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Aim: The Thomsen-Friedenreich antigen (TF-Ag) is a disaccharide hidden on normal cells, but selectively exposed on the surface of breast, colon, prostate and bladder cancer cells. JAA-F11, a highly specific monoclonal antibody to TF-Ag, reduces metastasis and prolongs survival in a mouse model. In addition,(124)I-JAA-F11 localizes 4T1 tumors in mice. These studies continue translation of JAA-F11 to human breast cancer. MATERIALS & METHODS & RESULTS: Of the 41 human breast cancer cell lines tested, 78% were positive for reactivity with JAA-F11 by whole-cell enzyme immunoassay and positivity occurred unrelated to estrogen, progesterone or HER2 receptor status. JAA-F11 inhibited the growth rate of the human cancer cell lines tested. At 1 h, approximately 80% of JAA-F11 internalized in the three cell lines tested. (124)I-JAA-F11 specifically imaged human triple-negative tumors in mice by microPET.Conclusion: The results highlight the potential that humanized JAA-F11 may have for immunotherapy and drug conjugate therapy in breast cancer patients. [ABSTRACT FROM AUTHOR]

An in-house loop-mediated isothermal amplification ( LAMP) method was established for the detection of S almonella Typhi and S almonella Paratyphi A using primers that were designed based on gene coding for putative regulatory protein in S. Typhi ( STY4220) and S. Paratyphi A ( SSPA3213). This established in-house LAMP assay gave positive results to all the 14 S. Typhi and 20 S. Paratyphi A isolates used in this study and negative to 20 other S almonella and 19 non- S almonella bacteria. When tested on 60 clinical samples, it gave positive results to 10 clinical samples containing either S. Typhi or S. Paratyphi A and negative to the other 50 samples, which were similar to those results obtained by gold standard culture method. Because of its rapidity and simplicity, this LAMP offers a promising method for the detection of both S. Typhi and S. Paratyphi A for screening purposes at resource-limited settings. Practical Applications Current detection methods for S. Typhi and S. Paratyphi A have several limitations; culture method is time-consuming and laborious, while polymerase chain reaction-based methods require expensive equipments and trained personnel which are usually not available in the low-resource setting. Therefore, LAMP established in this study offers a simple, rapid, sensitive and specific method for the screening of S. Typhi and S. Paratyphi A especially at resource-limited settings. [ABSTRACT FROM AUTHOR]