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Effects of sludge age on volatile fatty acids (VFAs) production and Phosphorus (P) release during anaerobic acidification of waste activated sludge (WAS) were investigated. Sequencing batch reactors (SBR) fed with simulating domestic sewage were applied to produce WAS of different sludge ages, and batch tests were used for anaerobic acidification. The maximum dissolved total organic carbon, release of PO 4 3 + − P , and accumulation of acetate (C2), propionate (C3), butyrate (C4), and valerate (C5) decreased by 56.2%, 55.8%, 52.6%, 43.7%, 82.4% and 84.8%, respectively, as the sludge age of WAS increased from 5 to 40 days. Limited degradation of protein played a dominating role in decreasing DTOC and VFAs production. Moreover, the increase in molecular weight of organics and organic nitrogen content in the supernatant after acidification suggested that the refractory protein in WAS increased as sludge age extended. Although the production of C2, C3, C4, and C5 from WAS decreased as the sludge age increased, the proportions of C2 and C3 in VFAs increased, which might be due to the declined production of C5 from protein and the faded genus Dechlorobacter. Keeping sludge age of WAS at a relatively low level (<10 days) is more appropriate for anaerobic acidification of WAS as internal carbon sources and P resource. [Display omitted] [ABSTRACT FROM AUTHOR]

• Extracellular protein and carbohydrate increased and declined by adding particles. • Secondary structure of protein and MW of EPS changed little by silica particles. • Degradation of protein and polysaccharide increased and declined as particles added. • Promotion in protein degradation mainly contributed to the increased VS conversion. • Extracellular organics amounts in unit VS mainly correlated to degradation of sludge. The influence of micron-sized silica particles on the distribution of organic matters in sludge and anaerobic degradation of macromolecular organic components (MOCs) in sludge was investigated. With the addition of micron-sized particles in the influent (VS/TS decreased gradually from 90.46 ± 0.21 % to 33.36 ± 0.17 %), the protein degradation percentage was significantly promoted while the polysaccharides degradation percentage was largely inhibited, resulting in the total MOCs degradation and methane production increasing firstly (with the promotion extent within 10 %) and then declining slightly, with the peak value at VS/TS of 56.03 ± 0.21 %. The shifted degradation percentage of protein and polysaccharides were caused by the significant changed distribution of organic matters in sludge. With the addition of micron-sized silica particles, the MW of EPS and secondary structure of protein in EPS changed little, which brought about little influence. While, the promoted extracellular protein content (so that the total protein content) and declined extracellular carbohydrates content (so that the total polysaccharides content), were found to be strongly correlated to the enhanced protein degradation and inhibited polysaccharides degradation. The results suggested that large amounts of grit in sludge might not be the main reason for lower degradability of sewage sludge in China. [ABSTRACT FROM AUTHOR]

[Display omitted] • A single stage PN/A process treating low strength wastewater was started rapidly. • The biofilm growth process on porous polyurethane hydrogel carrier was studied. • Biomass entrapment by interconnected micropores dominated the initial colonization. • AOB was surface-attached growth, anammox colonies proliferated as embedded growth. Media-supported biofilm is a powerful strategy for growth and enrichment of slow-growing microorganisms. In this study, a single-stage nitritation-anammox process treating low-strength wastewater was successfully started to investigate the biofilm development on porous polyurethane hydrogel carrier. Suspended biomass migration into the carrier and being entrapment by its internal interconnected micropores dominated the fast initial colonization stage. Both surface-attached growth and embedded growth of microbes occurred during the following accumulation stage. Fluorescence in situ hybridization analysis of mature biofilm indicated that ammonium-oxidizing bacteria located at the outer layers featured a surface-attached growth, while anammox microcolonies housed in the inner layers proliferated as an embedded-like growth. In this way, the growth rate of anammox bacteria (predominated by Candidatus Kuenenia) could be 0.079 d-1. The anammox potential of the biofilm reactor reached 1.65 ± 0.3 kg/m3/d within two months. This study provides novel insights into nitritation-anammox biofilm formation on the porous polyurethane hydrogel carrier. [ABSTRACT FROM AUTHOR]

Achieving mainstream nitritation with aerobic granules is attractive based on increasing evidence but generally treating artificial low-ammonium wastewater. Real municipal wastewater is much more complex in composition, the behavior of the nitritation granules would be different when treating real municipal wastewater. Herein, the response of nitritation granules to influent shift from artificial low-ammonium (35–40 mg/L) wastewater to anaerobically pre-treated municipal wastewater (MWW pre-treated) was investigated at low temperatures. Results showed that MWW pre-treated caused the outgrowth of filamentous bacteria on the granule surface and developed into finger-like structures, which in turn resulted in the decrease of the overall granular sludge settleability. Batch-tests and microbial analysis indicated the functional and microbial differentiation between the newly formed fluffy exterior and the original compact granule. The fluffy exterior was dominated by genus Flavobacterium (66.6%) and primarily functioned as COD removal, whereas the nitrifiers (mainly Nitrosomonas) were still located in the compact core and performed nitritation. Moreover, the heterotrophs-dominated fluffy exterior hindered the oxygen transfer towards nitrifiers located in the compact granule and thereby facilitated the stable NOB repression in the granule particularly at low temperatures (<10 °C). Finally, gradual recovery of the granular sludge morphology and settleability occurred after the influent reverted to synthetic low-ammonium wastewater. Overall, this work demonstrated that the feeding of MWW pre-treated only caused morphological changes of the nitritation granules, but its structural and functional stability could be maintained stably. [Display omitted] • Granule-based nitritation was maintained treating anaerobically pretreated sewage. • COD removal occurred primarily in external flocs, while nitritation in compact core. • External flocs promoted NOB repression by increasing oxygen transfer resistance. [ABSTRACT FROM AUTHOR]

Biomass segregation between granules/biofilm and flocs is widespread in anammox-based processes. The segregation of biomass allows for easy control of processes stability. The goal of this study is to understand the biomass segregation in two anoxic anammox reactors respectively operated in nitrite-limited (R NO2) and ammonium-limited (R NH4) modes treating low-strength wastewater at 20 °C. Results showed that size-based biomass segregation was developed in both reactors. But the functional and population heterogeneity was more significant in the ammonium-limited anammox reactor. The activity and abundance of anammox bacteria in large granules were significantly higher than that in flocs under the ammonium-limited conditions. The large granules played a major role in nitrogen removal in R NH4. By contrast, both large granules and small flocs contributed significantly to the nitrogen loss in the nitrite-limited anammox reactor, since a large number of anammox bacteria existed in both granules and flocs. Besides, a number of Nitrospira -like NOB were also detected in both anoxic anammox reactors, which primarily inhabited in flocs seemingly droved by the availability of oxygen. But the abundance of Nitrospira in R NH4 was much higher than that in R NO2. All these results suggested that selective flocs removal would be necessary for R NH4 to improve its anammox performance but non-essential for R NO2. The two anammox reactors shared the predominant anammox species with the closest relative to Ca. Brocadia sp. 40 (98%). Unexpectedly, the anammox species grew faster in R NH4. But the microbial diversity and evenness was much greater in R NO2 , suggesting its higher functional stability. • The ammonium-limited and nitrite-limited anammox reactors were compared. • Anammox bacteria mainly enriched in granules, NOB preferred to live in flocs. • Anammox bacteria grew faster in the ammonium-limited reactor. • The nitrite-limited reactor possessed the higher microbial diversity and evenness. [ABSTRACT FROM AUTHOR]