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Husk tomato ( Physalis ixocarpa Brot. var. Rendidora) waste was evaluated as a source of specialized pectin, and pectin extracted from this waste was characterized physicochemically. Fruit was blanched for 10 or 15 min and extracted in 0.1 N HCl for 15 to 25 min. Extracted pectin was subjected to physicochemical analysis. For all extraction conditions, the percentage of anhydrogalacturonic acid exceeded 60%, indicating that husk tomato was a good source of pectin. The degree of esterification of pectin molecules was 63% to 91%. The amount of extracted pectin decreased with increasing extraction time. The apparent viscosity of husk tomato pectin showed the characteristic behavior of pseudoplastic fluids. Neutral sugars were identified, and the amounts of 6 sugars (fucose, rhamnose, arabinose, galactose, glucose, and xylose) were quantified. Sugars identified in husk tomato pectin and present in the Rhamnogalacturonan I region, arabinose, galactose, and rhamnose suggest a highly branched structure, which will influence its future applications. Molecular weight values were 542 to 699 kDa, exceeding molecular weight values reported for commercial citrus pectins from 134 to 480 kDa. The extraction process significantly ( P < 0.05) influenced the physicochemical properties of pectin. Up to 19.8% from the total amount of pectin in the husk tomato was extracted by 10 min of blanching and 20 min of a more heat treatment. Our findings indicate that husk tomato can be a good alternative source of pectin having highly distinctive physicochemical characteristics. [ABSTRACT FROM AUTHOR]

Sugar Beet Pectin (BP) was sequentially fractionated with increasing isopropanol into six fractions from a commercial BP control (BP2–C) and denoted as BP2–F1 to BP2–F6. Fractions of BP2 were categorized based on physico-chemical characteristics of similar protein and ferulic acid content, varying galacturonic acid, surface hydrophobicity, degree of esterification and molecular weight. Glycan-directed monoclonal antibodies response showed intense binding for homogalacturonan, rhamnogalacturonan I and arabinogalactan epitopes that varied between early and later precipitating fractions, and between lots of BP. In a 14-day study of rice bran oil-in-water emulsion, the particle size (2 ± 0.3 μm) and ζ-potential (−34 ± 3 mV) remained stable for BP2–F3 and B2–F4. Fractions that precipitated at lower alcohol concentrations likely have more amphiphilic properties, compared to later precipitating fractions that provide more steric stabilization. [Display omitted] • Sequential isopropanol fractionation of beet pectin (BP) resulted in two populations. • Emulsifying stability was associated with amphiphilic BP and steric stabilization. • Glycome profiling provided comprehensive view of cell wall glycan epitopes. • Glycome profiles were unique for BP controls and fractions. [ABSTRACT FROM AUTHOR]