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In this study, settling tests were conducted to investigate the sedimentation and self-weight consolidation behavior of seafloor sediments from Isahaya Bay, Ariake Sea, Japan. During the tests, the density variations with depth and time were measured by a gamma-ray transmission radioisotope densitometer. The test results show that the settling process of the seafloor sediments can be classified into the flocculation stage, settling stage, and consolidation stage. The settling rate of the seafloor sediments in the settling stage is dependent on the temperature and initial water content, while the settling rate in the consolidation stage is independent of the temperature and initial water content. The density profile changes from a constant density profile to a linear density profile when the sedimentation process transitions to the self-weight consolidation process. The relations between the void ratio (e) and effective vertical stress (p') at very low pressures can be calculated from the measured density values, and this can be used for the analysis of the self-weight consolidation of seafloor sediments. For the seafloor sediments tested in this study, the undrained shear strength (su) values are almost the same when the density values are less than 1.14 g/cm3, and the su values increase linearly with an increase in density when the density values are in the range of 1.14–1.2 g/cm3. [ABSTRACT FROM AUTHOR]