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Chronic pancreatitis (CP) is a progressive benign fibroinflammatory condition involving repeated episodes of pancreatic inflammation, which lead to fibrotic tissue replacement and subsequent pancreatic insufficiency. A lifetime risk of developing pancreatic ductal adenocarcinoma (PDAC) in patients with chronic pancreatitis is reported to be 1.5%-4%. However, diagnosis of PDAC in patients with CP can be challenging, in part due to overlapping imaging features. In rare instances, pancreatic parenchymal calcifications that are typically associated with chronic pancreatitis may diminish in the case of a developing PDAC. In this article, we present a patient with chronic pancreatitis in whom calcifications decreased at the time of pancreatic ductal adenocarcinoma diagnosis, as compared to prior CT imaging. The unique imaging features of “diminishing calcifications” associated with a hypoattenuating lesion can potentially be a useful sign of pancreatic ductal adenocarcinoma and may aid in early diagnosis and prompt treatment intervention.

Solitary fibrous tumors are rare tumors of pluripotent fibroblastic or myofibroblastic origin that generally arise among older individuals, with a mean age of onset ranging from 55 to 65 years. Though typically associated with pleural involvement, solitary fibrous tumors can emerge in virtually every anatomic location within the body. Although most solitary fibrous tumors are benign, approximately 20% may exhibit malignant features such as local invasion, recurrence, and metastases. In this article, we report the case of a 58-year-old male with a diagnosis of a retroperitoneal solitary fibrous tumor. We analyze computed tomography imaging findings and additionally correlate imaging features with the patient's unique pathological and genotypic findings to optimize diagnosis.

Hepatic angiosarcoma is a rare, highly aggressive mesenchymal liver malignancy with poor prognosis that stems from the endothelial cells that line the walls of blood or lymphatic vessels. It is the third most common primary liver malignancy and is most prevalent among older males. It is difficult to diagnose due to various clinical presentations from asymptomatic to abdominal pain, pleural effusion, and liver failure. The diagnosis of liver angiosarcoma is suspected on imaging features and confirmed by histopathological assessment. Primary management is determined based on the stage of tumor from surgery to palliative care such as chemotherapy or tumor transarterial embolization. We report a 51-year-old female who presented with stage 4 liver angiosarcoma and a history of childhood Wilms tumor. We focus on tumor management using radiological modalities and pathological analysis and discuss secondary liver tumors in survivors of childhood Wilms tumor.

Lymphangioma is a rare, benign congenital malformation of the lymphatic system that usually affects the neck and head in children. Intra-abdominal lymphangioma accounts for less than 5 percent of all cases of lymphangioma. The clinical presentation of intra-abdominal lymphangioma can vary from asymptomatic to nausea, vomiting, and abdominal pain. The diagnosis of intra-abdominal lymphangioma is based on imaging modalities and histopathological examination. The definitive treatment is surgical resection. Here we describe the interesting and rare case of a 29-year-old woman with lymphangioma of the retroperitoneum extending to the root of the mesentery. We focus on the diagnosis and management of this rare tumor by the application of radiological modalities and pathological analysis.

Recurrent acute pancreatitis is characterized by frequent episodes of inflammation in the pancreas. The most common causes are alcohol abuse and gallstones but approximately 30% of cases are defined as idiopathic because initial evaluation fails to detect the etiology. In these patients, extensive laboratory and imaging evaluations usually lead to the uncovering of an occult biliary duct stone or sphincter of Oddi dysfunction as the main reason for frequent pancreatitis. We report a 42-year-old female with a longstanding history of acute recurrent pancreatitis in the setting of pancreas divisum due to its rarity. We focus on appropriate investigations by application of specialized laboratory and radiological modalities because determining the etiology is a crucial step in patient management.

Desmoid tumors are rare, benign, and locally aggressive neoplasms that stem from connective tissue that have high rates of recurrence after surgery. Intra-abdominal desmoid-type fibromatosis can arise in 2 forms: sporadic or hereditary (associated with familial adenomatous polyposis and Gardner syndrome). The diagnosis of desmoid-type tumors is based on imaging modalities and histopathological examination. The primary treatment is resection surgery. We report a 64-year-old male with a distal pancreatic desmoid tumor. We focus on tumor management by the application of radiological modalities and pathological analysis.

Cardiac catheterization through the radial artery has reduced the percentage of complications seen previously in the femoral approach. Computed tomographic angiography is a noninvasive technique that can accurately diagnose pseudoaneurysms and other vascular pathologies.A 93-year-old female presented to the emergency department with pain and swelling of her right distal arm and wrist after a transradial cardiac catheterization procedure, as part of a transcatheter aortic valve replacement evaluation for severe aortic stenosis.Angiography is the standard diagnosing technique for aneurysms and pseudoaneurysms, but it is an invasive procedure. We show this case in order to highlight the importance of computed tomographic angiography as a noninvasive approach in diagnosing uncommon complications in the clinical setting.

En este trabajo se desarrollaron películas híbridas, adaptando el proceso sol-gel con un adecuado control en el diseño de las condiciones experimentales para poder incorporar nanopartículas de sílice a una matriz polimérica de poliestireno reciclado (PSR). Para una mejor incorporación entre la sílice y el PSR fue necesario funcionalizar el PSR con grupos carboxilo de ácido abiético. El PSR usado para la preparación de películas nanohíbridas se obtuvo de productos de empaques de comida rápida, y fue evaluada la citoxicidad de las películas sin sustrato, mostrando la viabilidad de ser usadas como películas protectoras en sustrato de vidrio ya que no presentan riesgo para la salud. Los materiales híbridos fueron caracterizados por las espectroscopias Infrarrojo y Raman, y evaluadas sus propiedades mecánicas y térmicas. La temperatura de transición vítrea se incrementó para los HPS-F and HPSR-F por la presencia del ácido abiético y la sílice. Todos los materiales incrementan su ángulo de contacto comparado con el del sustrato sin recubrir y mayor repelencia al agua. La incorporación de partículas de sílice dentro de la matriz polimérica da por resultado interesantes propiedades comparadas con sus análogas hechas con poliestireno comercial (PS), obteniendo resultados satisfactorios permitiendo así su aplicación como recubrimientos en sustratos de vidrio con propiedades de autolimpieza y protección a la radiación UVA que son de gran relevancia tecnológica.

Reducir la degradación y la deforestación generada principalmente por la ganadería extensiva proporcionando a los ganaderos apoyos con recursos concurrentes entre la Federación y el Gobierno Subnacional para infraestructura, equipo y asistencia técnica especializada a través de un concepto específico que considere los componentes ambientales de otras dependencias y con el fin de establecer Sistemas Silvopastoriles (SSP), es una estrategia de gobernanza que está teniendo resultados positivos en el estado de Jalisco, México. Desde el año 2016, se apoyó a 105 productores para que rediseñaran sus áreas de producción con Sistemas Silvopastoriles, alcanzando una inversión gubernamental de 9.5 millones de pesos mexicanos. Con el apoyo de instituciones académicas, se capacitaron a 110 ganaderos que tienen actividades de conservación o manejo forestal. Se concluye que el cambio de paradigma en las políticas públicas como en los procesos de la producción desde la base social en México debe ser paulatino y de forma gradual, se debe desarrollar la gobernanza local para dar mejor atención a los ganaderos, contar con un marco legal y financiamiento sólido, así como el desarrollo de capacidades con la academia para tener profesionistas preparados en los SSP.

The first case report of human dipylidiasis in Venezuela is presented, including the diagnosis and treatment of a two-year-old child’s infection. The diagnosis was parasitologically confirmed, the child was treated with praziquantel and the animal reservoir and its fleas were identified

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Metabolic changes have been correlated with adverse pregnancy outcomes. The aim of the present study is to determine the TyG and TG/HDL-c indices in a cohort of healthy pregnant (n=142), preeclamptic (n=18), and healthy nonpregnant women (n=56). Preeclamptic women were selected from the same cohort. Pregnant women were followed during three periods of pregnancy and postpartum. The results showed a significant increase in the values of TyG and TG/HDL-c (p<0.01) as pregnancy progresses, without significant differences between healthy and preeclamptic women. TyG and TG/HDL-c indices are significantly low in nonpregnant and three months’ postpartum women when compared with each gestational period studied. TyG and TG/HDL-c indices are positively correlated with HOMA-IR in the early and middle pregnancy (p<0.05). Multiple linear regression using the TyG and TG/HDL-c indices as dependent variables showed that TyG index was significantly associated with HOMA-IR, gestational age, HDL-c, TC, LDL, fasting insulin, and mean BP (p<0.001); meanwhile, TG/HDL-c index was only associated with HOMA-IR (p<0.0242) and gestational age (p<0.001). In conclusion, the TyG and TG/HDL-c indices could be useful in monitoring insulin resistance during pregnancy. [ABSTRACT FROM AUTHOR]

Las competencias ambientales son imprescindibles en el desarrollo de comportamientos que permiten el tratamiento de circunstancias complejas vinculadas al Cambio Climático global. El estudio tuvo el objetivo de identificar el nivel de competencias ambientales que poseen los estudiantes universitarios frente al Cambio Climático. Se configuró la investigación dentro del paradigma positivista, de tipo exploratorio descriptivo, diseño no experimental trasversal. La muestra quedó representada por 180 estudiantes de la carrera educación integral y educación mención computación, de una universidad pública, a este grupo se les aplicó dos cuestionarios. Los resultados evidenciaron que el nivel de competencias ambientales en los estudiantes fueron moderadas, bajas y muy bajas en sus diferentes dimensiones, conceptual, procedimental y actitudinal además de poseer bajos conocimientos sobre las causas y consecuencias del CC. Se concluye que los aprendices no están preparados para enfrentar el CC, por lo que se recomienda incorporar en los planes de la carrera de educación de ambos programas actividades que desarrollen competencias ambientales para la acción que transforme los conocimientos en función de favorecer el cambio de actitudes y patrones de vida que esten alineados a pautas de la Educación para el Desarrollo Sostenible, el cual persigue el empleo adecuado de la energía, el reconocimiento, valor y preservación de los recursos de la naturaleza en pos de la mitigación de los eventos naturales peligrosos vinculados al Cambio Climático, así como también a su adaptación.

El objetivo del estudio fue determinar la influencia de las acciones gerenciales y el rol del docente universitario en el área de investigación en las carreras de Educación de una Universidad Pública Venezolana. Se configuró metodológicamente dentro de un enfoque cuantitativo, tipo descriptivo-correlacional, diseño no experimental transeccional, la muestra quedó representada por 58 docentes del área Ciencias de la Educación. Se empleó la encuesta y dos cuestionarios. Los resultados permitieron identificar que las acciones gerenciales desarrolladas por la coordinación de investigación de la institución son moderadas y bajas, lo que condiciona el logro de objetivos organizacionales con relación al área de investigación, asimismo, se identificó que el rol docente en el área de investigación de la Carrera de Educación, programas educación integral y computación arrojó funciones deficientes, por lo que se requiere de acciones gerenciales que contribuyan a fortalecer el rol del docente en el área de investigación. Por otro lado, se determinó que existe una correlación positiva media entre las variables, por lo tanto, si influye las acciones gerenciales en el rol del docente en el área de investigación, de esta manera es necesario reestructurar y proponer acciones gerenciales en el área de investigación para que el docente sea más competitivo y productivo en el desarrollo de su función como docente investigador.

Turfgrass in equine sports has clear advantages over other types of reinforcement but adds complexity to the management. This study investigates factors that influence the turfgrass’ surface performance and how the use of a drainage package and a geotextile reinforcement affect quantitative measurements of turfgrass. The measurements are made using affordable, lightweight testing tools that are readily available or easily constructed. Eight boxes with turfgrass over a mix of the arena with peat at a consistent depth were tested for volumetric moisture content (VMC %) with time–domain reflectometry (TDR), the rotational peak shear device (RPS), the impact test device (ITD), soil cone penetrometer (SCP), and the Going Stick (GS). Results obtained using TDR, RPS, ITD, SCP, and GS indicate that the presence of the geotextile and drainage package was mainly detected by VMC (%), SCP detected geotextile addition, and GS detected the interaction of geotextile × drainage package. Linear regression showed SCP and GS are related to geotextile and was positively correlated between them and negatively with VMC (%). The testing showed some limitations of these devices, mainly related to moisture content and sod composition, but the potential exists to utilize these devices for quality control as well as for the monitoring of maintenance of the surfaces when controlling the range of both VMC (%) and sod constitution.

Introducción: Cada año mueren seis millones de personas a causa de una lesión traumática, 40% de estas muertes se deben a la hemorragia post-trauma, siendo la primera causa de muerte prevenible a nivel mundial. La presencia y persistencia de la hemorragia conduce a la coagulopatía inducida por trauma (TIC), la cual juega un papel importante en la morbimortalidad de estos pacientes. La detección temprana y tratamiento adecuado de la TIC tiene el potencial de reducir la mortalidad de los pacientes en esta población. Objetivo: Analizar datos de tromboelastografía para determinar perfil de coagulación y correlacionar con uso de hemoderivados y mortalidad. Metodología: Se realizó un estudio retrospectivo con los datos de pacientes post-trauma a quienes se les realizó tromboelastografía en el Hospital General de Accidentes del Instituto Guatemalteco de Seguridad Social del año 2018 al año 2020. Se realizó evaluación y revisión de las variables del tromboelastograma (TEG®), perfil de coagulación por tromboelastografía, mortalidad, ingreso a cuidados intensivos, intervención quirúrgica, tiempo de estancia hospitalaria, cantidad y tipo de hemoderivados utilizados como terapia transfusional. Resultados: 384 pacientes se clasificaron como perfil fibrinolítico, hipocoagulable, hipercoagulable, hemorrágico o normal según los resultados del TEG®. Del total de participantes, el 70.6% presentó alguna alteración de la coagulación, y el 29.4% cumplió criterios de normalidad. La mortalidad promedio en el estudio fue del 32.8%, la mayor reportada en pacientes con perfil hipocoagulable, 49.4%, seguido de hemorrágicos, 37%, hipercoagulables, 28.9%, normales, 27.4%, y finalmente el fibrinolítico, 18.6%. Además, se analizó la terapia transfusional utilizada, siendo la concentración de hematíes la terapia más común con un 61.1%, seguida del plasma fresco congelado con un 28.7% y las plaquetas con un 10.2%. Conclusión: La tromboelastografía es útil para la clasificación del perfil de coagulación del paciente post-trauma y es un estudio de laboratorio que permite determinar los hemoderivados que un paciente traumatizado requiere para reducir la tasa de mortalidad.

En México los paisajes forestales son territorios heterogéneos y complejos. En ellos se desarrollan una multiplicidad de usos que van desde el ecológico, cultural hasta el socioproductivo. Históricamente, las políticas de desarrollo rural y de conservación están desarticuladas, esta situación favorece la degradación y deforestación de la superficie forestal y un desarrollo de sistemas agropecuarios insostenibles. Jalisco, el Gigante Agroalimentario (Padilla et al., 2017) del país, también está examinando los costos ambientales y sociales de tal apelativo (contaminación de aguas, desigualdad, degradación de suelos, deforestación, etc.). La ganadería extensiva los cultivos de agave y aguacate son considerados como impulsores de la deforestación, por lo que se opta por llevar estos sistemas a esquemas libres de deforestación.

Under dysbiosis, a gut metabolic disorder, short-chain carboxylic acids (SCCAs) are secreted to the lumen, affecting colorectal cancer (CRC) development. Butyrate and propionate act as CRC growth inhibitors, but they might also serve as carbon source. In turn, the roles of acetate as metabolic fuel and protein acetylation promoter have not been clearly elucidated. To assess whether acetate favors CRC growth through active mitochondrial catabolism, a systematic study evaluating acetate thiokinase (AcK), energy metabolism, cell proliferation, and invasiveness was performed in two CRC cell lines incubated with physiological SCCAs concentrations. In COLO 205, acetate (+glucose) increased the cell density (50%), mitochondrial protein content (3–10 times), 2-OGDH acetylation, and oxidative phosphorylation (OxPhos) flux (36%), whereas glycolysis remained unchanged vs. glucose-cultured cells; the acetate-induced OxPhos activation correlated with a high AcK activity, content, and acetylation (1.5–6-fold). In contrast, acetate showed no effect on HCT116 cell growth, OxPhos, AcK activity, protein content, and acetylation. However, a substantial increment in the HIF-1α content, HIF-1α-glycolytic protein targets (1–2.3 times), and glycolytic flux (64%) was observed. Butyrate and propionate decreased the growth of both CRC cells by impairing OxPhos flux through mitophagy and mitochondrial fragmentation activation. It is described, for the first time, the role of acetate as metabolic fuel for ATP supply in CRC COLO 205 cells to sustain proliferation, aside from its well-known role as protein epigenetic regulator. The level of AcK determined in COLO 205 cells was similar to that found in human CRC biopsies, showing its potential role as metabolic marker.

Los estudios organizacionales corresponden a las diversas realidades sociales que conciernen a las relaciones e interacciones dinámicas que se presentan entre éstas y el entorno en el que se desenvuelven. Por lo tanto, de acuerdo con el criterio de las investigadoras se plantea como premisa principal entender, comprender y explicar a toda organización, desde el enfoque holístico y sistémico que conforman el todo y el entorno de la organización puesto que se requieren de nuevos paradigmas organizacionales adaptados a la realidad. En tal sentido, se presenta como propósito general analizar desde la visión epistemológica el enfoque holístico y sistémico en los estudios organizacionales. Asimismo, desde la mirada epistemológica de la presente investigación se encuentra contextualizado desde el diseño de investigación documental y del paradigma cualitativo, puesto que el abordaje metodológico de la investigación responde a un determinado enfoque o visión de la realidad, que permitieron realizar un proceso de análisis a través de una matriz ilustrativa que proporcionará las reflexiones en la investigación. Por lo que, como principal reflexión investigativa se resalta que la visión epistemológica de los estudios organizacionales se debe a un proceso de integración entre investigar, comprender, analizar y reflexionar acerca del fenómeno organizacional.

Background and Objectives: Currently, one of the most discouraging aspects for many patients undergoing dental procedures is the administration of anaesthesia. Consequently, there is a constant search for new techniques to avoid the invasive and painful nature of the injection. A new motorised syringe system (Dentapen®) has recently been developed, standing out for its convenience and ease of use. Material and Methods: Randomised, controlled, single-blind, and single-centre study including 178 voluntary adult participants aged between 18 and 90 years. Individuals were randomly assigned using a randomised table. Patients were asked to rate the level of pain experienced during the injections, using a 10-point visual analogue scale (VAS). The following data were recorded: pain index, heart rate, blood pressure, and saturation, both before and after anaesthesia. Results: Of the total 178 participants, 87 participants (48.9%) were men and 91 (51.1%) were women. The first variable to be assessed was the pain experienced by patients when anaesthetised with a syringe, obtaining a mean value of 2.63 ± 1.86 on the VAS with the conventional syringe and 1.06 ± 1.28 with the Dentapen® syringe, showing statistically significant differences (p-value < 0.01). When stratifying, based on the procedure that was undertaken, differences were also significant for all treatments (p-value < 0.01) except for endodontics, where differences were likely to be significant (p-value = 0.02). Conclusions: In conclusion, from a clinical standpoint, the Dentapen® syringe is a valid alternative to traditional infiltration syringes, causing minimum pain with the injection.

Quantitative measurements of performance parameters have the potential to increase consistency and enhance performance of the surfaces as well as to contribute to the safety of horses and riders. This study investigates how factors known to influence the performance of the surface, incorporation of a drainage package, control of the moisture control, and introduction of a geotextile reinforcement, affect quantitative measurements of arena materials. The measurements are made by using affordable lightweight testing tools which are readily available or easily constructed. Sixteen boxes with arena materials at a consistent depth were tested with the Going Stick (GS), both penetration resistance and shear, the impact test device (ITD), and the rotational peak shear device (RPS). Volumetric moisture content (VMC %) was also tested with time–domain reflectometry (TDR). Results obtained using GS, RPS, ITD, and TDR indicate that the presence of the drainage package, moisture content, and geotextile addition were detected. Alterations due to combinations of treatments could also be detected by GS, ITD, and TDR. While the testing showed some limitations of these devices, the potential exists to utilize them for quality control of new installations as well as for the monitoring of maintenance of the surfaces.

The CSF-470 cellular vaccine plus BCG and rhGM-CSF increased distant metastases-free survival in cutaneous melanoma patients stages IIB-IIC-III relative to medium dose IFN-α2b (CASVAC-0401 study). Patient-045 developed a mature vaccination site (VAC-SITE) and a regional cutaneous metastasis (C-MTS), which were excised during the protocol, remaining disease-free 36 months from vaccination start. CDR3-TCRβ repertoire sequencing in PBMC and tissue samples, along with skin-DTH score and IFN-γ ELISPOT assay, were performed to analyze the T-cell immune response dynamics throughout the immunization protocol. Histopathological analysis of the VAC-SITE revealed a highly-inflamed granulomatous structure encircled by CD11c+ nested-clusters, brisk CD8+ and scarce FOXP3+, lymphocytes with numerous Langhans multinucleated-giant-cells and macrophages. A large tumor-regression area fulfilled the C-MTS with brisk lymphocyte infiltration, mainly composed of CD8+PD1+ T-cells, CD20+ B-cells, and scarce FOXP3+ cells. Increasing DTH score and IFN-γ ELISPOT assay signal against the CSF-470 vaccine-lysate was evidenced throughout immunization. TCRβ repertoire analysis revealed for the first time the presence of common clonotypes between a VAC-SITE and a C-MTS; most of them persisted in blood by the end of the immunization protocol. In vitro boost with vaccine-lysate revealed the expansion of persistent clones that infiltrated the VAC-SITE and/or the C-MTS; other persistent clones expanded in the patient's blood as well. We propose that expansion of such persistent clonotypes might derive from two different although complementary mechanisms: the proliferation of specific clones as well as the expansion of redundant clones, which increased the number of nucleotide rearrangements per clonotype, suggesting a functional antigenic selection. In this patient, immunization with the CSF-470 vaccine plus BCG and rhGM-CSF induced a T-cell repertoire at the VAC-SITE that was able to infiltrate an emerging C-MTS, which resulted in the expansion of a T-cell repertoire that persisted in blood by the end of the 2-year treatment.

This study shows that the non-steroidal anti-inflammatory drug (NSAID) celecoxib and its non-cyclooxygenase-2 (COX2) analogue dimethylcelecoxib (DMC) exert a potent inhibitory effect on the growth of human cervix HeLa multi-cellular tumor spheroids (MCTS) when added either at the beginning (“preventive protocol”; IC50 = 1 ± 0.3 nM for celecoxib and 10 ± 2 nM for DMC) or after spheroid formation (“curative protocol”; IC50 = 7.5 ± 2 µM for celecoxib and 32 ± 10 µM for DMC). These NSAID IC50 values were significantly lower than those attained in bidimensional HeLa cells (IC50 = 55 ± 9 µM celecoxib and 48 ± 2 µM DMC) and bidimensional non-cancer cell cultures (3T3 fibroblasts and MCF-10A mammary gland cells with IC50 from 69 to >100 µM, after 24 h). The copper-based drug casiopeina II-gly showed similar potency against HeLa MCTS. Synergism analysis showed that celecoxib, DMC, and casiopeinaII-gly at sub-IC50 doses increased the potency of cisplatin, paclitaxel, and doxorubicin to hinder HeLa cell proliferation through a significant abolishment of oxidative phosphorylation in bidimensional cultures, with no apparent effect on non-cancer cells (therapeutic index >3.6). Similar results were attained with bidimensional human cervix cancer SiHa and human glioblastoma U373 cell cultures. In HeLa MCTS, celecoxib, DMC and casiopeina II-gly increased cisplatin toxicity by 41–85%. These observations indicated that celecoxib and DMC used as adjuvant therapy in combination with canonical anti-cancer drugs may provide more effective alternatives for cancer treatment.

Background and Objectives: Maxillary bone defects related to post-extraction alveolar ridge resorption are usual. These defects may lead to failure in further surgical implant phases given the lack of bone volume to perform the dental implant. The objective of this clinical assay was to evaluate the safety and efficacy of an experimental synthetic bone substitute in the preservation of post-extraction maxillary alveoli. Materials and Methods: 33 voluntary patients who had at least one maxillary premolar tooth that was a candidate for exodontia (n = 39) and subsequent implant rehabilitation participated. The regenerated alveoli were monitored by means of periodic clinical examinations (days 9 ± 1, 21 ± 4, 42 ± 6, and 84 ± 6), measuring the height and width of the alveolar crest (days 0 and 180 ± 5), measurement of radiodensity using tomographic techniques (days 0−5 and 175 ± 5), and histological examination of biopsies collected at 180 ± 5 days. Results: No significant differences were observed during the entire follow-up period between the two groups with respect to the safety variables studied. A variation in width of −0.9 ± 1.3 mm and −0.6 ± 1.5 mm, and a variation in height of −0.1 ± 0.9 mm and −0.3 ± 0.7 mm was observed for experimental material Sil-Oss® and Bio-Oss®, respectively. The radiodensity of the alveoli regenerated with the experimental material was significantly lower than that corresponding to Bio-Oss®. However, the histological study showed greater osteoid matrix and replacement of the material with newformed bone in the implanted beds with the experimental material. Conclusions: Both materials can be used safely and proved equally effective in maintaining alveolar flange dimensions, they are also histologically biocompatible, bioactive and osteoconductive. The experimental material showed the advantage of being resorbable and replaced with newformed bone, in addition to promoting bone regeneration.

The allogeneic therapeutic vaccine CSF-470 has demonstrated a significant benefit over medium-dose IFNα2b in the distant metastasis-free survival for stages IIB–IIC–III cutaneous melanoma patients in a randomized phase II/III clinical trial (CASVAC-0401, NCT01729663). At the end of the 2-year CSF-470 immunization protocol, patient #006 developed several lung and one subcutaneous melanoma metastases; this later was excised. In this report, we analyzed the changes throughout vaccination of immune populations in blood and in the tumor tissue, with special focus on the T-cell repertoire. Immunohistochemistry revealed a marked increase in CD8+, CD4+, and CD20+ lymphocytes infiltrating the metastasis relative to the primary tumor. Lymphocytes were firmly attached to dying-tumor cells containing Granzyme-B granules. Whole-exon sequencing assessment indicated a moderate-to-high tumor mutational burden, with BRAFV600E as the main oncogenic driver. Mutational signature presented large numbers of mutations at dipyrimidines, typical of melanoma. Relevant tumor and immune-related genes from the subcutaneous metastasis were addressed by RNA-Seq analysis, revealing expression of typical melanoma antigens and proliferative tumor-related genes. Stimulatory and inhibitory immune transcripts were detected as well as evidence of active T-cell effector function. Peripheral blood monitoring revealed an increase in CD4+ and CD8+ cells by the end of the immunization protocol. By CDR3-T-cell receptor β (TCRβ) sequencing, generation of new clones and an increase in oligoclonality was observed in the peripheral T-cells immune repertoire throughout immunization. A shift, with the expansion of selected preexisting and newly arising clones with reduction of others, was detected in blood. In tumor-infiltrating lymphocytes, prevalent clones (50%) were both new and preexisting that were expanded in blood following CSF-470 immunization. These clones persisted in time, since 2 years after completing the immunization, 51% of the clones present in the metastasis were still detected in blood. This is the first report of the modulation of the TCRβ repertoire from a melanoma patient immunized with the CSF-470 vaccine. After immunization, the changes observed in peripheral immune populations as well as in the tumor compartment suggest that the vaccine can induce an antitumor adaptive immune repertoire that can reach tumor lesions and persists in blood for at least 2 years.

Metabolic changes have been correlated with adverse pregnancy outcomes. The aim of the present study is to determine the TyG and TG/HDL-c indices in a cohort of healthy pregnant (n=142), preeclamptic (n=18), and healthy nonpregnant women (n=56). Preeclamptic women were selected from the same cohort. Pregnant women were followed during three periods of pregnancy and postpartum. The results showed a significant increase in the values of TyG and TG/HDL-c (p

The irradiated, allogeneic, cellular CSF-470 vaccine plus Bacillus Calmette–Guerin (BCG) and recombinant human granulocyte macrophage-colony stimulating factor (rhGM-CSF) is being tested against medium-dose IFN-α2b in stages IIB–III cutaneous melanoma (CM) patients (pts) after surgery in an open, randomized, Phase II/III study. We present the results of the Phase II part of the ongoing CASVAC-0401 study (ClinicalTrials.gov: NCT01729663). Thirty-one pts were randomized to the CSF-470 vaccine (n = 20) or to the IFN-α2b arm (n = 11). During the 2-year treatment, immunized pts should receive 13 vaccinations. On day 1 of each visit, 1.6 × 107 irradiated CSF-470 cells plus 106 colony-forming units BCG plus 100 µg rhGM-CSF were administered intradermally, followed on days 2–4 by 100 µg rhGM-CSF. IFN-α2b pts should receive 10 million units (MU)/day/5 days a week for 4 weeks; then 5 MU thrice weekly for 23 months. Toxicity and quality of life (QOL) were evaluated at each visit. With a mean and a maximum follow-up of 39.4 and 83 months, respectively, a significant benefit in the distant metastasis-free survival (DMFS) for CSF-470 was observed (p = 0.022). Immune monitoring showed an increase in antitumoral cellular and humoral response in vaccinated pts. CSF-470 was well tolerated; 20/20 pts presented grades 1–2 dermic reactions at the vaccination site; 3/20 pts presented grade 3 allergic reactions. Other adverse events (AEs) were grade 1. Pts in the IFN-α2b arm presented grades 2–3 hematological (7/11), hepatic (2/11), and cardiac (1/11) toxicity; AEs in 9/11 pts forced treatment interruptions. QOL was significantly superior in the vaccine arm (p

El objetivo de este trabajo fue analizar el comportamiento germinativo de semillas dePaspalum dilatatum Poir. provenientes de poblaciones de posición topográfica intermedia y baja y de diferentes historias de uso (clausura y pastoreo). Se utilizaron condiciones de incubación de alternancia de luz y temperatura (20oC 16 hs. oscuridad - 35oC 8 hs.luz) y oscuridad y temperatura constante (25oC). Previo a la incubación la mitad de las semillas se colocaron en condiciones de inmersión, por la relación de estas poblaciones con eventos naturales de inundación. Se estimó la germinación total a través del número de semillas germinadas a los 30 días y la germinación inicial a través de un índice basado en el descripto por Maguire.Tanto la inmersión en agua como la incubación en condiciones de luz y alternancia de temperaturas aumentaron significativamente la germinación inicial y total. La germinación inicial y totaltambién fue afectada por la condición de incubación mostrando en condiciones de luz y alternancia de temperatura valores superiores. La germinación inicial mostró interacciones significativas entre la ubicación topográfica y la condición de incubación y entre esta última y la historia de uso.Las diferencias en la germinación inicial entre condiciones de incubación fueron mayores en las poblaciones deposición topográfica intermedia que en las de posición media y en las provenientes de ambientes pastoreados que en las de clausuras. La interacción entre historia de uso y condición de incubación puede ser explicada por las diferencias en los tamaños de semillas generados en ambientes pastoreados y clausurados respectivamente.