1. Discovery of a Structural-Element Specific G-Quadruplex ''Light-Up'' Probe.
- Author
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Liyun Zhang, Jun Cheng Er, Ghosh, Krishna Kanta, Wan Jun Chung, Jaeduk Yoo, Wang Xu, Wei Zhao, Anh Tuân Phan, and Young-Tae Chang
- Subjects
FLUORESCENT probes ,QUADRUPLEX nucleic acids ,AQUEOUS solutions ,FLUORESCENCE spectroscopy ,BIOLOGICAL research - Abstract
The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and four medium grooves. Mechanistic studies suggest that GQR associates with G-quadruplex grooves close to the end of the tetrad core, which may explain the dye's specificity to only a subset of parallel structures. This specific recognition favours the disaggregation of GQR in aqueous solutions thereby recovering the inherent fluorescence of the dye. Due to its unique features, GQR represents a valuable tool for basic biological research and the rapid discovery of novel, specific ligands that target similar structural features of G-quadruplexes [ABSTRACT FROM AUTHOR]
- Published
- 2014
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