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15 results on '"Albinsson, B"'

1. Site-selective immobilization of functionalized DNA origami on nanopatterned Teflon AF.

Author

Shaali, M., Woller, J. G., Johansson, P. G., Hannestad, J. K., de Battice, L., Aissaoui, N., Brown, T., El-Sagheer, A. H., Kubatkin, S., Lara-Avila, S., Albinsson, B., and Jesorka, A.

Abstract

We demonstrate the use of arrays of Teflon AF nanopillars for directing the assembly of single rectangular DNA origami scaffolds, functionalized with covalently linked fluorophore molecules, in defined positions on patterned surfaces. This is achieved by introducing Teflon AF as a non-amplified negative e-beam resist, which is exposed and chemically developed to generate arrays of hydrophobic nanopillars with a minimum feature size 40 nm. Binding of the DNA origami to the pillars is facilitated by porphyrin moieties that act as hydrophobic molecular anchors, reaching 80% coverage of the available sites. This combination of top-down lithography and bottom-up self assembly is an efficient means of fabricating hierarchically structured bio-nanointerfaces in which the positioning of functional units is precisely controlled on the molecular scale inside the DNA assembly, and on the nanoscale at pre-designed locations on the substrate. [ABSTRACT FROM AUTHOR]

Published
2017
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4. QUADRATURE PHASE SHIFTER USING ONE DUAL-GATE MESFET.

Author

Albinsson, B., Schöön, M., and Vickes, H.-O.

Subjects
*PHASE shifters, *ELECTRONIC equipment, *FIELD-effect transistors, *METAL semiconductor field-effect transistors, *SEMICONDUCTORS
Abstract

Presents a new configuration for a dual-gate quadrature phase shifters. Information on dual-gate field-effect transistors; Analysis; Measurements; Conclusions.

Published
1988
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15. Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging†

Author

Ezequiel Perez-Inestrosa, Shiming Li, Carlos Benitez-Martin, Gaowa Naren, Joakim Andréasson, Wera Larsson, Bo Albinsson, [Naren,G, Larsson,W, Li,S, Albinsson,B, Andréasson,J] Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, Göteborg, Sweden. [Benitez-Martin,C, Pérez-Inestrosa,E] Universidad de Malaga-IBIMA, Departamento de Química Organica, E-29071 Málaga, Spain. [Benitez-Martin,C, Pérez-Inestrosa,E] Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), Malaga, Spain., and Support from the Swedish Research Council VR (Grant # 2016-0360 and Grant # 2018-03998, respectively). E. P.-I. acknowledges the Spanish Ministry for Science, Innovation, and Universities (RD16/0006/0012, PCI2019-111825-2, and PID2019-104293GB-I00), Universidad de Málaga-Junta de Andalucía (UMA18-FEDERJA-007), and FEDER. C. B.-M. is grateful for a FPU fellowship (FPU16/02516).

Subjects
Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Solubility [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Culture Techniques [Medical Subject Headings], Fluorescence-lifetime imaging microscopy, Materials science, Context (language use), Colorantes fluorescentes, 010402 general chemistry, Photochemistry, 01 natural sciences, Fluorescence, Fluorescent dyes, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy::Microscopy, Fluorescence [Medical Subject Headings], 03 medical and health sciences, chemistry.chemical_compound, Rayos ultravioleta, Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Coloring Agents::Fluorescent Dyes [Medical Subject Headings], Diarylethene, Microscopía fluorescente, Fluorescence microscope, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy [Medical Subject Headings], Spectroscopy, Microscopy, fluorescence, 030304 developmental biology, 0303 health sciences, Photoswitch, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Spectrometry, Fluorescence [Medical Subject Headings], Phenomena and Processes::Physical Phenomena::Optical Phenomena::Light [Medical Subject Headings], General Chemistry, Phenomena and Processes::Physical Phenomena::Radiation::Electromagnetic Radiation::Light::Ultraviolet Rays [Medical Subject Headings], UV, 0104 chemical sciences, Autofluorescence, Chemistry, chemistry, Fluorescencia
Abstract

A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy., A water soluble diarylethene derivative displaying exceptionally bright fluorescence in the open isomeric form has been used for emission amplitude-modulation. We apply this scheme in fluorescence microscopy, aiming to suppress undesired background.

Published
2021

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