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A redescription of Corydoras undulatus Regan, 1912 is presented. The original description of C. undulatus is very succinct, as is its diagnosis, which is based only on external morphology. Additional information in the scientific literature on this species is scarce. Specimens from the distribution area of this species were analyzed; Paraná and Paraguay river basins in Argentina, Uruguay river basin in Argentina, Brazil and Uruguay, and the Laguna dos Patos system in Brazil. Morphological analysis, principal component analysis (PCA), meristic comparison and osteological description were performed. Corydoras undulatus can be distinguished from its congeners mainly by having the following combination of characters: mesethmoid short, with anterior tip short, smaller than 50% of the entire bone length; posterior margin of the pectoral-fin spine with nearly all serrations directed towards origin of spine; pectoral-fin spine with conical serrations; and its peculiar color pattern. The analysis of the material from the different basins did not indicate relevant morphological differences, suggesting that the species presents a wide distribution in La Plata and Laguna dos Patos drainages. The shared geographic distribution between these two systems is also present in other fish species. The current work presents data about the type locality, taxonomy, osteology, distribution and ontogenetic variation of color pattern in C. undulatus. Comments on the identity of a very similar congener, Corydoras latus, will also be provided.

This study has explored the valence of sibship that may empower the self-esteem of children with asthma at the interpersonal, environmental control competence, emotionality management, and body-image levels. It has been assumed that the relationship between siblings may have a moderating effect on the negative impact that asthma has on child’s development. Seventy children suffering from chronic asthma have been involved: 40 children with siblings (experimental group) and 30 sibling-free children (control group). The children with asthma have exhibited higher levels of self-esteem in comparison with the sibling-free children. The results of the study, at the clinical significance level, highlight how meaningful could be the involvement of healthy siblings to support the development, and to ease the compliance of children suffering from asthma. The outcomes have confirmed the supportive valence of sibship for the self-esteem of the children with asthma.

Crude palm oil extraction is one of the sources of livelihood in Ghana's Central Region. However, the water use and wastewater generation associated with the industry have not been given adequate attention. This study assessed the water consumption and wastewater generation by small-scale crude palm oil extraction mills in the region. Twenty-five (25) mills were selected from four palm oil-processing local government areas in the region. An interview guide was used to obtain information about the operations of the mills and corroborated through structured observations. Water consumption and wastewater generation were measured using a graduated plastic bucket. Water for processing was found to be sourced from hand-dug wells (56%), treated piped water (20%), boreholes with handpumps (16%) and rivers (8%). Water was bought at US$1.93 (boreholes) and US$2.89 (piped water) per cubic metre. The recurrent cost of water was the same as that paid for domestic use. For a litre of palm oil produced, 0.760-2.391 litres of water were consumed and 68-82% returned as wastewater. Sixty-eight percent of the water was used for boiling. The distance to water source influenced the consumption, with higher water consumption recorded for mills with on-plot water sources. Higher recurrent costs of water did not necessarily lead to lower water consumption. There was no significant difference in the water consumption and wastewater production between the wet and dry processing methods. The small-scale palm oil processing industry requires attention to manage a potential competition between commercial and domestic water use in rural and small towns. HIGHLIGHTS 1 L of crude palm oil produced could consume more than twice that volume of water.; Wastewater return factor is 62–89%.; Boiling of fruits consumes 68% of the water and generates 62% of the wastewater.; 10 L of palm oil produced consumes the equivalent of one person's water demand in rural Ghana.; Higher recurrent costs of water did not necessarily lead to lower water consumption.;

Water scarcity is a global issue confronting the present generation, particularly acute in arid regions such as Palestine. Treated wastewater reuse is recognized as a strategic solution. The challenge in obtaining effluent quality that meets the reuse requirements of the area is how to select the best treatment technology. A multi-criteria decision-making method is necessary for assessing the sustainability of different wastewater treatment technologies. This study selected the most sustainable secondary treatment technology that could produce quality effluent for agricultural reuse at the Rafah wastewater treatment plant in the Gaza Strip. The Evidential Reasoning method was applied to evaluate the sustainability of sand filters, constructed wetlands, activated sludge, and bio-tower technologies. The evaluation was based on four sustainability dimensions and thirty-five indicators. The dimensions and indicators were obtained from a review of related literature and validated by experts. Using a questionnaire, the judgment of local experts (professionals working on the project, selected university professors, and members of the agricultural NGOs) was used to evaluate the dimensions and indicators qualitatively. Data analysis was done using the Intelligent Decision System and Expert Choice software tools. The utility interval-based evidential reasoning ranking technique was used to rank the wastewater treatment options with and without ignorance as follows: constructed wetlands > sand filters > bio-towers > activated sludge. Constructed wetlands ranked as the most sustainable alternative, with a minimum utility of 0.7345. The environmental dimension, with a relative weighting of 60.4%, was the dimension with the greatest influence on ranking the secondary wastewater treatment alternatives.

La economía circular y la bioeconomía han resultado ser una alternativa a nivel mundial a los modelos de producción lineales y basados en combustibles fósiles y materias primas muchas veces no–renovables. Aunque en Centroamérica existen iniciativas de este tipo, principalmente en el sector bioenergético, se debe impulsar la aplicación de estos principios en sectores más amplios como la bio emediación, la fabricación de bio–fertilizantes e incluso de materiales de más alto valor como bio–polímeros y bio–cosméticos. De igual manera, la preservación de la biodiversidad debe ser un objetivo común centroamericano, aunado a esto, se debe añadir el efecto sinérgico entre los países centroamericanos, en donde el SICA puede jugar un rol institucional neutro, más no pasivo, de coordinación y articulación entre los países. La migración hacia una economía bio–basada y circular con enfoque ambientalmente sostenible es viable en la región considerando las tecnologías actuales y el potencial de biodiversidad de Centroamérica.

With a term now entered into common language, the historical period we are experiencing is defined as "Anthropocene", to underline the serious responsibility of human activities for the global impacts produced and for the quality of life on our planet. At the same time, we are in an era of transition, characterized by a necessary transformation of society, which, starting from the current economic model, based on the logic of consumption of resources, production of waste and pollution and energy dependence from fossil fuels, we are moving towards a new, more sustainable future characterized by the search for solutions to multiple environmental criticalities and strategies for adapting to their now irreversible effects. Numerous examples of responsible citizenship and bottom up initiatives, such as student living labs, demonstrate the importance of active participation, a more aware knowledge of sustainability issues that lead to the adoption of effective initiatives on a local scale for the health of our cities.

In response to the important environmental impacts produced by the road transport network during all its life cycle stages, a set of measurements to include sustainable principles have been identified between 1997–2021, analysed and mapped by applying SciMAT and VOSviewer. The results have shown the predominance of issues which analyse the life cycle assessment of road infrastructures in its both environmental and economic dimension as a way to mitigate their effects on climate change, including the reduction of resources and energy consumption, or of energy consumption and greenhouse gases emissions during the construction and operation stages, respectively. However, the incorporation of the social life cycle assessment dimension, their adaptation to climate change, and the use of smart roads are still in an early stage. Therefore, efforts to include these issues are necessary to address their design according to the resilience concept and ensuring a cleaner and more competitive and climate-neutral Europe.

Malignant pleural mesothelioma is an aggressive malignancy with poor prognosis. Unilateral pleural effusion is frequently the initial clinical sign requiring therapeutic thoracentesis, which also offers a diagnostic opportunity. Detection of soluble biomarkers can support diagnosis, but few show good diagnostic accuracy. Here, we studied the expression levels and discriminative power of two putative biomarkers, prosaposin and extracellular sulfatase SULF-1, identified by proteomic and transcriptomic analysis, respectively. Pleural effusions from a total of 44 patients (23 with mesothelioma, 8 with lung cancer, and 13 with non-malignant disease) were analyzed for prosaposin and SULF-1 by enzyme-linked immunosorbent assay. Pleural effusions from mesothelioma patients had significantly higher levels of prosaposin and SULF-1 than those from non-malignant disease patients. Receiver-operating characteristic (ROC) analysis showed that both biomarkers have good discriminating power as pointed out by an AUC value of 0.853 (p = 0.0005) and 0.898 (p < 0.0001) for prosaposin and SULF-1, respectively. Combining data ensued a model predicting improvement of the diagnostic performance (AUC = 0.916, p < 0.0001). In contrast, prosaposin couldn’t discriminate mesothelioma patients from lung cancer patients while ROC analysis of SULF-1 data produced an AUC value of 0.821 (p = 0.0077) but with low sensitivity. In conclusion, prosaposin and SULF-1 levels determined in pleural effusion may be promising biomarkers for differential diagnosis between mesothelioma and non-malignant pleural disease. Instead, more patients need to be enrolled before granting the possible usefulness of these soluble proteins in differentiating mesothelioma pleural effusions from those linked to lung cancer.

A study on water/energy balances at the household scale is performed using Life Cycle Assessment (LCA) to estimate Greenhouse Gas (GHG) emissions and impacts resulting from multiple scenarios incorporating various options for: (i) component sizing, (ii) energy usage, and (iii) water reuse. Sustainability indicators are evaluated to select feasible options, while reducing whole life cycle GHG emissions. Water reuse schemes using rainwater are strongly dependent on rainfall availability and require significant tank volumes. Schemes using only gray water are more compact but more energy for treatment is needed before usage. Schemes obtained by combining both options perform better in terms of reliability and sustainability.

RESUMO: Trata-se de uma revisão integrativa da literatura, em que foi realizada uma vasta pesquisa nos bancos de dados do The Scientific Electronic Library Online - SciELO e na Biblioteca Digital Brasileira de Teses e Dissertações, com objetivo geral de analisar as produções que abordam ou correlacionam a inserção de pessoas com deficiência visual (PCDV) no mercado de trabalho brasileiro, publicados no período de 2002 a 2018. A partir dos critérios estabelecidos, foram selecionados 15 (quinze) pesquisas para a amostra final entre artigos, tese e dissertações que contribuíram de forma expressiva, atendendo ao objetivo principal desta revisão integrativa. A partir dos resultados, as discussões foram organizadas em discutir o conceito de deficiência, caracterização da deficiência visual, acesso ao mercado de trabalho, lei de cotas e centros de reabilitação. Os resultados analisados revelam expressivamente que é significativa a falta de inclusão de pessoas com deficiência visual no mercado de trabalho nos últimos 16 anos, apontando as dificuldades e oportunidades dos últimos anos, seja por falta de estudo, capacitação, adaptação ao novo ambiente laboral ou inclusão por lei de cotas. PALAVRAS-CHAVE: Inclusão, Deficiência Visual, Mercado de Trabalho.

The level of One Health (OH), or “One Health-ness,” of health interventions has been defined as the capacity to operate according to six dimensions concerning OH operations and OH infrastructures, respectively (thinking, planning, and working; and information sharing, reciprocal learning, and systemic organization). Although health initiatives and research increasingly claim their orientation toward OH, such a capacity is rarely assessed. The objective of this study is to evaluate the One Health-ness of the academic team of the University of Bologna (UNIBO Team) working in the “ELEPHANT” project (Empowering universities' Learning and rEsearch caPacities in the one Health Approach for the maNagement of animals at the wildlife, livestock and human interface in SouTh Africa). This project involves universities, six from South Africa and two from Europe, and aims at embedding OH in research and learning to enable the control of diseases at the human, animal, and environmental interface, and to emphasize the interests of local African communities with wildlife conservation. The methodology adopts the NEOH method, developed in 2018 by the EU-COST Action, “Network for the Evaluation of One Health.” The approach is based on questionnaires delivered to participants, which focus on the six OH dimensions, and then translate answers into quantitative metrics through the OH Index (OHI) and the OH Ratio (OHR). The following two evaluation levels are foreseen: the whole project and the single partner institutions. The evaluations are carried on in parallel, with preliminary, mid-term, and final assessments, to monitor the efficacy of the project actions. The preliminary evaluation of the UNIBO Team resulted in the OHI of 0.23 and the OHR of 1.69 which indicate a low degree of OH-ness and an imbalance between OH operation and OH infrastructure. The UNIBO case study will be the baseline for the evaluation of the other partner institutions involved in the ELEPHANT project. This type of evaluation can support the implementation of OH practices inside a project and underpin the strategies that allow to achieving more effective results. Any improvement in the OH-ness of each single academic team can be also considered as a result of the ELEPHANT project, thus showing its multiplier effect in the context.

Introduction: Following an outbreak of meningococcal epidemic in 2015 and 2016 in Tuscany, we registered a higher demand for antimeningococcal vaccination (anti-Men ACWY) by Healthcare Workers of the University Hospital of Pisa [Azienda Ospedaliero Universitaria Pisana (AOUP)]. The purpose of this work is to analyze and discuss data on vaccination coverage resulting from this vaccination campaign.Materials and Methods: We conducted a monocentric study about anti-Men vaccination in the healthcare workers of the AOUP following the outbreak of meningococcal meningitis that occurred mainly in the population of the Tuscan provinces of Pisa, Pistoia, Prato, and Florence. The variables under examination were age, sex, educational qualification, and job profile. Department healthcare workers were vaccinated with two types of conjugated tetravalent vaccines for the A, C, Y, and W135 strains. Data were analyzed using the SPSS software.Results: The total population of the workers in AOUP was 7,188 subjects; the population considered in the study was 5,889. Between 2015 and 2017, a total of 2,423 subjects (41.1%) underwent anti-Men vaccination. Women, older HCWs, those with a lower educational qualification, doctors, and the HCWs of the maternal and child department, and imaging department recorded a statistically significant better vaccine compliance.Discussion: The AOUP, implementing the program of the Tuscany Region of vaccination against Neisseria meningitidis, has contributed to reduce the incidence of invasive meningococcal disease. Some critical issues remain in the compliance of some sections of the population, despite the high level of adherence recorded in this case, probably also due to the great media coverage of the event.

The concept of sustainability in the road construction sector is a complex issue because of the various steps that contribute to the production and release of greenhouse gas (GHG) emissions. Addressing this issue, the European Commission has put various policy initiatives in place to encourage the construction industry to adopt circular economy (CE) and industrial symbiosis (IS) principles e.g., the use of recycled materials. Cooperativa Trasporti Imola (CTI), a company located in the Emilia-Romagna region (Italy), has been chosen for the current case study to examine practices, management, and the industrial symbiosis network among various companies in the road construction and rehabilitation sector. In this regard, the use of steel slags, obtained by an electric arc furnace (EAF), and reclaimed asphalt pavement (RAP), obtained by the deconstruction and milling of old asphalt pavement have been investigated. Two mixtures of recycled hot Mix Asphalt (HMA) i) were prepared incorporating different recycled material percentages for the wearing and binder course, respectively, ii) were characterized in terms of size distribution, strength modulus and volumetric properties, iii) and finally were compared to the performances of two mixtures entirely designed by virgin materials for the wearing and binder course, respectively. Therefore, the Life Cycle Assessment (LCA) tool was chosen to evaluate the environmental impacts that affect the designed road life cycle. The results show that recycling RAP and EAF slags in a CTI batch plant provides benefits by reducing the consumption of virgin bitumen and aggregates and by reducing CO2eq emissions. Finally, practical implications on the use of recycled materials in new asphalt mixtures from a life cycle and industrial symbiosis perspective are provided.

In this study, empty palm fruit bunch fibre was used for solids-liquid separation of palm oil mill wastewater and the sludge was sun-dried for potential uses as soil conditioner or solid fuel. The fibre was manually compressed into a wooden mould to obtain a fibre bulk density of 70 ​kg/m3. A composite wastewater sample was poured evenly over the surface of the fibre. The wet solids were sun-dried for 14 days to achieve a moisture content of 99% solids and up to 65% COD removal. Analysis of the dried sludge showed nutrients content (% of dry weight) of 0.84 for total nitrogen, 0.15 for phosphorus and 0.49 for potassium. The mean calorific value of the dried sludge was 17.1 ​MJ/kg. The results show the potential of sun-dried palm oil mill wastewater sludge for use as sustainable soil conditioner or solid fuel. The effluent from the solids-liquid separation must be given additional treatment as it may still contain harmful constituents.

Soluble mesothelin-related peptide (SMRP) is a promising biomarker for malignant pleural mesothelioma (MPM), but several confounding factors can reduce SMRP-based test’s accuracy. The identification of these confounders could improve the diagnostic performance of SMRP. In this study, we evaluated the sequence of 1,000 base pairs encompassing the minimal promoter region of the MSLN gene to identify expression quantitative trait loci (eQTL) that can affect SMRP. We assessed the association between four MSLN promoter variants and SMRP levels in a cohort of 72 MPM and 677 non-MPM subjects, and we carried out in vitro assays to investigate their functional role. Our results show that rs2235503 is an eQTL for MSLN associated with increased levels of SMRP in non-MPM subjects. Furthermore, we show that this polymorphic site affects the accuracy of SMRP, highlighting the importance of evaluating the individual’s genetic background and giving novel insights to refine SMRP specificity as a diagnostic biomarker.

Malignant pleural mesothelioma (MPM) is a cancer mainly caused by asbestos fiber inhalation, characterized by an extremely long latency and poor prognosis. Recently, researchers have focused on testing the diagnostic ability of several biomarkers. Gamma-Glutamyltransferase (GGT) has been demonstrated to be the sum of several GGT sub-fractions activity, classified based on their molecular weight in big-GGT, medium-GGT, small-GGT, and free-GGT. This work aims to evaluate whether specific GGT fractional enzymatic activity patterns could be helpful in MPM diagnosis. We analyzed blood samples from 175 workers previously exposed to asbestos, 157 non-exposed healthy subjects, and 37 MPM patients through a molecular exclusion chromatographic method. We found a specific profile of GGT fractions activity, significantly associated with MPM, resulting in an increase in b-, m- activity, along with an evident, yet not significant, decrease in f-activity. Receiver-operating characteristic (ROC) analysis showed that the best Area Under Curve (AUC) value resulted from the combined index b/f (0.679, 95% CI: 0.582–0.777). Combining the b-/f-GGT activity with the levels of serum mesothelin-related protein (SMRP; another promising MPM biomarker) improved the diagnostic accuracy, increasing the AUC value to 0.875 (95% CI: 0.807–0.943, p =

OBJECTIVES: Despite an asbestos ban in the European Union, exposure to asbestos still represents an occupational risk. Biomarkers of DNA damage and genomic instability in groups exposed to asbestos may contribute to the identification of subgroups/subjects at higher risk. METHODS: A cross-sectional study was conducted on 468 male individuals (80 working in occupational settings with potential exposure to asbestos fibers, 202 retired workers with past exposure, and 186 non-exposed controls) to compare genomic instability, cell proliferation and differentiation level using the non-invasive micronucleus buccal cytome assay. Data on demographic variables, lifestyle, and occupational history were collected with a standardized questionnaire. Micronuclei (MN) and other biomarkers of DNA damage and genomic instability were scored in a minimum of 2000/1000 cells per individual, respectively. RESULTS: Univariate and multivariate analysis showed opposite associations of MN frequency with current and former exposure. Compared to unexposed controls, workers with current potential exposure to asbestos had 55% lower MN frequency [95% confidence interval (CI) 71–29%, P

Abstract Malignant pleural mesothelioma (MPM) is an aggressive human cancer and miRNAs can play a key role for this disease. In order to broaden the knowledge in this field, the miRNA expression was investigated in a large series of MPM to discover new pathways helpful in diagnosis, prognosis and therapy. We employed nanoString nCounter system for miRNA profiling on 105 MPM samples and 10 healthy pleura. The analysis was followed by the validation of the most significantly deregulated miRNAs by RT-qPCR in an independent sample set. We identified 63 miRNAs deregulated in a statistically significant way. MiR-185, miR-197, and miR-299 were confirmed differentially expressed, after validation study. In addition, the results of the microarray analysis corroborated previous findings concerning miR-15b-5p, miR-126-3p, and miR-145-5p. Kaplan-Meier curves were used to explore the association between miRNA expression and overall survival (OS) and identified a 2-miRNA prognostic signature (Let-7c-5p and miR-151a-5p) related to hypoxia and energy metabolism respectively. In silico analyses with DIANA-microT-CDS highlighted 5 putative targets in common between two miRNAs. With the present work we showed that the pattern of miRNAs expression is highly deregulated in MPM and that a 2-miRNA signature can be a new useful tool for prognosis in MPM.

Abstract Introduction Decreased plasma BDNF (pBDNF) levels have been proposed as a biomarker in illness phases of mood disorders. Serum cortisol (seC) levels are an index of energy mobilization and stress. The aim of this cross‐sectional study was to evaluate pBDNF and seC levels in workers exposed to occupational stress and suffering from Adjustment Disorders (AD) compared to healthy workers. Methods Plasma BDNF and seC levels were measured by means of specific immunoassays in 64 AD patients and 38 healthy controls. Perceived and occupational stress was evaluated in patients and controls using the Psychological Stress Measure (PSM) and the Job Content Questionnaire (JCQ). Psychopatological symptoms in patients were assessed using specific rating scales. Results Plasma BDNF levels resulted significantly higher in patients than in controls, whereas no significant differences were found for seC levels. In patients but not in controls pBDNF levels showed a significant positive correlation with seC levels. Perceived stress levels were positively correlated with all psychopatological rating scales scores. Conclusions BDNF could play a key role in the pathophysiology of stress‐related disorders and its peripheral levels elevation could contribute to protect neurons under stress. Further research is needed focusing on biomarkers for stress‐related disorders as a potential tool for the diagnosis and prevention of occupational diseases.

The trade-off between awareness and interruption is a crucial aspect in network fault notifiers: Low severity alarms should not distract operators from other primary tasks, however it might be crucial that operators promptly react to critical notifications. A notification system should hence determine when a particular interruption is appropriate and how it should be presented. In this direction, this paper presents a multistep design path beginning from the objective of designing a proof-of-concept for a glanceable alarm notification component for telecommunication network management systems based on a peripheral display approach. In particular the goal was a notifier guided by severity-based strategies and offering the information expressiveness of a one-notification-at-the-time perspective while enriching it with overview capabilities to guarantee (possibly subliminal) long-term local and global content comprehension and prompt reaction only when the interruption from the foreground task is dictated by the fault severity. A first design macro-phase led to the simple yet effective GLANCE (GLanceable Alarm Notification for a User Centered Experience) model, based on a visual coding technique oriented to comprehension and reaction, and a transition strategy oriented to interruptions and reaction. A second design macro-phase studied the application of GLANCE to a personal customizable multichannel notification tool and to a service-oriented fault monitor for digital terrestrial television broadcasting networks.

Malignant pleural mesothelioma (MPM) is a rare cancer originated from pleural mesothelial cells. MPM has been associated with long-term exposure to asbestos. In this work we performed a comparative proteomic analysis of biphasic pleural mesothelioma (B-PM). Tissue biopsies were obtained from 61 patients who were subjected to a diagnostic thoracoscopy. 2D/MS based approach was used for proteomic analysis. The 22 proteins found differentially expressed in B-PM, with respect to benign, were analyzed by Ingenuity Pathways Analysis and compared with those obtained for epitheliod pleural mesothelioma (E-PM). A different activation of transcription factors, proteins and cytokines were observed between two subtypes.

Asbestos fibers include a group of silicate minerals that occur in the environment and are widely employed in occupational settings. Asbestos exposure has been associated to various chronic diseases; such as pulmonary fibrosis; mesothelioma; and lung cancer; often characterized by a long period of latency. Underlying mechanisms that are behind the carcinogenic effect of asbestos have not been fully clarified. Therefore; we have conducted an epidemiological study to evaluate the relationship between 8-hydroxy-2′-deoxyguanosine (8-oxodG), one of the most reliable biomarkers of oxidative stress and oxidative DNA damage; and asbestos exposure in the peripheral blood of residents in Tuscany and Liguria regions; Italy; stratified by occupational exposure to this carcinogen. Levels of 8-oxodG were expressed such as relative adduct labeling (RAL); the frequency of 8-oxodG per 105 deoxyguanosine was significantly higher among exposed workers with respect to the controls; i.e., 3.0 ± 0.2 Standard Error (SE) in asbestos workers versus a value of 1.3 ± 0.1 (SE) in unexposed controls (p < 0.001). When the relationship with occupational history was investigated; significant higher levels of 8-oxodG were measured in current and former asbestos workers vs. healthy controls; 3.1 ± 0.3 (SE) and 2.9 ± 0.2 (SE), respectively. After stratification for occupational history; a significant 194% excess of adducts was found in workers with 10 or more years of past asbestos exposure (p < 0.001). 8-oxodG can be used for medical surveillance programs of cohorts of workers with past and recent exposures to carcinogens for the identification of subjects requiring a more intense clinical surveillance.

O objetivo deste estudo foi analisar o processo de formação de terapeutas ocupacionais de uma Universidade pública da região Nordeste do Brasil, na perspectiva da educação inclusiva. Para tanto, utilizou-se uma abordagem qualitativa de pesquisa. O instrumento utilizado para coletar os dados foi o roteiro de entrevista, que permitiu entrevistar de forma reflexiva 14 terapeutas ocupacionais. A análise de dados empregada foi a de conteúdo, especificamente, a temática. Os resultados indicaram que os(as) terapeutas ocupacionais são favoráveis à educação inclusiva, porém suas formações profissionais estavam direcionadas para uma dimensão clínica-terapêutica. Mediante essa situação, foram sugeridas mudanças: incentivar a interface entre as áreas da saúde e educação; analisar/reformular a matriz curricular do curso; associar o conteúdo da Terapia Ocupacional no campo da educação inclusiva com os eixos do ensino, pesquisa e extensão. Diante disso, é necessário que a Universidade pesquisada programe e execute ações que contribuam para formação profissional do(a) terapeuta ocupacional na perspectiva da educação inclusiva.

[This corrects the article DOI: 10.1371/journal.pone.0085935.].

European Commission is strongly committed into issues related to plastic materials production and plastic waste management. While the Circular Economy Package has set targets generally referred to recycling rates, the European Strategy for plastics in a circular economy (and related action plan), fosters sustainability along the entire plastic value chain: from primary producers to converters, brand owners and retailers to waste collectors and recyclers. The Directive on the reduction of the impact of certain plastic products on the environment (more commonly known as Directive on Single-Use-Plastics, waiting for publication in the Official Journal of the European Union) rules targets on ten plastic products most often found as littering on global beaches, directly affecting plastic industry and, consequently, market. Policy makers and industrial stakeholders are called upon to collaborate. The article aims to illustrate interactions between European Commission and all plastic value chain stakeholders on implementing measures to reach ambitious targets pursued by the recent European policy. The study shows how European Commission has robustly worked to regulate production and consumption patterns on plastic carrier bags and packaging (including food packaging) thus facilitating the achievement of specific targets provided by the recent Directive. However, additional provisions concerning market restriction have been introduced; industrial stakeholders carried on a prompt response by promoting the creation of alliances, join venture and association, as well as a more integrated plastic value chain. On the base of this purpose, a virtuous example of a closed supply chain is presented.

O objetivo deste estudo foi analisar o processo de formação de terapeutas ocupacionais de uma universidade pública da Região Nordeste do Brasil, na perspectiva da educação inclusiva. Para tanto, utilizou-se uma abordagem qualitativa de pesquisa. O instrumento utilizado para coletar os dados foi o roteiro de entrevista, que permitiu entrevistar de forma relexiva 14 terapeutas ocupacionais. A análise de dados empregada foi a de conteúdo, especiicamente a temática. Os resultados indicaram que os(as) terapeutas ocupacionais são favoráveis à educação inclusiva, porém suas formações proissionais estavam direcionadas para uma dimensão clínica-terapêutica. Mediante essa situação, os(as) terapeutas ocupacionais sugerem algumas mudanças: incentivar a interface entre as áreas da saúde e educação; analisar/reformular a matriz curricular do curso; associar o conteúdo da Terapia Ocupacional no campo da educação inclusiva com os eixos do ensino, pesquisa e extensão. Diante disso, é necessário que a Universidade pesquisada programe e execute ações que contribuam para formação proissional do(a) terapeuta ocupacional na perspectiva da educação inclusiva.

Indoor air quality (IAQ) management in public spaces is assuming a remarkable importance. Busy environments, like airport terminals, are currently regarded as possible hotspots and IAQ is a crucial element for passengers and staff protection, as well as a key aspect of airport passenger experience. A one-month monitoring period has been performed on IAQ in the airport of Bologna (Italy), as prototypal example of large regional airport. Four strategic areas within the airport have been equipped with electronic monitoring platforms, including different contaminants and two microclimatic sensors. Data suggest that daily variation in IAQ parameters typically follow the activity pattern of the different environments under study (i.e., passengers’ flows) for gaseous contaminants, where particulate matter counts oscillate in a definite range, with a significant role played by ventilation system. Gaseous contaminants show a correlation between indoor and outdoor concentrations, mainly due to airside activities. Micro-climatic comfort parameters have been tested to match with standards for commercial environments. As results appears in line with typical households IAQ values, the current air ventilation system appears to be adequate. Nevertheless, an integrated air management system, based on real-time monitoring, would lead to optimization and improvement in environmental and economical sustainability.

BackgroundAsbestos-induced mesothelial inflammatory processes are thought to be the basic mechanisms underlying Malignant Mesothelioma (MM) development. Detection of MM often occurs at late stage due to the long and unpredictable latent period and the low incidence in asbestos exposed individuals. The aim of this study was to investigate early immunological biomarkers to characterize the prognostic profile of a possible asbestos-induced disease, in subjects from a MM hyperendemic area.MethodsThe Luminex Multiplex Panel Technology was used for the simultaneous measurement of serum levels of a large panel of 47 analytes, including cytokines and growth factors, from workers previously exposed to asbestos (Asb-workers), asbestos-induced MM patients and healthy subjects. In addition, to explore the influence on serum cytokines profile exerted by SV40 infection, a cofactor in MM development, a quantitative real time PCR was performed for sequences detection in the N-terminal and intronic regions of the SV40 Tag gene. Statistical analysis was done by means of the Mann-Whitney test and the Kruskall-Wallis test for variance analysis.ResultsA variety of 25 cytokines linked to pulmonary inflammation and tumor development were found significantly associated with Asb-workers and MM patients compared with healthy controls. A specific pattern of cytokines were found highly expressed in Asb-workers: IFN-alpha (pConclusionThis study shows that, in subjects from an hyperendemic area for MM, the C-C chemokine RANTES is associated with the exposure to asbestos fibres. If validated in larger samples, this factor could have the potential to be a critical biomarker for MM prognosis as recently reported for breast tumor.

Genes involved in the carcinogenetic mechanisms underlying malignant pleural mesothelioma (MPM) are still poorly characterized. So far, mesothelin (MSLN) has aroused the most interest. It encodes for a membrane glycoprotein, frequently over-expressed in various malignancies such as MPM, and ovarian and pancreatic cancers. It has been proposed as a diagnostic and immunotherapeutic target with promising results. However, an alternative therapeutic approach seems to rise, whereby synthetic molecules, such as antisense oligonucleotides, could be used to inhibit MSLN activity. To date, such a gene-level inhibition has been attempted in two studies only, both on pancreatic and ovarian carcinoma cell lines, with the use of silencing RNA approaches. With regard to MPM, only one cell line (H2373) has been employed to study the effects of MSLN depletion. Indeed, the knowledge on the role of MSLN in MPM needs expanding. Accordingly, we investigated the expression of MSLN in a panel of three MPM cell lines, i.e., NCI-H28, Mero-14, and IstMes2; one non-MPM cell line was used as reference (Met5A). MSLN knock-down experiments on MSLN-overexpressing cells were also performed through silencing RNA (siRNA) to verify whether previous findings could be generalized to a different set of cell cultures. In agreement with previous studies, transient MSLN-silencing caused decreased proliferation rate and reduced invasive capacity and sphere formation in MSLN-overexpressing Mero-14 cells. Moreover, MSLN-siRNA combined with cisplatin, triggered a marked increase in apoptosis and a decrease in proliferation as compared to cells treated with each agent alone, thereby suggesting a sensitizing effect of siRNA towards cisplatin. In summary, our findings confirm that MSLN should be considered a key molecular target for novel gene-based targeted therapies of cancer.

Construction and demolition wastes (C&DW) are usually recognized as not dangerous, but their accumulation can generate serious environmental problems. In spite of C&DW high potential to be reused/recycled, the practical procedures need to be assessed in terms of environmental consequences. The objective of this study is to quantify the environmental impacts of C&DW recycling/reuse, specifically in the production of aggregate 0/30 mm, comparative to those generated during the natural inert processing, in terms of global impacts addressing the whole process and for each technological phase. The analysis was carried out using Life Cycle Assessment methodology, assisted by SimaPro software, and based on primary data collected directly from the Italian Emilia Romagna region. Three methods were used for impact quantification: Eco-Indicator 99, EDIP/UMIP and Cumulative Energy Demand. The analysis revealed that the environmental impacts generated by C&DW recycling/reuse accounting for about 40% of the impacts induced by natural inert processing.

O objetivo deste artigo foi revisitar pesquisas que articularam aspectos teórico-metodológicos da Clínica da Atividade de Yves Clot, em particular da autoconfrontação simples e da Pesquisa Colaborativa, no sentido de (re)pensar e transformar a atividade docente em contextos educativos com a presença de um aluno com deficiência. Para isto, utilizamos dados de duas pesquisas desenvolvidas no Programa de Pós-Graduação em Educação da Universidade Federal de Alagoas, com o intuito de ilustrar experiências bem-sucedidas a utilização da pesquisa colaborativa e da autoconfrontação como instrumentos da formação docente para a inclusão. Como resultados pudemos refletir que a ACS e a colaboração foram estratégias metodológicas que viabilizaram a efetiva participação dos professores, nas duas pesquisas, em seu processo de formação. Também identificamos que foi possível que os professores discutissem temas e situações que eram de fato seus anseios e buscassem soluções que fossem adequadas e viáveis para a sua realidade. Por fim, acreditamos que a utilização da autoconfrontação associada a uma abordagem de pesquisa colaborativa pode proporcionar um processo de autoanálise, e possivelmente uma busca por mais formação docente. Entretanto, não foi possível inferir sobre a mudança de concepção e quebra de paradigmas de toda a categoria docente a respeito da inclusão, uma vez que isso depende de vários fatores e não só da postura de um profissional isoladamente.

O nascimento de uma criança com Paralisia Cerebral ocasiona alterações na dinâmica familiar. Deste modo, o objetivo deste estudo é conhecer o que se tem abordado no meio científico sobre as alterações na dinâmica familiar diante do diagnóstico de crianças com paralisia cerebral. Tratou-se de uma revisão de literatura do tipo Estado da Arte, junto às bases de dados LILACS, SciELO e o Banco de Teses e Dissertações da CAPES, com os temos paralisia cerebral e família, no período de 2000 à 2010. Dos 79 artigos encontrados, 18 fizeram parte da amostra. Os artigos analisados mostraram que os cuidadores de crianças com paralisia cerebral que não possuem uma rede de apoio familiar e profissional tendem a apresentar queda na qualidade de vida, nível maior de estresse e quadros de depressão, quando comparados com cuidadores que possuem uma rede de apoio. Também, pode-se observar que a maioria dos cuidadores não conseguiu retomar sua vida acadêmica ou profissional, teve redução de momentos destinados às atividades de lazer, bem como, queda na qualidade do relacionamento conjugal. Diante dos resultados, conclui-se que as mudanças na dinâmica familiar de cuidadores de crianças com paralisia cerebral podem comprometer a saúde mental e a qualidade de vida destes quando não há suporte das redes de apoio.