venom Derived Peptide Active Against Plasmodium falciparum SUB1.
- Author
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Bastianelli, Giacomo, Bouillon, Anthony, Nguyen, Christophe, Crublet, Elodie, Pêtres, Stéphane, Gorgette, Olivier, Le-Nguyen, Dung, Barale, Jean-Christophe, and Nilges, Michael
- Subjects
SPIDER venom ,ANTI-infective agents ,PEPTIDES ,AMINO acid sequence ,PLASMODIUM falciparum ,MALARIA ,PARASITES ,ENZYME activation - Abstract
Background: Psalmopeotoxin I (PcFK1), a protein of 33 aminoacids derived from the venom of the spider Psalmopoeus Cambridgei, is able to inhibit the growth of Plasmodium falciparum malaria parasites with an IC
50 in the low micromolar range. PcFK1 was proposed to act as an ion channel inhibitor, although experimental validation of this mechanism is lacking. The surface loops of PcFK1 have some sequence similarity with the parasite protein sequences cleaved by PfSUB1, a subtilisin-like protease essential for egress of Plasmodium falciparum merozoites and invasion into erythrocytes. As PfSUB1 has emerged as an interesting drug target, we explored the hypothesis that PcFK1 targeted PfSUB1 enzymatic activity. Findings: Molecular modeling and docking calculations showed that one loop could interact with the binding site of PfSUB1. The calculated free energy of binding averaged 25.01 kcal/mol, corresponding to a predicted low-medium micromolar constant of inhibition. PcFK1 inhibited the enzymatic activity of the recombinant PfSUB1 enzyme and the in vitro P.falciparum culture in a range compatible with our bioinformatics analysis. Using contact analysis and free energy decomposition we propose that residues A14 and Q15 are important in the interaction with PfSUB1. Conclusions: Our computational reverse engineering supported the hypothesis that PcFK1 targeted PfSUB1, and this was confirmed by experimental evidence showing that PcFK1 inhibits PfSUB1 enzymatic activity. This outlines the usefulness of advanced bioinformatics tools to predict the function of a protein structure. The structural features of PcFK1 represent an interesting protein scaffold for future protein engineering. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
21. On the Knorr Synthesis of 6-Bromo-4-methylquinolin-2(1H)-one.
- Author
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Wlodarczyk, Nicolas, Simenel, Catherine, Delepierre, Muriel, Barale, Jean-Christophe, and Janin, Yves L.
- Subjects
MICROBIOLOGICAL synthesis ,COMMUNICABLE diseases ,QUINOLINE ,ANILIDES ,RING formation (Chemistry) ,ETHYL acetoacetate ,MAGNESIUM sulfate - Abstract
In the course of our work on infectious diseases, we were led to prepare 6-bromo-2-chloro-4-methylquinoline as a starting material. Since surprisingly little has been reported in the literature, the two synthetic steps to this compound were investigated. The synthesis involves a condensation between b-keto esters and 4-bromoaniline and the cyclization of the resulting anilides into 6-bromoquinolin- 2(1H)-one, otherwise known as the Knorr reaction. The
1 H NMR monitoring of the first step allowed us to optimize the conditions leading specifically to the anilide without the occurrence of the alternative crotonate. To illustrate the scope of our finding, few additional anilides featuring electron-attracting groups were prepared. The study of their cyclization revealed some unsuspected steric effect governing this second step. Aside from rectifying a few claims in this chemistry, this study led to a three-step preparation of 6-bromo-2-chloro-4-methylquinoline in 48% overall yield from 4- bromoaniline. [ABSTRACT FROM AUTHOR]- Published
- 2011
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22. CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use.
- Author
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Boeuf, Philippe, Vigan-Womas, Inès, Jublot, Delphine, Loizon, Séverine, Barale, Jean-Christophe, Akanmori, Bartholomew Dicky, Mercereau-Puijalon, Odile, and Behr, Charlotte
- Subjects
GENES ,CYTOKINES ,IMMUNOLOGY ,RNA ,IMMUNE response - Abstract
Background: Real-time PCR is becoming a common tool for detecting and quantifying expression profiling of selected genes. Cytokines mRNA quantification is widely used in immunological research to dissect the early steps of immune responses or pathophysiological pathways. It is also growing to be of clinical relevancy to immuno-monitoring and evaluation of the disease status of patients. The techniques currently used for "absolute quantification" of cytokine mRNA are based on a DNA standard curve and do not take into account the critical impact of RT efficiency. Results: To overcome this pitfall, we designed a strategy using external RNA as standard in the RT-PCR. Use of synthetic RNA standards, by comparison with the corresponding DNA standard, showed significant variations in the yield of retrotranscription depending the target amplified and the experiment. We then developed primers to be used under one single experimental condition for the specific amplification of human IL-1β, IL-4, IL-10, IL-12p40, IL-13, IL-15, IL-18, IFN-?, MIF, TGF-β1 and TNF-a mRNA. We showed that the beta-2 microglobulin (β2-MG) gene was suitable for data normalisation since the level of β2-MG transcripts in naïve PBMC varied less than 5 times between individuals and was not affected by LPS or PHA stimulation. The technique, we named CyProQuant-PCR (Cytokine Profiling Quantitative PCR) was validated using a kinetic measurement of cytokine transcripts under in vitro stimulation of human PBMC by lipopolysaccharide (LPS) or Staphylococcus aureus strain Cowan (SAC). Results obtained show that CyProQuant-PCR is powerful enough to precociously detect slight cytokine induction. Finally, having demonstrated the reproducibility of the method, it was applied to malaria patients and asymptomatic controls for the quantification of TGF-β1 transcripts and showed an increased capacity of cells from malaria patients to accumulate TGF-β1 mRNA in response to LPS. Conclusion: The real-time RT-PCR technique based on a RNA standard curve, CyProQuant-PCR, outlined here, allows fora genuine absolute quantification and a simultaneous analysis of a large panel of human cytokine mRNA. It represents a potent and attractive tool for immunomonitoring, lending itself readily to automation and with a high throughput. This opens the possibility of an easy and reliable cytokine profiling for clinical applications. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
23. Gene targeting demonstrates that the Plasmodium berghei subtilisin PbSUB2 is essential for red cell invasion and reveals spontaneous genetic recombination events.
- Author
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Uzureau, Pierrick, Barale, Jean-Christophe, Janse, Chris J., Waters, Andrew P., and Breton, Catherine Braun
- Subjects
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PROTEOLYTIC enzymes , *PARASITES , *PLASMODIUM , *SUBTILISINS - Abstract
The Plasmodium merozoite proteases involved in the crucial process of erythrocyte invasion are promising targets for novel malaria control strategies. We report here the characterization of the subtilisin-like protease SUB2 from the rodent parasites Plasmodium berghei and Plasmodium yoelii, leading the way to in vivo functional studies of this enzyme. The kinetics of expression and subcellular localization imply a central role for SUB2 in erythrocyte invasion. Through the use of gene targeting strategies, we assessed the relevance of P. berghei SUB2 for the intraerythrocytic cycle. The selection of recombinant Pbsub2-TrimycDuoXpress-tagged parasites and the proper expression of the modified coding region demonstrate that the Pbsub2 locus is accessible to genetic modifications. However, Pbsub2 knock-out parasites were not recovered, confirming the importance of PbSUB2 for P. berghei merozoite stages, and supporting the fact that its Plasmodium falciparum SUB2 orthologue is an attractive drug target candidate. Finally, we identify revertant parasites that have lost the integrated selection cassette while conserving a Pbsub2-tagged gene. These spontaneous reversion events should overcome the scarcity of selectable markers available for this parasite, giving access to multiple gene tagging strategies, which, together with the validation of a TrimycDuoXpress tag, would represent valuable new tools for studying the biology of P. berghei. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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24. The Mouse Homeoprotein mLIM-3 Is Expressed Early in Cells Derived from the Neuroepithelium and Persists in Adult Pituitary.
- Author
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SEIDAH, NABIL G., BARALE, JEAN-CHRISTOPHE, MARCINKIEWICZ, MIECZSLAW, MATTEI, MARIE-GENEVIÈVE, DAY, ROBERT, and CHRÉTIEN, MICHEL
- Published
- 1994
- Full Text
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25. Centromeric Plasmids and Artificial Chromosomes: New Kids on the Plasmodium Transfection Block.
- Author
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Barale, Jean-Christophe and Ménard, Robert
- Subjects
PLASMIDS ,ARTIFICIAL chromosomes ,GENE transfection ,PLASMODIUM ,HOSTS (Biology) ,BACTERIAL antigens ,BIOLOGICAL variation ,MICROBIAL genetics - Abstract
In this issue of Cell Host & Microbe, Iwanaga and colleagues () report on the construction of plasmids and artificial chromosomes that are stably maintained throughout the Plasmodium life cycle. These new tools will have multiple applications, from episome-based genetic strategies to studies on telomere biology and antigenic variation. [Copyright &y& Elsevier]
- Published
- 2010
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26. A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase.
- Author
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Giganti, David, Bouillon, Anthony, Tawk, Lina, Robert, Fabienne, Martinez, Mariano, Crublet, Elodie, Weber, Patrick, Girard-Blanc, Christine, Petres, Stéphane, Haouz, Ahmed, Hernandez, Jean-François, Mercereau-Puijalon, Odile, Alzari, Pedro M., and Barale, Jean-Christophe
- Published
- 2014
- Full Text
- View/download PDF
27. The LIM homeobox protein mLIM3/Lhx3 induces expression of the prolactin gene by a Pit-1/GHF-1-independent pathway in corticotroph AtT20 cells
- Author
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Girardin, Stephen E, Benjannet, Suzanne, Barale, Jean-Christophe, Chrétien, Michel, and Seidah, Nabil G
- Published
- 1998
- Full Text
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28. LANCL1, an erythrocyte protein recruited to the Maurer's clefts during Plasmodium falciparum development
- Author
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Blisnick, Thierry, Vincensini, Laetitia, Barale, Jean Christophe, Namane, Abdelkader, and Braun Breton, Catherine
- Subjects
- *
PLASMODIUM falciparum , *PROTOZOAN diseases , *MEMBRANE proteins , *ERYTHROCYTES - Abstract
Abstract: As the malarial parasite Plasmodium falciparum develops inside the erythrocyte, parasite-derived membrane structures, referred to as Maurer''s clefts, play an important role in parasite development by delivering parasite proteins to the host cell surface, and participating in the assembly of the cytoadherence complex, essential for the pathogenesis of cerebral malaria. PfSBP1 is an integral membrane protein of the clefts, interacting with an erythrocyte cytosolic protein, identified here as the human Lantibiotic synthetase component C-like protein LANCL1. LANCL1 is specifically recruited to the surface of Maurer''s clefts in P. falciparum mature blood stages. We propose that the interaction between PfSBP1 and LANCL1 is central for late steps of the parasite development to prevent premature rupture of the red blood cell membrane. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
29. A Key Role for Plasmodium Subtilisin-like SUB1 Protease in Egress of Malaria Parasites from Host Hepatocytes.
- Author
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Tawk, Lina, Lacroix, Céline, Gueirard, Pascale, Kent, Robyn, Gorgette, Olivier, Thiberge, Sabine, Mercereau-Puijalon, Odile, Ménard, Robert, and Barale, Jean-Christophe
- Subjects
- *
PLASMODIUM , *PLASMODIIDAE , *PROTEOLYTIC enzymes , *HYDROLASES , *MALARIA - Abstract
In their mammalian host, Plasmodium parasites have two obligatory intracellular development phases, first in hepatocytes and subsequently in erythrocytes. Both involve an orchestrated process of invasion into and egress from host cells. The Plasmodium SUB1 protease plays a dual role at the blood stage by enabling egress of the progeny merozoites from the infected erythrocyte and priming merozoites for subsequent erythrocyte invasion. Here, using conditional mutagenesis in P. berghei, we show that SUB1 plays an essential role at the hepatic stage. Stage-specific sub1 invalidation during prehepatocytic development showed that SUB1-deficient parasites failed to rupture the parasitophorous vacuole membrane and to egress from hepatocytes. Furthermore, mechanically released parasites were not adequately primed and failed to establish a blood stage infection in vivo. The critical involvement of SUB1 in both pre-erythrocytic and erythrocytic developmental phases qualifies SUB1 as an attractive multistage target for prophylactic and therapeutic anti-Plasmodium intervention strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
30. In Silico Screening on the Three-dimensional Model of the Plasmodium vivax SUB1 Protease Leads to the Validation of a Novel Anti-parasite Compound.
- Author
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Bouillon, Anthony, Giganti, David, Benedet, Christophe, Gorgette, Olivier, Pêtres, Stéphane, Crublet, Elodie, Girard-Blanc, Christine, Witkowski, Benoit, Ménard, Didier, Nilges, Michael, Mercereau-Puijalon, Odile, Stoven, Véronique, and Barale, Jean-Christophe
- Subjects
- *
DRUG resistance in microorganisms , *PLASMODIUM falciparum , *MALARIA , *SERINE proteinases , *ERYTHROCYTES , *MEROZOITES - Abstract
Widespread drug resistance calls for the urgent development of new antimalarials that target novel steps in the life cycle of Plasmodium falciparum and Plasmodium vivax. The essential subtilisin-like serine protease SUB1 of Plasmodium merozoites plays a dual role in egress from and invasion into host erythrocytes. It belongs to a new generation of attractive drug targets against which specific potent inhibitors are actively searched. Wecharacterize here the P. vivax SUB1 enzyme and show that it displays a typical auto-processing pattern and apical localization in P. vivax merozoites. To search for small PvSUB1 inhibitors, we took advantage of the similarity of SUB1 with bacterial subtilisins and generated P. vivax SUB1 three-dimensional models. The structure-based virtual screening of a large commercial chemical compounds library identified 306 virtual best hits, of which 37 were experimentally confirmed inhibitors and 5 had Ki values of <50μM for PvSUB1. Interestingly, they belong to different chemical families. The most promising competitive inhibitor of PvSUB1(compound 2) was equally active on PfSUB1 and displayed anti-P. falciparum and Plasmodium berghei activity in vitro and in vivo, respectively. Compound 2 inhibited the endogenous PfSUB1 as illustrated by the inhibited maturation of its natural substrate PfSERA5 and inhibited parasite egress and subsequent erythrocyte invasion. These data indicate that the strategy of in silico screening of three-dimensional models to select for virtual inhibitors combined with stringent biological validation successfully identified several inhibitors of the PvSUB1 enzyme. The most promising hit proved to be a potent cross-inhibitor of PlasmodiumSUB1, laying the groundwork for the development of a globally active small compound antimalarial. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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