1. Fluazifop-P-butyl induced ROS generation with IAA (indole-3-acetic acid) oxidation in Acanthospermum hispidum D.C.
- Author
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Liu, Zhihang, Li, Pingliang, Sun, Xiaoxue, Zhou, Fei, Yang, Congjun, Li, Lingxu, Matsumoto, Hiroshi, and Luo, Xiaoyong
- Subjects
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FLUAZIFOP-p-butyl , *INDOLEACETIC acid , *OXIDATION , *REACTIVE oxygen species , *HERBICIDES , *SHOOT apical meristems - Abstract
Acanthospermum hispidum D.C. was particularly susceptible to fluazifop-P-butyl, an aryloxyphenoxypropionate herbicide, and the primary action site for the herbicide was shoot apical meristem, which is also the main site of indole-3-acetic acid (IAA) biosynthesis and action. Membrane lipid peroxidation caused by increasing levels of reactive oxygen species (ROS) was considered as an action mechanism of fluazifop-P-butyl in A. hispidum . To further clarify the ROS inducing mechanism of fluazifop-P-butyl in the plant, the interactions between fluazifop-P-butyl and auxin compounds IAA or 2,4-dichlorophenoxyacetic acid (2,4-D) were studied. Haloxyfop- P -methyl, an AOPP herbicide which is inactive on A. hispidum , was used for comparison. The results showed that the growth inhibition and malondialdehyde or H 2 O 2 increases induced by fluazifop-P-butyl on A. hispidum were reversed by IAA or 2,4-D. The IAA content was decreased but the contents of three IAA oxidation metabolites, indole-3-methanol, indole-3-aldehyde and indole-3-carboxylic acid were increased by fluazifop-P-butyl in A. hispidum , but not by haloxyfop- P -methyl. The growth of A. hispidum was not inhibited by three IAA oxidative compounds. Moreover, the activities of IAA oxidase and peroxidase were increased by fluazifop-P-butyl but not by haloxyfop- P -methyl, and the increase was reversed by IAA or 2,4-D. We suggest that there is an antagonistic effect between fluazifop-P-butyl and IAA or 2,4-D, and the IAA oxidation may be involved in the action mechanism of fluazifop-P-butyl in A. hispidum . [ABSTRACT FROM AUTHOR]
- Published
- 2017
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