8 results on '"BACCIU, DONATELLA"'
Search Results
2. ICEVchInd5 is prevalent in epidemic Vibrio cholerae O1 El Tor strains isolated in India
- Author
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Ceccarelli, Daniela, Spagnoletti, Matteo, Bacciu, Donatella, Danin-Poleg, Yael, Mendiratta, Deepak K., Kashi, Yechezkel, Cappuccinelli, Piero, Burrus, Vincent, and Colombo, Mauro M.
- Published
- 2011
- Full Text
- View/download PDF
3. Transposition of the heat-stable toxin astA gene into a Gifsy-2-related prophage of Salmonella enterica serovar Abortusovis
- Author
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Bacciu, Donatella, Falchi, Giovanni, Spazziani, Alessandra, Bossi, Lionello, Marogna, Gavino, Leori, Guido Sisinnio, Rubino, Salvatore, and Uzzau, Sergio
- Subjects
Salmonella -- Research ,Virulence (Microbiology) -- Research ,Bacterial transformation -- Research ,Genetic transformation -- Research ,Biological sciences - Abstract
The horizontal transfer and acquisition of virulence genes via mobile genetic elements have been a major driving force in the evolution of Salmonella pathogenicity. Serovars of Salmonella enterica carry variable assortments of phage-encoded virulence genes, suggesting that temperate phages play a pivotal role in this process. Epidemic isolates of S. enterica serovar Typhimurium are consistently lysogenic for two lambdoid phages, Gifsy-1 and Gifsy-2, carrying known virulence genes. Other serovars of S. enterica, including serovars Dublin, Gallinarum, Enteritidis, and Hadar, carry distinct prophages with similarity to the Gifsy phages. In this study, we analyzed Gifsy-related loci from S. enterica serovar Abortusovis, a pathogen associated exclusively with ovine infection. A cryptic prophage, closely related to serovar Typhimurium phage Gifsy-2, was identified. This element, named Gifsy-2AO, was shown to contribute to serovar Abortusovis systemic infection in lambs. Sequence analysis of the prophage b region showed a large deletion which covers genes encoding phage tail fiber proteins and putative virulence factors, including type III secreted effector protein SseI (GtgB, SrfH). This deletion was identified in most of the serovar Abortusovis isolates tested and might be dependent on the replicative transposition of an adjacent insertion sequence, IS1414, previously identified in pathogenic Escherichia coli strains. IS1414 encodes heat-stable toxin EAST1 (astA) and showed multiple genomic copies in isolates of serovar Abortusovis. To our knowledge, this is the first evidence of intergeneric transfer of virulence genes via insertion sequence elements in Salmonella. The acquisition of IS1414 (EAST1) and its frequent transposition within the chromosome might improve the fitness of serovar Abortusovis within its narrow ecological niche.
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- 2004
4. New V. cholerae atypical El Tor variant emerged during the 2006 epidemic outbreak in Angola
- Author
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Cappuccinelli Piero, Bacciu Donatella, Spagnoletti Matteo, Ceccarelli Daniela, and Colombo Mauro M
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Microbiology ,QR1-502 - Abstract
Abstract Background V. cholerae is the etiological agent of cholera, a major public health concern in most developing countries. Virulence of V. cholerae relies on the powerful cholera toxin, encoded by the CTX prophage. The emergence of new pathogenic variants in the recent years has been mostly associated with new CTX prophage rearrangements. Results In this retrospective study, we show that the epidemic V. cholerae O1 El Tor strain responsible for the 2006 outbreak in Angola is clonally and genetically different from El Tor strains circulating in the 1990s in the same area. Strains from 2006 carry ICEVchAng3 of the SXT/R391 family. This ICE is associated with a narrower multidrug resistance profile compared to the one conferred by plasmid p3iANG to strains of the 1990s. The CTX prophage carried by 2006 El Tor strains is characterized by rstRET and ctxBCla alleles organized in a RS1-RS2-Core array on chromosome I. Interestingly, the newly emerging atypical strain belongs to a clade previously known to comprise only clinical isolates from the Indian subcontinent that also contain the same ICE of the SXT/R391 family. Conclusions Our findings remark the appearance of a novel V. cholerae epidemic variant in Africa with a new CTXΦ arrangement previously described only in the Indian Subcontinent.
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- 2011
- Full Text
- View/download PDF
5. Expression of ompR gene in the acid adaptation and thermal resistance of Salmonella Enteritidis SE86.
- Author
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Ritter, Ana Carolina, Bacciu, Donatella, Santi, Lucélia, Rubino, Salvatore, Uzzau, Sergio, and Tondo, Eduardo César
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GENE expression , *THERMAL resistance , *IMMUNOBLOTTING , *SALMONELLA enteritidis , *FOODBORNE diseases - Abstract
Introduction: The objective of this study was to evaluate the involvement of the ompR gene in the acid adaptation and thermal resistance of S. Enteritidis SE86, responsible agent of more than 95 % of investigated food-borne diseases, throughout the last decade in Southern Brazil. In this study, we constructed a mutant strain of S. Enteritidis SE86 (ΔompR) that was attenuated by a knockout technique. The OmpR protein expression was determined in a tagged (3XFLAG) strain of S. Enteritidis SE86. Methodology: The mutant strains were cultivated separately in nutrient broth and nutrient broth supplemented with 1% glucose (NBG) to induce acid adapted cells. The organisms were exposed to different temperature such as 37 °C, 52 °C, and 60 °C. The survival of the SE86 wild type (WT) and attenuated strain was determined by bacterial count, and the tagged protein (ompR::3XFLAG cat::FLAG) was detected by SDS-PAGE and immunoblotting with anti-FLAG antibodies Results: Results showed that when exposed at 52°C, the acid-adapted SE86 WT cells were completely inactivated after 300 minutes; however, non-adapted cells (WT and ΔompR) and acid-adapted ΔompR demonstrated higher thermal sensitivity, since they were completely inactivated in 240 minutes. At 60°C, the acid-adapted SE86 ΔompR also demonstrated higher sensitivity that SE86 WT, being totally inactivated after 15 minutes, while the WT cells were inactivated in 20 minutes. Conclusion: The acid adapted cells showed increased expression of OmpR when exposed to 52°C and 60°C, this confirmed the requirement of acid adaptation for S. Enteritidis SE86 to resist elevated temperatures. [ABSTRACT FROM AUTHOR]
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- 2014
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- View/download PDF
6. Investigation of rpoS and dps Genes in Sodium Hypochlorite Resistance of Salmonella Enteritidis SE86 Isolated from Foodborne Illness Outbreaks in Southern Brazil.
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RITTER, ANA CAROLINA, BACCIU, DONATELLA, SANTI, LUCÉLIA, BEYS DA SILVA, WALTER ORLANDO, VAINSTEIN, MARILENE HENNING, RUBINO, SALVATORE, UZZAU, SERGIO, and TONDO, EDUARDO CESAR
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SALMONELLA enteritidis , *SODIUM hypochlorite , *FOODBORNE diseases , *GENES , *OXIDATIVE stress , *PROTEINS - Abstract
In Rio Grande do Sul, southern Brazil, Salmonella Enteritidis is one of the principal microorganisms responsible for foodborne disease. The present study was conducted to compare the sodium hypochlorite resistance of Salmonella Enteritidis SE86 with that of other strains of Salmonella Enteritidis isolated from different regions of the world and to investigate the involvement of the rpoS and dps genes in resistance to this disinfectant. We tested five Salmonella Enteritidis wild-type (WT) strains isolated from different countries, two mutant strains of Salmonella Enteritidis SE86, and two tagged (3XFLAG) strains of Salmonella Enteritidis SE86 for their resistance to sodium hypochlorite (200 ppm). The survival of the WT and attenuated strains was determined based on bacterial counts, and tagged proteins (Dps and RpoS) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with anti-FLAG antibodies. None of the WT strains of Salmonella Enteritidis were totally inactivated after 20 min. The SE86 strain lacking dps was more sensitive to sodium hypochlorite than was the WT SE86 strain, with a 2-log reduction in counts after 1 min. The RpoS and Dps proteins were actively expressed under the conditions tested, indicating that in Salmonella Enteritidis SE86 these genes, which are expressed when in contact with sodium hypochlorite, are related to oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
7. New V. cholerae atypical El Tor variant emerged during the 2006 epidemic outbreak in Angola.
- Author
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Ceccarelli, Daniela, Spagnoletti, Matteo, Bacciu, Donatella, Cappuccinelli, Piero, and Colombo, Mauro M.
- Subjects
CHOLERA ,EPIDEMICS ,VIBRIO infections ,DEVELOPING countries - Abstract
Background: V. cholerae is the etiological agent of cholera, a major public health concern in most developing countries. Virulence of V. cholerae relies on the powerful cholera toxin, encoded by the CTX prophage. The emergence of new pathogenic variants in the recent years has been mostly associated with new CTX prophage rearrangements. Results: In this retrospective study, we show that the epidemic V. cholerae O1 El Tor strain responsible for the 2006 outbreak in Angola is clonally and genetically different from El Tor strains circulating in the 1990s in the same area. Strains from 2006 carry ICEVchAng3 of the SXT/R391 family. This ICE is associated with a narrower multidrug resistance profile compared to the one conferred by plasmid p3iANG to strains of the 1990s. The CTX prophage carried by 2006 El Tor strains is characterized by rstR
ET and ctxBCla alleles organized in a RS1-RS2-Core array on chromosome I. Interestingly, the newly emerging atypical strain belongs to a clade previously known to comprise only clinical isolates from the Indian subcontinent that also contain the same ICE of the SXT/R391 family. Conclusions: Our findings remark the appearance of a novel V. cholerae epidemic variant in Africa with a new CTXϕ arrangement previously described only in the Indian Subcontinent. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
8. Molecular characterization of ICE VchVie0 and its disappearance in Vibrio cholerae O1 strains isolated in 2003 in Vietnam.
- Author
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Bani, Stefania, Mastromarino, Patrizia Nina, Ceccarelli, Daniela, Le Van, An, Salvia, Anna Maria, Ngo Viet, Quynh Tram, Hai, Duong Huynh, Bacciu, Donatella, Cappuccinelli, Piero, and Colombo, Mauro Maria
- Subjects
VIBRIO cholerae ,DRUG resistance ,AMOXICILLIN ,AMINOGLYCOSIDES ,ERYTHROMYCIN ,EPIDEMICS - Abstract
We analyzed 28 epidemic Vibrio cholerae O1 strains isolated in the region of Thua Thien Hue (Vietnam) in 2003. Ubiquitous amoxicillin, prevalent aminoglycosides and sporadic erythromycin resistances were observed. All were devoid of plasmids, class 1 integrons and ICEs and showed the same BglI ribotype, irrespective of their site of isolation and resistance pattern. A strain isolated in 1990 in the same area was resistant to amoxicillin and aminoglycosides but characterized by a different ribotype. This strain contained ICE VchVie0, belonging to the SXT/R391 ICE family, devoid of any resistance cluster. The molecular analysis of three conserved and six variable regions outlined an original genetic profile. ICEs not coding for resistance to drugs seem to be more frequent than supposed, and this finding reinforces the idea that the SXT/R391 family of genetic elements is wide and composite. The clearance of ICE VchVie0 in the 2003 epidemic may be explained by the lack of any resistance determinant as a favorable selective marker. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
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