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1. Corrigendum to 'Rotating-disc micro-solid phase extraction of F2-isoprostanes from maternal and cord plasma by using oxidized buckypaper as sorbent membrane' [J. Chromatogr. A 1586 (2019) 30–39] (Journal of Chromatography A (2019) 1586 (30–39), (S0021967318315188), (10.1016/j.chroma.2018.12.011))

Author

Tomai, P., Martinelli, A., Gasperi, T., Bianchi, Maria, Purcaro, Velia, Teofili, Luciana, Papacci, Patrizia, Cori, M. S., Vento, Giovanni, Curini, R., Fanali, S., and Gentili, Andrea

Subjects
Paper, F2-Isoprostanes, Nanotubes, Biological samples, LC–MS, Sample preparation, Solid phase extraction, Adsorption, Female, Fetal Blood, Humans, Infant, Newborn, Limit of Detection, Nanotubes, Carbon, Pregnancy, Solid Phase Extraction, Solvents, Tandem Mass Spectrometry, Buckypaper, Carbon nanotubes, Infant, Newborn, Carbon, LC-MS, Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA, Samples preparation
Published
2019

2. Rotating-disc micro-solid phase extraction of F2-isoprostanes from maternal and cord plasma by using oxidized buckypaper as sorbent membrane

Author

Roberta Curini, Salvatore Fanali, Velia Purcaro, Giovanni Vento, Patrizia Papacci, Tecla Gasperi, Maria Bianchi, Pierpaolo Tomai, Andrea Martinelli, Alessandra Gentili, Maria Sofia Cori, Luciana Teofili, Tomai, Pierpaolo, Martinelli, Andrea, Gasperi, Tecla, Bianchi, Maria, Purcaro, Velia, Teofili, Luciana, Papacci, Patrizia, Cori, Maria Sofia, Vento, Giovanni, Curini, Roberta, Fanali, Salvatore, and Gentili, Alessandra

Subjects
Paper, Analyte, Buckypaper, Calibration curve, Biological samples, Carbon nanotubes, LC–MS, Sample preparation, Solid phase extraction, Adsorption, F2-Isoprostanes, Female, Fetal Blood, Humans, Infant, Newborn, Limit of Detection, Nanotubes, Carbon, Pregnancy, Solid Phase Extraction, Solvents, Tandem Mass Spectrometry, 010402 general chemistry, 01 natural sciences, Biochemistry, Carbon nanotube, Analytical Chemistry, Liquid chromatography–mass spectrometry, Biological sample, Nanotubes, Chromatography, Chemistry, Elution, 010401 analytical chemistry, Organic Chemistry, Extraction (chemistry), Infant, General Medicine, Newborn, biological samples, buckypaper, carbon nanotubes, sample preparation, solid phase extraction, analytical chemistry, biochemistry, organic chemistry, Carbon, 0104 chemical sciences, Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA, Quantitative analysis (chemistry)
Abstract

This paper describes the development of an original micro-solid phase extraction device and its evaluation for the isolation of F2-isoprostanes (F2-IsoPs) from cord and maternal plasma samples. The unit is very simple and consists in a rotating disc (1.8 cm diameter) of oxidized buckypaper (BP), enwrapped in a polypropylene mesh pouch. Even if the selected F2-IsoPs have logP and pKa values that make them suitable candidates for their sorption on BP, several parameters were optimized to maximize recoveries: time of adsorption and desorption; stirring speed; volume, pH and ionic strength of the sample; type, volume, and fractions of the elution solvent; oxidation grade of BP. Among all, the last one was crucial in affecting extraction yields because of the analyte interactions with polar functionalities, introduced by a preliminary oxidative acid treatment. The investigation established the optimal oxidation time and highlighted the pros and cons of the acid activation step. All extracts were analyzed by means of liquid chromatography-tandem mass spectrometry (LC–MS/MS). Validation was performed according to the main FDA guidelines for bioanalytical methods. Depending on the spike level and analyte, recoveries ranged between 30 and 120% with precision and accuracy values lower than 20%. Quantitative analysis was accomplished by matrix-matched calibration curves whose determination coefficients were higher than 0.95. Lower limit of quantitation (LLOQ) spanned the range 2.45–6.77 μg L−1. The validated method was applied to the analysis of eight pairs of mother/child plasma samples, revealing the presence of 8-iso-15-keto-PGF2α and 8-iso-PGE2 at a concentration of about 10 μg L−1 in most cord plasma samples of preterm newborns.

Published
2018