1. Filter paper-based spin column method for cost-efficient DNA or RNA purification
- Author
-
Rui Shi, Ramsey S. Lewis, and Dilip R. Panthee
- Subjects
0106 biological sciences ,0301 basic medicine ,Nicotiana ,Paper ,Leaves ,DNA, Plant ,DNA purification ,Molecular biology ,Science ,Equipment ,Artificial Gene Amplification and Extension ,Centrifugation ,Plant Science ,Molecular biology assays and analysis techniques ,01 natural sciences ,Purification techniques ,Biochemistry ,Polymerase Chain Reaction ,03 medical and health sciences ,Filter Paper ,Solanum lycopersicum ,Nucleic Acids ,Tobacco ,Particle Spin ,DNA filter assay ,Particle Physics ,Flowering Plants ,Multidisciplinary ,Physics ,Plant Anatomy ,Organisms ,Biology and Life Sciences ,Eukaryota ,Plants ,Research and analysis methods ,Laboratory Equipment ,030104 developmental biology ,Molecular biology techniques ,RNA, Plant ,Physical Sciences ,Medicine ,Engineering and Technology ,010606 plant biology & botany ,Research Article - Abstract
We describe herein a method of recharging used commercial spin columns or assembling homemade spin columns using filter paper as binding material for cost-effective, low throughput nucleic acid purification. The efficiency of filter paper-based spin columns was evaluated for purification of nucleic acids from various sources. Following protocols of commercial kits, we found filter paper to be a useful binding material for purification of nucleic acids, including plant genomic DNA, plant total RNA, PCR products, and DNA from agarose gels. However, filter paper has a weak binding affinity to plasmid DNA in tested miniprep protocols. Protocols for the use of filter paper recharged spin columns or homemade spin columns for low throughput purification of plant genomic DNA and total RNA with unused commercial kit buffers or less expensive homemade buffers are presented.
- Published
- 2018