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Gait is impaired in musculoskeletal conditions, such as knee arthropathy. Gait analysis is used in clinical practice to inform diagnosis and monitor disease progression or intervention response. However, clinical gait analysis relies on subjective visual observation of walking as objective gait analysis has not been possible within clinical settings due to the expensive equipment, large-scale facilities, and highly trained staff required. Relatively low-cost wearable digital insoles may offer a solution to these challenges. In this work, we demonstrate how a digital insole measuring osteoarthritis-specific gait signatures yields similar results to the clinical gait-lab standard. To achieve this, we constructed a machine learning model, trained on force plate data collected in participants with knee arthropathy and controls. This model was highly predictive of force plate data from a validation set (area under the receiver operating characteristics curve [auROC] = 0.86; area under the precision-recall curve [auPR] = 0.90) and of a separate, independent digital insole dataset containing control and knee osteoarthritis subjects (auROC = 0.83; auPR = 0.86). After showing that digital insole-derived gait characteristics are comparable to traditional gait measurements, we next showed that a single stride of raw sensor time-series data could be accurately assigned to each subject, highlighting that individuals using digital insoles can be identified by their gait characteristics. This work provides a framework for a promising alternative to traditional clinical gait analysis methods, adds to the growing body of knowledge regarding wearable technology analytical pipelines, and supports clinical development of at-home gait assessments, with the potential to improve the ease, frequency, and depth of patient monitoring.

Quantifying viral growth rates is key to understanding evolutionary dynamics and the potential for mutants to escape antiviral drugs. Defining evolutionary escape paths and their impact on viral fitness allows for the development of drugs that are resistant to escape. In the case of HIV, combination antiretroviral therapy can successfully prevent or treat infection, but it relies on strict adherence to prevent escape. Here, we present a method termed QuickFit that enables the quantification of viral fitness by employing large numbers of parallel viral cultures to measure growth rates accurately. QuickFit consistently recapitulated HIV growth measurements obtained by traditional approaches, but with significantly higher throughput and lower rates of error. This method represents a promising tool for rapid and consistent evaluation of viral fitness.

Quantifying viral growth rates is key to understanding evolutionary dynamics and the potential for mutants to escape antiviral drugs. Defining evolutionary escape paths and their impact on viral fitness allows for the development of drugs that are resistant to escape. In the case of HIV, combination antiretroviral therapy can successfully prevent or treat infection, but it relies on strict adherence to prevent escape. Here, we present a method termed QuickFit that enables the quantification of viral fitness by employing large numbers of parallel viral cultures to measure growth rates accurately. QuickFit consistently recapitulated HIV growth measurements obtained by traditional approaches, but with significantly higher throughput and lower rates of error. This method represents a promising tool for rapid and consistent evaluation of viral fitness. [ABSTRACT FROM AUTHOR]

Leafhoppers comprise over 20,000 plant‐sap feeding species, many of which are important agricultural pests. Most species rely on two ancestral bacterial symbionts, Sulcia and Nasuia, for essential nutrition lacking in their phloem and xylem plant sap diets. To understand how pest leafhopper genomes evolve and are shaped by microbial symbioses, we completed a chromosomal‐level assembly of the aster leafhopper's genome (ALF; Macrosteles quadrilineatus). We compared ALF's genome to three other pest leafhoppers, Nephotettix cincticeps, Homalodisca vitripennis, and Empoasca onukii, which have distinct ecologies and symbiotic relationships. Despite diverging ~155 million years ago, leafhoppers have high levels of chromosomal synteny and gene family conservation. Conserved genes include those involved in plant chemical detoxification, resistance to various insecticides, and defence against environmental stress. Positive selection acting upon these genes further points to ongoing adaptive evolution in response to agricultural environments. In relation to leafhoppers' general dependence on symbionts, species that retain the ancestral symbiont, Sulcia, displayed gene enrichment of metabolic processes in their genomes. Leafhoppers with both Sulcia and its ancient partner, Nasuia, showed genomic enrichment in genes related to microbial population regulation and immune responses. Finally, horizontally transferred genes (HTGs) associated with symbiont support of Sulcia and Nasuia are only observed in leafhoppers that maintain symbionts. In contrast, HTGs involved in non‐symbiotic functions are conserved across all species. The high‐quality ALF genome provides deep insights into how host ecology and symbioses shape genome evolution and a wealth of genetic resources for pest control targets. [ABSTRACT FROM AUTHOR]

Genomes of aphids (family Aphididae) show several unusual evolutionary patterns. In particular, within the XO sex determination system of aphids, the X chromosome exhibits a lower rate of interchromosomal rearrangements, fewer highly expressed genes, and faster evolution at nonsynonymous sites compared with the autosomes. In contrast, other hemipteran lineages have similar rates of interchromosomal rearrangement for autosomes and X chromosomes. One possible explanation for these differences is the aphid's life cycle of cyclical parthenogenesis, where multiple asexual generations alternate with 1 sexual generation. If true, we should see similar features in the genomes of Phylloxeridae, an outgroup of aphids which also undergoes cyclical parthenogenesis. To investigate this, we generated a chromosome-level assembly for the grape phylloxera, an agriculturally important species of Phylloxeridae, and identified its single X chromosome. We then performed synteny analysis using the phylloxerid genome and 30 high-quality genomes of aphids and other hemipteran species. Unexpectedly, we found that the phylloxera does not share aphids' patterns of chromosome evolution. By estimating interchromosomal rearrangement rates on an absolute time scale, we found that rates are elevated for aphid autosomes compared with their X chromosomes, but this pattern does not extend to the phylloxera branch. Potentially, the conservation of X chromosome gene content is due to selection on XO males that appear in the sexual generation. We also examined gene duplication patterns across Hemiptera and uncovered horizontal gene transfer events contributing to phylloxera evolution. [ABSTRACT FROM AUTHOR]

Dominance breaking is a powerful technique in improving the solving efficiency of Constraint Optimization Problems (COPs) by removing provably suboptimal solutions with additional constraints. While dominance breaking is effective in a range of practical problems, it is usually problem specific and requires human insights into problem structures to come up with correct dominance breaking constraints. Recently, a framework is proposed to generate nogood constraints automatically for dominance breaking, which formulates nogood generation as solving auxiliary Constraint Satisfaction Problems (CSPs). However, the framework uses a pattern matching approach to synthesize the auxiliary generation CSPs from the specific forms of objectives and constraints in target COPs, and is only applicable to a limited class of COPs. This paper proposes a novel rewriting system to derive constraints for the auxiliary generation CSPs automatically from COPs with nested function calls, significantly generalizing the original framework. In particular, the rewriting system exploits functional constraints flattened from nested functions in a high-level modeling language. To generate more effective dominance breaking nogoods and derive more relaxed constraints in generation CSPs, we further characterize how to extend the system with rewriting rules exploiting function properties, such as monotonicity, commutativity, and associativity, for specific functional constraints. Experimentation shows significant runtime speedup using the dominance breaking nogoods generated by our proposed method. Studying patterns of generated nogoods also demonstrates that our proposal can reveal dominance relations in the literature and discover new dominance relations on problems with ineffective or no known dominance breaking constraints. [ABSTRACT FROM AUTHOR]

Background The small hive beetle (SHB), Aethina tumida , has emerged as a worldwide threat to honey bees in the past two decades. These beetles harvest nest resources, feed on larval bees, and ultimately spoil nest resources with gelatinous slime together with the fungal symbiont Kodamaea ohmeri. Results Here, we present the first chromosome-level genome assembly for the SHB. With a 99.1% representation of conserved (BUSCO) arthropod genes, this resource enables the study of chemosensory, digestive, and detoxification traits critical for SHB success and possible control. We use this annotated assembly to characterize features of SHB sex chromosomes and a female-skewed primary sex ratio. We also found chromosome fusion and a lower recombination rate in sex chromosomes than in autosomes. Conclusions Genome-enabled insights will clarify the traits that allowed this beetle to exploit hive resources successfully and will be critical for determining the causes of observed sex ratio asymmetries. [ABSTRACT FROM AUTHOR]

Evolutionary innovations generate phenotypic and species diversity. Elucidating the genomic processes underlying such innovations is central to understanding biodiversity. In this study, we addressed the genomic basis of evolutionary novelties in the glassy-winged sharpshooter (Homalodisca vitripennis , GWSS), an agricultural pest. Prominent evolutionary innovations in leafhoppers include brochosomes, proteinaceous structures that are excreted and used to coat the body, and obligate symbiotic associations with two bacterial types that reside within cytoplasm of distinctive cell types. Using PacBio long-read sequencing and Dovetail Omni-C technology, we generated a chromosome-level genome assembly for the GWSS and then validated the assembly using flow cytometry and karyotyping. Additional transcriptomic and proteomic data were used to identify novel genes that underlie brochosome production. We found that brochosome-associated genes include novel gene families that have diversified through tandem duplications. We also identified the locations of genes involved in interactions with bacterial symbionts. Ancestors of the GWSS acquired bacterial genes through horizontal gene transfer (HGT), and these genes appear to contribute to symbiont support. Using a phylogenomics approach, we inferred HGT sources and timing. We found that some HGT events date to the common ancestor of the hemipteran suborder Auchenorrhyncha, representing some of the oldest known examples of HGT in animals. Overall, we show that evolutionary novelties in leafhoppers are generated by the combination of acquiring novel genes, produced both de novo and through tandem duplication, acquiring new symbiotic associations that enable use of novel diets and niches, and recruiting foreign genes to support symbionts and enhance herbivory. [ABSTRACT FROM AUTHOR]

Amino acids, the building blocks of proteins in the cells and tissues, are of fundamental importance for cell survival, maintenance, and proliferation. The liver plays a critical role in amino acid metabolism and detoxication of byproducts such as ammonia. Urea cycle disorders with hyperammonemia remain difficult to treat and eventually necessitate liver transplantation. In this study, ornithine transcarbamylase deficient (Otcspf‐ash) mouse model was used to test whether knockdown of a key glutamine metabolism enzyme glutaminase 2 (GLS2, gene name: Gls2) or glutamate dehydrogenase 1 (GLUD1, gene name: Glud1) could rescue the hyperammonemia and associated lethality induced by a high protein diet. We found that reduced hepatic expression of Gls2 but not Glud1 by AAV8‐mediated delivery of a short hairpin RNA in Otcspf‐ash mice diminished hyperammonemia and reduced lethality. Knockdown of Gls2 but not Glud1 in Otcspf‐ash mice exhibited reduced body weight loss and increased plasma glutamine concentration. These data suggest that Gls2 hepatic knockdown could potentially help alleviate risk for hyperammonemia and other clinical manifestations of patients suffering from defects in the urea cycle. [ABSTRACT FROM AUTHOR]

Social responsibility (SR) is becoming an increasingly significant component of many firms' strategic planning decisions. Research has shown that consumers tend to reward socially responsible behavior. However, there has been little testing of the construct in the hospitality industry. Additionally, when other important variables that influence consumer brand loyalty are considered, will brand social responsibility image (BSRI) still play a significant role? This study investigates the importance of SR and its impact on brand loyalty, relative to product quality and service quality in the quick-service restaurant industry. The authors were also interested to learn whether BSRI impacted consumers' image of product and service quality. It was found that BSRI had a positive impact on brand loyalty, product quality, and service quality. However, product quality was a significantly stronger predictor of brand loyalty than BSRI. Where the vast majority of studies of SR have utilized scenario analysis of hypothetical firms, this study utilizes consumers' perceptions of a real-world firm. [ABSTRACT FROM AUTHOR]

An exploratory study was conducted to examine the impact of product quality and service quality on attitude- and behavior-based brand loyalty for quick-service restaurants. A total of 175 respondents were surveyed. The results showed that quick-service restaurants need to he more concerned with product quality especially in taste, freshness, and temperature, and focus on their overall service quality to build brand loyalty. Moreover, the results from correlation tests show that brand loyalty for one brand may affect brand loyalty towards another brand. The restaurants considered were McDonald's, Burger King and Wendy's. [ABSTRACT FROM AUTHOR]

Helen Day and colleagues report on the positive outcomes that have resulted from the implementation of a clinical model for delivering high dependency care for children and young people. [ABSTRACT FROM AUTHOR]

Examines the role of honor in the international states system. Definition of honor; Importance of honor in political philosophy; 'Word of honor' and courtly society; Nationalization of king's honor; Honor and interest as rhetoric of British foreign policy.

In his book, The Rise and Fall of Strategic Planning, Henry Mintzberg proposed that planning and implementation must not be two separate functions, but a continuous managerial decision-making process. One that is based first, on an astute analysis of volatile environmental market conditions, and second, on management's ability to effectively transform its offering to satisfy this moving target. This article questions the use of the concept and term strategic management in restaurants to describe what may be best termed strategic market management. Additionally, a framework within which modern restaurant planning takes place is presented. [ABSTRACT FROM PUBLISHER]

As the environment has become more dynamic, management has been forced to compete at consistently higher levels. One area where management and researchers have apparently not maximized their intellectual capital is on the topic of positioning. Positioning is determining how the firm wants to be viewed relative to major competitors. The primary focus of existing efforts on positioning has been the use of positioning/perceptual maps to determine a firm’s multiattribute/multidimensional position. Although these maps do provide quality data, they ignore the fact that consumers also holistically rank competing firms on a linear or hierarchical continuum. The objectives of this article include proposing a typology and a methodology for measuring a firm’s linear market position. In addition, the author desires to begin a dialogue that would lead to a valid, reliable, and commonly used means of measuring a firm’s position. [ABSTRACT FROM PUBLISHER]

The ALLEGRO gravitational wave detector has been in nearly continuous operation for the past four years with a duty cycle of over 90%. The data is continuously filtered for bursts and has a noise level near 12 mK, or 8 × 10[sup -22] strain/Hz. An initial data exchange under the IGEC has been completed. [ABSTRACT FROM AUTHOR]

Making effective strategic decisions is not a simple task. Though no heuristic can remove potential planning hazards or absolve management of the responsibility for guiding the firm, good information can improve the results. The strategic analysis questioning sequence provides management with an organized, systematic process with which to: locate key situational factors; determine their importance; develop optional strategies; and finally to assess the viability of potential strategies. As the process is ingrained in the firm's planning process and each manager's mental (cognitive) model, better decisions should follow. [ABSTRACT FROM PUBLISHER]

Hierarchical clustering methods are presented using a simplified example. The article describes and explains six steps in the cluster analysis process: variable selection, data management, selection of clustering methods, deriving cluster solutions, validating results, and interpreting results. The article concludes by giving the reader an example of how each of the steps was processed in the identification and description of three market segments based on entertainment preferences. The purpose of the article is to encourage increased usage of this valuable means of revealing market niches by researchers in the field of hospitality. [ABSTRACT FROM PUBLISHER]

Focuses on intellectual property under the North American Free Trade Agreement. Implementation of a tripartite strategy for the protection of intellectual property; Definition of intellectual property; Options for treating foreign rightholders; Intellectual property and the International Court of Justice.

The purpose of this article is to show how strategic planning can assist in the implementation of nutritionally sound strategies and policies, based on facts and organized thinking rather than on a decision-maker's presumption. A nutritionally related strategic plan for a foodservice facility will be presented to demonstrate the required decisions and process. Additionally, the majority of the information needed for the plan's situational analysis can be integrated into an on-going management information system, shortening and simplfying the process for future plans, and hastening the recognition of potential opportunities and threats. [ABSTRACT FROM PUBLISHER]