7 results on '"LaRocque, Regina C."'
Search Results
2. Human Gut Microbiota Predicts Susceptibility to Vibrio cholerae Infection.
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Midani, Firas S, Weil, Ana A, Chowdhury, Fahima, Begum, Yasmin A, Khan, Ashraful I, Debela, Meti D, Durand, Heather K, Reese, Aspen T, Nimmagadda, Sai N, Silverman, Justin D, Ellis, Crystal N, Ryan, Edward T, Calderwood, Stephen B, Harris, Jason B, Qadri, Firdausi, David, Lawrence A, and LaRocque, Regina C
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PATHOGENIC microorganisms ,COMMUNICABLE diseases ,VIBRIO cholerae ,INFECTION ,MEDICAL microbiology ,MICROBIOLOGY - Abstract
Background: Cholera is a public health problem worldwide, and the risk factors for infection are only partially understood.Methods: We prospectively studied household contacts of patients with cholera to compare those who were infected to those who were not. We constructed predictive machine learning models of susceptibility, using baseline gut microbiota data. We identified bacterial taxa associated with susceptibility to Vibrio cholerae infection and tested these taxa for interactions with V. cholerae in vitro.Results: We found that machine learning models based on gut microbiota, as well as models based on known clinical and epidemiological risk factors, predicted V. cholerae infection. A predictive gut microbiota of roughly 100 bacterial taxa discriminated between contacts who developed infection and those who did not. Susceptibility to cholera was associated with depleted levels of microbes from the phylum Bacteroidetes. By contrast, a microbe associated with cholera by our modeling framework, Paracoccus aminovorans, promoted the in vitro growth of V. cholerae. Gut microbiota structure, clinical outcome, and age were also linked.Conclusion: These findings support the hypothesis that abnormal gut microbial communities are a host factor related to V. cholerae susceptibility. [ABSTRACT FROM AUTHOR]- Published
- 2018
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3. Global TravEpiNet: A National Consortium of Clinics Providing Care to International Travelers-Analysis of Demographic Characteristics, Travel Destinations, and Pretravel Healthcare of High-Risk US International Travelers, 2009-2011.
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LaRocque, Regina C., Rao, Sowmya R., Lee, Jennifer, Ansdell, Vernon, Yates, Johnnie A., Schwartz, Brian S., Knouse, Mark, Cahill, John, Hagmann, Stefan, Vinetz, Joseph, Connor, Bradley A., Goad, Jeffery A., Oladele, Alawode, Alvarez, Salvador, Stauffer, William, Walker, Patricia, Kozarsky, Phyllis, Paredes, Carlos Franco, Dismukes, Roberta, and Rosen, Jessica
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MEDICAL care , *DISEASE risk factors , *COMMUNICABLE diseases , *YELLOW fever vaccines , *EPIDEMIOLOGY - Abstract
Background. International travel poses a risk of destination-specific illness and may contribute to the global spread of infectious diseases. Despite this, little is known about the health characteristics and pretravel healthcare of US international travelers, particularly those at higher risk of travel-associated illness. Methods. We formed a national consortium (Global TravEpiNet) of 18 US clinics registered to administer yellow fever vaccination. We collected data regarding demographic and health characteristics, destinations, purpose of travel, and pretravel healthcare from 13 235 international travelers who sought pretravel consultation at these sites from January 2009 through January 2011. Results. The destinations and itineraries of Global TravEpiNet travelers differed from those of the overall population of US international travelers. The majority of Global TravEpiNet travelers were visiting low- or lower-middle-income countries, and Africa was the most frequently visited region. Seventy-five percent of travelerswere visitingmalaria-endemic countries, and 38% were visiting countries endemic for yellow fever. Fifty-nine percent of travelers reported ≤1 medical condition. Atovaquone/proguanil was the most commonly prescribed antimalarial drug, and most travelers received an antibiotic for self-treatment of travelers' diarrhea. HepatitisA and typhoid were themost frequently administered vaccines. Conclusions. Data from Global TravEpiNet provide insight into the characteristics and pretravel healthcare of US international travelers who are at increased risk of travel-associated illness due to itinerary, purpose of travel, or existing medical conditions. Improved understanding of this epidemiologically significant population may help target risk-reduction strategies and interventions to limit the spread of infections related to global travel [ABSTRACT FROM AUTHOR]
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- 2012
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4. High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic.
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Sealfon, Rachel, Gire, Stephen, Ellis, Crystal, Calderwood, Stephen, Qadri, Firdausi, Hensley, Lisa, Kellis, Manolis, Ryan, Edward T., LaRocque, Regina C., Harris, Jason B., and Sabeti, Pardis C.
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GENOMES ,MICROBIAL evolution ,EPIDEMICS ,VIBRIO infections ,COMMUNICABLE diseases - Abstract
Background: Whole-genome sequencing is an important tool for understanding microbial evolution and identifying the emergence of functionally important variants over the course of epidemics. In October 2010, a severe cholera epidemic began in Haiti, with additional cases identified in the neighboring Dominican Republic. We used whole-genome approaches to sequence four Vibrio cholerae isolates from Haiti and the Dominican Republic and three additional V. cholerae isolates to a high depth of coverage (>2000x); four of the seven isolates were previously sequenced. Results: Using these sequence data, we examined the effect of depth of coverage and sequencing platform on genome assembly and identification of sequence variants. We found that 50x coverage is sufficient to construct a whole-genome assembly and to accurately call most variants from 100 base pair paired-end sequencing reads. Phylogenetic analysis between the newly sequenced and thirty-three previously sequenced V. cholerae isolates indicates that the Haitian and Dominican Republic isolates are closest to strains from South Asia. The Haitian and Dominican Republic isolates form a tight cluster, with only four variants unique to individual isolates. These variants are located in the CTX region, the SXT region, and the core genome. Of the 126 mutations identified that separate the Haiti-Dominican Republic cluster from the V. cholerae reference strain (N16961), 73 are non-synonymous changes, and a number of these changes cluster in specific genes and pathways. Conclusions: Sequence variant analyses of V. cholerae isolates, including multiple isolates from the Haitian outbreak, identify coverage-specific and technology-specific effects on variant detection, and provide insight into genomic change and functional evolution during an epidemic. [ABSTRACT FROM AUTHOR]
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- 2012
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5. Individuals with Le(a+b-) Blood Group Have Increased Susceptibility to Symptomatic Vibrio cholerae O1 Infection.
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Arifuzzaman, Mohammad, Ahmed, Tanvir, Rahman, Mohammad Arif, Chowdhury, Fahima, Rashu, Rasheduzzaman, Khan, Ashraful I., LaRocque, Regina C., Harris, Jason B., Bhuiyan, Taufiqur Rahman, Ryan, Edward T., Calderwood, Stephen B., and Qadri, Firdausi
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VIBRIO cholerae ,DISEASE susceptibility ,BLOOD group antigens ,COMMUNICABLE diseases ,LEWIS blood group system ,CHOLERA ,IMMUNE response - Abstract
Background: Human genetic factors such as blood group antigens may affect the severity of infectious diseases. Presence of specific ABO and Lewis blood group antigens has been shown previously to be associated with the risk of different enteric infections. The aim of this study was to determine the relationship of the Lewis blood group antigens with susceptibility to cholera, as well as severity of disease and immune responses to infection. Methodology: We determined Lewis and ABO blood groups of a cohort of patients infected by Vibrio cholerae O1, their household contacts, and healthy controls, and analyzed the risk of symptomatic infection, severity of disease if infected and immune response following infection. Principal Findings: We found that more individuals with cholera expressed the Le(a+b-) phenotype than the asymptomatic household contacts (OR 1.91, 95% CI 1.03-3.56) or healthy controls (OR 1.90, 95% CI 1.13-3.21), as has been seen previously for the risk of symptomatic ETEC infection. Le(a-b+) individuals were less susceptible to cholera and if infected, required less intravenous fluid replacement in hospital, suggesting that this blood group may be associated with protection against V. cholerae O1. Individuals with Le(a-b-) blood group phenotype who had symptomatic cholera had a longer duration of diarrhea and required higher volumes of intravenous fluid replacement. In addition, individuals with Le(a-b-) phenotype also had lessened plasma IgA responses to V. cholerae O1 lipopolysaccharide on day 7 after infection compared to individuals in the other two Lewis blood group phenotypes. Conclusion: Individuals with Lewis blood type Le(a+b-) are more susceptible and Le(a-b+) are less susceptible to V. cholerae O1 associated symptomatic disease. Presence of this histo-blood group antigen may be included in evaluating the risk for cholera in a population, as well as in vaccine efficacy studies, as is currently being done for the ABO blood group antigens. [ABSTRACT FROM AUTHOR]
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- 2011
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6. Transmission of Vibrio cholerae Is Antagonized by Lytic Phage and Entry into the Aquatic Environment.
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Nelson, Eric J., Chowdhury, Ashrafuzzaman, Flynn, James, Schild, Stefan, Bourassa, Lori, Shao, Yue, LaRocque, Regina C., Calderwood, Stephen B., Qadri, Firdausi, and Camilli, Andrew
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EPIDEMICS ,VIBRIO cholerae ,CHOLERA ,COMMUNICABLE diseases ,PHENOTYPES - Abstract
Cholera outbreaks are proposed to propagate in explosive cycles powered by hyperinfectious Vibrio cholerae and quenched by lytic vibriophage. However, studies to elucidate how these factors affect transmission are lacking because the field experiments are almost intractable. One reason for this is that V. cholerae loses the ability to culture upon transfer to pond water. This phenotype is called the active but non-culturable state (ABNC; an alternative term is viable but non-culturable) because these cells maintain the capacity for metabolic activity. ABNC bacteria may serve as the environmental reservoir for outbreaks but rigorous animal studies to test this hypothesis have not been conducted. In this project, we wanted to determine the relevance of ABNC cells to transmission as well as the impact lytic phage have on V. cholerae as the bacteria enter the ABNC state. Rice-water stool that naturally harbored lytic phage or in vitro derived V. cholerae were incubated in a pond microcosm, and the culturability, infectious dose, and transcriptome were assayed over 24 h. The data show that the major contributors to infection are culturable V. cholerae and not ABNC cells. Phage did not affect colonization immediately after shedding from the patients because the phage titer was too low. However, V. cholerae failed to colonize the small intestine after 24 h of incubation in pond water-the point when the phage and ABNC cell titers were highest. The transcriptional analysis traced the transformation into the non-infectious ABNC state and supports models for the adaptation to nutrient poor aquatic environments. Phage had an undetectable impact on this adaptation. Taken together, the rise of ABNC cells and lytic phage blocked transmission. Thus, there is a fitness advantage if V. cholerae can make a rapid transfer to the next host before these negative selective pressures compound in the aquatic environment. [ABSTRACT FROM AUTHOR]
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- 2008
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7. Complexity of rice-water stool from patients with Vibrio cholerae plays a role in the transmission of infectious diarrhea.
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Nelson, Eric J., Chowdhury, Ashrafuzzaman, Harris, Jason B., Begumt, Yasmin A., Chowdhury, Fahima, Khan, Ashraful I., LaRocque, Regina C., Bishop, Anne L., Ryan, Edward T., Camilli, Andrew, Qadri, Firdausi, and Calderwood, Stephen B.
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VIBRIO cholerae ,DIARRHEA ,BACTERIOPHAGES ,MUCINS ,COMMUNICABLE diseases ,MEDICAL research - Abstract
At the International Centre for Diarrhoeal Disease Research. Bangladesh, one-half of the rice-water stool samples that were culture-positive for Vibrio cholerae did not contain motile V. cholerae by standard darkfield microscopy and were defined as darkfield-negative (DF-). We evaluated the host and microbial factors associated with DF status, as well as the impact of DF status on transmission. Viable counts of V. cholerae in DF stools were three logs lower than in DF- stools, although DF and DF- stools had similar direct counts of V. cholerae by microscopy. In DF- samples, non-V. cholerae bacteria outnumbered V. cholerae 10:1. Lytic V. cholerae bacteriophage were present in 90% of DF- samples compared with 35% of DF- samples, suggesting that bacterio-phage may limit culture-positive patients from producing DF- stools. V. cholerae in DF and DF- samples were found both planktonically and in distinct nonplanktonic populations; the distribution of organisms between these compartments did not differ appreciably between DF- and DF4 stools. This biology may impact transmission because epidemiological data suggested that house-hold contacts of a DF- index case were at greater risk of infection with V. cholerae. We propose a model in which V. cholerae multiply in the small intestine to produce a fluid niche that is dominated by V. cholerae. If lytic phage are present, viable counts of V. cholerae drop, stools become DF, other microorganisms bloom, and cholera transmission is reduced. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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