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Nitrogen fixation by bacteria is essential for sustaining the growth, development, and yield of legumes. Pot experiments were carried out at the International Institute of Tropical Agriculture (IITA) in the glasshouse between August to December 2018/2019 cropping season in Ibadan, Nigeria. Field studies were also performed in two different agroecological zones, “Ibadan and Ikenne” between August and December of 2019/2020 cropping season. The studies were set up to determine the potential of nitrogen-fixation bacteria strain inoculation on the nitrogen-fixation potential of 10 Bambara groundnut (BGN) genotypes, namely, TVSu-378, TVSu-506, TVSu-787, TVSu-1,606, TVSu-1,698, TVSu-1739, TVSu-710, TVSu-365, TVSu-475, and TVSu-305. The strains were inoculated as a broth to seedlings of each BGN genotype in the pot experiment. While six seeds from each BGN genotype were coated with each of the following nitrogen-fixation bacteria (Bradyrhizobium japonicum strains), FA3, USDA110, IRJ2180A, and RACA6, nitrogen fertilizer (urea, 20 kg/ha) was applied as a check to the nitrogen-fixation bacteria to seedlings of BGN genotypes 2 weeks after planting in both glasshouses and fields. Uninoculated plants served as controls (zero inoculation and zero fertilization). The field experiments were arranged in Randomized Complete Block Design (RCBD), while the glasshouse experiments were arranged in Complete Randomized Design (CRD) in triplicate. The result gotten showed that higher nodule numbers and weight were recorded in TVSu-1739 and TVSu-475 in both locations and seasons compared to other genotypes; the highest nitrogen fixed values were recorded among BGN genotypes TVSu-1739, TVSu-1,698, TVSu-787, TVSu-365, TVSu-305, TVSu-710, and TVSu-1,606, with a range of (62–67 kg ha−1), and were mostly enhanced by RACA6 and USDA110 strains compared to other strains that were used.

This study seeks to increase the efficiency of yam breeding practice using farmers’ insight at the trait and socioeconomic levels. A three-staged multisampling procedure was employed and 792 yam farmers from four geopolitical zones, comprising 10 states and the Federal Capital Territory, Abuja in Nigeria were randomly selected. Farmer’s preference criteria and factors pertinent to improving the efficiency of yam breeding in Nigeria were documented. The data obtained were analyzed using a 5-point Likert scale to identify major traits farmers consider in the yam cultivar selection decision. Kendall’s coefficient of concordance was used to measure the degree of agreement of ranking among the farmers. Factors influencing farmers’ trait preference for yam cultivars were evaluated using a multinomial-ordered logistic regression model. The result revealed that yam varieties with high germination rates, disease-free quality, big tuber sizes, early maturity, and good pounding attributes are held in high esteem. The most critical constraint limiting the production of yam in the study area includes pest and disease attack, climate change, high cost of seed yam, high cost of staking, and weed infestation. Sex, age, access to credit, membership to yam association, total land owned, and years of experience as a yam farmer significantly influence farmers’ ability to select yam cultivars with preferred attributes. A strategic effort needs to be given to these farmers’ desired yam attributes and factored into developing improved yam varieties for increased adoption and enhanced food security in Nigeria.

Abstract Yam (Dioscorea spp.) is a staple crop for millions of people in the tropics and subtropics. Its genetic improvement through breeding is being challenged by pre-zygotic and post-zygotic cross-compatibility barriers within and among species. Studies dissecting hybridization barriers on yam for improving the crossability rates are limited. This study aimed to assess the cross-compatibility, which yielded fruit set, viable seeds and progeny plants in an extensive intraspecific and interspecific crossing combinations in a yam genetic improvement effort to understand the internal and exogenous factors influencing pollination success. Cross-compatability was analyzed at the individual genotype or family level using historical data from crossing blocks and seedling nurseries from 2010 to 2020 at the International Institute of Tropical Agriculture (IITA). The average crossability rate (ACR) was lower in interspecific crossing combinations (6.1%) than intraspecific ones (27.6%). The seed production efficiency (SPE) values were 1.1 and 9.3% for interspecific and intraspecific crosses, respectively. Weather conditions and pollinator's skills are the main contributors to the low success rate in the intraspecific cross combinations in yam breeding. At the same time, genetic distance and heterozygosity played little role. Interspecific cross barriers were both pre-zygotic and post-zygotic, resulting from the evolutionary divergence among the yam species. Dioscorea rotundata had higher interspecific cross-compatibility indices than D. alata. Distant parents produced intraspecific crossbred seeds with higher germination rates compared to closest parents (r = 0.21, p = 0.033). This work provided important insights into interspecific and intraspecific cross-compatibility in yam and suggested actions for improving hybridization practices in yam breeding programs.

IntroductionLandraces represent a significant gene pool of African cultivated white Guinea yam diversity. They could, therefore, serve as a potential donor of important traits such as resilience to stresses as well as food quality attributes that may be useful in modern yam breeding. This study assessed the pattern of genetic variability, quantitative trait loci (QTLs), alleles, and genetic merits of landraces, which could be exploited in breeding for more sustainable yam production in Africa.MethodsA total of 86 white Guinea yam landraces representing the popular landraces in Nigeria alongside 16 elite clones were used for this study. The yam landraces were genotyped using 4,819 DArTseq SNP markers and profiled using key productivity and food quality traits.Results and discussionGenetic population structure through admixture and hierarchical clustering methods revealed the presence of three major genetic groups. Genome-wide association scan identified thirteen SNP markers associated with five key traits, suggesting that landraces constitute a source of valuable genes for productivity and food quality traits. Further dissection of their genetic merits in yam breeding using the Genomic Prediction of Cross Performance (GPCP) allowed identifying several landraces with high crossing merit for multiple traits. Thirteen landraces were identified as potential genitors to develop segregating progenies to improve multiple traits simultaneously for desired gains in yam breeding. Results of this study provide valuable insights into the patterns and the merits of local genetic diversity which can be utilized for identifying desirable genes and alleles of interest in yam breeding for Africa.

Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine cultivated for its starchy underground or aerial tubers in the tropics and subtropics. It is an allogamous and polyploid species that reproduces by both sexual and asexual mechanisms. However, many of the landrace cultivars, including most of the popular varieties, reproduce exclusively by vegetative propagation (planting the tubers). These varieties are either sterile or produce sparse and irregular flowering with high flower abortion rate, low fruit and seed set. Production of crossbreed seeds for genetic improvement and for maintaining genetic diversity in yams is, therefore, mainly achieved through natural or managed pollination. Flowering in yam is mostly dioecious and, in some instances, monoecious. Flowering asynchrony, sticky nature of the pollen grains, and cross incompatibility are among the challenges in making genetic progress in yam breeding. There are many limitations in basic and applied knowledge of yam flower biology and pollination. This paper, therefore, reviews the flowering biology, pollination, and methods of improving pollination efficiency in yam breeding programs.

Yam (Dioscorea spp.) is a nutritional and medicinal staple tuber crop grown in the tropics and sub-tropics. Among the food yam species, water yam (Dioscorea alata L.) is the most widely distributed and cultivated species worldwide. Tuber dry matter content (DMC) and oxidative browning (OxB) are important quality attributes that determine cultivar acceptability in water yam. This study used a single nucleotide polymorphism (SNP) assay from a diversity arrays technology (DArT) platform for a genome-wide association study (GWAS) of the two quality traits in a panel of 100 water yam clones grown in three environments. The marker–trait association analysis identified significant SNPs associated with tuber DMC on chromosomes 6 and 19 and with OxB on chromosome 5. The significant SNPs cumulatively explained 45.87 and 12.74% of the total phenotypic variation for the tuber DMC and OxB, respectively. Gene annotation for the significant SNP loci identified important genes associated in the process of the proteolytic modification of carbohydrates in the dry matter accumulation pathway as well as fatty acid β-oxidation in peroxisome for enzymatic oxidation. Additional putative genes were also identified in the peak SNP sites for both tuber dry matter and enzymatic oxidation with unknown functions. The results of this study provide valuable insight for further dissection of the genetic architecture of tuber dry matter and enzymatic oxidation in water yam. They also highlight SNP variants and genes useful for genomics-informed selection decisions in the breeding process for improving food quality traits in water yam.