Search

Pain and inflammation are unpleasant experiences that usually occur as a result of tissue damage. Despite the number of existing analgesic drugs, side effects limit their use, stimulating the search for new therapeutic agents. In this sense, five hydrazone derivatives (H1, H2, H3, H4, and H5), with general structure R1R2C = NNR3R4, were synthesized with molecular modification strategies. In this paper, we describe the ability of hydrazone derivatives to attenuate nociceptive behavior and the inflammatory response in mice. Antinociceptive activity was evaluated through acetic acid-induced writhing and formalin-induced nociception tests. In both experimental models, the hydrazone with the greatest potency (H5) significantly (p < 0.05) reduced nociceptive behavior. Additionally, methods of acute and chronic inflammation induced by different chemicals (carrageenan and histamine) were performed to evaluate the anti-inflammatory effect of H5. Moreover, molecular docking analysis revealed that H5 can block the COX-2 enzyme, reducing arachidonic acid metabolism and consequently decreasing the production of prostaglandins, which are important inflammatory mediators. H5 also changes locomotor activity. In summary, H5 exhibited relevant antinociceptive and anti-inflammatory potential and acted on several targets, making it a candidate for a new multi-target oral anti-inflammatory drug.

The aim of this study was to evaluate the chemical composition, antioxidant and antibacterial activities and cytotoxicity of fractions from Selaginella convoluta, obtained by liquid–liquid extraction using hexane (Sc-Hex), chloroform (Sc-CHCl3) and ethyl acetate (Sc-AcOEt). The phenolic and flavonoid contents were measured by the Folin-Ciocalteu and aluminium chloride methods, respectively. Antioxidant activities were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching test. The antibacterial effect was evaluated by the method of microdilution and the cytotoxicity analysis in HCT-116 (colon), OVCAR-8 (ovarian) and SF-295 (brain) cells were carried out for MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) test. The fractions were positive for the presence of anthracene derivatives, flavonoids, lignans, naphthoquinones, steroids and triterpenoids. The Sc-Hex and Sc-AcOEt showed good antioxidant activities. The fractions of S. convoluta demonstrated antibacterial activity and showed weak cytotoxicity. These activities were correlated with presence of phenolic compounds in active fractions.

Pain and inflammation are complex clinical conditions that are present in a wide variety of disorders. Most drugs used to treat pain and inflammation have potential side effects, which makes it necessary to search for new sources of bioactive molecules. In this paper, we describe the ability of LASSBio-1586, an N-acylhydrazone derivative, to attenuate nociceptive behavior and the inflammatory response in mice. Antinociceptive activity was evaluated through acetic acid-induced writhing and formalin-induced nociception tests. In these experimental models, LASSBio-1586 significantly (p

O estudo objetiva traçar o perfil das Equipes de Saúde da Família (ESF) do município de Petrolina-PE, Brasil, quanto às condições físico-estruturais nas quais se encontram e quanto à qualidade dos serviços de saúde prestados à população, analisando os principais problemas a serem enfrentados pelos profissionais de saúde em seu processo de trabalho. Foi realizado um estudo de caráter observacional descritivo, baseado na aplicação de um questionário semiestruturado, destinado aos profissionais de saúde das mais diversas categorias que trabalham nas ESF do município de Petrolina. Entre os serviços disponibilizados pelo município nas ESF citados pelos profissionais, os considerados mais eficientes foram: vacinação, serviço de enfermagem e pré-natal, enquanto que a farmácia e odontologia foram consideradas os mais precários. A estrutura das unidades foi relatada como o principal problema que dificulta a execução dos serviços nas unidades (43%) e 27% dos profissionais entrevistados afirmaram que as unidades em que trabalham não conseguem atender à demanda. A escassez de profissionais, aliada à falta de equipamentos, materiais e medicamentos, acaba inviabilizando as práticas de trabalho, tornando o sistema de saúde vigente no país defasado, sobrecarregando os macrocomponentes dos níveis de média e alta complexidade em saúde.